RLG00000036404

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0007892
Physical Location & Seq
Reverse (-)
80073272 .. 80075793
2522 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036404

Sequence Viewer

Length: 885 bp
ATGGTAGCCGCTTACGAAGAAGCTCGTAACAAACGCCTTGAAGAGAACAAGAAGAAGTTTCAGGATTTGGGAATCACCCAGGTTTCAAAAACTCTGTCTGACCTCACAGCTTCTGAGAAGAAGCCCCAGCAAAGGGCTTGGAAGCCGAAAGCAAAGAACACTGCTTTCGAAGTGGAGCCAAGACGATCCACCCGTCAGCGAAACCCAATTCAAACATATGTTGATGATGTTGACATAGGCCTTCCAACTAGGAAGAGGTTGAGGTCAAGTTCATCTTCATTTGGAAGCTACCTTGCAAGGCCTTTGGAGGAATGCCGAATGGCTTCTTATGAAGAAAGGAGTGCTGCTTTCAAAGCTGCAGAGCGGCTTCAAGAGAGTCTGCACTCTGAAAATCCATCTTTTGTCAAATCGATGGTTCGATCTCATGTCTATAGCTGTTTTTGGTTGGGTCTTCCTTCTAATTTCTGTGAGACTCATCTCCCAAAATCATCGGACTCTGAACTGATACTAGAGGATGAAGATGGAAATGAATATGATGCTAAATACATTGCCAAGAGGACTGGTCTTAGTGGTGGTTGGAGAGGTTTTGCATTGGAACACAAGTTGGACGATGGTGATGCACTTGTGTTTGAATTGGTTGAACCCACAAGGTTTAAGATCTACATAGTTAAGGTGACAACTCAAGAACATTGCAAGGACAAAGTGAAGGGTGCTGGAAAGCCATCAAAGGCAGCCAAGAAGCCTGACCAAGACTCTAACAAAAAACAAAGGTCAAAGAAAGCTACCACTGCAGAAATAATCGACATGAAAACACCCCCAGAGCTTCTACCAGAGAGCCCAAAAAGTGAAGCTGTACTACCATATAGACTCAGGAAGAGAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

295

Amino Acids

33.45

Weight (kDa)

9.38

Isoelectric Point (pI)

