Rh3AG173000

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0012704
Physical Location & Seq
Forward (+)
16046082 .. 16046297
216 bp
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UTR
Exon/CDS
Intron
Rh3AG173000.1

Sequence Viewer

Length: 216 bp
ATGGTGCAGCTCGACTCGCACCCGAAGGTTCTAGCCAAGGTCCGGGAAGAGGTCGCTCGAGTTTTGGACCCAGAGTCGGATGAACTGATCATGGCAGACCAACTGGCGCGGATGAGGTACACGCACGCCGTGGCGCGTGAAGTCGTGAGGTACCACTCTCCTGCCACCATGGTCCCTCACTTTGCGGCTATTGACTTTTCATTTTTTGAGACGTGA

Protein Analysis

71

Amino Acids

8.17

Weight (kDa)

5.7

Isoelectric Point (pI)

26.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 3 - 68 2.1e-10 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 150
AccB1I GGYRCC 1 cut(s) 150
AccII CGCG 2 cut(s) 109, 136
AciI CCGC 2 cut(s) 109, 185
AdeI CACNNNGTG 1 cut(s) 130
AfaI GTAC 2 cut(s) 119, 152
AfiI CCNNNNNNNGG 3 cut(s) 42, 49, 76
AhdI GACNNNNNGTC 1 cut(s) 73
AjiI CACGTC 1 cut(s) 213
AluBI AGCT 1 cut(s) 10
AluI AGCT 1 cut(s) 10
Alw26I GTCTC 1 cut(s) 203
Ama87I CYCGRG 1 cut(s) 57
ApeKI GCWGC 1 cut(s) 7
Asp718I GGTACC 1 cut(s) 150
AspLEI GCGC 2 cut(s) 109, 136
AspS9I GGNCC 3 cut(s) 40, 67, 172
AsuC2I CCSGG 1 cut(s) 44
AvaI CYCGRG 1 cut(s) 57
AvaII GGWCC 3 cut(s) 40, 67, 172
BanI GGYRCC 1 cut(s) 150
BbvI GCAGC 1 cut(s) 19
BceAI ACGGC 1 cut(s) 113
BclI TGATCA 1 cut(s) 87
BcnI CCSGG 1 cut(s) 44
BcoDI GTCTC 1 cut(s) 203
BfaI CTAG 1 cut(s) 32
BisI GCNGC 2 cut(s) 8, 186
BlsI GCNGC 2 cut(s) 9, 187
Bme1390I CCNGG 1 cut(s) 44
Bme18I GGWCC 3 cut(s) 40, 67, 172
BmeRI GACNNNNNGTC 1 cut(s) 73
BmeT110I CYCGRG 1 cut(s) 57
BmgBI CACGTC 1 cut(s) 213
BmgT120I GGNCC 3 cut(s) 40, 67, 172
BmiI GGNNCC 3 cut(s) 69, 152, 174
BmrFI CCNGG 1 cut(s) 44
BpuMI CCSGG 1 cut(s) 44
BsaJI CCNNGG 3 cut(s) 36, 129, 168
Bsc4I CCNNNNNNNGG 3 cut(s) 42, 49, 76
Bse1I ACTGG 1 cut(s) 108
BseDI CCNNGG 3 cut(s) 36, 129, 168
BseGI GGATG 2 cut(s) 85, 117
BseLI CCNNNNNNNGG 3 cut(s) 42, 49, 76
BseNI ACTGG 1 cut(s) 108
BseXI GCAGC 1 cut(s) 19
BsgI GTGCAG 1 cut(s) 26
Bsh1236I CGCG 2 cut(s) 109, 136
BshNI GGYRCC 1 cut(s) 150
BsiHKCI CYCGRG 1 cut(s) 57
BsiSI CCGG 1 cut(s) 43
BslFI GGGAC 1 cut(s) 158
BslI CCNNNNNNNGG 3 cut(s) 42, 49, 76
BsmAI GTCTC 1 cut(s) 203
BsmBI CGTCTC 1 cut(s) 203
BsmFI GGGAC 1 cut(s) 158
BsoBI CYCGRG 1 cut(s) 57
Bsp143I GATC 1 cut(s) 87
Bsp19I CCATGG 1 cut(s) 168
BspACI CCGC 2 cut(s) 109, 185
BspFNI CGCG 2 cut(s) 109, 136
BspLI GGNNCC 3 cut(s) 69, 152, 174
BspT107I GGYRCC 1 cut(s) 150
BsrI ACTGG 1 cut(s) 108
BssECI CCNNGG 3 cut(s) 36, 129, 168
BssMI GATC 1 cut(s) 87
BssT1I CCWWGG 2 cut(s) 36, 168
Bst6I CTCTTC 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 126
BstDSI CCRYGG 2 cut(s) 129, 168
BstF5I GGATG 2 cut(s) 85, 117
BstFNI CGCG 2 cut(s) 109, 136
BstHHI GCGC 2 cut(s) 109, 136
BstKTI GATC 1 cut(s) 90
BstMAI GTCTC 1 cut(s) 203
BstMBI GATC 1 cut(s) 87
BstMWI GCNNNNNNNGC 1 cut(s) 16
BstSCI CCNGG 1 cut(s) 42
BstUI CGCG 2 cut(s) 109, 136
BstV1I GCAGC 1 cut(s) 19
BtgI CCRYGG 2 cut(s) 129, 168
BtrI CACGTC 1 cut(s) 213
