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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 105 |
| AciI | CCGC | 2 cut(s) | 309, 324 |
| AfaI | GTAC | 1 cut(s) | 71 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 143, 163, 323 |
| AgsI | TTSAA | 3 cut(s) | 341, 361, 367 |
| AluBI | AGCT | 1 cut(s) | 120 |
| AluI | AGCT | 1 cut(s) | 120 |
| AoxI | GGCC | 2 cut(s) | 53, 145 |
| AspS9I | GGNCC | 3 cut(s) | 53, 145, 187 |
| AsuC2I | CCSGG | 1 cut(s) | 52 |
| AvaII | GGWCC | 1 cut(s) | 187 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 24, 56 |
| BccI | CCATC | 2 cut(s) | 233, 326 |
| BcnI | CCSGG | 1 cut(s) | 52 |
| BfaI | CTAG | 1 cut(s) | 162 |
| BfmI | CTRYAG | 1 cut(s) | 105 |
| BfuAI | ACCTGC | 1 cut(s) | 105 |
| BisI | GCNGC | 1 cut(s) | 325 |
| BlsI | GCNGC | 1 cut(s) | 326 |
| Bme1390I | CCNGG | 1 cut(s) | 52 |
| Bme18I | GGWCC | 1 cut(s) | 187 |
| BmgT120I | GGNCC | 3 cut(s) | 53, 145, 187 |
| BmiI | GGNNCC | 2 cut(s) | 146, 257 |
| BmrFI | CCNGG | 1 cut(s) | 52 |
| BpuMI | CCSGG | 1 cut(s) | 52 |
| BsaJI | CCNNGG | 1 cut(s) | 183 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 143, 163, 323 |
| BseDI | CCNNGG | 1 cut(s) | 183 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 143, 163, 323 |
| BsgI | GTGCAG | 1 cut(s) | 83 |
| BshFI | GGCC | 2 cut(s) | 55, 147 |
| BsiSI | CCGG | 1 cut(s) | 51 |
| BsiWI | CGTACG | 1 cut(s) | 69 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 143, 163, 323 |
| BsnI | GGCC | 2 cut(s) | 55, 147 |
| BspACI | CCGC | 2 cut(s) | 309, 324 |
| BspANI | GGCC | 2 cut(s) | 55, 147 |
| BspLI | GGNNCC | 2 cut(s) | 146, 257 |
| BspMI | ACCTGC | 1 cut(s) | 105 |
| BssECI | CCNNGG | 1 cut(s) | 183 |
| BssT1I | CCWWGG | 1 cut(s) | 183 |
| Bst4CI | ACNGT | 1 cut(s) | 241 |
| Bst6I | CTCTTC | 1 cut(s) | 362 |
| BstC8I | GCNNGC | 1 cut(s) | 134 |
| BstNSI | RCATGY | 2 cut(s) | 89, 136 |
| BstSCI | CCNGG | 1 cut(s) | 50 |
| BstSFI | CTRYAG | 1 cut(s) | 105 |
| BsuRI | GGCC | 2 cut(s) | 55, 147 |
| BtsIMutI | CAGTG | 1 cut(s) | 237 |
| BveI | ACCTGC | 1 cut(s) | 105 |
| Cac8I | GCNNGC | 1 cut(s) | 134 |
| Cfr13I | GGNCC | 3 cut(s) | 53, 145, 187 |
| Csp6I | GTAC | 1 cut(s) | 70 |
| CviAII | CATG | 5 cut(s) | 57, 86, 133, 158, 272 |
| CviJI | RGCY | 5 cut(s) | 50, 55, 120, 147, 327 |
| CviKI_1 | RGCY | 5 cut(s) | 50, 55, 120, 147, 327 |
| CviQI | GTAC | 1 cut(s) | 70 |
| Eam1104I | CTCTTC | 1 cut(s) | 362 |
| EarI | CTCTTC | 1 cut(s) | 362 |
| Eco130I | CCWWGG | 1 cut(s) | 183 |
| Eco47I | GGWCC | 1 cut(s) | 187 |
| EcoT14I | CCWWGG | 1 cut(s) | 183 |
| ErhI | CCWWGG | 1 cut(s) | 183 |
