Rroxscaffold_6G00391930

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0007563
Physical Location & Seq
Reverse (-)
10798272 .. 10809980
11709 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00391930.1

Sequence Viewer

Length: 786 bp
ATGGATAACAAGACTATTTTCCGCCGTAGCACAAATACAAGTATAAGAGCAAAGGTGTCTACAGCCACCATAGTCCCATCCCCTTGCTTTCCCTTGTACACATTCCCAAAACCTCCAGCACCCGGTATGTTCTTGGAGCTAAAATGGTTAAGTTTGAGAGGATCCGCCTTCAAACTGCTTAGTAGAGACGGGTTTGTGGTCCGAGAGTGGGCCGAGGGAGGGCCGAGGGCGGGACCGAGGGATGAGAGAGAGAGAGTGATTTTCAGCTGGAGGCTAGAGTGGGTGTTTTGCTCGATGGAATGGAGAGGGAAAGAGTTGAAAAAGCTTTTGAAGACTTCTCACGGTTATCTATCAACCACGGCAGGTCCTATAGCAGGCCTCTTCTTCATCTCCACACACAAGATTGCCTTACTGTCATCATCACCAAATCCAAATGGACAACTCATCAGAGTCCACTACAAGGTTGTGATACTACTCAATAAGATAAACAGAGTAAACCGGAGCGAGGATGTGAAGAAGCCATCACAAAACTGGAGATCATCGTACCGGCCATCATTCCTTGGAGCCACTGTGCTAAATCCTCCAGCTTCGATCGATCAAGATCAGGCCAACCCGGTCACGGCAAGCGGCTCGGCCATACCAACTCGAAACTCGACGCTAAATCCAAGTTTGGAGCGTCTGCTGCGGTGGGAGCCGTGTCATTCCGTGCGGGTCGGTGGAGACGAGATCGAGCGAGCTTCGACCGCCATGGTGCAGCTTGAGATGGAAGAAGCTGCTGCTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

261

Amino Acids

29.21

Weight (kDa)

10.0

Isoelectric Point (pI)

