Rroxscaffold_2G00120690

Extra-large guanine nucleotide-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0007563
Physical Location & Seq
Forward (+)
53021844 .. 53023034
1191 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00120690.1

Sequence Viewer

Length: 759 bp
ATGGCTGCTGCGGTTATGAGGAAGCCGAGTGCTGCTTTGGCTTCAAAAGGTGCTGATTTTGATGTCGAGAGTTACGAGGATTTGAGCGCGGAGTTGGAATACTCGTTTGCGGTGGAGTATGAAGGTCCTCCGGTGGGTTATGAAATCCCACATGCGAAGGCCGGAGTAGAGACTAAACTTGGGGATGAAGTAAAGGCGGTTCTTGATTCGGGTGAAGAGAAGGGTGTGTGTAGTGAAGTTGGGAGCTCGGTGAATTGGGTTGTGGAAAGTTGGAGGGTGAGGTGGAGTTGTATGAGGATGGTGGAGAAGGTGATTGGGGATCAACGAGTCCGGGTTGAGCTCAAGGAGTGTTTCATCCGAGGTTTTCTCGGCAAAGAGGAAGCCGGTGTGGATGAGATTGTTCCTCACCATGTGAAAAGGCCATCGACCGTGACTTTTCGCGATCCGGATTCAAATGACATATTCAAGGACTGGCCTTTCAAGGACCATGTGGAGATTTTTAATAAGATTATGGGATTTTTCATGCGGTTTGAAGATGATAGTGTAGAGTCGGTAAAGATTTTGAAGCGGTGGAATGTGAAGAACTACTATGCGTCGGTGAGGGGTTTCAAGGACGAGTTGTTGTGGGCAGCTTTATTGTTATACGAGGCCACCGACAATGAAGAGTATTTGAGATACATCGAAGTAGAAGCTCTTGAGGAGAAGTTAATGTCGTTGCTTGGTGTTGAAGGACATATAGGAGAATGGAGTGGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0001664 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0005102 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005834 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009898 GO:0009987 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019897 GO:0019898 GO:0023052 GO:0031234 GO:0031683 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0035639 GO:0036094 GO:0042742 GO:0043167 GO:0043168 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0044877 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071944 GO:0097159 GO:0097367 GO:0098542 GO:0098552 GO:0098562 GO:0098796 GO:0098797 GO:1901265 GO:1901363 GO:1902494 GO:1905360
KEGG Pathways
Metabolic & Signaling
ko04015 ko04022 ko04024 ko04062 ko04071 ko04261 ko04360 ko04371 ko04540 ko04611 ko04670 ko04713 ko04723 ko04724 ko04725 ko04726 ko04727 ko04728 ko04730 ko04914 ko04915 ko04916 ko04921 ko04923 ko04924 ko04926 ko04934 ko04971 ko05012 ko05030 ko05032 ko05034 ko05133 ko05142 ko05145 ko05200 map04015 map04022 map04024 map04062 map04071 map04261 map04360 map04371 map04540 map04611 map04670 map04713 map04723 map04724 map04725 map04726 map04727 map04728 map04730 map04914 map04915 map04916 map04921 map04923 map04924 map04926 map04934 map04971 map05012 map05030 map05032 map05034 map05133 map05142 map05145 map05200
Pfam Domains
Protein Families
G-alpha

Protein Analysis

252

Amino Acids

28.66

Weight (kDa)

4.8

Isoelectric Point (pI)

