Rh4CG352000

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0007892
Physical Location & Seq
Reverse (-)
60878759 .. 60882180
3422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG352000.1

Sequence Viewer

Length: 465 bp
ATGAGGGATCTGGAAAACCAAAAGCCACATGGCCTGATGAGTAAGGCTACAAATCTAAGTGAAGAGTTTCGTATCAATCCAGAGTATCGCAAATTTCGTGATGTTGGAATTAGTCCTGAAATGATGGCTGTTTATGATAACATGTTTAGGGGTAGCACAGCCCTAGGTCACTTTGTCATGATTCCCTCAGCTTCTATAAATATTGAAGAGGTGGTGGAGGATTCTGAGCATGATATCTACATAGTTAAGGTGACAAATCAAGAACATTGCAAGAAGGACAAAGTGAAGAGTGCTGGAAAGCCATCAAAGGCAGCCAAGAAGCCTGACCAAGACACTAACAAAAAACAAAGGTCAAAGAAAGCTACCACTGCTGAAATAATCGACATGAAAACACCCCCAGAGCTTCTACCAGAGAGCCCAAAAAGTGAAGTTGTACTACCATATAGACTCAGGAAGAGAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

154

Amino Acids

17.56

Weight (kDa)

9.23

Isoelectric Point (pI)

