Rh5BG503900

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0007892
Physical Location & Seq
Reverse (-)
80053619 .. 80058439
4821 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG503900.1

Sequence Viewer

Length: 666 bp
ATGCAGGTTGACATAGGCCTTCCAACTAGGAAGAGGTTGAGGTCAAGTTCATCTTCATTTGGAAGCTACCTTGCAAGGCCTTTGGAGGAATGCCGAATGGCTTCTTATGAAGAAAGGAGTGATGCTTTCAAAGCAGCAGAGCGGCTTCAAGAGAGTCTGCAATCTGAGAATCCATCTTTTGTCAAATCGATGCTTCGGTCTCATGTCTATAGCTGTTTTTGGTTGGGTCTTCCTTCTAATTTCTGTGAGACTCATCTCCCAAAATCATCGGACTCTGAACTGATACTAGAGGATGAAGATGGAAATGTATATGATGCTAAATATATTGCCAAGAGGACTGGTCTTAGTGGTGGTTGGAGAGGTTTTGCATTGGAACACAAGTTGGACGATGGTGATGCACTTGTGTTCGAATTGGTTGAACCCACAAGGTTTAAGATCTACATAGTTAAGGTGACAAATCAAGAACATTGCAAGAAGGACAAAGTGAAGAGTGCTGGAAAGCCATCAAAGGCAGCCAAGAAGCCTGACCAAGACTCTAACAAAAAACAAAGGTCAAAGAAAGCTACCACTGCTGAAATAATCGACATGAAAACACCCCCAGAGCTTCTACCAGAGAGCCCAAAAAGTGAAGCCGTACTACCATATAGACTCAGGAAGAGAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

221

Amino Acids

25.02

Weight (kDa)

9.13

Isoelectric Point (pI)

