Rw5G044860

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0007892
Physical Location & Seq
Reverse (-)
78575587 .. 78578452
2866 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G044860.1

Sequence Viewer

Length: 777 bp
ATGTCATTGTTGCTGCAGCAACGGGCTTGGAAGCCGAAAGCAAAGAACACCGCTTTCGAAGTGGAGCCAAGACGATCCACCCGTCAGCGAAACCCAATTCAAACATATGTTGATGATGTTGACATAGGCCTTCCAACTAGGAAGAGGTTGAGGTCAAGTTCATCTTCATTTGGAAGCTACCTTGCAAGGCCTTTGGAGGAATGTCGAATGGCTTCTTATGAAGAAAGGAGTGCTGCTTTCAAAGCAGCAGAGCGGCTTCAAGAGAGTCTGCAATCTGAGAATCCATCTTTTGTCAAATCGATGCTTCGGTCTCATGTCTATAGCTGTTTTTGGTTGGGTCTTCCTTCTAATTTCTGTGAGACTCATCTCCCAAAATCATCGGACTCTGAACTGATACTAGAGGATGAAGATGGAAATGAATATGATGCTAAATACATTGCCAAGAGGACTGGTCTTAGTGGTGGTTGGAGAGGTTTTGCATTGGAACACAAGTTGGACGATGGTGATGCACTTGTGTTCGAATTGGTTGAACCCACAAGGTTTAAGATCTACATAGTTAAGGTGACAAATCAAGAACATTGCAAGAAGGACAAAGTGAAGAGTGCCGGAAAGCCATCAAAGGCAGCCAAGAAGCCTGACCAAGACTCAAACAAAAAACAAAGGTCAAAGAAAGCTACCACTGCTGAAATAATCGACATGAAAACACCCCCAGAGCTTCTACCAGAGAGCCCAAAAAGTGAAGCTGTACTACCATATAGACTAAGGAAGAGAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

258

Amino Acids

29.4

Weight (kDa)

9.4

Isoelectric Point (pI)

