MD03G1047900.v1.1

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007892
Physical Location & Seq
Forward (+)
3844202 .. 3847064
2863 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1047900.v1.1.491

Sequence Viewer

Length: 1596 bp
ATGGTTGCCATCGCCGCCACCACTTACGAAGAAGCTCGCAACCAGCGTCTCGAAGAAAACAAAAGGAAATTTCAGGATTTGGGAATCTCAAGCATTTCAAAGACATTGACCCACTTGACTGCTTCTCCGAACAAGACCCAGCATCGTGTCTCGAAACCTAAAGCAAGGAATGCTTGTTTGGATATAGAGCCAAGAAGGTCTTCCCGTCAACGCAATTCAGTTCAAACATATCGTGATGATGTTGACATAGACCTTCCGCCTTTGAGGAAGAGGTCGAGGTCGAGTTCATCTTTTTGTGGAAGCTACCTTGCGAGGCCGATGGAAGAAGTCAGACTTTGTTCTTATGAAGAAAGGCAAGCTGCTCTTAAAGCTGCAGAGAAGCTTCTGGAGAATCTACAAACTGATAATCCATCTTTCGTTAAAACAATGGTTCGATCGCATGTTTATAGCTGTTTTTGGTTGGGTCTTCCCACAAGATTTTGTGATGAACATCTCTCAAAAACGGGCTCAGATATGGTGCTGGAGGATGAAAATGGCAACGAGTACGATGCTGTCTACATTGGCTCAAGAACTGGGCTTAGTGGGGGCTGGAGAGCGTTTGCACTGGAACACAAGTTGGATGATGGTGATGCGCTTGTGTTTGAACTGACTGAACCCACAAGATTTAAGATCTACATTGTCAAAGTATCATCAATGTCTAGTATAAAAAGCATTGTGGACAAAGAAGATATTCCCAGTGCTGAAAATACATCGAAGACAGCCGTGAAGCGTGACTCAGAATCAACTCAAAAACGAAGAACTAAGAGAGGTTCTGTGTCTGAGATTGATGACTCACAACCTTCCCCGGGCTCAGAATCAGATCAGAGGCGAAGGTCAAAGAGAGGTATTGTACCTCCAACGGATGACGCAAAAGCTGCCACAGACTTGGAATCAAATCAGATACGAAGGTCAAAGAGAGGGGCTGCGCCTGTAGCAGATGACTCAAAAACCAACTTCAACTCTGAATCAAATCAGCTACGAAGGTCAAAGAGAGGGGCTGCGCCTGTAGCAGATGACTCAAAAATGAACTTCAACTCTGAATCAAATCAGCTACGAAGGTCAAGGAGAGGTACGGTGCCTGTAGCAGATGACTCAAAAACCAACTTCAACTCTGAATCAAATCAGCTACGAAGGTCAAGGAGAGGTACTGTGCCTGCAATGGATGACACAAAAGCTTCGCCAGACTTGGGATCAGATCAGAAACGAAGGTCCAAGAGAGGTGTCGTGCCTGAAATGGATGACCTAGAAGCCTCTCCCAAGCTTCCCACAGAGAGCCCCAAAGGTTGTGAAACCGAGCAGGAAGCCTGTGCAATGAAGGTGGATGAAGTTGCTCCTAAACAAGTGAGAAAGAAAGCTAAAGCAAACAGAGTATGTTTCACAGAGAGTCCAGAAGGCTGTGAAGTGAAACCCGAAGAAACAGAGGAGAAACCCGAAGTACCAGAGAAGAAACCTAAAGTGGAGGAGAAGAATGCGAGAGCCAAAGTTGAGAACGAATGTGTAGCCAAGAAACCTAGAAAGAAAGTTGTGAAGCAAAGGCTTTTCCGAAAGAGAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

532

Amino Acids

59.72

Weight (kDa)

9.4

Isoelectric Point (pI)

