AT5G58280

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007892
Physical Location & Seq
Forward (+)
23567300 .. 23568859
1560 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G58280.1

Sequence Viewer

Length: 822 bp
ATGGCCATGGCCTATGAGGAAGCTCGCAAGCTCCGTCTTCAAGAGAATCACAAGCGATTTCAATCCAATTTTGTAGCTTTACCACAATTTCAGCTTCTTCAGCAGCGTAATCACAAACCCATCGATAAAGCCCTCTTAGCTACTGCTGAACCAAGACGCTCTTCCCGTGTTCGTACCGTAATTTCTTCCTATCGCGATGATGTCGTCGTAGACACTGGTCGTACTTCGAATCTGAGGAGATCGCGACATAGCTCTACATGGGCGACCTACATTTCTCGACCTCTCCATGAGTGCAAGTTTGCATCTTATGAAGAAAAGGTTGGAGCATTCAAGGCTGCAGAGAAGTTCCAGAGGAGCCTTAAATCTCCACATCCTTACTTTGTGAAATCTATGGTTCGCTCCCATGTTTACAGTTGCTTTTGGCTGGGGCTTCCTTCAAGATTCTGTGCAGACAATTTTCCTGAGGAAACTATGGAAATAGAACTAGAGGATGAAGAAGGCGAAGTGTATGAAGCAGTGTACATTGGTAGAAGAGCTGGACTCAGTGGTGGTTGGAAAAGGTTTGCTCTTGATCATAAATTGGATGATGGTGATGCTCTACTCTTTGAATTGGTTGAACCAAAGAAGTTCAAGATCTATGTGTTTAAAGGAAATGAGAACGCTAATCTTACAAGCGCCAGAAAGAGAGGCAGAGCAACGACGCCAAGTGAAGAAGAAGAAGAAGAAGAAGATAAGGATGTTGAAGAATCAGGTGATGAGGAACACAGCTCAAGAGCCACTAAGAGATCATCCGTAAGGTTACTAAGGAAGAGGAAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

273

Amino Acids

31.67

Weight (kDa)

8.97

Isoelectric Point (pI)

