Rroxscaffold_1G00072480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006927
Physical Location & Seq
Forward (+)
93518919 .. 93520243
1325 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00072480.1

Sequence Viewer

Length: 477 bp
ATGAGCACCGTCGTCAGCCTGCCGGAGAAGCGACAAGATCTCGGCCAGGGTTGGACCCCGGAGCAGCCGCCGGAAAAGCGTTCGAAGCTTGATGATCCACATCCGGGAGAGGTCGACGATACTGCAAAGGAATTGCAAATACGTAGCAAATCAGAAGAAGCGAAGTCCGCAGAAGAAGTTAAGTCCACGGGAGGTCGAGACATTAATCCTTATTGTGAGATCTGTTATGGCCATGTCGGTCACCAATCCTGGGAATGCCCTGACAAATCAAAGGGAATGGGAATGGGTTGTCGTCTTTGTTATGGCCCCTGTAAAAAGAAAGATGATGGTGATGGTGAGCACCGCTCAATTGAAATGGTCAAATATTGTATCGTTTGTGGGTCTATGGGCAGGCACTGGGGTGATGACTGCCCCTATTGGTCTGAAGCTAAAGTGGATAATTCCGAGATAAGTGATGAAGATTATGTTGGGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

17.69

Weight (kDa)

4.96

Isoelectric Point (pI)

