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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 3 cut(s) | 95, 149, 166 |
| AcsI | RAATTY | 1 cut(s) | 213 |
| AgsI | TTSAA | 1 cut(s) | 59 |
| AluBI | AGCT | 2 cut(s) | 21, 64 |
| AluI | AGCT | 2 cut(s) | 21, 64 |
| ApeKI | GCWGC | 1 cut(s) | 21 |
| ApoI | RAATTY | 1 cut(s) | 213 |
| AspS9I | GGNCC | 1 cut(s) | 192 |
| AsuHPI | GGTGA | 1 cut(s) | 194 |
| AvaII | GGWCC | 1 cut(s) | 192 |
| BbvI | GCAGC | 1 cut(s) | 8 |
| BisI | GCNGC | 1 cut(s) | 22 |
| BlsI | GCNGC | 1 cut(s) | 23 |
| Bme18I | GGWCC | 1 cut(s) | 192 |
| BmgT120I | GGNCC | 1 cut(s) | 192 |
| Bse1I | ACTGG | 1 cut(s) | 203 |
| BseMII | CTCAG | 1 cut(s) | 79 |
| BseNI | ACTGG | 1 cut(s) | 203 |
| BseRI | GAGGAG | 1 cut(s) | 113 |
| BseXI | GCAGC | 1 cut(s) | 8 |
| BspACI | CCGC | 3 cut(s) | 95, 149, 166 |
| BspCNI | CTCAG | 1 cut(s) | 78 |
| BspQI | GCTCTTC | 1 cut(s) | 123 |
| BsrI | ACTGG | 1 cut(s) | 203 |
| Bst4CI | ACNGT | 1 cut(s) | 111 |
| Bst6I | CTCTTC | 2 cut(s) | 123, 147 |
| BstDEI | CTNAG | 2 cut(s) | 65, 162 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 70 |
| BstV1I | GCAGC | 1 cut(s) | 8 |
| BtsI | GCAGTG | 1 cut(s) | 220 |
| BtsIMutI | CAGTG | 1 cut(s) | 220 |
| Cfr13I | GGNCC | 1 cut(s) | 192 |
| CviJI | RGCY | 3 cut(s) | 21, 64, 202 |
| CviKI_1 | RGCY | 3 cut(s) | 21, 64, 202 |
| DdeI | CTNAG | 2 cut(s) | 65, 162 |
| Eam1104I | CTCTTC | 2 cut(s) | 123, 147 |
| EarI | CTCTTC | 2 cut(s) | 123, 147 |
| EciI | GGCGGA | 1 cut(s) | 110 |
| Eco47I | GGWCC | 1 cut(s) | 192 |
| FaiI | YATR | 1 cut(s) | 190 |
| FauI | CCCGC | 1 cut(s) | 159 |
| Fnu4HI | GCNGC | 1 cut(s) | 22 |
| Fsp4HI | GCNGC | 1 cut(s) | 22 |
| GluI | GCNGC | 1 cut(s) | 22 |
| HinfI | GANTC | 1 cut(s) | 232 |
| HphI | GGTGA | 1 cut(s) | 194 |
| Hpy188I | TCNGA | 1 cut(s) | 68 |
| HpyCH4III | ACNGT | 1 cut(s) | 111 |
| HpyCH4V | TGCA | 1 cut(s) | 24 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 70 |
| HpyF3I | CTNAG | 2 cut(s) | 65, 162 |
| LguI | GCTCTTC | 1 cut(s) | 123 |
| LpnPI | CCDG | 5 cut(s) | 46, 119, 211, 216, 221 |
| Lsp1109I | GCAGC | 1 cut(s) | 8 |
| MaeIII | GTNAC | 3 cut(s) | 28, 111, 205 |
| MboII | GAAGA | 2 cut(s) | 140, 164 |
| MluCI | AATT | 1 cut(s) | 213 |
| MnlI | CCTC | 3 cut(s) | 91, 96, 228 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 70 |
| NmuCI | GTSAC | 2 cut(s) | 28, 205 |
| PciSI | GCTCTTC | 1 cut(s) | 123 |
| PfeI | GAWTC | 1 cut(s) | 232 |
| PkrI | GCNGC | 1 cut(s) | 23 |
| PspPI | GGNCC | 1 cut(s) | 192 |
| SapI | GCTCTTC | 1 cut(s) | 123 |
| SatI | GCNGC | 1 cut(s) | 22 |
| Sau96I | GGNCC | 1 cut(s) | 192 |
| SetI | ASST | 2 cut(s) | 23, 66 |
| SgeI | CNNG | 9 cut(s) | 45, 65, 146, 179, 211, 215, 220, 238, 248 |
| SinI | GGWCC | 1 cut(s) | 192 |
| Sse9I | AATT | 1 cut(s) | 213 |
| SsiI | CCGC | 3 cut(s) | 95, 149, 166 |
| TaaI | ACNGT | 1 cut(s) | 111 |
| TasI | AATT | 1 cut(s) | 213 |
| TfiI | GAWTC | 1 cut(s) | 232 |
| TscAI | CASTG | 1 cut(s) | 227 |
| TseFI | GTSAC | 2 cut(s) | 28, 205 |
| TseI | GCWGC | 1 cut(s) | 21 |
| Tsp45I | GTSAC | 2 cut(s) | 28, 205 |
| TspDTI | ATGAA | 1 cut(s) | 159 |
| TspGWI | ACGGA | 1 cut(s) | 224 |
| TspRI | CASTG | 1 cut(s) | 227 |
| VpaK11BI | GGWCC | 1 cut(s) | 192 |
| XapI | RAATTY | 1 cut(s) | 213 |