59.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 60 - 148 4.2e-19 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 142
AciI CCGC 1 cut(s) 142
AfaI GTAC 1 cut(s) 636
AgsI TTSAA 3 cut(s) 130, 149, 419
AluBI AGCT 4 cut(s) 66, 213, 563, 604
AluI AGCT 4 cut(s) 66, 213, 563, 604
Alw26I GTCTC 2 cut(s) 204, 242
AoxI GGCC 2 cut(s) 16, 77
ApeKI GCWGC 2 cut(s) 134, 512
Asp700I GAANNNNTTC 1 cut(s) 100
AsuHPI GGTGA 2 cut(s) 404, 463
AsuII TTCGAA 1 cut(s) 408
BanII GRGCYC 1 cut(s) 620
BarI GAAGNNNNNNTAC 2 cut(s) 621, 653
BbsI GAAGAC 1 cut(s) 221
BbvI GCAGC 2 cut(s) 146, 524
BccI CCATC 4 cut(s) 181, 293, 383, 511
BceAI ACGGC 1 cut(s) 617
BcgI CGANNNNNNTGC 2 cut(s) 377, 411
BcoDI GTCTC 2 cut(s) 204, 242
BfaI CTAG 2 cut(s) 27, 287
BfmI CTRYAG 1 cut(s) 208
BglII AGATCT 1 cut(s) 435
BisI GCNGC 3 cut(s) 135, 143, 513
BlsI GCNGC 3 cut(s) 136, 144, 514
BmsI GCATC 4 cut(s) 112, 180, 304, 385
BpiI GAAGAC 1 cut(s) 221
Bpu14I TTCGAA 1 cut(s) 408
Bsa29I ATCGAT 1 cut(s) 188
BsaI GGTCTC 1 cut(s) 204
Bse1I ACTGG 1 cut(s) 343
Bse3DI GCAATG 1 cut(s) 466
BseCI ATCGAT 1 cut(s) 188
BseGI GGATG 1 cut(s) 298
BseMI GCAATG 1 cut(s) 466
BseMII CTCAG 2 cut(s) 156, 664
BseNI ACTGG 1 cut(s) 343
BseXI GCAGC 2 cut(s) 146, 524
BshFI GGCC 2 cut(s) 18, 79
BshVI ATCGAT 1 cut(s) 188
BsmAI GTCTC 2 cut(s) 204, 242
BsmI GAATGC 1 cut(s) 95
BsnI GGCC 2 cut(s) 18, 79
Bso31I GGTCTC 1 cut(s) 204
Bsp119I TTCGAA 1 cut(s) 408
Bsp1286I GDGCHC 1 cut(s) 620
Bsp143I GATC 1 cut(s) 435
BspACI CCGC 1 cut(s) 142
BspANI GGCC 2 cut(s) 18, 79
BspCNI CTCAG 2 cut(s) 157, 663
BspDI ATCGAT 1 cut(s) 188
BspT104I TTCGAA 1 cut(s) 408
BspTNI GGTCTC 1 cut(s) 204
BsrBI CCGCTC 1 cut(s) 142
BsrDI GCAATG 1 cut(s) 466
BsrI ACTGG 1 cut(s) 343
BssMI GATC 1 cut(s) 435
Bst6I CTCTTC 3 cut(s) 26, 482, 650
BstBI TTCGAA 1 cut(s) 408
BstDEI CTNAG 3 cut(s) 165, 344, 650
BstF5I GGATG 1 cut(s) 298
BstKTI GATC 1 cut(s) 438
BstMAI GTCTC 2 cut(s) 204, 242
BstMBI GATC 1 cut(s) 435
BstMWI GCNNNNNNNGC 2 cut(s) 131, 569
BstSFI CTRYAG 1 cut(s) 208
BstV1I GCAGC 2 cut(s) 146, 524
BstV2I GAAGAC 1 cut(s) 221
BstX2I RGATCY 1 cut(s) 435
BstYI RGATCY 1 cut(s) 435
Bsu15I ATCGAT 1 cut(s) 188
BsuRI GGCC 2 cut(s) 18, 79
BsuTUI ATCGAT 1 cut(s) 188
BtsCI GGATG 1 cut(s) 298
BtsI GCAGTG 1 cut(s) 567
BtsIMutI CAGTG 1 cut(s) 567
ClaI ATCGAT 1 cut(s) 188
Csp6I GTAC 1 cut(s) 635
CviAII CATG 2 cut(s) 203, 586
CviQI GTAC 1 cut(s) 635
DdeI CTNAG 3 cut(s) 165, 344, 650
DpnI GATC 1 cut(s) 437
DpnII GATC 1 cut(s) 435
Eam1104I CTCTTC 3 cut(s) 26, 482, 650
EarI CTCTTC 3 cut(s) 26, 482, 650
Eco147I AGGCCT 2 cut(s) 18, 79
Eco24I GRGCYC 1 cut(s) 620
Eco31I GGTCTC 1 cut(s) 204
EcoT38I GRGCYC 1 cut(s) 620
FaeI CATG 2 cut(s) 206, 589