39.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 353
AccI GTMKAC 1 cut(s) 59
AciI CCGC 7 cut(s) 22, 165, 230, 627, 685, 709, 744
AclWI GGATC 2 cut(s) 156, 169
AcoI YGGCCR 2 cut(s) 548, 633
AfaI GTAC 2 cut(s) 98, 545
AfiI CCNNNNNNNGG 8 cut(s) 122, 208, 219, 230, 374, 460, 505, 619
AgsI TTSAA 3 cut(s) 172, 319, 331
AluBI AGCT 7 cut(s) 139, 267, 325, 587, 737, 757, 773
AluI AGCT 7 cut(s) 139, 267, 325, 587, 737, 757, 773
Alw26I GTCTC 2 cut(s) 180, 714
AlwI GGATC 2 cut(s) 156, 169
AoxI GGCC 6 cut(s) 210, 221, 376, 548, 606, 633
ApeKI GCWGC 4 cut(s) 682, 754, 773, 776
AspS9I GGNCC 5 cut(s) 199, 210, 221, 233, 365
AsuC2I CCSGG 2 cut(s) 123, 614
AsuHPI GGTGA 1 cut(s) 414
AvaII GGWCC 3 cut(s) 199, 233, 365
BamHI GGATCC 1 cut(s) 161
BbsI GAAGAC 1 cut(s) 338
BbvI GCAGC 4 cut(s) 669, 760, 763, 766
BccI CCATC 5 cut(s) 85, 289, 529, 559, 757
BceAI ACGGC 4 cut(s) 9, 375, 636, 679
BcgI CGANNNNNNTGC 2 cut(s) 612, 646
BcnI CCSGG 2 cut(s) 123, 614
BcoDI GTCTC 2 cut(s) 180, 714
BfaI CTAG 2 cut(s) 275, 780
BfmI CTRYAG 2 cut(s) 60, 369
BfuAI ACCTGC 1 cut(s) 353
BisI GCNGC 5 cut(s) 628, 683, 755, 774, 777
BlsI GCNGC 5 cut(s) 629, 684, 756, 775, 778
Bme1390I CCNGG 2 cut(s) 123, 614
Bme18I GGWCC 3 cut(s) 199, 233, 365
BmgT120I GGNCC 5 cut(s) 199, 210, 221, 233, 365
BmiI GGNNCC 4 cut(s) 163, 234, 565, 693
BmrFI CCNGG 2 cut(s) 123, 614
BpiI GAAGAC 1 cut(s) 338
BpmI CTGGAG 4 cut(s) 99, 289, 553, 567
BpuEI CTTGAG 1 cut(s) 779
BpuMI CCSGG 2 cut(s) 123, 614
Bsa29I ATCGAT 1 cut(s) 594
BsaBI GATNNNNATC 1 cut(s) 600
BsaJI CCNNGG 6 cut(s) 213, 224, 236, 357, 559, 747
BsaWI WCCGGW 1 cut(s) 498
BsaXI ACNNNNNCTCC 2 cut(s) 555, 585
Bsc4I CCNNNNNNNGG 8 cut(s) 122, 208, 219, 230, 374, 460, 505, 619
Bse118I RCCGGY 1 cut(s) 546
Bse1I ACTGG 1 cut(s) 536
Bse8I GATNNNNATC 1 cut(s) 600
BseCI ATCGAT 1 cut(s) 594
BseDI CCNNGG 6 cut(s) 213, 224, 236, 357, 559, 747
BseGI GGATG 3 cut(s) 77, 247, 514
BseJI GATNNNNATC 1 cut(s) 600
BseLI CCNNNNNNNGG 8 cut(s) 122, 208, 219, 230, 374, 460, 505, 619
BseNI ACTGG 1 cut(s) 536
BseXI GCAGC 4 cut(s) 669, 760, 763, 766
BsgI GTGCAG 1 cut(s) 773
Bsh1285I CGRYCG 2 cut(s) 594, 744
BshFI GGCC 6 cut(s) 212, 223, 378, 550, 608, 635
BshVI ATCGAT 1 cut(s) 594
BsiEI CGRYCG 2 cut(s) 594, 744
BsiSI CCGG 4 cut(s) 123, 499, 547, 614
BslFI GGGAC 2 cut(s) 59, 246
BslI CCNNNNNNNGG 8 cut(s) 122, 208, 219, 230, 374, 460, 505, 619
BsmAI GTCTC 2 cut(s) 180, 714
BsmBI CGTCTC 2 cut(s) 180, 714
BsmFI GGGAC 2 cut(s) 59, 246
BsnI GGCC 6 cut(s) 212, 223, 378, 550, 608, 635
Bsp1407I TGTACA 1 cut(s) 96
Bsp143I GATC 6 cut(s) 161, 536, 591, 595, 601, 726
Bsp19I CCATGG 1 cut(s) 747
BspACI CCGC 7 cut(s) 22, 165, 230, 627, 685, 709, 744
BspANI GGCC 6 cut(s) 212, 223, 378, 550, 608, 635
