Rh6BG387800

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0007892
Physical Location & Seq
Forward (+)
61569961 .. 61583289
13329 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG387800.1

Sequence Viewer

Length: 1038 bp
ATGCGTAGTTACGTGAGAAACAAGGGTGAAAGAAGAGGGGAGAGCTCAGAGTGCTCAGACATACTGTTGAAGCTGCAGATGAATATAGGTACCGCCGTCCCGGCCGGTACAACTAGCAAGGATGGAGTTGTCTGGGCCGACCTCAGGACCATCAAGAAGGCTACCAGTAGTGTGTTCCCGGCGAAGAGAAGGTTGGTGAAGACCGTGGTTTTTGCTACCGTTTTGCCCTTCTTCGCCTCTATTTTATGTTCCTCTGGGGAAGTTGGTGGAGGAAACCAGAAATCCCAACTGCCAAGAAATTCAAGCAGATGTGAAAAGTTAGAGGCAGCGCGTATCGCCTGTAACAAGTACATGGCCAAGTTCGCCGGCAAGGACGCGTTCCATCTACGCGTTAGGGTTCACCCTTTCCATGTGCTCAGGATTAACAAGATGCTTTCCTGCGCCGGAGCCGATAGGCTTCAGACTGCTACCTTGCAAGGCCTTTGGAGGAATGCCGAATGGCTTCTTATGAAGAAAGGAGTGCCGCTTTCAAAGCTGCAGAGCGGCTTCAAGAGAGTTTGCAATCTGAAAATCCATCTTTTGTCAAATAGATGGTTTGGTCTCATGTCTATAGCTGTTTTTGGACTAGTATTATTGCTTGATTTGCTAATCTACTTGTTTACTAATTCCTGTTCCGATTACAAGGGTCTTCCTTCTAATTTCTGTGAGACTCATCTCCCACAATCACCGGACTCTGAACTGATACTAGAGGATGAAGATGGAAATGAATATGATGCTAAATACATTGCCAAGAGGACTAGTCTTAGTGGTGGTTGGAGATGTTTTGCATTGGAACACAAGTTGGACGATGGTGATGCACTTGTGTTTGAATTGGTTGAACCCACAAGGTTTAAGATCTACATAGTTAAGGTGACAAATCAAGAACATTGCAAGAAGGACAAAGTGAAGAGTGCTGGAAAGCCATCAAAGGCAGCCAAGAAGCCTGACCAAGACACTAACAAAAAACAAAGGTCAAAGAAAGCTAGCTACCACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

345

Amino Acids

38.71

Weight (kDa)

9.68

Isoelectric Point (pI)

