FvH4_4g03960

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006927
Physical Location & Seq
Forward (+)
3429338 .. 3431232
1895 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g03960.t1

Sequence Viewer

Length: 477 bp
ATGAGCAGCATCGTCACTTTGCCGGAGAAGCGAAATGGTCTCGGCCAGGATTGGTTCCCGAAGCAACCGCAAGAGAAGCATGCCAAGCTTGATGATCCATATCCAGGAGAGGTCTACGATACTGCAATGGAATTGAAAGACAATAGCAATTCAGAAGAATCAAAATCTGGGGGAGGCTCTTCAACTGAACTGGACGATGATATTGAGGATTACTGTGAGATCTGTCGTGGCCATGTCGGTCACGAATGCTGGGATTGCCCTTACAAATCAAAGGGAATAGGTTGGGGTTGCATTGAATGTTACGGCCCCTGTAAAAAGGAAGACGGCGATCCTAGTGAGCATTGGTCTATTGAAATGGTCAAACAATGTGTCGTATGTGGGAATATGGGTAATCACTGGGCTGATGACTGCCCCTATCGGTCTGATGCTCAAGTGGATGACGCCGGGACAAGCGACCATGACGGTCTTGGCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.53

Weight (kDa)

4.41

Isoelectric Point (pI)

50.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 114
AciI CCGC 1 cut(s) 68
AclWI GGATC 2 cut(s) 89, 323
AcoI YGGCCR 2 cut(s) 43, 229
AcyI GRCGYC 1 cut(s) 441
AfiI CCNNNNNNNGG 1 cut(s) 104
AgsI TTSAA 4 cut(s) 136, 183, 296, 353
AjnI CCWGG 2 cut(s) 45, 103
AluBI AGCT 1 cut(s) 88
AluI AGCT 1 cut(s) 88
Alw26I GTCTC 1 cut(s) 44
AlwI GGATC 2 cut(s) 89, 323
AoxI GGCC 3 cut(s) 43, 229, 304
ApeKI GCWGC 1 cut(s) 6
ArsI GACNNNNNNTTYG 2 cut(s) 354, 386
AspS9I GGNCC 1 cut(s) 305
AsuC2I CCSGG 1 cut(s) 445
BalI TGGCCA 1 cut(s) 231
BbsI GAAGAC 1 cut(s) 327
BbvI GCAGC 1 cut(s) 18
BceAI ACGGC 2 cut(s) 319, 340
BciT130I CCWGG 2 cut(s) 47, 105
BcnI CCSGG 1 cut(s) 445
BcoDI GTCTC 1 cut(s) 44
BfaI CTAG 1 cut(s) 333
BglII AGATCT 1 cut(s) 219
BisI GCNGC 1 cut(s) 7
BlsI GCNGC 1 cut(s) 8
Bme1390I CCNGG 3 cut(s) 47, 105, 445
BmgT120I GGNCC 1 cut(s) 305
BmiI GGNNCC 2 cut(s) 56, 307
BmrFI CCNGG 3 cut(s) 47, 105, 445
BmrI ACTGGG 1 cut(s) 406
BmsI GCATC 2 cut(s) 18, 415
BmuI ACTGGG 1 cut(s) 406
BpiI GAAGAC 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 414
BpuMI CCSGG 1 cut(s) 445
BsaBI GATNNNNATC 1 cut(s) 99
BsaHI GRCGYC 1 cut(s) 441
BsaI GGTCTC 1 cut(s) 44
Bsc4I CCNNNNNNNGG 1 cut(s) 104
Bse1I ACTGG 2 cut(s) 195, 401
Bse3DI GCAATG 1 cut(s) 132
Bse8I GATNNNNATC 1 cut(s) 99
BseBI CCWGG 2 cut(s) 47, 105
BseGI GGATG 1 cut(s) 442
BseJI GATNNNNATC 1 cut(s) 99
BseLI CCNNNNNNNGG 1 cut(s) 104
BseMI GCAATG 1 cut(s) 132