BspDI ATCGAT 1 cut(s) 594
BspLI GGNNCC 4 cut(s) 163, 234, 565, 693
BspMI ACCTGC 1 cut(s) 353
BspPI GGATC 2 cut(s) 156, 169
BsrFI RCCGGY 1 cut(s) 546
BsrGI TGTACA 1 cut(s) 96
BsrI ACTGG 1 cut(s) 536
BssAI RCCGGY 1 cut(s) 546
BssECI CCNNGG 6 cut(s) 213, 224, 236, 357, 559, 747
BssMI GATC 6 cut(s) 161, 536, 591, 595, 601, 726
BssT1I CCWWGG 2 cut(s) 559, 747
Bst4CI ACNGT 3 cut(s) 344, 414, 571
Bst6I CTCTTC 1 cut(s) 386
BstAUI TGTACA 1 cut(s) 96
BstC8I GCNNGC 3 cut(s) 376, 625, 735
BstDEI CTNAG 1 cut(s) 179
BstDSI CCRYGG 2 cut(s) 357, 747
BstENI CCTNNNNNAGG 1 cut(s) 372
BstF5I GGATG 3 cut(s) 77, 247, 514
BstKTI GATC 6 cut(s) 164, 539, 594, 598, 604, 729
BstMAI GTCTC 2 cut(s) 180, 714
BstMBI GATC 6 cut(s) 161, 536, 591, 595, 601, 726
BstMCI CGRYCG 2 cut(s) 594, 744
BstMWI GCNNNNNNNGC 3 cut(s) 682, 691, 743
BstSCI CCNGG 2 cut(s) 121, 612
BstSFI CTRYAG 2 cut(s) 60, 369
BstV1I GCAGC 4 cut(s) 669, 760, 763, 766
BstV2I GAAGAC 1 cut(s) 338
BstX2I RGATCY 1 cut(s) 161
BstYI RGATCY 1 cut(s) 161
Bsu15I ATCGAT 1 cut(s) 594
BsuRI GGCC 6 cut(s) 212, 223, 378, 550, 608, 635
BsuTUI ATCGAT 1 cut(s) 594
BtgI CCRYGG 2 cut(s) 357, 747
BtsCI GGATG 3 cut(s) 77, 247, 514
BtsIMutI CAGTG 1 cut(s) 567
BveI ACCTGC 1 cut(s) 353
Cac8I GCNNGC 3 cut(s) 376, 625, 735
Cfr10I RCCGGY 1 cut(s) 546
Cfr13I GGNCC 5 cut(s) 199, 210, 221, 233, 365
ClaI ATCGAT 1 cut(s) 594
CseI GACGC 2 cut(s) 664, 665
Csp6I GTAC 2 cut(s) 97, 544
CviAII CATG 1 cut(s) 748
CviQI GTAC 2 cut(s) 97, 544
DdeI CTNAG 1 cut(s) 179
DpnI GATC 6 cut(s) 163, 538, 593, 597, 603, 728
DpnII GATC 6 cut(s) 161, 536, 591, 595, 601, 726
EaeI YGGCCR 2 cut(s) 548, 633
Eam1104I CTCTTC 1 cut(s) 386
EarI CTCTTC 1 cut(s) 386
EciI GGCGGA 2 cut(s) 11, 154
Eco130I CCWWGG 2 cut(s) 559, 747
Eco147I AGGCCT 1 cut(s) 378
Eco47I GGWCC 3 cut(s) 199, 233, 365
EcoNI CCTNNNNNAGG 1 cut(s) 372
EcoO109I RGGNCCY 1 cut(s) 365
EcoT14I CCWWGG 2 cut(s) 559, 747
ErhI CCWWGG 2 cut(s) 559, 747
Esp3I CGTCTC 2 cut(s) 180, 714
FaeI CATG 1 cut(s) 751
FaiI YATR 6 cut(s) 44, 71, 128, 371, 638, 749
FalI AAGNNNNNCTT 2 cut(s) 392, 424
FaqI GGGAC 2 cut(s) 59, 246
FatI CATG 1 cut(s) 747
FauI CCCGC 2 cut(s) 223, 702
FblI GTMKAC 1 cut(s) 59
Fnu4HI GCNGC 5 cut(s) 628, 683, 755, 774, 777
FokI GGATG 3 cut(s) 64, 254, 521
Fsp4HI GCNGC 5 cut(s) 628, 683, 755, 774, 777
FspBI CTAG 2 cut(s) 275, 780
GluI GCNGC 5 cut(s) 628, 683, 755, 774, 777
GsuI CTGGAG 4 cut(s) 99, 289, 553, 567
HaeIII GGCC 6 cut(s) 212, 223, 378, 550, 608, 635
HapII CCGG 4 cut(s) 123, 499, 547, 614
HgaI GACGC 2 cut(s) 664, 665
Hin1II CATG 1 cut(s) 751
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 1 cut(s) 450
HpaII CCGG 4 cut(s) 123, 499, 547, 614
HphI GGTGA 1 cut(s) 414
Hpy166II GTNNAC 4 cut(s) 60, 99, 454, 496