61.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 97 - 185 4.1e-19 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 253
AciI CCGC 2 cut(s) 51, 253
AclWI GGATC 1 cut(s) 69
AfaI GTAC 1 cut(s) 747
AgsI TTSAA 4 cut(s) 101, 241, 260, 530
AluBI AGCT 5 cut(s) 177, 324, 674, 715, 743
AluI AGCT 5 cut(s) 177, 324, 674, 715, 743
Alw26I GTCTC 2 cut(s) 315, 353
AlwI GGATC 1 cut(s) 69
AoxI GGCC 2 cut(s) 127, 188
ApeKI GCWGC 5 cut(s) 13, 16, 233, 245, 623
Asp700I GAANNNNTTC 1 cut(s) 211
AsuHPI GGTGA 2 cut(s) 515, 574
AsuII TTCGAA 2 cut(s) 57, 519
BanII GRGCYC 1 cut(s) 731
BarI GAAGNNNNNNTAC 2 cut(s) 732, 764
BbsI GAAGAC 1 cut(s) 332
BbvI GCAGC 4 cut(s) 28, 220, 257, 635
BccI CCATC 4 cut(s) 292, 404, 494, 622
BcgI CGANNNNNNTGC 2 cut(s) 488, 522
BcoDI GTCTC 2 cut(s) 315, 353
BfaI CTAG 2 cut(s) 138, 398
BfmI CTRYAG 2 cut(s) 14, 319
BglII AGATCT 1 cut(s) 546
BisI GCNGC 6 cut(s) 14, 17, 234, 246, 254, 624
BlsI GCNGC 6 cut(s) 15, 18, 235, 247, 255, 625
BmiI GGNNCC 1 cut(s) 66
BmsI GCATC 3 cut(s) 291, 415, 496
BpiI GAAGAC 1 cut(s) 332
Bpu14I TTCGAA 2 cut(s) 57, 519
Bsa29I ATCGAT 1 cut(s) 299
BsaI GGTCTC 1 cut(s) 315
Bse1I ACTGG 1 cut(s) 454
Bse3DI GCAATG 2 cut(s) 435, 577
BseCI ATCGAT 1 cut(s) 299
BseGI GGATG 1 cut(s) 409
BseMI GCAATG 2 cut(s) 435, 577
BseMII CTCAG 1 cut(s) 267
BseNI ACTGG 1 cut(s) 454
BseXI GCAGC 4 cut(s) 28, 220, 257, 635
BshFI GGCC 2 cut(s) 129, 190
BshVI ATCGAT 1 cut(s) 299
BsiSI CCGG 1 cut(s) 606
BsmAI GTCTC 2 cut(s) 315, 353
BsnI GGCC 2 cut(s) 129, 190
Bso31I GGTCTC 1 cut(s) 315
Bsp119I TTCGAA 2 cut(s) 57, 519
Bsp1286I GDGCHC 1 cut(s) 731
Bsp143I GATC 2 cut(s) 74, 546
BspACI CCGC 2 cut(s) 51, 253
BspANI GGCC 2 cut(s) 129, 190
BspCNI CTCAG 1 cut(s) 268
BspDI ATCGAT 1 cut(s) 299
BspLI GGNNCC 1 cut(s) 66
BspMAI CTGCAG 1 cut(s) 18
BspPI GGATC 1 cut(s) 69
BspT104I TTCGAA 2 cut(s) 57, 519
BspTNI GGTCTC 1 cut(s) 315
BsrBI CCGCTC 1 cut(s) 253
BsrDI GCAATG 2 cut(s) 435, 577
BsrI ACTGG 1 cut(s) 454
BssMI GATC 2 cut(s) 74, 546
Bst6I CTCTTC 3 cut(s) 137, 593, 761
BstBI TTCGAA 2 cut(s) 57, 519
BstDEI CTNAG 3 cut(s) 276, 455, 761
BstF5I GGATG 1 cut(s) 409
BstKTI GATC 2 cut(s) 77, 549
BstMAI GTCTC 2 cut(s) 315, 353
BstMBI GATC 2 cut(s) 74, 546
BstMWI GCNNNNNNNGC 2 cut(s) 242, 680
BstSFI CTRYAG 2 cut(s) 14, 319
BstV1I GCAGC 4 cut(s) 28, 220, 257, 635
BstV2I GAAGAC 1 cut(s) 332
BstX2I RGATCY 1 cut(s) 546
BstYI RGATCY 1 cut(s) 546
Bsu15I ATCGAT 1 cut(s) 299
BsuRI GGCC 2 cut(s) 129, 190
BsuTUI ATCGAT 1 cut(s) 299
BtsCI GGATG 1 cut(s) 409
BtsI GCAGTG 1 cut(s) 678
BtsIMutI CAGTG 1 cut(s) 678
ClaI ATCGAT 1 cut(s) 299
Csp6I GTAC 1 cut(s) 746
CviAII CATG 2 cut(s) 314, 697
CviQI GTAC 1 cut(s) 746
DdeI CTNAG 3 cut(s) 276, 455, 761
DpnI GATC 2 cut(s) 76, 548
DpnII GATC 2 cut(s) 74, 546
Eam1104I CTCTTC 3 cut(s) 137, 593, 761
EarI CTCTTC 3 cut(s) 137, 593, 761
Eco147I AGGCCT 2 cut(s) 129, 190
Eco24I GRGCYC 1 cut(s) 731
Eco31I GGTCTC 1 cut(s) 315
EcoT38I GRGCYC 1 cut(s) 731
FaeI CATG 2 cut(s) 317, 700
FalI AAGNNNNNCTT 2 cut(s) 148, 180