43.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 15
AcsI RAATTY 1 cut(s) 92
AfaI GTAC 1 cut(s) 435
AflIII ACRYGT 1 cut(s) 141
AgsI TTSAA 1 cut(s) 206
AluBI AGCT 3 cut(s) 191, 362, 403
AluI AGCT 3 cut(s) 191, 362, 403
AlwI GGATC 1 cut(s) 15
AoxI GGCC 1 cut(s) 31
ApeKI GCWGC 1 cut(s) 311
ApoI RAATTY 1 cut(s) 92
Asp700I GAANNNNTTC 1 cut(s) 66
AspA2I CCTAGG 1 cut(s) 163
AsuHPI GGTGA 1 cut(s) 262
AvrII CCTAGG 1 cut(s) 163
BanII GRGCYC 1 cut(s) 419
BarI GAAGNNNNNNTAC 2 cut(s) 420, 452
BbvCI CCTCAGC 1 cut(s) 187
BbvI GCAGC 1 cut(s) 323
BccI CCATC 2 cut(s) 118, 310
BfaI CTAG 1 cut(s) 164
BisI GCNGC 1 cut(s) 312
BlnI CCTAGG 1 cut(s) 163
BlsI GCNGC 1 cut(s) 313
Bpu10I CCTNAGC 1 cut(s) 187
BsaJI CCNNGG 1 cut(s) 163
Bse3DI GCAATG 1 cut(s) 265
BseDI CCNNGG 1 cut(s) 163
BseMI GCAATG 1 cut(s) 265
BseMII CTCAG 3 cut(s) 201, 216, 463
BseXI GCAGC 1 cut(s) 323
BshFI GGCC 1 cut(s) 33
BsnI GGCC 1 cut(s) 33
Bsp1286I GDGCHC 1 cut(s) 419
Bsp143I GATC 1 cut(s) 7
BspANI GGCC 1 cut(s) 33
BspCNI CTCAG 3 cut(s) 200, 217, 462
BspHI TCATGA 1 cut(s) 177
BspPI GGATC 1 cut(s) 15
BsrDI GCAATG 1 cut(s) 265
BssECI CCNNGG 1 cut(s) 163
BssMI GATC 1 cut(s) 7
BssT1I CCWWGG 1 cut(s) 163
Bst6I CTCTTC 4 cut(s) 57, 201, 281, 449
BstDEI CTNAG 4 cut(s) 56, 187, 225, 449
BstKTI GATC 1 cut(s) 10
BstMBI GATC 1 cut(s) 7
BstMWI GCNNNNNNNGC 1 cut(s) 368
BstNSI RCATGY 1 cut(s) 145
BstV1I GCAGC 1 cut(s) 323
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
BsuRI GGCC 1 cut(s) 33
BtsI GCAGTG 1 cut(s) 366
BtsIMutI CAGTG 1 cut(s) 366
CciI TCATGA 1 cut(s) 177
Csp6I GTAC 1 cut(s) 434
CviAII CATG 5 cut(s) 29, 142, 178, 230, 385
CviQI GTAC 1 cut(s) 434
DdeI CTNAG 4 cut(s) 56, 187, 225, 449
DpnI GATC 1 cut(s) 9
DpnII GATC 1 cut(s) 7
Eam1104I CTCTTC 4 cut(s) 57, 201, 281, 449
EarI CTCTTC 4 cut(s) 57, 201, 281, 449
Eco130I CCWWGG 1 cut(s) 163
Eco24I GRGCYC 1 cut(s) 419
Eco32I GATATC 1 cut(s) 235
EcoRV GATATC 1 cut(s) 235
EcoT14I CCWWGG 1 cut(s) 163
EcoT38I GRGCYC 1 cut(s) 419
ErhI CCWWGG 1 cut(s) 163
FaeI CATG 5 cut(s) 32, 145, 181, 233, 388
FatI CATG 5 cut(s) 28, 141, 177, 229, 384
Fnu4HI GCNGC 1 cut(s) 312
FriOI GRGCYC 1 cut(s) 419
Fsp4HI GCNGC 1 cut(s) 312
FspBI CTAG 1 cut(s) 164
GluI GCNGC 1 cut(s) 312
HaeIII GGCC 1 cut(s) 33
Hin1II CATG 5 cut(s) 32, 145, 181, 233, 388
HinfI GANTC 3 cut(s) 181, 221, 447
HphI GGTGA 1 cut(s) 262
Hpy188I TCNGA 1 cut(s) 226
Hpy188III TCNNGA 7 cut(s) 11, 80, 98, 116, 178, 260, 451
HpyAV CCTTC 1 cut(s) 268
HpyCH4V TGCA 1 cut(s) 270
HpyF10VI GCNNNNNNNGC 1 cut(s) 368
HpyF3I CTNAG 4 cut(s) 56, 187, 225, 449
Hsp92II CATG 5 cut(s) 32, 145, 181, 233, 388
Kzo9I GATC 1 cut(s) 7
LpnPI CCDG 8 cut(s) 47, 93, 129, 279, 336, 411, 423, 436
Lsp1109I GCAGC 1 cut(s) 323
MaeI CTAG 1 cut(s) 164
MaeIII GTNAC 2 cut(s) 167, 250
MalI GATC 1 cut(s) 9
MboI GATC 1 cut(s) 7
MboII GAAGA 3 cut(s) 74, 218, 298
MflI RGATCY 1 cut(s) 7
MhlI GDGCHC 1 cut(s) 419
MluCI AATT 2 cut(s) 92, 108
MlyI GAGTC 1 cut(s) 441
MmeI TCCRAC 1 cut(s) 85
MnlI CCTC 3 cut(s) 196, 202, 211
MroXI GAANNNNTTC 1 cut(s) 66
MseI TTAA 1 cut(s) 246
MwoI GCNNNNNNNGC 1 cut(s) 368
NdeII GATC 1 cut(s) 7
NlaIII CATG 5 cut(s) 32, 145, 181, 233, 388
NmuCI GTSAC 2 cut(s) 167, 250
NspI RCATGY 1 cut(s) 145
PagI TCATGA 1 cut(s) 177
PciI ACATGT 1 cut(s) 141
PcsI WCGNNNNNNNCGW 1 cut(s) 94
PdmI GAANNNNTTC 1 cut(s) 66
PfeI GAWTC 2 cut(s) 181, 221
PkrI GCNGC 1 cut(s) 313
PleI GAGTC 1 cut(s) 441
PpsI GAGTC 1 cut(s) 441
PscI ACATGT 1 cut(s) 141
PsuI RGATCY 1 cut(s) 7
RsaI GTAC 1 cut(s) 435
RsaNI GTAC 1 cut(s) 434
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 1 cut(s) 312
Sau3AI GATC 1 cut(s) 7
SchI GAGTC 1 cut(s) 441
SduI GDGCHC 1 cut(s) 419
SetI ASST 7 cut(s) 169, 193, 213, 252, 353, 364, 405
Sse9I AATT 2 cut(s) 92, 108
SspI AATATT 1 cut(s) 202
SspMI CTAG 1 cut(s) 164
StyI CCWWGG 1 cut(s) 163
TaqI TCGA 1 cut(s) 381
TasI AATT 2 cut(s) 92, 108
TatI WGTACW 1 cut(s) 433
TfiI GAWTC 2 cut(s) 181, 221
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TscAI CASTG 1 cut(s) 373
TseFI GTSAC 2 cut(s) 167, 250
TseI GCWGC 1 cut(s) 311
Tsp45I GTSAC 2 cut(s) 167, 250
TspDTI ATGAA 1 cut(s) 401
TspRI CASTG 1 cut(s) 373
XapI RAATTY 1 cut(s) 92
XceI RCATGY 1 cut(s) 145
XcmI CCANNNNNNNNNTGG 1 cut(s) 26
XmaJI CCTAGG 1 cut(s) 163
XmnI GAANNNNTTC 1 cut(s) 66
XspI CTAG 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.