Hpy188I TCNGA 2 cut(s) 203, 449
Hpy188III TCNNGA 1 cut(s) 599
Hpy8I GTNNAC 4 cut(s) 60, 99, 454, 496
Hpy99I CGWCG 1 cut(s) 658
HpyAV CCTTC 1 cut(s) 178
HpyCH4III ACNGT 3 cut(s) 344, 414, 571
HpyCH4V TGCA 1 cut(s) 754
HpyF10VI GCNNNNNNNGC 3 cut(s) 682, 691, 743
HpyF3I CTNAG 1 cut(s) 179
Hsp92II CATG 1 cut(s) 751
Kzo9I GATC 6 cut(s) 161, 536, 591, 595, 601, 726
LmnI GCTCC 5 cut(s) 136, 501, 563, 673, 691
Lsp1109I GCAGC 4 cut(s) 669, 760, 763, 766
MaeI CTAG 2 cut(s) 275, 780
MaeIII GTNAC 1 cut(s) 616
MalI GATC 6 cut(s) 163, 538, 593, 597, 603, 728
MboI GATC 6 cut(s) 161, 536, 591, 595, 601, 726
MboII GAAGA 5 cut(s) 343, 373, 376, 526, 779
MflI RGATCY 1 cut(s) 161
MlyI GAGTC 1 cut(s) 459
MseI TTAA 1 cut(s) 149
MspA1I CMGCKG 1 cut(s) 267
MspI CCGG 4 cut(s) 123, 499, 547, 614
MspR9I CCNGG 2 cut(s) 123, 614
MwoI GCNNNNNNNGC 3 cut(s) 682, 691, 743
NciI CCSGG 2 cut(s) 123, 614
NcoI CCATGG 1 cut(s) 747
NdeII GATC 6 cut(s) 161, 536, 591, 595, 601, 726
NlaIII CATG 1 cut(s) 751
NlaIV GGNNCC 4 cut(s) 163, 234, 565, 693
NmeAIII GCCGAG 3 cut(s) 238, 249, 611
NmuCI GTSAC 1 cut(s) 616
PceI AGGCCT 1 cut(s) 378
PcsI WCGNNNNNNNCGW 1 cut(s) 720
PkrI GCNGC 5 cut(s) 629, 684, 756, 775, 778
Ple19I CGATCG 1 cut(s) 594
PleI GAGTC 1 cut(s) 458
PpsI GAGTC 1 cut(s) 458
PpuMI RGGWCCY 1 cut(s) 365
Psp5II RGGWCCY 1 cut(s) 365
PspN4I GGNNCC 4 cut(s) 163, 234, 565, 693
PspPI GGNCC 5 cut(s) 199, 210, 221, 233, 365
PspPPI RGGWCCY 1 cut(s) 365
PsuI RGATCY 1 cut(s) 161
PvuI CGATCG 1 cut(s) 594
PvuII CAGCTG 1 cut(s) 267
RsaI GTAC 2 cut(s) 98, 545
RsaNI GTAC 2 cut(s) 97, 544
SaqAI TTAA 1 cut(s) 149
SatI GCNGC 5 cut(s) 628, 683, 755, 774, 777
Sau3AI GATC 6 cut(s) 161, 536, 591, 595, 601, 726
Sau96I GGNCC 5 cut(s) 199, 210, 221, 233, 365
SchI GAGTC 1 cut(s) 459
ScrFI CCNGG 2 cut(s) 123, 614
SfcI CTRYAG 2 cut(s) 60, 369
SinI GGWCC 3 cut(s) 199, 233, 365
SmlI CTYRAG 1 cut(s) 758
SmoI CTYRAG 1 cut(s) 758
SseBI AGGCCT 1 cut(s) 378
SsiI CCGC 7 cut(s) 22, 165, 230, 627, 685, 709, 744
SspMI CTAG 2 cut(s) 275, 780
StuI AGGCCT 1 cut(s) 378
StyD4I CCNGG 2 cut(s) 121, 612
StyI CCWWGG 2 cut(s) 559, 747
TaaI ACNGT 3 cut(s) 344, 414, 571
TaqI TCGA 7 cut(s) 293, 590, 594, 646, 653, 729, 740
TaqII GACCGA 1 cut(s) 250
TatI WGTACW 1 cut(s) 96
TauI GCSGC 1 cut(s) 630
Tru1I TTAA 1 cut(s) 149
Tru9I TTAA 1 cut(s) 149
TscAI CASTG 1 cut(s) 574
TseFI GTSAC 1 cut(s) 616
TseI GCWGC 4 cut(s) 682, 754, 773, 776
Tsp45I GTSAC 1 cut(s) 616
TspDTI ATGAA 1 cut(s) 376
TspGWI ACGGA 1 cut(s) 694
TspRI CASTG 1 cut(s) 574
VpaK11BI GGWCC 3 cut(s) 199, 233, 365
XagI CCTNNNNNAGG 1 cut(s) 372
XcmI CCANNNNNNNNNTGG 1 cut(s) 528
XmiI GTMKAC 1 cut(s) 59
XspI CTAG 2 cut(s) 275, 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.