49.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 345
AccI GTMKAC 1 cut(s) 114
AciI CCGC 3 cut(s) 68, 168, 343
AclWI GGATC 1 cut(s) 89
AcoI YGGCCR 2 cut(s) 43, 229
AcuI CTGAAG 1 cut(s) 444
AfiI CCNNNNNNNGG 2 cut(s) 104, 250
AgsI TTSAA 1 cut(s) 353
AjnI CCWGG 2 cut(s) 45, 248
AjuI GAANNNNNNNTTGG 1 cut(s) 450
AleI CACNNNNGTG 1 cut(s) 399
AluBI AGCT 2 cut(s) 88, 428
AluI AGCT 2 cut(s) 88, 428
Alw21I GWGCWC 2 cut(s) 8, 342
Alw26I GTCTC 1 cut(s) 192
AlwI GGATC 1 cut(s) 89
AlwNI CAGNNNCTG 1 cut(s) 396
AoxI GGCC 3 cut(s) 43, 229, 304
ApeKI GCWGC 1 cut(s) 64
AseI ATTAAT 1 cut(s) 204
AspS9I GGNCC 2 cut(s) 54, 305
AsuC2I CCSGG 2 cut(s) 59, 105
AsuHPI GGTGA 4 cut(s) 233, 341, 347, 413
AsuII TTCGAA 1 cut(s) 83
AvaII GGWCC 1 cut(s) 54
BalI TGGCCA 1 cut(s) 231
Bbv12I GWGCWC 2 cut(s) 8, 342
BbvI GCAGC 1 cut(s) 76
BccI CCATC 2 cut(s) 320, 326
BcgI CGANNNNNNTGC 2 cut(s) 104, 138
BciT130I CCWGG 2 cut(s) 47, 250
BcnI CCSGG 2 cut(s) 59, 105
BcoDI GTCTC 1 cut(s) 192
BfaI CTAG 1 cut(s) 475
BglII AGATCT 2 cut(s) 37, 219
BisI GCNGC 2 cut(s) 65, 68
BlsI GCNGC 2 cut(s) 66, 69
Bme1390I CCNGG 4 cut(s) 47, 59, 105, 250
Bme18I GGWCC 1 cut(s) 54
BmgT120I GGNCC 2 cut(s) 54, 305
BmiI GGNNCC 2 cut(s) 56, 307
BmrFI CCNGG 4 cut(s) 47, 59, 105, 250
BmrI ACTGGG 1 cut(s) 406
BmuI ACTGGG 1 cut(s) 406
BplI GAGNNNNNCTC 2 cut(s) 329, 361
Bpu14I TTCGAA 1 cut(s) 83
BpuMI CCSGG 2 cut(s) 59, 105
BsaAI YACGTR 1 cut(s) 143
BsaBI GATNNNNATC 1 cut(s) 99
BsaJI CCNNGG 4 cut(s) 46, 57, 186, 249
Bsc4I CCNNNNNNNGG 2 cut(s) 104, 250
Bse1I ACTGG 1 cut(s) 401
Bse8I GATNNNNATC 1 cut(s) 99
BseBI CCWGG 2 cut(s) 47, 250
BseDI CCNNGG 4 cut(s) 46, 57, 186, 249
BseGI GGATG 1 cut(s) 100
BseJI GATNNNNATC 1 cut(s) 99
BseLI CCNNNNNNNGG 2 cut(s) 104, 250
BseNI ACTGG 1 cut(s) 401
BseXI GCAGC 1 cut(s) 76
BshFI GGCC 3 cut(s) 45, 231, 306
BsiHKAI GWGCWC 2 cut(s) 8, 342
BsiSI CCGG 4 cut(s) 23, 59, 71, 104
BslI CCNNNNNNNGG 2 cut(s) 104, 250
BsmAI GTCTC 1 cut(s) 192
BsmI GAATGC 1 cut(s) 260
BsnI GGCC 3 cut(s) 45, 231, 306
Bsp119I TTCGAA 1 cut(s) 83
Bsp1286I GDGCHC 2 cut(s) 8, 342
Bsp143I GATC 3 cut(s) 37, 94, 219
BspACI CCGC 3 cut(s) 68, 168, 343
BspANI GGCC 3 cut(s) 45, 231, 306
BspLI GGNNCC 2 cut(s) 56, 307
BspPI GGATC 1 cut(s) 89
BspT104I TTCGAA 1 cut(s) 83
BsrBI CCGCTC 1 cut(s) 345
BsrI ACTGG 1 cut(s) 401
BssECI CCNNGG 4 cut(s) 46, 57, 186, 249
BssMI GATC 3 cut(s) 37, 94, 219
Bst2UI CCWGG 2 cut(s) 47, 250
Bst4CI ACNGT 1 cut(s) 10
BstBAI YACGTR 1 cut(s) 143
BstBI TTCGAA 1 cut(s) 83
BstC8I GCNNGC 2 cut(s) 20, 392
BstDSI CCRYGG 1 cut(s) 186
BstEII GGTNACC 1 cut(s) 239
BstF5I GGATG 1 cut(s) 100
BstKTI GATC 3 cut(s) 40, 97, 222
BstMAI GTCTC 1 cut(s) 192
BstMBI GATC 3 cut(s) 37, 94, 219
BstMWI GCNNNNNNNGC 4 cut(s) 28, 76, 85, 167
BstNI CCWGG 2 cut(s) 47, 250
BstPI GGTNACC 1 cut(s) 239
BstSCI CCNGG 4 cut(s) 45, 57, 103, 248
BstSNI TACGTA 1 cut(s) 143
BstV1I GCAGC 1 cut(s) 76
BstX2I RGATCY 2 cut(s) 37, 219
BstYI RGATCY 2 cut(s) 37, 219
BsuRI GGCC 3 cut(s) 45, 231, 306
BtgI CCRYGG 1 cut(s) 186
BtsCI GGATG 1 cut(s) 100
BtsIMutI CAGTG 1 cut(s) 394
Cac8I GCNNGC 2 cut(s) 20, 392
CaiI CAGNNNCTG 1 cut(s) 396
Cfr13I GGNCC 2 cut(s) 54, 305
CviAII CATG 1 cut(s) 233
CviJI RGCY 7 cut(s) 18, 45, 67, 88, 231, 306, 428
CviKI_1 RGCY 7 cut(s) 18, 45, 67, 88, 231, 306, 428
DpnI GATC 3 cut(s) 39, 96, 221
DpnII GATC 3 cut(s) 37, 94, 219
EaeI YGGCCR 2 cut(s) 43, 229
Eco105I TACGTA 1 cut(s) 143
Eco47I GGWCC 1 cut(s) 54