FalI AAGNNNNNCTT 2 cut(s) 37, 69
FatI CATG 2 cut(s) 202, 585
Fnu4HI GCNGC 3 cut(s) 135, 143, 513
FokI GGATG 1 cut(s) 305
FriOI GRGCYC 1 cut(s) 620
Fsp4HI GCNGC 3 cut(s) 135, 143, 513
FspBI CTAG 2 cut(s) 27, 287
GluI GCNGC 3 cut(s) 135, 143, 513
HaeIII GGCC 2 cut(s) 18, 79
Hin1II CATG 2 cut(s) 206, 589
HincII GTYRAC 1 cut(s) 10
HindII GTYRAC 1 cut(s) 10
HinfI GANTC 6 cut(s) 154, 169, 250, 272, 533, 648
HphI GGTGA 2 cut(s) 404, 463
Hpy166II GTNNAC 1 cut(s) 10
Hpy188I TCNGA 3 cut(s) 166, 271, 277
Hpy188III TCNNGA 3 cut(s) 149, 461, 652
Hpy8I GTNNAC 1 cut(s) 10
HpyAV CCTTC 3 cut(s) 29, 243, 469
HpyCH4V TGCA 6 cut(s) 4, 74, 160, 368, 398, 471
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 569
HpyF3I CTNAG 3 cut(s) 165, 344, 650
Hsp92II CATG 2 cut(s) 206, 589
Kzo9I GATC 1 cut(s) 435
LpnPI CCDG 6 cut(s) 324, 480, 537, 612, 624, 637
Lsp1109I GCAGC 2 cut(s) 146, 524
LweI GCATC 4 cut(s) 112, 180, 304, 385
MaeI CTAG 2 cut(s) 27, 287
MaeIII GTNAC 1 cut(s) 451
MalI GATC 1 cut(s) 437
MbiI CCGCTC 1 cut(s) 142
MboI GATC 1 cut(s) 435
MboII GAAGA 6 cut(s) 43, 45, 122, 221, 308, 499
MflI RGATCY 1 cut(s) 435
MhlI GDGCHC 1 cut(s) 620
MluCI AATT 2 cut(s) 238, 410
MlyI GAGTC 5 cut(s) 163, 244, 266, 527, 642
MmeI TCCRAC 3 cut(s) 47, 335, 363
MnlI CCTC 6 cut(s) 27, 33, 79, 283, 327, 353
MroXI GAANNNNTTC 1 cut(s) 100
MseI TTAA 2 cut(s) 432, 447
Mva1269I GAATGC 1 cut(s) 95
MwoI GCNNNNNNNGC 2 cut(s) 131, 569
NdeII GATC 1 cut(s) 435
NlaIII CATG 2 cut(s) 206, 589
NmuCI GTSAC 1 cut(s) 451
NspV TTCGAA 1 cut(s) 408
PceI AGGCCT 2 cut(s) 18, 79
PctI GAATGC 1 cut(s) 95
PdmI GAANNNNTTC 1 cut(s) 100
PfeI GAWTC 1 cut(s) 169
PkrI GCNGC 3 cut(s) 136, 144, 514
PleI GAGTC 5 cut(s) 162, 244, 266, 527, 642
PpsI GAGTC 5 cut(s) 162, 244, 266, 527, 642
PsuI RGATCY 1 cut(s) 435
RsaI GTAC 1 cut(s) 636
RsaNI GTAC 1 cut(s) 635
SaqAI TTAA 2 cut(s) 432, 447
SatI GCNGC 3 cut(s) 135, 143, 513
Sau3AI GATC 1 cut(s) 435
SchI GAGTC 5 cut(s) 163, 244, 266, 527, 642
SduI GDGCHC 1 cut(s) 620
SfaNI GCATC 4 cut(s) 112, 180, 304, 385
SfcI CTRYAG 1 cut(s) 208
SfuI TTCGAA 1 cut(s) 408
Sse9I AATT 2 cut(s) 238, 410
SseBI AGGCCT 2 cut(s) 18, 79
SsiI CCGC 1 cut(s) 142
SspMI CTAG 2 cut(s) 27, 287
StuI AGGCCT 2 cut(s) 18, 79
TaqI TCGA 3 cut(s) 188, 408, 582
TaqII GACCGA 1 cut(s) 186
TasI AATT 2 cut(s) 238, 410
TauI GCSGC 1 cut(s) 145
TfiI GAWTC 1 cut(s) 169
Tru1I TTAA 2 cut(s) 432, 447
Tru9I TTAA 2 cut(s) 432, 447
TscAI CASTG 1 cut(s) 574
TseFI GTSAC 1 cut(s) 451
TseI GCWGC 2 cut(s) 134, 512
Tsp45I GTSAC 1 cut(s) 451
TspDTI ATGAA 5 cut(s) 39, 45, 123, 309, 602
TspRI CASTG 1 cut(s) 574
XmnI GAANNNNTTC 1 cut(s) 100
XspI CTAG 2 cut(s) 27, 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.