| FaeI | CATG | 5 cut(s) | 60, 89, 136, 161, 275 |
| FaiI | YATR | 8 cut(s) | 17, 58, 87, 107, 134, 159, 273, 379 |
| FatI | CATG | 5 cut(s) | 56, 85, 132, 157, 271 |
| FauI | CCCGC | 1 cut(s) | 316 |
| Fnu4HI | GCNGC | 1 cut(s) | 325 |
| Fsp4HI | GCNGC | 1 cut(s) | 325 |
| FspBI | CTAG | 1 cut(s) | 162 |
| GluI | GCNGC | 1 cut(s) | 325 |
| HaeIII | GGCC | 2 cut(s) | 55, 147 |
| HapII | CCGG | 1 cut(s) | 51 |
| Hin1II | CATG | 5 cut(s) | 60, 89, 136, 161, 275 |
| HinfI | GANTC | 3 cut(s) | 205, 287, 348 |
| HpaII | CCGG | 1 cut(s) | 51 |
| Hpy188I | TCNGA | 2 cut(s) | 220, 292 |
| Hpy188III | TCNNGA | 1 cut(s) | 248 |
| Hpy99I | CGWCG | 1 cut(s) | 306 |
| HpyCH4III | ACNGT | 1 cut(s) | 241 |
| HpyCH4V | TGCA | 2 cut(s) | 19, 100 |
| Hsp92II | CATG | 5 cut(s) | 60, 89, 136, 161, 275 |
| LpnPI | CCDG | 4 cut(s) | 64, 110, 215, 261 |
| MaeI | CTAG | 1 cut(s) | 162 |
| MaeIII | GTNAC | 1 cut(s) | 171 |
| MboII | GAAGA | 3 cut(s) | 44, 47, 349 |
| MluCI | AATT | 1 cut(s) | 212 |
| MlyI | GAGTC | 1 cut(s) | 342 |
| MmeI | TCCRAC | 1 cut(s) | 103 |
| MnlI | CCTC | 6 cut(s) | 73, 138, 208, 219, 290, 365 |
| MspI | CCGG | 1 cut(s) | 51 |
| MspR9I | CCNGG | 1 cut(s) | 52 |
| NciI | CCSGG | 1 cut(s) | 52 |
| NlaIII | CATG | 5 cut(s) | 60, 89, 136, 161, 275 |
| NlaIV | GGNNCC | 2 cut(s) | 146, 257 |
| NspI | RCATGY | 2 cut(s) | 89, 136 |
| PaeI | GCATGC | 1 cut(s) | 136 |
| PfeI | GAWTC | 2 cut(s) | 205, 287 |
| Pfl23II | CGTACG | 1 cut(s) | 69 |
| PkrI | GCNGC | 1 cut(s) | 326 |
| PleI | GAGTC | 1 cut(s) | 342 |
| PpsI | GAGTC | 1 cut(s) | 342 |
| PspLI | CGTACG | 1 cut(s) | 69 |
| PspN4I | GGNNCC | 2 cut(s) | 146, 257 |
| PspPI | GGNCC | 3 cut(s) | 53, 145, 187 |
| RsaI | GTAC | 1 cut(s) | 71 |
| RsaNI | GTAC | 1 cut(s) | 70 |
| SatI | GCNGC | 1 cut(s) | 325 |
| Sau96I | GGNCC | 3 cut(s) | 53, 145, 187 |
| SchI | GAGTC | 1 cut(s) | 342 |
| ScrFI | CCNGG | 1 cut(s) | 52 |
| SetI | ASST | 5 cut(s) | 99, 122, 200, 257, 357 |
| SfcI | CTRYAG | 1 cut(s) | 105 |
| SinI | GGWCC | 1 cut(s) | 187 |
| SphI | GCATGC | 1 cut(s) | 136 |
| Sse9I | AATT | 1 cut(s) | 212 |
| SsiI | CCGC | 2 cut(s) | 309, 324 |
| SspMI | CTAG | 1 cut(s) | 162 |
| StyD4I | CCNGG | 1 cut(s) | 50 |
| StyI | CCWWGG | 1 cut(s) | 183 |
| TaaI | ACNGT | 1 cut(s) | 241 |
| TaqI | TCGA | 2 cut(s) | 91, 351 |
| TasI | AATT | 1 cut(s) | 212 |
| TauI | GCSGC | 1 cut(s) | 327 |
| TfiI | GAWTC | 2 cut(s) | 205, 287 |
| TscAI | CASTG | 1 cut(s) | 244 |
| TspDTI | ATGAA | 2 cut(s) | 17, 288 |
| TspRI | CASTG | 1 cut(s) | 244 |
| VpaK11BI | GGWCC | 1 cut(s) | 187 |
| XceI | RCATGY | 2 cut(s) | 89, 136 |
| XspI | CTAG | 1 cut(s) | 162 |