68.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 127 - 216 2.5e-19 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 210
AccII CGCG 2 cut(s) 195, 244
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 83
AcyI GRCGYC 1 cut(s) 701
AfaI GTAC 3 cut(s) 175, 223, 521
AgsI TTSAA 8 cut(s) 41, 62, 331, 438, 608, 617, 631, 743
AjuI GAANNNNNNNTTGG 4 cut(s) 145, 177, 303, 335
AluBI AGCT 8 cut(s) 23, 31, 77, 94, 140, 252, 536, 768
AluI AGCT 8 cut(s) 23, 31, 77, 94, 140, 252, 536, 768
AoxI GGCC 2 cut(s) 3, 9
ApeKI GCWGC 2 cut(s) 103, 335
AspLEI GCGC 1 cut(s) 677
AsuHPI GGTGA 2 cut(s) 602, 764
AsuII TTCGAA 1 cut(s) 227
AxyI CCTNAGG 1 cut(s) 462
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 29
BbvI GCAGC 2 cut(s) 115, 322
BccI CCATC 2 cut(s) 128, 581
BclI TGATCA 1 cut(s) 571
BfaI CTAG 1 cut(s) 485
BfmI CTRYAG 1 cut(s) 336
BfoI RGCGCY 1 cut(s) 678
BglII AGATCT 1 cut(s) 633
BisI GCNGC 2 cut(s) 104, 336
BlsI GCNGC 2 cut(s) 105, 337
BmiI GGNNCC 1 cut(s) 356
BmsI GCATC 2 cut(s) 311, 583
BoxI GACNNNNGTC 1 cut(s) 216
BpiI GAAGAC 1 cut(s) 29
BplI GAGNNNNNCTC 2 cut(s) 525, 557
Bpu14I TTCGAA 1 cut(s) 227
BpuEI CTTGAG 1 cut(s) 754
Bsa29I ATCGAT 1 cut(s) 123
BsaBI GATNNNNATC 1 cut(s) 61
BsaHI GRCGYC 1 cut(s) 701
BsaJI CCNNGG 1 cut(s) 6
Bse1I ACTGG 1 cut(s) 220
Bse21I CCTNAGG 1 cut(s) 462
Bse8I GATNNNNATC 1 cut(s) 61
BseCI ATCGAT 1 cut(s) 123
BseDI CCNNGG 1 cut(s) 6
BseGI GGATG 5 cut(s) 370, 496, 589, 742, 788
BseJI GATNNNNATC 1 cut(s) 61
BseMII CTCAG 3 cut(s) 224, 453, 556
BseNI ACTGG 1 cut(s) 220
BseRI GAGGAG 2 cut(s) 250, 367
BseXI GCAGC 2 cut(s) 115, 322
BseYI CCCAGC 1 cut(s) 424
BsgI GTGCAG 1 cut(s) 468
Bsh1236I CGCG 2 cut(s) 195, 244
BshFI GGCC 2 cut(s) 5, 11
BshVI ATCGAT 1 cut(s) 123
BsmI GAATGC 1 cut(s) 326
BsnI GGCC 2 cut(s) 5, 11
Bsp119I TTCGAA 1 cut(s) 227
Bsp1407I TGTACA 1 cut(s) 519
Bsp143I GATC 4 cut(s) 239, 571, 633, 785
Bsp19I CCATGG 1 cut(s) 6
Bsp68I TCGCGA 2 cut(s) 195, 244
BspANI GGCC 2 cut(s) 5, 11
BspCNI CTCAG 3 cut(s) 225, 454, 555
BspDI ATCGAT 1 cut(s) 123
BspFNI CGCG 2 cut(s) 195, 244
BspLI GGNNCC 1 cut(s) 356
BspMAI CTGCAG 1 cut(s) 340
BspQI GCTCTTC 2 cut(s) 166, 526
BspT104I TTCGAA 1 cut(s) 227
BsrGI TGTACA 1 cut(s) 519
BsrI ACTGG 1 cut(s) 220
BssECI CCNNGG 1 cut(s) 6
BssMI GATC 4 cut(s) 239, 571, 633, 785
BssNI GRCGYC 1 cut(s) 701
BssT1I CCWWGG 1 cut(s) 6
Bst4CI ACNGT 2 cut(s) 178, 413
Bst6I CTCTTC 3 cut(s) 166, 526, 803
BstACI GRCGYC 1 cut(s) 701
BstAUI TGTACA 1 cut(s) 519
BstBI TTCGAA 1 cut(s) 227
BstC8I GCNNGC 2 cut(s) 25, 29
BstDEI CTNAG 6 cut(s) 136, 233, 462, 542, 780, 803
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 5 cut(s) 370, 496, 589, 742, 788
BstFNI CGCG 2 cut(s) 195, 244
BstH2I RGCGCY 1 cut(s) 678
BstHHI GCGC 1 cut(s) 677
BstKTI GATC 4 cut(s) 242, 574, 636, 788
BstMBI GATC 4 cut(s) 239, 571, 633, 785
BstMWI GCNNNNNNNGC 3 cut(s) 100, 137, 332
BstPAI GACNNNNGTC 1 cut(s) 216
BstSFI CTRYAG 1 cut(s) 336
BstUI CGCG 2 cut(s) 195, 244
BstV1I GCAGC 2 cut(s) 115, 322
BstV2I GAAGAC 1 cut(s) 29
BstX2I RGATCY 1 cut(s) 633
BstYI RGATCY 1 cut(s) 633
Bsu15I ATCGAT 1 cut(s) 123
Bsu36I CCTNAGG 1 cut(s) 462
BsuRI GGCC 2 cut(s) 5, 11
BsuTUI ATCGAT 1 cut(s) 123
BtgI CCRYGG 1 cut(s) 6
BtgZI GCGATG 1 cut(s) 210
BtsCI GGATG 5 cut(s) 370, 496, 589, 742, 788
BtsI GCAGTG 1 cut(s) 522
BtsIMutI CAGTG 3 cut(s) 213, 522, 550
BtuMI TCGCGA 2 cut(s) 195, 244
Cac8I GCNNGC 2 cut(s) 25, 29
CfoI GCGC 1 cut(s) 677
ClaI ATCGAT 1 cut(s) 123
CseI GACGC 2 cut(s) 165, 709
Csp6I GTAC 3 cut(s) 174, 222, 520
CviAII CATG 4 cut(s) 7, 258, 287, 404
CviQI GTAC 3 cut(s) 174, 222, 520
DdeI CTNAG 6 cut(s) 136, 233, 462, 542, 780, 803
DpnI GATC 4 cut(s) 241, 573, 635, 787
DpnII GATC 4 cut(s) 239, 571, 633, 785