38.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC73_C PF05179 154 - 196 1.8e-07 RNA pol II accessory factor, Cdc73 family, C-terminal
Glyco_hydro_9 PF00759 193 - 233 6.5e-08 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 89, 441
AccIII TCCGGA 1 cut(s) 445
AciI CCGC 6 cut(s) 11, 89, 110, 197, 526, 568
AclWI GGATC 2 cut(s) 327, 437
AdeI CACNNNGTG 1 cut(s) 412
AfiI CCNNNNNNNGG 1 cut(s) 134
AgsI TTSAA 8 cut(s) 45, 453, 466, 481, 533, 565, 610, 728
AluBI AGCT 5 cut(s) 246, 340, 632, 692, 755
AluI AGCT 5 cut(s) 246, 340, 632, 692, 755
Alw21I GWGCWC 2 cut(s) 248, 342
Alw26I GTCTC 1 cut(s) 164
AlwI GGATC 2 cut(s) 327, 437
Aor13HI TCCGGA 1 cut(s) 445
AoxI GGCC 4 cut(s) 159, 419, 473, 648
ApeKI GCWGC 4 cut(s) 5, 8, 32, 629
AspLEI GCGC 1 cut(s) 89
AspS9I GGNCC 2 cut(s) 125, 484
AsuC2I CCSGG 1 cut(s) 332
AsuHPI GGTGA 6 cut(s) 224, 262, 289, 322, 398, 610
AvaII GGWCC 2 cut(s) 125, 484
BanII GRGCYC 2 cut(s) 248, 342
Bbv12I GWGCWC 2 cut(s) 248, 342
BbvI GCAGC 2 cut(s) 19, 641
BccI CCATC 2 cut(s) 292, 430
BcnI CCSGG 1 cut(s) 332
BcoDI GTCTC 1 cut(s) 164
BisI GCNGC 4 cut(s) 6, 9, 33, 630
BlsI GCNGC 4 cut(s) 7, 10, 34, 631
Bme1390I CCNGG 1 cut(s) 332
Bme18I GGWCC 2 cut(s) 125, 484
BmgT120I GGNCC 2 cut(s) 125, 484
BmrFI CCNGG 1 cut(s) 332
BplI GAGNNNNNCTC 2 cut(s) 351, 383
BpuEI CTTGAG 2 cut(s) 326, 716
BpuMI CCSGG 1 cut(s) 332
BsaJI CCNNGG 1 cut(s) 358
BsaWI WCCGGW 2 cut(s) 130, 445
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse118I RCCGGY 1 cut(s) 383
Bse1I ACTGG 1 cut(s) 476
BseAI TCCGGA 1 cut(s) 445
BseDI CCNNGG 1 cut(s) 358
BseGI GGATG 4 cut(s) 190, 303, 354, 397
BseLI CCNNNNNNNGG 1 cut(s) 134
BseNI ACTGG 1 cut(s) 476
BseRI GAGGAG 1 cut(s) 713
BseXI GCAGC 2 cut(s) 19, 641
Bsh1236I CGCG 2 cut(s) 89, 441
Bsh1285I CGRYCG 1 cut(s) 429
BshFI GGCC 4 cut(s) 161, 421, 475, 650
BsiEI CGRYCG 1 cut(s) 429
BsiHKAI GWGCWC 2 cut(s) 248, 342
BsiSI CCGG 5 cut(s) 131, 162, 331, 384, 446
BslI CCNNNNNNNGG 1 cut(s) 134
BsmAI GTCTC 1 cut(s) 164
BsnI GGCC 4 cut(s) 161, 421, 475, 650
Bsp1286I GDGCHC 2 cut(s) 248, 342
Bsp13I TCCGGA 1 cut(s) 445
Bsp143I GATC 2 cut(s) 319, 442
Bsp68I TCGCGA 1 cut(s) 441
BspACI CCGC 6 cut(s) 11, 89, 110, 197, 526, 568
BspANI GGCC 4 cut(s) 161, 421, 475, 650
BspEI TCCGGA 1 cut(s) 445
BspFNI CGCG 2 cut(s) 89, 441
BspPI GGATC 2 cut(s) 327, 437
BsrFI RCCGGY 1 cut(s) 383
BsrI ACTGG 1 cut(s) 476
BssAI RCCGGY 1 cut(s) 383
BssECI CCNNGG 1 cut(s) 358
BssMI GATC 2 cut(s) 319, 442
Bst4CI ACNGT 1 cut(s) 430
Bst6I CTCTTC 2 cut(s) 210, 657
BstF5I GGATG 4 cut(s) 190, 303, 354, 397
BstFNI CGCG 2 cut(s) 89, 441
BstHHI GCGC 1 cut(s) 89
BstKTI GATC 2 cut(s) 322, 445
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 2 cut(s) 319, 442
BstMCI CGRYCG 1 cut(s) 429
BstMWI GCNNNNNNNGC 1 cut(s) 38
BstNSI RCATGY 1 cut(s) 155
BstSCI CCNGG 1 cut(s) 330
BstUI CGCG 2 cut(s) 89, 441
BstV1I GCAGC 2 cut(s) 19, 641
BsuRI GGCC 4 cut(s) 161, 421, 475, 650
BtsCI GGATG 4 cut(s) 190, 303, 354, 397
BtuMI TCGCGA 1 cut(s) 441
CfoI GCGC 1 cut(s) 89
Cfr10I RCCGGY 1 cut(s) 383
Cfr13I GGNCC 2 cut(s) 125, 484
CseI GACGC 1 cut(s) 582
CviAII CATG 4 cut(s) 152, 410, 488, 523
DpnI GATC 2 cut(s) 321, 444
DpnII GATC 2 cut(s) 319, 442
DraIII CACNNNGTG 1 cut(s) 412
Eam1104I CTCTTC 2 cut(s) 210, 657
