RchiOBHm_Chr5g0073681

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0007892
Physical Location & Seq
Reverse (-)
79728414 .. 79731422
3009 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34856

Sequence Viewer

Length: 888 bp
ATGGTAGCCGCTTACGAAGAAGCTCGTAACAAACGCCTTGAAGAAAACAAGAAGAAGTTTCAGGATTTGGGAATCACCCAGGTTTCAAAAACTCTGTCTGACCTCACAGCTTCTGAGAAGAAGCCCCAGCAAAGGGCTTGGAAGCCGAAAGCAAAGAACACTGCTTTCGAAGTGGAGCCAAGACGATCCACCCGTCAGCGAAACCCAATTCAAACATATGTTGATGATGTTGACATAGGCCTTCCAACTAGGAAGAGGTTGAGGTCAAGTTCATCTTCATTTGGAAGCTACCTTGCAAGGCCTTTGGAGGAATGCCGAATGGCTTCTTATGAAGAAAGGAGTGCTGCTTTCAAAGCAGCAGAGCGGCTTCAAGAGAGTCTGCAATCTGAGAATCCATCTTTTGTCAAATCGATGGTTCGGTCTCATGTCTATAGCTGTTTTTGGTTGGGTCTTCCTTCTAATTTCTGTGAGACTCATCTCCCAAAATCATCGGACTCTGAACTGATACTAGAGGATGAAGATGGAAATGTATATGATGCTAAATACATTGCCAAGAGGACTGGTCTTAGTGGTGGTTGGAGAGGTTTTGCATTGGAACACAAGTTGGACGATGGTGATGCACTTGTGTTCGAATTGGTTGAACCCACAAGGTTTAAGATCTACATAGTTAAGGTGACAAATCAAGAACATTGCAAGAAGGACAAAGTGAAGAGTGCTGGAAAGCCATCAAAGGCAGCCAAGAAGCCTGACCAAGACCCTAACAAAAAACAAAGGTCAAAGAAAGCTACCACTGCTGAAATAATCGACATGAAAACACCCCCAGAGCTTCTACCAGAGAGCCCAAAAAGTGAAGCTGTACTACCATATAGACTAAGGAAGAGAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

295

Amino Acids

33.59

Weight (kDa)

9.48

Isoelectric Point (pI)