BseNI ACTGG 2 cut(s) 195, 401
BseXI GCAGC 1 cut(s) 18
BseYI CCCAGC 1 cut(s) 249
BshFI GGCC 3 cut(s) 45, 231, 306
BsiSI CCGG 2 cut(s) 23, 444
BslFI GGGAC 1 cut(s) 460
BslI CCNNNNNNNGG 1 cut(s) 104
BsmAI GTCTC 1 cut(s) 44
BsmFI GGGAC 1 cut(s) 460
BsmI GAATGC 1 cut(s) 251
BsnI GGCC 3 cut(s) 45, 231, 306
Bso31I GGTCTC 1 cut(s) 44
Bsp143I GATC 3 cut(s) 94, 219, 328
BspACI CCGC 1 cut(s) 68
BspANI GGCC 3 cut(s) 45, 231, 306
BspLI GGNNCC 2 cut(s) 56, 307
BspPI GGATC 2 cut(s) 89, 323
BspQI GCTCTTC 1 cut(s) 184
BspTNI GGTCTC 1 cut(s) 44
BsrDI GCAATG 1 cut(s) 132
BsrI ACTGG 2 cut(s) 195, 401
BssMI GATC 3 cut(s) 94, 219, 328
BssNI GRCGYC 1 cut(s) 441
Bst2UI CCWGG 2 cut(s) 47, 105
Bst4CI ACNGT 2 cut(s) 215, 464
Bst6I CTCTTC 1 cut(s) 184
BstACI GRCGYC 1 cut(s) 441
BstC8I GCNNGC 1 cut(s) 81
BstF5I GGATG 1 cut(s) 442
BstKTI GATC 3 cut(s) 97, 222, 331
BstMAI GTCTC 1 cut(s) 44
BstMBI GATC 3 cut(s) 94, 219, 328
BstMWI GCNNNNNNNGC 4 cut(s) 28, 76, 85, 255
BstNI CCWGG 2 cut(s) 47, 105
BstNSI RCATGY 1 cut(s) 83
BstSCI CCNGG 3 cut(s) 45, 103, 443
BstV1I GCAGC 1 cut(s) 18
BstV2I GAAGAC 1 cut(s) 327
BstX2I RGATCY 1 cut(s) 219
BstYI RGATCY 1 cut(s) 219
BsuRI GGCC 3 cut(s) 45, 231, 306
BtsCI GGATG 1 cut(s) 442
BtsIMutI CAGTG 1 cut(s) 394
Cac8I GCNNGC 1 cut(s) 81
Cfr13I GGNCC 1 cut(s) 305
CseI GACGC 1 cut(s) 449
CviAII CATG 3 cut(s) 80, 233, 458
CviJI RGCY 7 cut(s) 45, 88, 177, 231, 306, 401, 471
CviKI_1 RGCY 7 cut(s) 45, 88, 177, 231, 306, 401, 471
DpnI GATC 3 cut(s) 96, 221, 330
DpnII GATC 3 cut(s) 94, 219, 328
EaeI YGGCCR 2 cut(s) 43, 229
Eam1104I CTCTTC 1 cut(s) 184
EarI CTCTTC 1 cut(s) 184
Eco31I GGTCTC 1 cut(s) 44
EcoRII CCWGG 2 cut(s) 45, 103
FaeI CATG 3 cut(s) 83, 236, 461
FaiI YATR 6 cut(s) 81, 100, 234, 376, 386, 459
FaqI GGGAC 1 cut(s) 460
FatI CATG 3 cut(s) 79, 232, 457
FblI GTMKAC 1 cut(s) 114
Fnu4HI GCNGC 1 cut(s) 7
FokI GGATG 1 cut(s) 449
Fsp4HI GCNGC 1 cut(s) 7
FspBI CTAG 1 cut(s) 333
GluI GCNGC 1 cut(s) 7
GsaI CCCAGC 1 cut(s) 253
HaeIII GGCC 3 cut(s) 45, 231, 306
HapII CCGG 2 cut(s) 23, 444
HgaI GACGC 1 cut(s) 449
Hin1I GRCGYC 1 cut(s) 441
Hin1II CATG 3 cut(s) 83, 236, 461
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 1 cut(s) 158
HpaII CCGG 2 cut(s) 23, 444
Hpy166II GTNNAC 1 cut(s) 115