FatI CATG 2 cut(s) 313, 696
FauNDI CATATG 1 cut(s) 106
Fnu4HI GCNGC 6 cut(s) 14, 17, 234, 246, 254, 624
FokI GGATG 1 cut(s) 416
FriOI GRGCYC 1 cut(s) 731
Fsp4HI GCNGC 6 cut(s) 14, 17, 234, 246, 254, 624
FspBI CTAG 2 cut(s) 138, 398
GluI GCNGC 6 cut(s) 14, 17, 234, 246, 254, 624
HaeIII GGCC 2 cut(s) 129, 190
HapII CCGG 1 cut(s) 606
Hin1II CATG 2 cut(s) 317, 700
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HinfI GANTC 5 cut(s) 265, 280, 361, 383, 644
HpaII CCGG 1 cut(s) 606
HphI GGTGA 2 cut(s) 515, 574
Hpy166II GTNNAC 1 cut(s) 121
Hpy188I TCNGA 3 cut(s) 277, 382, 388
Hpy188III TCNNGA 2 cut(s) 260, 572
Hpy8I GTNNAC 1 cut(s) 121
HpyAV CCTTC 3 cut(s) 140, 354, 580
HpyCH4V TGCA 6 cut(s) 16, 185, 271, 479, 509, 582
HpyF10VI GCNNNNNNNGC 2 cut(s) 242, 680
HpyF3I CTNAG 3 cut(s) 276, 455, 761
Hsp92II CATG 2 cut(s) 317, 700
Kzo9I GATC 2 cut(s) 74, 546
LmnI GCTCC 1 cut(s) 64
LpnPI CCDG 5 cut(s) 435, 619, 648, 723, 735
Lsp1109I GCAGC 4 cut(s) 28, 220, 257, 635
LweI GCATC 3 cut(s) 291, 415, 496
MaeI CTAG 2 cut(s) 138, 398
MaeIII GTNAC 1 cut(s) 562
MalI GATC 2 cut(s) 76, 548
MbiI CCGCTC 1 cut(s) 253
MboI GATC 2 cut(s) 74, 546
MboII GAAGA 6 cut(s) 154, 156, 233, 332, 419, 610
MflI RGATCY 1 cut(s) 546
MhlI GDGCHC 1 cut(s) 731
MluCI AATT 3 cut(s) 96, 349, 521
MlyI GAGTC 4 cut(s) 274, 355, 377, 638
MmeI TCCRAC 3 cut(s) 158, 446, 474
MnlI CCTC 6 cut(s) 138, 144, 190, 394, 438, 464
MroXI GAANNNNTTC 1 cut(s) 211
MseI TTAA 2 cut(s) 543, 558
MspI CCGG 1 cut(s) 606
MwoI GCNNNNNNNGC 2 cut(s) 242, 680
NdeI CATATG 1 cut(s) 106
NdeII GATC 2 cut(s) 74, 546
NlaIII CATG 2 cut(s) 317, 700
NlaIV GGNNCC 1 cut(s) 66
NmuCI GTSAC 1 cut(s) 562
NspV TTCGAA 2 cut(s) 57, 519
PceI AGGCCT 2 cut(s) 129, 190
PcsI WCGNNNNNNNCGW 1 cut(s) 79
PdmI GAANNNNTTC 1 cut(s) 211
PfeI GAWTC 1 cut(s) 280
PkrI GCNGC 6 cut(s) 15, 18, 235, 247, 255, 625
PleI GAGTC 4 cut(s) 273, 355, 377, 638
PpsI GAGTC 4 cut(s) 273, 355, 377, 638
PspN4I GGNNCC 1 cut(s) 66
PstI CTGCAG 1 cut(s) 18
PsuI RGATCY 1 cut(s) 546
RsaI GTAC 1 cut(s) 747
RsaNI GTAC 1 cut(s) 746
SaqAI TTAA 2 cut(s) 543, 558
SatI GCNGC 6 cut(s) 14, 17, 234, 246, 254, 624
Sau3AI GATC 2 cut(s) 74, 546
SchI GAGTC 4 cut(s) 274, 355, 377, 638
SduI GDGCHC 1 cut(s) 731
SfaNI GCATC 3 cut(s) 291, 415, 496
SfcI CTRYAG 2 cut(s) 14, 319
SfuI TTCGAA 2 cut(s) 57, 519
Sse9I AATT 3 cut(s) 96, 349, 521
SseBI AGGCCT 2 cut(s) 129, 190
SsiI CCGC 2 cut(s) 51, 253
SspMI CTAG 2 cut(s) 138, 398
StuI AGGCCT 2 cut(s) 129, 190
TaqI TCGA 5 cut(s) 57, 205, 299, 519, 693
TaqII GACCGA 1 cut(s) 297
TasI AATT 3 cut(s) 96, 349, 521
TatI WGTACW 1 cut(s) 745
TauI GCSGC 1 cut(s) 256
TfiI GAWTC 1 cut(s) 280
Tru1I TTAA 2 cut(s) 543, 558
Tru9I TTAA 2 cut(s) 543, 558
TscAI CASTG 1 cut(s) 685
TseFI GTSAC 1 cut(s) 562
TseI GCWGC 5 cut(s) 13, 16, 233, 245, 623
Tsp45I GTSAC 1 cut(s) 562
TspDTI ATGAA 6 cut(s) 150, 156, 234, 420, 432, 713
TspRI CASTG 1 cut(s) 685
XmnI GAANNNNTTC 1 cut(s) 211
XspI CTAG 2 cut(s) 138, 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.