56.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 134 - 222 4.5e-19 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 364
AciI CCGC 2 cut(s) 9, 364
AclWI GGATC 1 cut(s) 180
AfaI GTAC 1 cut(s) 858
AfiI CCNNNNNNNGG 2 cut(s) 132, 133
AgsI TTSAA 6 cut(s) 41, 87, 212, 352, 371, 641
AjnI CCWGG 1 cut(s) 78
AluBI AGCT 7 cut(s) 23, 110, 288, 435, 785, 826, 854
AluI AGCT 7 cut(s) 23, 110, 288, 435, 785, 826, 854
Alw26I GTCTC 2 cut(s) 426, 464
AlwI GGATC 1 cut(s) 180
AlwNI CAGNNNCTG 1 cut(s) 113
AoxI GGCC 2 cut(s) 238, 299
ApeKI GCWGC 3 cut(s) 344, 356, 734
ArsI GACNNNNNNTTYG 2 cut(s) 80, 112
Asp700I GAANNNNTTC 1 cut(s) 322
AsuHPI GGTGA 3 cut(s) 67, 626, 685
AsuII TTCGAA 2 cut(s) 168, 630
BanII GRGCYC 1 cut(s) 842
BarI GAAGNNNNNNTAC 2 cut(s) 843, 875
BbsI GAAGAC 1 cut(s) 443
BbvI GCAGC 3 cut(s) 331, 368, 746
BccI CCATC 5 cut(s) 403, 406, 515, 605, 733
BcgI CGANNNNNNTGC 2 cut(s) 599, 633
BciT130I CCWGG 1 cut(s) 80
BcoDI GTCTC 2 cut(s) 426, 464
BfaI CTAG 2 cut(s) 249, 509
BfmI CTRYAG 1 cut(s) 430
BglII AGATCT 1 cut(s) 657
BisI GCNGC 5 cut(s) 9, 345, 357, 365, 735
BlsI GCNGC 5 cut(s) 10, 346, 358, 366, 736
Bme1390I CCNGG 1 cut(s) 80
BmiI GGNNCC 1 cut(s) 177
BmrFI CCNGG 1 cut(s) 80
BmsI GCATC 2 cut(s) 526, 607
BpiI GAAGAC 1 cut(s) 443
Bpu14I TTCGAA 2 cut(s) 168, 630
Bsa29I ATCGAT 1 cut(s) 410
BsaI GGTCTC 1 cut(s) 426
BsaJI CCNNGG 1 cut(s) 78
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 133
Bse1I ACTGG 1 cut(s) 565
Bse3DI GCAATG 2 cut(s) 546, 688
BseBI CCWGG 1 cut(s) 80
BseCI ATCGAT 1 cut(s) 410
BseDI CCNNGG 1 cut(s) 78
BseGI GGATG 1 cut(s) 520
BseLI CCNNNNNNNGG 2 cut(s) 132, 133
BseMI GCAATG 2 cut(s) 546, 688
BseMII CTCAG 2 cut(s) 105, 378
BseNI ACTGG 1 cut(s) 565
BseXI GCAGC 3 cut(s) 331, 368, 746
BseYI CCCAGC 1 cut(s) 126
BshFI GGCC 2 cut(s) 240, 301
BshVI ATCGAT 1 cut(s) 410
BslI CCNNNNNNNGG 2 cut(s) 132, 133
BsmAI GTCTC 2 cut(s) 426, 464
BsmI GAATGC 1 cut(s) 317
BsnI GGCC 2 cut(s) 240, 301
Bso31I GGTCTC 1 cut(s) 426
Bsp119I TTCGAA 2 cut(s) 168, 630
Bsp1286I GDGCHC 1 cut(s) 842
Bsp143I GATC 2 cut(s) 185, 657
BspACI CCGC 2 cut(s) 9, 364
BspANI GGCC 2 cut(s) 240, 301
BspCNI CTCAG 2 cut(s) 106, 379
BspDI ATCGAT 1 cut(s) 410
BspLI GGNNCC 1 cut(s) 177
BspPI GGATC 1 cut(s) 180
BspT104I TTCGAA 2 cut(s) 168, 630
BspTNI GGTCTC 1 cut(s) 426
BsrBI CCGCTC 1 cut(s) 364
BsrDI GCAATG 2 cut(s) 546, 688
BsrI ACTGG 1 cut(s) 565
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 2 cut(s) 185, 657
Bst2UI CCWGG 1 cut(s) 80
Bst6I CTCTTC 3 cut(s) 248, 704, 872
BstBI TTCGAA 2 cut(s) 168, 630
BstDEI CTNAG 4 cut(s) 114, 387, 566, 872
BstF5I GGATG 1 cut(s) 520
BstKTI GATC 2 cut(s) 188, 660
BstMAI GTCTC 2 cut(s) 426, 464
BstMBI GATC 2 cut(s) 185, 657
BstMWI GCNNNNNNNGC 2 cut(s) 353, 791
BstNI CCWGG 1 cut(s) 80
BstSCI CCNGG 1 cut(s) 78
BstSFI CTRYAG 1 cut(s) 430
BstV1I GCAGC 3 cut(s) 331, 368, 746
BstV2I GAAGAC 1 cut(s) 443
BstX2I RGATCY 1 cut(s) 657
BstYI RGATCY 1 cut(s) 657
Bsu15I ATCGAT 1 cut(s) 410
BsuRI GGCC 2 cut(s) 240, 301
BsuTUI ATCGAT 1 cut(s) 410
BtsCI GGATG 1 cut(s) 520
BtsI GCAGTG 2 cut(s) 159, 789
BtsIMutI CAGTG 2 cut(s) 159, 789
CaiI CAGNNNCTG 1 cut(s) 113
ClaI ATCGAT 1 cut(s) 410
Csp6I GTAC 1 cut(s) 857
CviAII CATG 2 cut(s) 425, 808
CviQI GTAC 1 cut(s) 857
DdeI CTNAG 4 cut(s) 114, 387, 566, 872
DpnI GATC 2 cut(s) 187, 659
DpnII GATC 2 cut(s) 185, 657
Eam1104I CTCTTC 3 cut(s) 248, 704, 872