72.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 139 - 226 1.4e-18 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1114
AccI GTMKAC 1 cut(s) 555
AciI CCGC 2 cut(s) 15, 257
AclWI GGATC 1 cut(s) 1237
AcsI RAATTY 1 cut(s) 68
AfaI GTAC 5 cut(s) 545, 891, 1111, 1186, 1476
AfiI CCNNNNNNNGG 2 cut(s) 845, 1226
AgsI TTSAA 6 cut(s) 99, 224, 644, 997, 1072, 1147
AjuI GAANNNNNNNTTGG 4 cut(s) 161, 184, 193, 216
Alw26I GTCTC 2 cut(s) 53, 154
AlwI GGATC 1 cut(s) 1237
Ama87I CYCGRG 1 cut(s) 844
AoxI GGCC 1 cut(s) 314
ApeKI GCWGC 5 cut(s) 359, 371, 914, 962, 1037
ApoI RAATTY 1 cut(s) 68
Asp700I GAANNNNTTC 2 cut(s) 199, 729
AspLEI GCGC 3 cut(s) 634, 967, 1042
AspS9I GGNCC 1 cut(s) 1248
AsuC2I CCSGG 2 cut(s) 845, 846
AsuHPI GGTGA 1 cut(s) 638
AvaI CYCGRG 1 cut(s) 844
AvaII GGWCC 1 cut(s) 1248
BaeI ACNNNNGTAYC 2 cut(s) 932, 965
BanI GGYRCC 1 cut(s) 1114
BanII GRGCYC 3 cut(s) 509, 851, 1316
BbsI GAAGAC 3 cut(s) 192, 458, 761
BbvI GCAGC 5 cut(s) 346, 358, 901, 949, 1024
BccI CCATC 4 cut(s) 17, 313, 418, 617
BceAI ACGGC 1 cut(s) 746
BcgI CGANNNNNNTGC 2 cut(s) 530, 564
BcnI CCSGG 2 cut(s) 845, 846
BcoDI GTCTC 2 cut(s) 53, 154
BfaI CTAG 3 cut(s) 699, 1283, 1551
BfmI CTRYAG 4 cut(s) 372, 969, 1044, 1119
BglII AGATCT 1 cut(s) 669
BisI GCNGC 6 cut(s) 15, 360, 372, 915, 963, 1038
BlsI GCNGC 6 cut(s) 16, 361, 373, 916, 964, 1039
Bme1390I CCNGG 2 cut(s) 845, 846
Bme18I GGWCC 1 cut(s) 1248
BmeT110I CYCGRG 1 cut(s) 844
BmgT120I GGNCC 1 cut(s) 1248
BmiI GGNNCC 1 cut(s) 1116
BmrFI CCNGG 2 cut(s) 845, 846
BmrI ACTGGG 2 cut(s) 582, 729
BmsI GCATC 3 cut(s) 151, 538, 619
BmuI ACTGGG 2 cut(s) 582, 729
BpiI GAAGAC 3 cut(s) 192, 458, 761
BpmI CTGGAG 3 cut(s) 407, 542, 610
BpuEI CTTGAG 2 cut(s) 73, 550
BpuMI CCSGG 2 cut(s) 845, 846
BsaBI GATNNNNATC 1 cut(s) 489
BsaJI CCNNGG 2 cut(s) 843, 844
BsaXI ACNNNNNCTCC 2 cut(s) 109, 139
Bsc4I CCNNNNNNNGG 2 cut(s) 845, 1226
Bse1I ACTGG 3 cut(s) 577, 609, 735
Bse3DI GCAATG 2 cut(s) 1203, 1356
Bse8I GATNNNNATC 1 cut(s) 489
BseDI CCNNGG 2 cut(s) 843, 844
BseGI GGATG 6 cut(s) 532, 625, 907, 1207, 1282, 1366
BseJI GATNNNNATC 1 cut(s) 489
BseLI CCNNNNNNNGG 2 cut(s) 845, 1226
BseMI GCAATG 2 cut(s) 1203, 1356
BseMII CTCAG 4 cut(s) 522, 789, 810, 864