53.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L16 PF00252 105 - 155 1.1e-07 Ribosomal protein L16p/L10e
B3 PF02362 228 - 301 5.7e-14 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 89
AccB1I GGYRCC 1 cut(s) 89
AccBSI CCGCTC 1 cut(s) 543
AccII CGCG 3 cut(s) 331, 377, 390
AciI CCGC 3 cut(s) 93, 524, 543
AcoI YGGCCR 2 cut(s) 102, 354
AcsI RAATTY 1 cut(s) 298
AcuI CTGAAG 1 cut(s) 443
AfaI GTAC 3 cut(s) 91, 109, 350
AfiI CCNNNNNNNGG 1 cut(s) 144
AflIII ACRYGT 2 cut(s) 375, 388
AgsI TTSAA 6 cut(s) 70, 303, 531, 550, 869, 878
AhlI ACTAGT 2 cut(s) 625, 797
AjuI GAANNNNNNNTTGG 2 cut(s) 176, 208
AluBI AGCT 6 cut(s) 45, 73, 535, 614, 1022, 1026
AluI AGCT 6 cut(s) 45, 73, 535, 614, 1022, 1026
Alw21I GWGCWC 3 cut(s) 47, 56, 417
Alw26I GTCTC 2 cut(s) 605, 701
AoxI GGCC 4 cut(s) 102, 135, 354, 478
ApeKI GCWGC 4 cut(s) 73, 326, 535, 971
ApoI RAATTY 1 cut(s) 298
Asp700I GAANNNNTTC 1 cut(s) 501
Asp718I GGTACC 1 cut(s) 89
AspLEI GCGC 2 cut(s) 331, 443
AspS9I GGNCC 2 cut(s) 135, 147
AsuC2I CCSGG 2 cut(s) 101, 179
AsuHPI GGTGA 6 cut(s) 38, 208, 392, 717, 863, 922
AsuNHI GCTAGC 1 cut(s) 1022
AvaII GGWCC 1 cut(s) 147
AxyI CCTNAGG 1 cut(s) 143
BaeI ACNNNNGTAYC 2 cut(s) 81, 114
BalI TGGCCA 1 cut(s) 356
BanI GGYRCC 1 cut(s) 89
BanII GRGCYC 1 cut(s) 47
BbsI GAAGAC 2 cut(s) 206, 680
Bbv12I GWGCWC 3 cut(s) 47, 56, 417
BbvI GCAGC 4 cut(s) 60, 338, 522, 983
BccI CCATC 8 cut(s) 116, 158, 390, 582, 585, 752, 842, 970
BceAI ACGGC 1 cut(s) 80
BcgI CGANNNNNNTGC 2 cut(s) 836, 870
BcnI CCSGG 2 cut(s) 101, 179
BcoDI GTCTC 2 cut(s) 605, 701
BcuI ACTAGT 2 cut(s) 625, 797
BfaI CTAG 5 cut(s) 114, 626, 746, 798, 1023
BfmI CTRYAG 3 cut(s) 74, 536, 609
BglI GCCNNNNNGGC 1 cut(s) 101
BglII AGATCT 1 cut(s) 894
BisI GCNGC 6 cut(s) 74, 327, 524, 536, 544, 972
BlsI GCNGC 6 cut(s) 75, 328, 525, 537, 545, 973
Bme1390I CCNGG 2 cut(s) 101, 179
Bme18I GGWCC 1 cut(s) 147
BmgT120I GGNCC 2 cut(s) 135, 147
BmiI GGNNCC 2 cut(s) 91, 448
BmrFI CCNGG 2 cut(s) 101, 179
BmsI GCATC 3 cut(s) 420, 763, 844
BmtI GCTAGC 1 cut(s) 1026
BpiI GAAGAC 2 cut(s) 206, 680
Bpu10I CCTNAGC 1 cut(s) 416
BpuMI CCSGG 2 cut(s) 101, 179
BsaAI YACGTR 1 cut(s) 13
BsaI GGTCTC 1 cut(s) 605
BsaJI CCNNGG 1 cut(s) 204
BsaWI WCCGGW 1 cut(s) 727
Bsc4I CCNNNNNNNGG 1 cut(s) 144
Bse118I RCCGGY 2 cut(s) 104, 365
Bse1I ACTGG 1 cut(s) 165
Bse21I CCTNAGG 1 cut(s) 143
Bse3DI GCAATG 2 cut(s) 783, 925