Eco57I CTGAAG 1 cut(s) 444
Eco91I GGTNACC 1 cut(s) 239
EcoO65I GGTNACC 1 cut(s) 239
EcoRII CCWGG 2 cut(s) 45, 248
FaeI CATG 1 cut(s) 236
FaiI YATR 5 cut(s) 228, 234, 303, 386, 465
FatI CATG 1 cut(s) 232
FblI GTMKAC 1 cut(s) 114
Fnu4HI GCNGC 2 cut(s) 65, 68
FokI GGATG 1 cut(s) 87
Fsp4HI GCNGC 2 cut(s) 65, 68
FspBI CTAG 1 cut(s) 475
GluI GCNGC 2 cut(s) 65, 68
HaeIII GGCC 3 cut(s) 45, 231, 306
HapII CCGG 4 cut(s) 23, 59, 71, 104
Hin1II CATG 1 cut(s) 236
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HindIII AAGCTT 1 cut(s) 86
HpaII CCGG 4 cut(s) 23, 59, 71, 104
HphI GGTGA 4 cut(s) 233, 341, 347, 413
Hpy166II GTNNAC 2 cut(s) 115, 186
Hpy188I TCNGA 3 cut(s) 154, 424, 445
Hpy188III TCNNGA 1 cut(s) 197
Hpy8I GTNNAC 2 cut(s) 115, 186
Hpy99I CGWCG 2 cut(s) 14, 119
HpyCH4III ACNGT 1 cut(s) 10
HpyCH4IV ACGT 1 cut(s) 142
HpyCH4V TGCA 2 cut(s) 125, 136
HpyF10VI GCNNNNNNNGC 4 cut(s) 28, 76, 85, 167
HpySE526I ACGT 1 cut(s) 142
Hsp92II CATG 1 cut(s) 236
Kzo9I GATC 3 cut(s) 37, 94, 219
LmnI GCTCC 1 cut(s) 61
Lsp1109I GCAGC 1 cut(s) 76
MaeI CTAG 1 cut(s) 475
MaeII ACGT 1 cut(s) 142
MaeIII GTNAC 1 cut(s) 239
MalI GATC 3 cut(s) 39, 96, 221
MbiI CCGCTC 1 cut(s) 345
MboI GATC 3 cut(s) 37, 94, 219
MboII GAAGA 3 cut(s) 167, 185, 470
MfeI CAATTG 1 cut(s) 348
MflI RGATCY 2 cut(s) 37, 219
MhlI GDGCHC 2 cut(s) 8, 342
MlsI TGGCCA 1 cut(s) 231
MluCI AATT 3 cut(s) 131, 348, 439
MluNI TGGCCA 1 cut(s) 231
MmeI TCCRAC 1 cut(s) 32
MnlI CCTC 2 cut(s) 103, 185
Mox20I TGGCCA 1 cut(s) 231
MscI TGGCCA 1 cut(s) 231
MseI TTAA 2 cut(s) 180, 204
MslI CAYNNNNRTG 1 cut(s) 399
Msp20I TGGCCA 1 cut(s) 231
MspI CCGG 4 cut(s) 23, 59, 71, 104
MspR9I CCNGG 4 cut(s) 47, 59, 105, 250
MunI CAATTG 1 cut(s) 348
Mva1269I GAATGC 1 cut(s) 260
MvaI CCWGG 2 cut(s) 47, 250
MwoI GCNNNNNNNGC 4 cut(s) 28, 76, 85, 167
NciI CCSGG 2 cut(s) 59, 105
NdeII GATC 3 cut(s) 37, 94, 219
NlaIII CATG 1 cut(s) 236
NlaIV GGNNCC 2 cut(s) 56, 307
NmeAIII GCCGAG 1 cut(s) 21
NmuCI GTSAC 1 cut(s) 239
NspV TTCGAA 1 cut(s) 83
OliI CACNNNNGTG 1 cut(s) 399
PctI GAATGC 1 cut(s) 260
PfoI TCCNGGA 1 cut(s) 103
PkrI GCNGC 2 cut(s) 66, 69
Ppu21I YACGTR 1 cut(s) 143
PshBI ATTAAT 1 cut(s) 204
Psp6I CCWGG 2 cut(s) 45, 248
PspEI GGTNACC 1 cut(s) 239
PspGI CCWGG 2 cut(s) 45, 248
PspN4I GGNNCC 2 cut(s) 56, 307
PspPI GGNCC 2 cut(s) 54, 305
PstNI CAGNNNCTG 1 cut(s) 396
PsuI RGATCY 2 cut(s) 37, 219
RseI CAYNNNNRTG 1 cut(s) 399
SalI GTCGAC 1 cut(s) 113
SaqAI TTAA 2 cut(s) 180, 204
SatI GCNGC 2 cut(s) 65, 68
Sau3AI GATC 3 cut(s) 37, 94, 219
Sau96I GGNCC 2 cut(s) 54, 305
ScrFI CCNGG 4 cut(s) 47, 59, 105, 250
SduI GDGCHC 2 cut(s) 8, 342
SetI ASST 5 cut(s) 90, 114, 145, 196, 430
SfuI TTCGAA 1 cut(s) 83
SinI GGWCC 1 cut(s) 54
SmiMI CAYNNNNRTG 1 cut(s) 399
SnaBI TACGTA 1 cut(s) 143
Sse9I AATT 3 cut(s) 131, 348, 439
SsiI CCGC 3 cut(s) 68, 168, 343
SspI AATATT 1 cut(s) 365
SspMI CTAG 1 cut(s) 475
StyD4I CCNGG 4 cut(s) 45, 57, 103, 248
TaaI ACNGT 1 cut(s) 10
TaiI ACGT 1 cut(s) 145
TaqI TCGA 3 cut(s) 83, 114, 196
TaqII GACCGA 1 cut(s) 227
TasI AATT 3 cut(s) 131, 348, 439
TauI GCSGC 1 cut(s) 70
Tru1I TTAA 2 cut(s) 180, 204
Tru9I TTAA 2 cut(s) 180, 204
TscAI CASTG 1 cut(s) 401
TseFI GTSAC 1 cut(s) 239
TseI GCWGC 1 cut(s) 64
Tsp45I GTSAC 1 cut(s) 239
TspDTI ATGAA 1 cut(s) 471
TspRI CASTG 1 cut(s) 401
VpaK11BI GGWCC 1 cut(s) 54
VspI ATTAAT 1 cut(s) 204
XmiI GTMKAC 1 cut(s) 114
XspI CTAG 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.