BseNI ACTGG 3 cut(s) 577, 609, 735
BseRI GAGGAG 2 cut(s) 1475, 1514
BseXI GCAGC 5 cut(s) 346, 358, 901, 949, 1024
BseYI CCCAGC 1 cut(s) 138
Bsh1285I CGRYCG 1 cut(s) 437
BshFI GGCC 1 cut(s) 316
BshNI GGYRCC 1 cut(s) 1114
BsiEI CGRYCG 1 cut(s) 437
BsiHKCI CYCGRG 1 cut(s) 844
BsiSI CCGG 1 cut(s) 845
BslI CCNNNNNNNGG 2 cut(s) 845, 1226
BsmAI GTCTC 2 cut(s) 53, 154
BsmBI CGTCTC 1 cut(s) 53
BsmI GAATGC 2 cut(s) 175, 1513
BsnI GGCC 1 cut(s) 316
BsoBI CYCGRG 1 cut(s) 844
Bsp1286I GDGCHC 3 cut(s) 509, 851, 1316
Bsp143I GATC 5 cut(s) 434, 669, 859, 1229, 1234
BspACI CCGC 2 cut(s) 15, 257
BspANI GGCC 1 cut(s) 316
BspCNI CTCAG 4 cut(s) 521, 788, 811, 863
BspLI GGNNCC 1 cut(s) 1116
BspMAI CTGCAG 1 cut(s) 376
BspPI GGATC 1 cut(s) 1237
BspT107I GGYRCC 1 cut(s) 1114
BsrDI GCAATG 2 cut(s) 1203, 1356
BsrI ACTGG 3 cut(s) 577, 609, 735
BssECI CCNNGG 2 cut(s) 843, 844
BssMI GATC 5 cut(s) 434, 669, 859, 1229, 1234
Bst4CI ACNGT 2 cut(s) 1114, 1189
Bst6I CTCTTC 1 cut(s) 263
BstAPI GCANNNNNTGC 2 cut(s) 170, 914
BstC8I GCNNGC 3 cut(s) 37, 357, 1194
BstDEI CTNAG 6 cut(s) 508, 578, 775, 801, 819, 850
BstF5I GGATG 6 cut(s) 532, 625, 907, 1207, 1282, 1366
BstHHI GCGC 3 cut(s) 634, 967, 1042
BstKTI GATC 5 cut(s) 437, 672, 862, 1232, 1237
BstMAI GTCTC 2 cut(s) 53, 154
BstMBI GATC 5 cut(s) 434, 669, 859, 1229, 1234
BstMCI CGRYCG 1 cut(s) 437
BstMWI GCNNNNNNNGC 6 cut(s) 14, 170, 368, 914, 971, 1046
BstNSI RCATGY 1 cut(s) 443
BstSCI CCNGG 2 cut(s) 843, 844
BstSFI CTRYAG 4 cut(s) 372, 969, 1044, 1119
BstV1I GCAGC 5 cut(s) 346, 358, 901, 949, 1024
BstV2I GAAGAC 3 cut(s) 192, 458, 761
BstX2I RGATCY 1 cut(s) 669
BstXI CCANNNNNNTGG 1 cut(s) 925
BstYI RGATCY 1 cut(s) 669
BsuRI GGCC 1 cut(s) 316
BtsCI GGATG 6 cut(s) 532, 625, 907, 1207, 1282, 1366
BtsIMutI CAGTG 2 cut(s) 602, 742
Cac8I GCNNGC 3 cut(s) 37, 357, 1194
CfoI GCGC 3 cut(s) 634, 967, 1042
Cfr13I GGNCC 1 cut(s) 1248
Cfr9I CCCGGG 1 cut(s) 844
CseI GACGC 2 cut(s) 35, 914
Csp6I GTAC 5 cut(s) 544, 890, 1110, 1185, 1475
CspCI CAANNNNNGTGG 2 cut(s) 1338, 1373
CviAII CATG 1 cut(s) 440
CviQI GTAC 5 cut(s) 544, 890, 1110, 1185, 1475
DdeI CTNAG 6 cut(s) 508, 578, 775, 801, 819, 850
DpnI GATC 5 cut(s) 436, 671, 861, 1231, 1236
