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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 5 |
| AclWI | GGATC | 1 cut(s) | 56 |
| AcoI | YGGCCR | 1 cut(s) | 21 |
| AfaI | GTAC | 1 cut(s) | 186 |
| AfeI | AGCGCT | 1 cut(s) | 63 |
| AluBI | AGCT | 3 cut(s) | 37, 76, 99 |
| AluI | AGCT | 3 cut(s) | 37, 76, 99 |
| Alw26I | GTCTC | 1 cut(s) | 24 |
| AlwI | GGATC | 1 cut(s) | 56 |
| Aor51HI | AGCGCT | 1 cut(s) | 63 |
| AoxI | GGCC | 1 cut(s) | 21 |
| AspLEI | GCGC | 1 cut(s) | 64 |
| BanI | GGYRCC | 1 cut(s) | 5 |
| BbsI | GAAGAC | 1 cut(s) | 206 |
| BcoDI | GTCTC | 1 cut(s) | 24 |
| BfoI | RGCGCY | 1 cut(s) | 65 |
| BmiI | GGNNCC | 2 cut(s) | 7, 109 |
| BpiI | GAAGAC | 1 cut(s) | 206 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 21, 53 |
| BpuEI | CTTGAG | 1 cut(s) | 134 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 154, 184 |
| Bse118I | RCCGGY | 1 cut(s) | 23 |
| BseX3I | CGGCCG | 1 cut(s) | 21 |
| Bsh1285I | CGRYCG | 1 cut(s) | 24 |
| BshFI | GGCC | 1 cut(s) | 23 |
| BshNI | GGYRCC | 1 cut(s) | 5 |
| BsiEI | CGRYCG | 1 cut(s) | 24 |
| BsiSI | CCGG | 1 cut(s) | 24 |
| BsmAI | GTCTC | 1 cut(s) | 24 |
| BsnI | GGCC | 1 cut(s) | 23 |
| Bsp143I | GATC | 1 cut(s) | 48 |
| BspANI | GGCC | 1 cut(s) | 23 |
| BspLI | GGNNCC | 2 cut(s) | 7, 109 |
| BspPI | GGATC | 1 cut(s) | 56 |
| BspT107I | GGYRCC | 1 cut(s) | 5 |
| BsrFI | RCCGGY | 1 cut(s) | 23 |
| BssAI | RCCGGY | 1 cut(s) | 23 |
| BssMI | GATC | 1 cut(s) | 48 |
| Bst4CI | ACNGT | 1 cut(s) | 17 |
| Bst6I | CTCTTC | 1 cut(s) | 130 |
| BstC8I | GCNNGC | 2 cut(s) | 60, 101 |
| BstH2I | RGCGCY | 1 cut(s) | 65 |
| BstHHI | GCGC | 1 cut(s) | 64 |
| BstKTI | GATC | 1 cut(s) | 51 |
| BstMAI | GTCTC | 1 cut(s) | 24 |
| BstMBI | GATC | 1 cut(s) | 48 |
| BstMCI | CGRYCG | 1 cut(s) | 24 |
| BstV2I | GAAGAC | 1 cut(s) | 206 |
| BstZI | CGGCCG | 1 cut(s) | 21 |
| BsuRI | GGCC | 1 cut(s) | 23 |
| Cac8I | GCNNGC | 2 cut(s) | 60, 101 |
| CfoI | GCGC | 1 cut(s) | 64 |
| Cfr10I | RCCGGY | 1 cut(s) | 23 |
| Csp6I | GTAC | 1 cut(s) | 185 |
| CviJI | RGCY | 4 cut(s) | 23, 37, 76, 99 |
| CviKI_1 | RGCY | 4 cut(s) | 23, 37, 76, 99 |
| CviQI | GTAC | 1 cut(s) | 185 |
| DpnI | GATC | 1 cut(s) | 50 |
| DpnII | GATC | 1 cut(s) | 48 |
| EaeI | YGGCCR | 1 cut(s) | 21 |
| EagI | CGGCCG | 1 cut(s) | 21 |
| Eam1104I | CTCTTC | 1 cut(s) | 130 |
| EarI | CTCTTC | 1 cut(s) | 130 |
| EclXI | CGGCCG | 1 cut(s) | 21 |
| Eco47III | AGCGCT | 1 cut(s) | 63 |
| Eco52I | CGGCCG | 1 cut(s) | 21 |
| GlaI | GCGC | 1 cut(s) | 63 |
| HaeII | RGCGCY | 1 cut(s) | 65 |
| HaeIII | GGCC | 1 cut(s) | 23 |
| HapII | CCGG | 1 cut(s) | 24 |
| HhaI | GCGC | 1 cut(s) | 64 |
| Hin6I | GCGC | 1 cut(s) | 62 |
| HinP1I | GCGC | 1 cut(s) | 62 |
| HinfI | GANTC | 2 cut(s) | 125, 202 |
| HpaII | CCGG | 1 cut(s) | 24 |
| Hpy188I | TCNGA | 4 cut(s) | 41, 48, 80, 130 |
| HpyAV | CCTTC | 1 cut(s) | 183 |
| HpyCH4III | ACNGT | 1 cut(s) | 17 |
| HpyCH4V | TGCA | 1 cut(s) | 58 |
| HspAI | GCGC | 1 cut(s) | 62 |
| Kzo9I | GATC | 1 cut(s) | 48 |
| LmnI | GCTCC | 3 cut(s) | 42, 81, 175 |
| LpnPI | CCDG | 1 cut(s) | 37 |
| MalI | GATC | 1 cut(s) | 50 |
| MboI | GATC | 1 cut(s) | 48 |
| MboII | GAAGA | 2 cut(s) | 147, 211 |
| MluCI | AATT | 1 cut(s) | 215 |
| MlyI | GAGTC | 1 cut(s) | 196 |
| MnlI | CCTC | 2 cut(s) | 19, 205 |
| MseI | TTAA | 1 cut(s) | 95 |
| MspI | CCGG | 1 cut(s) | 24 |
| NdeII | GATC | 1 cut(s) | 48 |
| NlaIV | GGNNCC | 2 cut(s) | 7, 109 |
| PfeI | GAWTC | 1 cut(s) | 125 |
| PleI | GAGTC | 1 cut(s) | 196 |
| PpsI | GAGTC | 1 cut(s) | 196 |
| PspN4I | GGNNCC | 2 cut(s) | 7, 109 |
| RsaI | GTAC | 1 cut(s) | 186 |
| RsaNI | GTAC | 1 cut(s) | 185 |
| SaqAI | TTAA | 1 cut(s) | 95 |
| Sau3AI | GATC | 1 cut(s) | 48 |
| SchI | GAGTC | 1 cut(s) | 196 |
| SetI | ASST | 4 cut(s) | 39, 78, 87, 101 |
| SmlI | CTYRAG | 1 cut(s) | 113 |
| SmoI | CTYRAG | 1 cut(s) | 113 |
| Sse9I | AATT | 1 cut(s) | 215 |
| TaaI | ACNGT | 1 cut(s) | 17 |
| TasI | AATT | 1 cut(s) | 215 |
| TatI | WGTACW | 1 cut(s) | 184 |
| TfiI | GAWTC | 1 cut(s) | 125 |
| Tru1I | TTAA | 1 cut(s) | 95 |
| Tru9I | TTAA | 1 cut(s) | 95 |
| TspDTI | ATGAA | 1 cut(s) | 165 |