Hpy188I TCNGA 2 cut(s) 154, 424
Hpy188III TCNNGA 2 cut(s) 58, 242
Hpy8I GTNNAC 1 cut(s) 115
HpyCH4III ACNGT 2 cut(s) 215, 464
HpyCH4V TGCA 2 cut(s) 125, 291
HpyF10VI GCNNNNNNNGC 4 cut(s) 28, 76, 85, 255
Hsp92I GRCGYC 1 cut(s) 441
Hsp92II CATG 3 cut(s) 83, 236, 461
Kzo9I GATC 3 cut(s) 94, 219, 328
LguI GCTCTTC 1 cut(s) 184
Lsp1109I GCAGC 1 cut(s) 18
LweI GCATC 2 cut(s) 18, 415
MaeI CTAG 1 cut(s) 333
MaeIII GTNAC 3 cut(s) 13, 239, 299
MalI GATC 3 cut(s) 96, 221, 330
MboI GATC 3 cut(s) 94, 219, 328
MboII GAAGA 3 cut(s) 167, 171, 332
MflI RGATCY 1 cut(s) 219
MlsI TGGCCA 1 cut(s) 231
MluCI AATT 2 cut(s) 131, 148
MluNI TGGCCA 1 cut(s) 231
MnlI CCTC 3 cut(s) 103, 167, 199
Mox20I TGGCCA 1 cut(s) 231
MscI TGGCCA 1 cut(s) 231
Msp20I TGGCCA 1 cut(s) 231
MspI CCGG 2 cut(s) 23, 444
MspR9I CCNGG 3 cut(s) 47, 105, 445
Mva1269I GAATGC 1 cut(s) 251
MvaI CCWGG 2 cut(s) 47, 105
MwoI GCNNNNNNNGC 4 cut(s) 28, 76, 85, 255
NciI CCSGG 1 cut(s) 445
NdeII GATC 3 cut(s) 94, 219, 328
NlaIII CATG 3 cut(s) 83, 236, 461
NlaIV GGNNCC 2 cut(s) 56, 307
NmeAIII GCCGAG 1 cut(s) 21
NmuCI GTSAC 2 cut(s) 13, 239
NspI RCATGY 1 cut(s) 83
PaeI GCATGC 1 cut(s) 83
PciSI GCTCTTC 1 cut(s) 184
PctI GAATGC 1 cut(s) 251
PfeI GAWTC 1 cut(s) 158
PfoI TCCNGGA 1 cut(s) 103
PkrI GCNGC 1 cut(s) 8
Psp6I CCWGG 2 cut(s) 45, 103
PspFI CCCAGC 1 cut(s) 249
PspGI CCWGG 2 cut(s) 45, 103
PspN4I GGNNCC 2 cut(s) 56, 307
PspPI GGNCC 1 cut(s) 305
PsuI RGATCY 1 cut(s) 219
SapI GCTCTTC 1 cut(s) 184
SatI GCNGC 1 cut(s) 7
Sau3AI GATC 3 cut(s) 94, 219, 328
Sau96I GGNCC 1 cut(s) 305
ScrFI CCNGG 3 cut(s) 47, 105, 445
SetI ASST 3 cut(s) 90, 114, 283
SfaNI GCATC 2 cut(s) 18, 415
SmlI CTYRAG 1 cut(s) 429
SmoI CTYRAG 1 cut(s) 429
SphI GCATGC 1 cut(s) 83
Sse9I AATT 2 cut(s) 131, 148
SsiI CCGC 1 cut(s) 68
SspMI CTAG 1 cut(s) 333
StyD4I CCNGG 3 cut(s) 45, 103, 443
TaaI ACNGT 2 cut(s) 215, 464
TaqII GACCGA 2 cut(s) 227, 408
TasI AATT 2 cut(s) 131, 148
TfiI GAWTC 1 cut(s) 158
TscAI CASTG 1 cut(s) 401
TseFI GTSAC 2 cut(s) 13, 239
TseI GCWGC 1 cut(s) 6
Tsp45I GTSAC 2 cut(s) 13, 239
TspRI CASTG 1 cut(s) 401
XceI RCATGY 1 cut(s) 83
XcmI CCANNNNNNNNNTGG 1 cut(s) 464
XmiI GTMKAC 1 cut(s) 114
XspI CTAG 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.