55.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 134 - 222 3.2e-19 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 364
AciI CCGC 2 cut(s) 9, 364
AclWI GGATC 1 cut(s) 180
AfaI GTAC 1 cut(s) 855
AfiI CCNNNNNNNGG 2 cut(s) 132, 133
AgsI TTSAA 7 cut(s) 41, 87, 212, 352, 371, 632, 641
AjnI CCWGG 1 cut(s) 78
AluBI AGCT 8 cut(s) 23, 110, 288, 356, 435, 782, 823, 851
AluI AGCT 8 cut(s) 23, 110, 288, 356, 435, 782, 823, 851
Alw26I GTCTC 1 cut(s) 464
AlwI GGATC 1 cut(s) 180
AlwNI CAGNNNCTG 1 cut(s) 113
AoxI GGCC 2 cut(s) 238, 299
ApeKI GCWGC 3 cut(s) 344, 356, 731
ArsI GACNNNNNNTTYG 2 cut(s) 80, 112
Asp700I GAANNNNTTC 1 cut(s) 322
AsuHPI GGTGA 3 cut(s) 67, 626, 685
AsuII TTCGAA 1 cut(s) 168
BanII GRGCYC 1 cut(s) 839
BarI GAAGNNNNNNTAC 2 cut(s) 840, 872
BbsI GAAGAC 1 cut(s) 443
BbvI GCAGC 3 cut(s) 331, 343, 743
BccI CCATC 5 cut(s) 403, 406, 515, 605, 730
BcgI CGANNNNNNTGC 2 cut(s) 599, 633
BciT130I CCWGG 1 cut(s) 80
BcoDI GTCTC 1 cut(s) 464
BfaI CTAG 2 cut(s) 249, 509
BfmI CTRYAG 3 cut(s) 357, 430, 789
BglII AGATCT 1 cut(s) 657
BisI GCNGC 5 cut(s) 9, 345, 357, 365, 732
BlsI GCNGC 5 cut(s) 10, 346, 358, 366, 733
Bme1390I CCNGG 1 cut(s) 80
BmiI GGNNCC 1 cut(s) 177
BmrFI CCNGG 1 cut(s) 80
BmsI GCATC 2 cut(s) 526, 607
BpiI GAAGAC 1 cut(s) 443
Bpu14I TTCGAA 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 666
Bsa29I ATCGAT 1 cut(s) 410
BsaJI CCNNGG 1 cut(s) 78
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 133
Bse1I ACTGG 1 cut(s) 565
Bse3DI GCAATG 2 cut(s) 546, 688
BseBI CCWGG 1 cut(s) 80
BseCI ATCGAT 1 cut(s) 410
BseDI CCNNGG 1 cut(s) 78
BseGI GGATG 1 cut(s) 520
BseLI CCNNNNNNNGG 2 cut(s) 132, 133
BseMI GCAATG 2 cut(s) 546, 688
BseMII CTCAG 2 cut(s) 105, 883
BseNI ACTGG 1 cut(s) 565
BseXI GCAGC 3 cut(s) 331, 343, 743
BseYI CCCAGC 1 cut(s) 126
BsgI GTGCAG 1 cut(s) 365
BshFI GGCC 2 cut(s) 240, 301
BshVI ATCGAT 1 cut(s) 410
BslI CCNNNNNNNGG 2 cut(s) 132, 133
BsmAI GTCTC 1 cut(s) 464
BsmI GAATGC 1 cut(s) 317
BsnI GGCC 2 cut(s) 240, 301
Bsp119I TTCGAA 1 cut(s) 168
Bsp1286I GDGCHC 1 cut(s) 839
Bsp143I GATC 3 cut(s) 185, 419, 657
BspACI CCGC 2 cut(s) 9, 364
BspANI GGCC 2 cut(s) 240, 301
BspCNI CTCAG 2 cut(s) 106, 882
BspDI ATCGAT 1 cut(s) 410
BspLI GGNNCC 1 cut(s) 177
BspMAI CTGCAG 2 cut(s) 361, 793
BspPI GGATC 1 cut(s) 180
BspT104I TTCGAA 1 cut(s) 168
BsrBI CCGCTC 1 cut(s) 364
BsrDI GCAATG 2 cut(s) 546, 688
BsrI ACTGG 1 cut(s) 565
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 3 cut(s) 185, 419, 657
Bst2UI CCWGG 1 cut(s) 80
Bst6I CTCTTC 3 cut(s) 36, 248, 869
BstBI TTCGAA 1 cut(s) 168
BstDEI CTNAG 3 cut(s) 114, 566, 869
BstF5I GGATG 1 cut(s) 520
BstKTI GATC 3 cut(s) 188, 422, 660
BstMAI GTCTC 1 cut(s) 464
BstMBI GATC 3 cut(s) 185, 419, 657
BstMWI GCNNNNNNNGC 2 cut(s) 353, 788
BstNI CCWGG 1 cut(s) 80
BstSCI CCNGG 1 cut(s) 78
BstSFI CTRYAG 3 cut(s) 357, 430, 789
BstV1I GCAGC 3 cut(s) 331, 343, 743
BstV2I GAAGAC 1 cut(s) 443
BstX2I RGATCY 1 cut(s) 657
BstYI RGATCY 1 cut(s) 657
Bsu15I ATCGAT 1 cut(s) 410
BsuRI GGCC 2 cut(s) 240, 301
BsuTUI ATCGAT 1 cut(s) 410
BtsCI GGATG 1 cut(s) 520
BtsI GCAGTG 2 cut(s) 159, 786
BtsIMutI CAGTG 2 cut(s) 159, 786
CaiI CAGNNNCTG 1 cut(s) 113
ClaI ATCGAT 1 cut(s) 410
Csp6I GTAC 1 cut(s) 854
CviAII CATG 2 cut(s) 425, 805
CviQI GTAC 1 cut(s) 854
DdeI CTNAG 3 cut(s) 114, 566, 869
DpnI GATC 3 cut(s) 187, 421, 659
DpnII GATC 3 cut(s) 185, 419, 657
Eam1104I CTCTTC 3 cut(s) 36, 248, 869
EarI CTCTTC 3 cut(s) 36, 248, 869