DpnII GATC 5 cut(s) 434, 669, 859, 1229, 1234
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
EciI GGCGGA 1 cut(s) 246
Eco24I GRGCYC 3 cut(s) 509, 851, 1316
Eco47I GGWCC 1 cut(s) 1248
Eco88I CYCGRG 1 cut(s) 844
EcoT38I GRGCYC 3 cut(s) 509, 851, 1316
Esp3I CGTCTC 1 cut(s) 53
FaeI CATG 1 cut(s) 443
FalI AAGNNNNNCTT 8 cut(s) 157, 189, 184, 216, 318, 350, 348, 380
FatI CATG 1 cut(s) 439
FblI GTMKAC 1 cut(s) 555
Fnu4HI GCNGC 6 cut(s) 15, 360, 372, 915, 963, 1038
FokI GGATG 6 cut(s) 539, 632, 914, 1214, 1289, 1373
FriOI GRGCYC 3 cut(s) 509, 851, 1316
Fsp4HI GCNGC 6 cut(s) 15, 360, 372, 915, 963, 1038
FspBI CTAG 3 cut(s) 699, 1283, 1551
GlaI GCGC 3 cut(s) 633, 966, 1041
GluI GCNGC 6 cut(s) 15, 360, 372, 915, 963, 1038
GsaI CCCAGC 1 cut(s) 142
GsuI CTGGAG 3 cut(s) 407, 542, 610
HaeIII GGCC 1 cut(s) 316
HapII CCGG 1 cut(s) 845
HgaI GACGC 2 cut(s) 35, 914
HhaI GCGC 3 cut(s) 634, 967, 1042
Hin1II CATG 1 cut(s) 443
Hin6I GCGC 3 cut(s) 632, 965, 1040
HinP1I GCGC 3 cut(s) 632, 965, 1040
HincII GTYRAC 2 cut(s) 209, 244
HindII GTYRAC 2 cut(s) 209, 244
HindIII AAGCTT 3 cut(s) 380, 1212, 1298
HpaII CCGG 1 cut(s) 845
HphI GGTGA 1 cut(s) 638
Hpy166II GTNNAC 4 cut(s) 209, 244, 556, 718
Hpy188III TCNNGA 7 cut(s) 50, 74, 151, 233, 386, 567, 1427
Hpy8I GTNNAC 4 cut(s) 209, 244, 556, 718
HpyCH4III ACNGT 2 cut(s) 1114, 1189
HpyCH4V TGCA 4 cut(s) 374, 602, 1196, 1349
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 170, 368, 914, 971, 1046
HpyF3I CTNAG 6 cut(s) 508, 578, 775, 801, 819, 850
Hsp92II CATG 1 cut(s) 443
HspAI GCGC 3 cut(s) 632, 965, 1040
Kzo9I GATC 5 cut(s) 434, 669, 859, 1229, 1234
LmnI GCTCC 1 cut(s) 1375
Lsp1109I GCAGC 5 cut(s) 346, 358, 901, 949, 1024
LweI GCATC 3 cut(s) 151, 538, 619
MaeI CTAG 3 cut(s) 699, 1283, 1551
MaeIII GTNAC 1 cut(s) 770
MalI GATC 5 cut(s) 436, 671, 861, 1231, 1236
MboI GATC 5 cut(s) 434, 669, 859, 1229, 1234
MflI RGATCY 1 cut(s) 669
MhlI GDGCHC 3 cut(s) 509, 851, 1316
MluCI AATT 2 cut(s) 68, 214
MlyI GAGTC 6 cut(s) 767, 824, 974, 1049, 1124, 1432
MmeI TCCRAC 2 cut(s) 597, 920
MroXI GAANNNNTTC 2 cut(s) 199, 729
MseI TTAA 3 cut(s) 366, 420, 666
MspI CCGG 1 cut(s) 845
MspR9I CCNGG 2 cut(s) 845, 846
Mva1269I GAATGC 2 cut(s) 175, 1513