EarI CTCTTC 2 cut(s) 210, 657
Ecl136II GAGCTC 2 cut(s) 246, 340
Eco24I GRGCYC 2 cut(s) 248, 342
Eco47I GGWCC 2 cut(s) 125, 484
Eco53kI GAGCTC 2 cut(s) 246, 340
EcoICRI GAGCTC 2 cut(s) 246, 340
EcoO109I RGGNCCY 1 cut(s) 125
EcoT38I GRGCYC 2 cut(s) 248, 342
FaeI CATG 4 cut(s) 155, 413, 491, 526
FatI CATG 4 cut(s) 151, 409, 487, 522
Fnu4HI GCNGC 4 cut(s) 6, 9, 33, 630
FokI GGATG 4 cut(s) 197, 310, 341, 404
FriOI GRGCYC 2 cut(s) 248, 342
Fsp4HI GCNGC 4 cut(s) 6, 9, 33, 630
GlaI GCGC 1 cut(s) 88
GluI GCNGC 4 cut(s) 6, 9, 33, 630
HaeIII GGCC 4 cut(s) 161, 421, 475, 650
HapII CCGG 5 cut(s) 131, 162, 331, 384, 446
HgaI GACGC 1 cut(s) 582
HhaI GCGC 1 cut(s) 89
Hin1II CATG 4 cut(s) 155, 413, 491, 526
Hin6I GCGC 1 cut(s) 87
HinP1I GCGC 1 cut(s) 87
HinfI GANTC 4 cut(s) 206, 327, 449, 548
HpaII CCGG 5 cut(s) 131, 162, 331, 384, 446
HphI GGTGA 6 cut(s) 224, 262, 289, 322, 398, 610
Hpy188I TCNGA 1 cut(s) 359
Hpy188III TCNNGA 5 cut(s) 67, 203, 440, 446, 695
Hpy99I CGWCG 1 cut(s) 598
HpyAV CCTTC 5 cut(s) 116, 151, 214, 301, 722
HpyCH4III ACNGT 1 cut(s) 430
HpyF10VI GCNNNNNNNGC 1 cut(s) 38
Hsp92II CATG 4 cut(s) 155, 413, 491, 526
HspAI GCGC 1 cut(s) 87
Kpn2I TCCGGA 1 cut(s) 445
Kzo9I GATC 2 cut(s) 319, 442
LmnI GCTCC 2 cut(s) 243, 752
LpnPI CCDG 6 cut(s) 144, 175, 344, 397, 457, 459
Lsp1109I GCAGC 2 cut(s) 19, 641
MaeIII GTNAC 2 cut(s) 71, 430
MalI GATC 2 cut(s) 321, 444
MboI GATC 2 cut(s) 319, 442
MboII GAAGA 4 cut(s) 227, 545, 592, 674
MhlI GDGCHC 2 cut(s) 248, 342
MluCI AATT 1 cut(s) 253
MlyI GAGTC 2 cut(s) 336, 557
MmeI TCCRAC 2 cut(s) 75, 251
MroI TCCGGA 1 cut(s) 445
MseI TTAA 2 cut(s) 501, 707
MspI CCGG 5 cut(s) 131, 162, 331, 384, 446
MspR9I CCNGG 1 cut(s) 332
MvnI CGCG 2 cut(s) 89, 441
MwoI GCNNNNNNNGC 1 cut(s) 38
NciI CCSGG 1 cut(s) 332
NdeII GATC 2 cut(s) 319, 442
NlaIII CATG 4 cut(s) 155, 413, 491, 526
NmeAIII GCCGAG 2 cut(s) 51, 348
NmuCI GTSAC 1 cut(s) 430
NruI TCGCGA 1 cut(s) 441
NspI RCATGY 1 cut(s) 155
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 651
PfeI GAWTC 2 cut(s) 206, 449
PkrI GCNGC 4 cut(s) 7, 10, 34, 631
PleI GAGTC 2 cut(s) 335, 556
PpsI GAGTC 2 cut(s) 335, 556
PpuMI RGGWCCY 1 cut(s) 125
Psp124BI GAGCTC 2 cut(s) 248, 342
Psp5II RGGWCCY 1 cut(s) 125
PspPI GGNCC 2 cut(s) 125, 484
PspPPI RGGWCCY 1 cut(s) 125
PsrI GAACNNNNNNTAC 2 cut(s) 183, 215
RruI TCGCGA 1 cut(s) 441
SacI GAGCTC 2 cut(s) 248, 342
SaqAI TTAA 2 cut(s) 501, 707
SatI GCNGC 4 cut(s) 6, 9, 33, 630
Sau3AI GATC 2 cut(s) 319, 442
Sau96I GGNCC 2 cut(s) 125, 484
SchI GAGTC 2 cut(s) 336, 557
ScrFI CCNGG 1 cut(s) 332
SduI GDGCHC 2 cut(s) 248, 342
SinI GGWCC 2 cut(s) 125, 484
SmlI CTYRAG 2 cut(s) 341, 695
SmoI CTYRAG 2 cut(s) 341, 695
Sse9I AATT 1 cut(s) 253
SsiI CCGC 6 cut(s) 11, 89, 110, 197, 526, 568
SstI GAGCTC 2 cut(s) 248, 342
StyD4I CCNGG 1 cut(s) 330
TaaI ACNGT 1 cut(s) 430
TaqI TCGA 3 cut(s) 66, 425, 681
TasI AATT 1 cut(s) 253
TfiI GAWTC 2 cut(s) 206, 449
Tru1I TTAA 2 cut(s) 501, 707
Tru9I TTAA 2 cut(s) 501, 707
TseFI GTSAC 1 cut(s) 430
TseI GCWGC 4 cut(s) 5, 8, 32, 629
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 6 cut(s) 135, 156, 201, 343, 511, 675
VpaK11BI GGWCC 2 cut(s) 125, 484
XceI RCATGY 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.