EarI CTCTTC 3 cut(s) 248, 704, 872
Eco147I AGGCCT 2 cut(s) 240, 301
Eco24I GRGCYC 1 cut(s) 842
Eco31I GGTCTC 1 cut(s) 426
EcoRII CCWGG 1 cut(s) 78
EcoT38I GRGCYC 1 cut(s) 842
FaeI CATG 2 cut(s) 428, 811
FalI AAGNNNNNCTT 2 cut(s) 259, 291
FatI CATG 2 cut(s) 424, 807
FauNDI CATATG 1 cut(s) 217
Fnu4HI GCNGC 5 cut(s) 9, 345, 357, 365, 735
FokI GGATG 1 cut(s) 527
FriOI GRGCYC 1 cut(s) 842
Fsp4HI GCNGC 5 cut(s) 9, 345, 357, 365, 735
FspBI CTAG 2 cut(s) 249, 509
GluI GCNGC 5 cut(s) 9, 345, 357, 365, 735
GsaI CCCAGC 1 cut(s) 130
HaeIII GGCC 2 cut(s) 240, 301
Hin1II CATG 2 cut(s) 428, 811
HincII GTYRAC 1 cut(s) 232
HindII GTYRAC 1 cut(s) 232
HinfI GANTC 5 cut(s) 72, 376, 391, 472, 494
HphI GGTGA 3 cut(s) 67, 626, 685
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 5 cut(s) 100, 115, 388, 493, 499
Hpy188III TCNNGA 3 cut(s) 62, 371, 683
Hpy8I GTNNAC 1 cut(s) 232
HpyAV CCTTC 3 cut(s) 251, 465, 691
HpyCH4V TGCA 5 cut(s) 296, 382, 590, 620, 693
HpyF10VI GCNNNNNNNGC 2 cut(s) 353, 791
HpyF3I CTNAG 4 cut(s) 114, 387, 566, 872
Hsp92II CATG 2 cut(s) 428, 811
Kzo9I GATC 2 cut(s) 185, 657
LmnI GCTCC 1 cut(s) 175
LpnPI CCDG 9 cut(s) 47, 65, 92, 140, 546, 702, 759, 834, 846
Lsp1109I GCAGC 3 cut(s) 331, 368, 746
LweI GCATC 2 cut(s) 526, 607
MaeI CTAG 2 cut(s) 249, 509
MaeIII GTNAC 2 cut(s) 26, 673
MalI GATC 2 cut(s) 187, 659
MbiI CCGCTC 1 cut(s) 364
MboI GATC 2 cut(s) 185, 657
MflI RGATCY 1 cut(s) 657
MhlI GDGCHC 1 cut(s) 842
MluCI AATT 3 cut(s) 207, 460, 632
MlyI GAGTC 3 cut(s) 385, 466, 488
MmeI TCCRAC 3 cut(s) 269, 557, 585
MnlI CCTC 7 cut(s) 113, 249, 255, 301, 505, 549, 575
MroXI GAANNNNTTC 1 cut(s) 322
MseI TTAA 2 cut(s) 654, 669
MspR9I CCNGG 1 cut(s) 80
Mva1269I GAATGC 1 cut(s) 317
MvaI CCWGG 1 cut(s) 80
MwoI GCNNNNNNNGC 2 cut(s) 353, 791
NdeI CATATG 1 cut(s) 217
NdeII GATC 2 cut(s) 185, 657
NlaIII CATG 2 cut(s) 428, 811
NlaIV GGNNCC 1 cut(s) 177
NmuCI GTSAC 1 cut(s) 673
NspV TTCGAA 2 cut(s) 168, 630
PceI AGGCCT 2 cut(s) 240, 301
PcsI WCGNNNNNNNCGW 1 cut(s) 190
PctI GAATGC 1 cut(s) 317
PdmI GAANNNNTTC 1 cut(s) 322
PfeI GAWTC 2 cut(s) 72, 391
PkrI GCNGC 5 cut(s) 10, 346, 358, 366, 736
PleI GAGTC 3 cut(s) 384, 466, 488
PpsI GAGTC 3 cut(s) 384, 466, 488
Psp6I CCWGG 1 cut(s) 78
PspFI CCCAGC 1 cut(s) 126
PspGI CCWGG 1 cut(s) 78
PspN4I GGNNCC 1 cut(s) 177
PstNI CAGNNNCTG 1 cut(s) 113
PsuI RGATCY 1 cut(s) 657
RsaI GTAC 1 cut(s) 858
RsaNI GTAC 1 cut(s) 857
SaqAI TTAA 2 cut(s) 654, 669
SatI GCNGC 5 cut(s) 9, 345, 357, 365, 735
Sau3AI GATC 2 cut(s) 185, 657
SchI GAGTC 3 cut(s) 385, 466, 488
ScrFI CCNGG 1 cut(s) 80
SduI GDGCHC 1 cut(s) 842
SfaNI GCATC 2 cut(s) 526, 607
SfcI CTRYAG 1 cut(s) 430
SfuI TTCGAA 2 cut(s) 168, 630
Sse9I AATT 3 cut(s) 207, 460, 632
SseBI AGGCCT 2 cut(s) 240, 301
SsiI CCGC 2 cut(s) 9, 364
SspMI CTAG 2 cut(s) 249, 509
StuI AGGCCT 2 cut(s) 240, 301
StyD4I CCNGG 1 cut(s) 78
TaqI TCGA 4 cut(s) 168, 410, 630, 804
TaqII GACCGA 1 cut(s) 408
TasI AATT 3 cut(s) 207, 460, 632
TatI WGTACW 1 cut(s) 856
TauI GCSGC 2 cut(s) 11, 367
TfiI GAWTC 2 cut(s) 72, 391
Tru1I TTAA 2 cut(s) 654, 669
Tru9I TTAA 2 cut(s) 654, 669
TscAI CASTG 2 cut(s) 166, 796
TseFI GTSAC 1 cut(s) 673
TseI GCWGC 3 cut(s) 344, 356, 734
Tsp45I GTSAC 1 cut(s) 673
TspDTI ATGAA 5 cut(s) 261, 267, 345, 531, 824
TspRI CASTG 2 cut(s) 166, 796
XmnI GAANNNNTTC 1 cut(s) 322
XspI CTAG 2 cut(s) 249, 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.