BseDI CCNNGG 1 cut(s) 204
BseGI GGATG 2 cut(s) 127, 757
BseLI CCNNNNNNNGG 1 cut(s) 144
BseMI GCAATG 2 cut(s) 783, 925
BseMII CTCAG 4 cut(s) 60, 69, 157, 430
BseNI ACTGG 1 cut(s) 165
BseX3I CGGCCG 1 cut(s) 102
BseXI GCAGC 4 cut(s) 60, 338, 522, 983
Bsh1236I CGCG 3 cut(s) 331, 377, 390
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 4 cut(s) 104, 137, 356, 480
BshNI GGYRCC 1 cut(s) 89
BsiEI CGRYCG 1 cut(s) 105
BsiHKAI GWGCWC 3 cut(s) 47, 56, 417
BsiSI CCGG 6 cut(s) 101, 105, 179, 366, 444, 728
BslFI GGGAC 1 cut(s) 83
BslI CCNNNNNNNGG 1 cut(s) 144
BsmAI GTCTC 2 cut(s) 605, 701
BsmFI GGGAC 1 cut(s) 83
BsmI GAATGC 1 cut(s) 496
BsnI GGCC 4 cut(s) 104, 137, 356, 480
Bso31I GGTCTC 1 cut(s) 605
Bsp1286I GDGCHC 3 cut(s) 47, 56, 417
Bsp143I GATC 1 cut(s) 894
BspACI CCGC 3 cut(s) 93, 524, 543
BspANI GGCC 4 cut(s) 104, 137, 356, 480
BspCNI CTCAG 4 cut(s) 59, 68, 156, 429
BspFNI CGCG 3 cut(s) 331, 377, 390
BspLI GGNNCC 2 cut(s) 91, 448
BspMAI CTGCAG 2 cut(s) 78, 540
BspOI GCTAGC 1 cut(s) 1026
BspT107I GGYRCC 1 cut(s) 89
BspTNI GGTCTC 1 cut(s) 605
BsrBI CCGCTC 1 cut(s) 543
BsrDI GCAATG 2 cut(s) 783, 925
BsrFI RCCGGY 2 cut(s) 104, 365
BsrI ACTGG 1 cut(s) 165
BssAI RCCGGY 2 cut(s) 104, 365
BssECI CCNNGG 1 cut(s) 204
BssMI GATC 1 cut(s) 894
Bst4CI ACNGT 3 cut(s) 66, 205, 220
Bst6I CTCTTC 3 cut(s) 28, 179, 941
BstBAI YACGTR 1 cut(s) 13
BstC8I GCNNGC 2 cut(s) 367, 1024
BstDEI CTNAG 5 cut(s) 46, 55, 143, 416, 803
BstDSI CCRYGG 1 cut(s) 204
BstF5I GGATG 2 cut(s) 127, 757
BstFNI CGCG 3 cut(s) 331, 377, 390
BstHHI GCGC 2 cut(s) 331, 443
BstKTI GATC 1 cut(s) 897
BstMAI GTCTC 2 cut(s) 605, 701
BstMBI GATC 1 cut(s) 894
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 6 cut(s) 51, 101, 335, 362, 532, 643
BstSCI CCNGG 2 cut(s) 99, 177
BstSFI CTRYAG 3 cut(s) 74, 536, 609
BstUI CGCG 3 cut(s) 331, 377, 390
BstV1I GCAGC 4 cut(s) 60, 338, 522, 983
BstV2I GAAGAC 2 cut(s) 206, 680
BstX2I RGATCY 1 cut(s) 894
BstYI RGATCY 1 cut(s) 894
BstZI CGGCCG 1 cut(s) 102
Bsu36I CCTNAGG 1 cut(s) 143
BsuRI GGCC 4 cut(s) 104, 137, 356, 480
BtgI CCRYGG 1 cut(s) 204
BtsCI GGATG 2 cut(s) 127, 757
Cac8I GCNNGC 2 cut(s) 367, 1024
CfoI GCGC 2 cut(s) 331, 443
Cfr10I RCCGGY 2 cut(s) 104, 365
Cfr13I GGNCC 2 cut(s) 135, 147
CseI GACGC 1 cut(s) 383
Csp6I GTAC 3 cut(s) 90, 108, 349
CviAII CATG 3 cut(s) 352, 410, 604
CviQI GTAC 3 cut(s) 90, 108, 349