MwoI GCNNNNNNNGC 6 cut(s) 14, 170, 368, 914, 971, 1046
NciI CCSGG 2 cut(s) 845, 846
NdeII GATC 5 cut(s) 434, 669, 859, 1229, 1234
NlaIII CATG 1 cut(s) 443
NlaIV GGNNCC 1 cut(s) 1116
NmuCI GTSAC 1 cut(s) 770
NspI RCATGY 1 cut(s) 443
PcsI WCGNNNNNNNCGW 1 cut(s) 43
PctI GAATGC 2 cut(s) 175, 1513
PdmI GAANNNNTTC 2 cut(s) 199, 729
PfeI GAWTC 8 cut(s) 84, 391, 779, 854, 929, 1004, 1079, 1154
PkrI GCNGC 6 cut(s) 16, 361, 373, 916, 964, 1039
Ple19I CGATCG 1 cut(s) 437
PleI GAGTC 6 cut(s) 767, 824, 974, 1049, 1124, 1431
PpsI GAGTC 6 cut(s) 767, 824, 974, 1049, 1124, 1431
PspFI CCCAGC 1 cut(s) 138
PspN4I GGNNCC 1 cut(s) 1116
PspPI GGNCC 1 cut(s) 1248
PstI CTGCAG 1 cut(s) 376
PsuI RGATCY 1 cut(s) 669
PvuI CGATCG 1 cut(s) 437
RsaI GTAC 5 cut(s) 545, 891, 1111, 1186, 1476
RsaNI GTAC 5 cut(s) 544, 890, 1110, 1185, 1475
SaqAI TTAA 3 cut(s) 366, 420, 666
SatI GCNGC 6 cut(s) 15, 360, 372, 915, 963, 1038
Sau3AI GATC 5 cut(s) 434, 669, 859, 1229, 1234
Sau96I GGNCC 1 cut(s) 1248
SchI GAGTC 6 cut(s) 767, 824, 974, 1049, 1124, 1432
ScrFI CCNGG 2 cut(s) 845, 846
SduI GDGCHC 3 cut(s) 509, 851, 1316
SfaNI GCATC 3 cut(s) 151, 538, 619
SfcI CTRYAG 4 cut(s) 372, 969, 1044, 1119
SinI GGWCC 1 cut(s) 1248
SmaI CCCGGG 1 cut(s) 846
SmlI CTYRAG 2 cut(s) 88, 565
SmoI CTYRAG 2 cut(s) 88, 565
Sse9I AATT 2 cut(s) 68, 214
SsiI CCGC 2 cut(s) 15, 257
SspMI CTAG 3 cut(s) 699, 1283, 1551
StyD4I CCNGG 2 cut(s) 843, 844
TaaI ACNGT 2 cut(s) 1114, 1189
TaqI TCGA 6 cut(s) 51, 152, 275, 281, 433, 752
TasI AATT 2 cut(s) 68, 214
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 8 cut(s) 84, 391, 779, 854, 929, 1004, 1079, 1154
Tru1I TTAA 3 cut(s) 366, 420, 666
Tru9I TTAA 3 cut(s) 366, 420, 666
TscAI CASTG 2 cut(s) 609, 742
TseFI GTSAC 1 cut(s) 770
TseI GCWGC 5 cut(s) 359, 371, 914, 962, 1037
Tsp45I GTSAC 1 cut(s) 770
TspDTI ATGAA 7 cut(s) 276, 360, 501, 543, 1079, 1367, 1377
TspGWI ACGGA 1 cut(s) 914
TspMI CCCGGG 1 cut(s) 844
TspRI CASTG 2 cut(s) 609, 742
VpaK11BI GGWCC 1 cut(s) 1248
XapI RAATTY 1 cut(s) 68
XceI RCATGY 1 cut(s) 443
XmaI CCCGGG 1 cut(s) 844
XmiI GTMKAC 1 cut(s) 555
XmnI GAANNNNTTC 2 cut(s) 199, 729
XspI CTAG 3 cut(s) 699, 1283, 1551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.