BtsCI GGATG 2 cut(s) 85, 117
Cac8I GCNNGC 1 cut(s) 126
CfoI GCGC 2 cut(s) 109, 136
Cfr13I GGNCC 3 cut(s) 40, 67, 172
Csp6I GTAC 2 cut(s) 118, 151
CviAII CATG 2 cut(s) 91, 169
CviJI RGCY 3 cut(s) 10, 35, 188
CviKI_1 RGCY 3 cut(s) 10, 35, 188
CviQI GTAC 2 cut(s) 118, 151
DpnI GATC 1 cut(s) 89
DpnII GATC 1 cut(s) 87
DraIII CACNNNGTG 1 cut(s) 130
DriI GACNNNNNGTC 1 cut(s) 73
Eam1104I CTCTTC 1 cut(s) 42
Eam1105I GACNNNNNGTC 1 cut(s) 73
EarI CTCTTC 1 cut(s) 42
Eco130I CCWWGG 2 cut(s) 36, 168
Eco47I GGWCC 3 cut(s) 40, 67, 172
Eco88I CYCGRG 1 cut(s) 57
EcoT14I CCWWGG 2 cut(s) 36, 168
ErhI CCWWGG 2 cut(s) 36, 168
Esp3I CGTCTC 1 cut(s) 203
FaeI CATG 2 cut(s) 94, 172
FaiI YATR 2 cut(s) 92, 170
FaqI GGGAC 1 cut(s) 158
FatI CATG 2 cut(s) 90, 168
FbaI TGATCA 1 cut(s) 87
Fnu4HI GCNGC 2 cut(s) 8, 186
FokI GGATG 2 cut(s) 92, 124
Fsp4HI GCNGC 2 cut(s) 8, 186
FspBI CTAG 1 cut(s) 32
GlaI GCGC 2 cut(s) 108, 135
GluI GCNGC 2 cut(s) 8, 186
HapII CCGG 1 cut(s) 43
HhaI GCGC 2 cut(s) 109, 136
Hin1II CATG 2 cut(s) 94, 172
Hin6I GCGC 2 cut(s) 107, 134
HinP1I GCGC 2 cut(s) 107, 134
HinfI GANTC 2 cut(s) 14, 74
HpaII CCGG 1 cut(s) 43
Hpy166II GTNNAC 1 cut(s) 120
Hpy188I TCNGA 1 cut(s) 79
Hpy188III TCNNGA 1 cut(s) 145
Hpy8I GTNNAC 1 cut(s) 120
HpyAV CCTTC 1 cut(s) 19
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 1 cut(s) 7
HpyF10VI GCNNNNNNNGC 1 cut(s) 16
HpySE526I ACGT 1 cut(s) 212
Hsp92II CATG 2 cut(s) 94, 172
HspAI GCGC 2 cut(s) 107, 134
KpnI GGTACC 1 cut(s) 154
Ksp22I TGATCA 1 cut(s) 87
Kzo9I GATC 1 cut(s) 87
LpnPI CCDG 4 cut(s) 56, 84, 89, 174
Lsp1109I GCAGC 1 cut(s) 19
MaeI CTAG 1 cut(s) 32
MaeII ACGT 1 cut(s) 212
MalI GATC 1 cut(s) 89
MboI GATC 1 cut(s) 87
MboII GAAGA 1 cut(s) 59
MlyI GAGTC 2 cut(s) 8, 83
MmeI TCCRAC 1 cut(s) 57
MnlI CCTC 4 cut(s) 43, 108, 141, 186
MspI CCGG 1 cut(s) 43
MspR9I CCNGG 1 cut(s) 44
MvnI CGCG 2 cut(s) 109, 136
MwoI GCNNNNNNNGC 1 cut(s) 16
NciI CCSGG 1 cut(s) 44
NcoI CCATGG 1 cut(s) 168
NdeII GATC 1 cut(s) 87
NlaIII CATG 2 cut(s) 94, 172
NlaIV GGNNCC 3 cut(s) 69, 152, 174
PaeR7I CTCGAG 1 cut(s) 57
PfoI TCCNGGA 1 cut(s) 42
PkrI GCNGC 2 cut(s) 9, 187
PleI GAGTC 2 cut(s) 8, 82
PpsI GAGTC 2 cut(s) 8, 82
PspN4I GGNNCC 3 cut(s) 69, 152, 174
PspPI GGNCC 3 cut(s) 40, 67, 172
PspXI VCTCGAGB 1 cut(s) 57
RsaI GTAC 2 cut(s) 119, 152
RsaNI GTAC 2 cut(s) 118, 151
SatI GCNGC 2 cut(s) 8, 186
Sau3AI GATC 1 cut(s) 87
Sau96I GGNCC 3 cut(s) 40, 67, 172
SchI GAGTC 2 cut(s) 8, 83
ScrFI CCNGG 1 cut(s) 44
SetI ASST 7 cut(s) 12, 30, 42, 54, 119, 152, 215
Sfr274I CTCGAG 1 cut(s) 57
SinI GGWCC 3 cut(s) 40, 67, 172
SlaI CTCGAG 1 cut(s) 57
SmlI CTYRAG 1 cut(s) 57
SmoI CTYRAG 1 cut(s) 57
SsiI CCGC 2 cut(s) 109, 185
SspMI CTAG 1 cut(s) 32
StyD4I CCNGG 1 cut(s) 42
StyI CCWWGG 2 cut(s) 36, 168
TaiI ACGT 1 cut(s) 215
TaqI TCGA 2 cut(s) 12, 58
TauI GCSGC 1 cut(s) 188
TseI GCWGC 1 cut(s) 7
TspDTI ATGAA 2 cut(s) 96, 189
VpaK11BI GGWCC 3 cut(s) 40, 67, 172
XhoI CTCGAG 1 cut(s) 57
XspI CTAG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.