DdeI CTNAG 5 cut(s) 46, 55, 143, 416, 803
DpnI GATC 1 cut(s) 896
DpnII GATC 1 cut(s) 894
EaeI YGGCCR 2 cut(s) 102, 354
EagI CGGCCG 1 cut(s) 102
Eam1104I CTCTTC 3 cut(s) 28, 179, 941
EarI CTCTTC 3 cut(s) 28, 179, 941
Ecl136II GAGCTC 1 cut(s) 45
EclXI CGGCCG 1 cut(s) 102
Eco147I AGGCCT 1 cut(s) 480
Eco24I GRGCYC 1 cut(s) 47
Eco31I GGTCTC 1 cut(s) 605
Eco47I GGWCC 1 cut(s) 147
Eco52I CGGCCG 1 cut(s) 102
Eco53kI GAGCTC 1 cut(s) 45
Eco57I CTGAAG 1 cut(s) 443
Eco81I CCTNAGG 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 45
EcoT38I GRGCYC 1 cut(s) 47
FaeI CATG 3 cut(s) 355, 413, 607
FaqI GGGAC 1 cut(s) 83
FatI CATG 3 cut(s) 351, 409, 603
Fnu4HI GCNGC 6 cut(s) 74, 327, 524, 536, 544, 972
FokI GGATG 2 cut(s) 134, 764
FriOI GRGCYC 1 cut(s) 47
Fsp4HI GCNGC 6 cut(s) 74, 327, 524, 536, 544, 972
FspBI CTAG 5 cut(s) 114, 626, 746, 798, 1023
GlaI GCGC 2 cut(s) 330, 442
GluI GCNGC 6 cut(s) 74, 327, 524, 536, 544, 972
HaeIII GGCC 4 cut(s) 104, 137, 356, 480
HapII CCGG 6 cut(s) 101, 105, 179, 366, 444, 728
HgaI GACGC 1 cut(s) 383
HhaI GCGC 2 cut(s) 331, 443
Hin1II CATG 3 cut(s) 355, 413, 607
Hin6I GCGC 2 cut(s) 329, 441
HinP1I GCGC 2 cut(s) 329, 441
HinfI GANTC 2 cut(s) 709, 731
HpaII CCGG 6 cut(s) 101, 105, 179, 366, 444, 728
HphI GGTGA 6 cut(s) 38, 208, 392, 717, 863, 922
Hpy166II GTNNAC 2 cut(s) 400, 660
Hpy188I TCNGA 6 cut(s) 49, 58, 462, 567, 676, 736
Hpy188III TCNNGA 5 cut(s) 145, 154, 418, 550, 920
Hpy8I GTNNAC 2 cut(s) 400, 660
HpyAV CCTTC 5 cut(s) 151, 183, 238, 702, 928
HpyCH4III ACNGT 3 cut(s) 66, 205, 220
HpyCH4IV ACGT 1 cut(s) 12
HpyCH4V TGCA 7 cut(s) 76, 475, 538, 561, 827, 857, 930
HpyF10VI GCNNNNNNNGC 6 cut(s) 51, 101, 335, 362, 532, 643
HpyF3I CTNAG 5 cut(s) 46, 55, 143, 416, 803
HpySE526I ACGT 1 cut(s) 12
Hsp92II CATG 3 cut(s) 355, 413, 607
HspAI GCGC 2 cut(s) 329, 441
KpnI GGTACC 1 cut(s) 93
KroI GCCGGC 1 cut(s) 365
KroNI GCCGGC 1 cut(s) 367
Kzo9I GATC 1 cut(s) 894
LmnI GCTCC 1 cut(s) 446
Lsp1109I GCAGC 4 cut(s) 60, 338, 522, 983
LweI GCATC 3 cut(s) 420, 763, 844
MaeI CTAG 5 cut(s) 114, 626, 746, 798, 1023
MaeII ACGT 1 cut(s) 12
MaeIII GTNAC 3 cut(s) 8, 341, 910
MalI GATC 1 cut(s) 896
MbiI CCGCTC 1 cut(s) 543
MboI GATC 1 cut(s) 894
MboII GAAGA 8 cut(s) 45, 196, 211, 223, 523, 680, 767, 958
MflI RGATCY 1 cut(s) 894
MhlI GDGCHC 3 cut(s) 47, 56, 417
MlsI TGGCCA 1 cut(s) 356
MluCI AATT 4 cut(s) 298, 664, 697, 869