DraI TTTAAA 1 cut(s) 646
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 166, 526, 803
EarI CTCTTC 3 cut(s) 166, 526, 803
Eco130I CCWWGG 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 83
Eco81I CCTNAGG 1 cut(s) 462
EcoT14I CCWWGG 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 6
FaeI CATG 4 cut(s) 10, 261, 290, 407
FalI AAGNNNNNCTT 2 cut(s) 145, 177
FatI CATG 4 cut(s) 6, 257, 286, 403
FbaI TGATCA 1 cut(s) 571
FblI GTMKAC 1 cut(s) 210
Fnu4HI GCNGC 2 cut(s) 104, 336
FokI GGATG 5 cut(s) 357, 503, 596, 749, 775
Fsp4HI GCNGC 2 cut(s) 104, 336
FspBI CTAG 1 cut(s) 485
GlaI GCGC 1 cut(s) 676
GluI GCNGC 2 cut(s) 104, 336
GsaI CCCAGC 1 cut(s) 428
HaeII RGCGCY 1 cut(s) 678
HaeIII GGCC 2 cut(s) 5, 11
HgaI GACGC 2 cut(s) 165, 709
HhaI GCGC 1 cut(s) 677
Hin1I GRCGYC 1 cut(s) 701
Hin1II CATG 4 cut(s) 10, 261, 290, 407
Hin6I GCGC 1 cut(s) 675
HinP1I GCGC 1 cut(s) 675
HinfI GANTC 5 cut(s) 46, 229, 441, 540, 746
HphI GGTGA 2 cut(s) 602, 764
Hpy166II GTNNAC 3 cut(s) 211, 409, 520
Hpy188I TCNGA 1 cut(s) 234
Hpy8I GTNNAC 3 cut(s) 211, 409, 520
Hpy99I CGWCG 2 cut(s) 209, 703
HpyAV CCTTC 2 cut(s) 444, 491
HpyCH4III ACNGT 2 cut(s) 178, 413
HpyCH4V TGCA 4 cut(s) 294, 302, 338, 449
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 137, 332
HpyF3I CTNAG 6 cut(s) 136, 233, 462, 542, 780, 803
Hsp92I GRCGYC 1 cut(s) 701
Hsp92II CATG 4 cut(s) 10, 261, 290, 407
HspAI GCGC 1 cut(s) 675
Ksp22I TGATCA 1 cut(s) 571
Kzo9I GATC 4 cut(s) 239, 571, 633, 785
LguI GCTCTTC 2 cut(s) 166, 526
LmnI GCTCC 4 cut(s) 36, 323, 354, 404
LpnPI CCDG 7 cut(s) 201, 362, 410, 474, 522, 691, 735
Lsp1109I GCAGC 2 cut(s) 115, 322
LweI GCATC 2 cut(s) 311, 583
MaeI CTAG 1 cut(s) 485
MaeIII GTNAC 1 cut(s) 798
MalI GATC 4 cut(s) 241, 573, 635, 787
MboI GATC 4 cut(s) 239, 571, 633, 785
MflI RGATCY 1 cut(s) 633
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 67, 86, 180, 454, 578, 608
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 534
MmeI TCCRAC 2 cut(s) 301, 533
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 360, 645
Msp20I TGGCCA 1 cut(s) 5
Mva1269I GAATGC 1 cut(s) 326
MvnI CGCG 2 cut(s) 195, 244
MwoI GCNNNNNNNGC 3 cut(s) 100, 137, 332
NcoI CCATGG 1 cut(s) 6
NdeII GATC 4 cut(s) 239, 571, 633, 785
NlaIII CATG 4 cut(s) 10, 261, 290, 407
NlaIV GGNNCC 1 cut(s) 356
NruI TCGCGA 2 cut(s) 195, 244
NspV TTCGAA 1 cut(s) 227
PciSI GCTCTTC 2 cut(s) 166, 526
PcsI WCGNNNNNNNCGW 2 cut(s) 31, 163
PctI GAATGC 1 cut(s) 326
PfeI GAWTC 4 cut(s) 46, 229, 441, 746
PkrI GCNGC 2 cut(s) 105, 337
PleI GAGTC 1 cut(s) 534
PpsI GAGTC 1 cut(s) 534
PshAI GACNNNNGTC 1 cut(s) 216
PspFI CCCAGC 1 cut(s) 424
PspN4I GGNNCC 1 cut(s) 356
PstI CTGCAG 1 cut(s) 340
PsuI RGATCY 1 cut(s) 633
RruI TCGCGA 2 cut(s) 195, 244
RsaI GTAC 3 cut(s) 175, 223, 521
RsaNI GTAC 3 cut(s) 174, 222, 520
SapI GCTCTTC 2 cut(s) 166, 526
SaqAI TTAA 2 cut(s) 360, 645
SatI GCNGC 2 cut(s) 104, 336
Sau3AI GATC 4 cut(s) 239, 571, 633, 785
SchI GAGTC 1 cut(s) 534
SfaNI GCATC 2 cut(s) 311, 583
SfcI CTRYAG 1 cut(s) 336
SfuI TTCGAA 1 cut(s) 227
SmlI CTYRAG 1 cut(s) 769
SmoI CTYRAG 1 cut(s) 769
Sse9I AATT 6 cut(s) 67, 86, 180, 454, 578, 608
SspMI CTAG 1 cut(s) 485
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 2 cut(s) 178, 413
TaqI TCGA 3 cut(s) 123, 227, 277
TasI AATT 6 cut(s) 67, 86, 180, 454, 578, 608
TatI WGTACW 1 cut(s) 519
TfiI GAWTC 4 cut(s) 46, 229, 441, 746
Tru1I TTAA 2 cut(s) 360, 645
Tru9I TTAA 2 cut(s) 360, 645
TscAI CASTG 3 cut(s) 220, 522, 550
TseI GCWGC 2 cut(s) 103, 335
TspDTI ATGAA 3 cut(s) 324, 507, 525
TspGWI ACGGA 2 cut(s) 23, 781
TspRI CASTG 3 cut(s) 220, 522, 550
XmiI GTMKAC 1 cut(s) 210
XspI CTAG 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.