Eco147I AGGCCT 2 cut(s) 240, 301
Eco24I GRGCYC 1 cut(s) 839
EcoRII CCWGG 1 cut(s) 78
EcoT38I GRGCYC 1 cut(s) 839
FaeI CATG 2 cut(s) 428, 808
FalI AAGNNNNNCTT 2 cut(s) 259, 291
FatI CATG 2 cut(s) 424, 804
FauNDI CATATG 1 cut(s) 217
Fnu4HI GCNGC 5 cut(s) 9, 345, 357, 365, 732
FokI GGATG 1 cut(s) 527
FriOI GRGCYC 1 cut(s) 839
Fsp4HI GCNGC 5 cut(s) 9, 345, 357, 365, 732
FspBI CTAG 2 cut(s) 249, 509
GluI GCNGC 5 cut(s) 9, 345, 357, 365, 732
GsaI CCCAGC 1 cut(s) 130
HaeIII GGCC 2 cut(s) 240, 301
Hin1II CATG 2 cut(s) 428, 808
HincII GTYRAC 1 cut(s) 232
HindII GTYRAC 1 cut(s) 232
HinfI GANTC 6 cut(s) 72, 376, 472, 494, 752, 867
HphI GGTGA 3 cut(s) 67, 626, 685
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 5 cut(s) 100, 115, 388, 493, 499
Hpy188III TCNNGA 4 cut(s) 62, 371, 683, 871
Hpy8I GTNNAC 1 cut(s) 232
HpyAV CCTTC 3 cut(s) 251, 465, 700
HpyCH4V TGCA 7 cut(s) 296, 359, 382, 590, 620, 693, 791
HpyF10VI GCNNNNNNNGC 2 cut(s) 353, 788
HpyF3I CTNAG 3 cut(s) 114, 566, 869
Hsp92II CATG 2 cut(s) 428, 808
Kzo9I GATC 3 cut(s) 185, 419, 657
LmnI GCTCC 1 cut(s) 175
Lsp1109I GCAGC 3 cut(s) 331, 343, 743
LweI GCATC 2 cut(s) 526, 607
MaeI CTAG 2 cut(s) 249, 509
MaeIII GTNAC 2 cut(s) 26, 673
MalI GATC 3 cut(s) 187, 421, 659
MbiI CCGCTC 1 cut(s) 364
MboI GATC 3 cut(s) 185, 419, 657
MboII GAAGA 9 cut(s) 29, 53, 64, 130, 265, 267, 344, 443, 530
MflI RGATCY 1 cut(s) 657
MhlI GDGCHC 1 cut(s) 839
MluCI AATT 3 cut(s) 207, 460, 632
MlyI GAGTC 5 cut(s) 385, 466, 488, 746, 861
MmeI TCCRAC 3 cut(s) 269, 557, 585
MnlI CCTC 7 cut(s) 113, 249, 255, 301, 505, 549, 575
MroXI GAANNNNTTC 1 cut(s) 322
MseI TTAA 2 cut(s) 654, 669
MspR9I CCNGG 1 cut(s) 80
Mva1269I GAATGC 1 cut(s) 317
MvaI CCWGG 1 cut(s) 80
MwoI GCNNNNNNNGC 2 cut(s) 353, 788
NdeI CATATG 1 cut(s) 217
NdeII GATC 3 cut(s) 185, 419, 657
NlaIII CATG 2 cut(s) 428, 808
NlaIV GGNNCC 1 cut(s) 177
NmuCI GTSAC 1 cut(s) 673
NspV TTCGAA 1 cut(s) 168
PceI AGGCCT 2 cut(s) 240, 301
PcsI WCGNNNNNNNCGW 1 cut(s) 190
PctI GAATGC 1 cut(s) 317
PdmI GAANNNNTTC 1 cut(s) 322
PfeI GAWTC 1 cut(s) 72
PkrI GCNGC 5 cut(s) 10, 346, 358, 366, 733
PleI GAGTC 5 cut(s) 384, 466, 488, 746, 861
PpsI GAGTC 5 cut(s) 384, 466, 488, 746, 861
Psp6I CCWGG 1 cut(s) 78
PspFI CCCAGC 1 cut(s) 126
PspGI CCWGG 1 cut(s) 78
PspN4I GGNNCC 1 cut(s) 177
PstI CTGCAG 2 cut(s) 361, 793
PstNI CAGNNNCTG 1 cut(s) 113
PsuI RGATCY 1 cut(s) 657
RsaI GTAC 1 cut(s) 855
RsaNI GTAC 1 cut(s) 854
SaqAI TTAA 2 cut(s) 654, 669
SatI GCNGC 5 cut(s) 9, 345, 357, 365, 732
Sau3AI GATC 3 cut(s) 185, 419, 657
SchI GAGTC 5 cut(s) 385, 466, 488, 746, 861
ScrFI CCNGG 1 cut(s) 80
SduI GDGCHC 1 cut(s) 839
SfaNI GCATC 2 cut(s) 526, 607
SfcI CTRYAG 3 cut(s) 357, 430, 789
SfuI TTCGAA 1 cut(s) 168
SmlI CTYRAG 1 cut(s) 681
SmoI CTYRAG 1 cut(s) 681
Sse9I AATT 3 cut(s) 207, 460, 632
SseBI AGGCCT 2 cut(s) 240, 301
SsiI CCGC 2 cut(s) 9, 364
SspMI CTAG 2 cut(s) 249, 509
StuI AGGCCT 2 cut(s) 240, 301
StyD4I CCNGG 1 cut(s) 78
TaqI TCGA 4 cut(s) 168, 410, 418, 801
TasI AATT 3 cut(s) 207, 460, 632
TatI WGTACW 1 cut(s) 853
TauI GCSGC 2 cut(s) 11, 367
TfiI GAWTC 1 cut(s) 72
Tru1I TTAA 2 cut(s) 654, 669
Tru9I TTAA 2 cut(s) 654, 669
TscAI CASTG 2 cut(s) 166, 793
TseFI GTSAC 1 cut(s) 673
TseI GCWGC 3 cut(s) 344, 356, 731
Tsp45I GTSAC 1 cut(s) 673
TspDTI ATGAA 6 cut(s) 261, 267, 345, 531, 543, 821
TspRI CASTG 2 cut(s) 166, 793
XmnI GAANNNNTTC 1 cut(s) 322
XspI CTAG 2 cut(s) 249, 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.