MluI ACGCGT 2 cut(s) 375, 388
MluNI TGGCCA 1 cut(s) 356
MlyI GAGTC 2 cut(s) 703, 725
MmeI TCCRAC 2 cut(s) 794, 822
MnlI CCTC 9 cut(s) 29, 152, 247, 262, 263, 316, 480, 742, 786
Mox20I TGGCCA 1 cut(s) 356
MroNI GCCGGC 1 cut(s) 365
MroXI GAANNNNTTC 1 cut(s) 501
MscI TGGCCA 1 cut(s) 356
MseI TTAA 3 cut(s) 423, 891, 906
Msp20I TGGCCA 1 cut(s) 356
MspI CCGG 6 cut(s) 101, 105, 179, 366, 444, 728
MspR9I CCNGG 2 cut(s) 101, 179
Mva1269I GAATGC 1 cut(s) 496
MvnI CGCG 3 cut(s) 331, 377, 390
MwoI GCNNNNNNNGC 6 cut(s) 51, 101, 335, 362, 532, 643
NaeI GCCGGC 1 cut(s) 367
NciI CCSGG 2 cut(s) 101, 179
NdeII GATC 1 cut(s) 894
NgoMIV GCCGGC 1 cut(s) 365
NheI GCTAGC 1 cut(s) 1022
NlaIII CATG 3 cut(s) 355, 413, 607
NlaIV GGNNCC 2 cut(s) 91, 448
NmuCI GTSAC 1 cut(s) 910
PceI AGGCCT 1 cut(s) 480
PctI GAATGC 1 cut(s) 496
PdiI GCCGGC 1 cut(s) 367
PdmI GAANNNNTTC 1 cut(s) 501
PkrI GCNGC 6 cut(s) 75, 328, 525, 537, 545, 973
PleI GAGTC 2 cut(s) 703, 725
PpsI GAGTC 2 cut(s) 703, 725
Ppu21I YACGTR 1 cut(s) 13
Psp124BI GAGCTC 1 cut(s) 47
PspN4I GGNNCC 2 cut(s) 91, 448
PspPI GGNCC 2 cut(s) 135, 147
PstI CTGCAG 2 cut(s) 78, 540
PsuI RGATCY 1 cut(s) 894
RsaI GTAC 3 cut(s) 91, 109, 350
RsaNI GTAC 3 cut(s) 90, 108, 349
SacI GAGCTC 1 cut(s) 47
SaqAI TTAA 3 cut(s) 423, 891, 906
SatI GCNGC 6 cut(s) 74, 327, 524, 536, 544, 972
Sau3AI GATC 1 cut(s) 894
Sau96I GGNCC 2 cut(s) 135, 147
SchI GAGTC 2 cut(s) 703, 725
ScrFI CCNGG 2 cut(s) 101, 179
SduI GDGCHC 3 cut(s) 47, 56, 417
SfaNI GCATC 3 cut(s) 420, 763, 844
SfcI CTRYAG 3 cut(s) 74, 536, 609
SinI GGWCC 1 cut(s) 147
SpeI ACTAGT 2 cut(s) 625, 797
Sse9I AATT 4 cut(s) 298, 664, 697, 869
SseBI AGGCCT 1 cut(s) 480
SsiI CCGC 3 cut(s) 93, 524, 543
SspMI CTAG 5 cut(s) 114, 626, 746, 798, 1023
SstI GAGCTC 1 cut(s) 47
StuI AGGCCT 1 cut(s) 480
StyD4I CCNGG 2 cut(s) 99, 177
TaaI ACNGT 3 cut(s) 66, 205, 220
TaiI ACGT 1 cut(s) 15
TasI AATT 4 cut(s) 298, 664, 697, 869
TatI WGTACW 1 cut(s) 348
TauI GCSGC 2 cut(s) 526, 546
Tru1I TTAA 3 cut(s) 423, 891, 906
Tru9I TTAA 3 cut(s) 423, 891, 906
TseFI GTSAC 1 cut(s) 910
TseI GCWGC 4 cut(s) 73, 326, 535, 971
Tsp45I GTSAC 1 cut(s) 910
TspDTI ATGAA 4 cut(s) 95, 524, 768, 780
VpaK11BI GGWCC 1 cut(s) 147
XapI RAATTY 1 cut(s) 298
XmnI GAANNNNTTC 1 cut(s) 501
XspI CTAG 5 cut(s) 114, 626, 746, 798, 1023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.