Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0004605

Physical Location & Seq

Genomic Coordinates Forward (+)

8683679 .. 8686436

Gene Structure 2758 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1064500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1332 bp

ATGGGATTCCTTTTTCAAGCATTATATGAAGCTGAATATTGTTCAGATTCCCCTTTTCCGAAGCCAAGCTCGATTCACTGGAGAACCACTGTTCCAACAAGACGGTCAGCGAAAAGCTGGACAGCGGAAGAGGATAATCTTCTAGGAGAGTTAGTCACAAAGTTCAACAGGAGCAGTTGGAAGAAAATAGCTGCATGTCTCCCCGGGCGTACAGATATTCAGTGCCTGCAACGCTGGAAAAAGGTTTTAAATCCAGAAGTCGTCAAGGGAGCCTGGACAAAGGAGGAAGATGATTGCATTATCAAGCAAGTCGAAAAACATGGTGCCAAAAGATGGTCTGTTATAGCTAAGTTCCTACCAGGTCGAATAGGCAAACAATGCCGAGAAAGGTGGCACAATCACTTATGCCCGGCAATTAATAGAGATGCATGGACAAAAGACGAGGAGCAGATTATTGCTTACTACCATCAGTTGTACGGTAACAAGTGGGCAGAAATAGCAAGATTTCTACCCGGGAGGACCGACAATGCAATTAAAAATTTCTGGAATTGCACGTTGAAGAGGAAATTCGATTCATACTCACCTAATGGATGTGAAGTGGATATGCAATCTGATGTCTCAAGTGGTGAGGTAAGAACAGATCGTCTTGAGGTTGAAGAAGATGGACAGATTTCTGACAGTGTGGTCTCACTCAATCAGCTAACGGAAACGGAGATTAGTGACAATGCTTGTTCTACTGAGTTGACTCTTGGATTTTCTTACAGTCATGACATTTGTTCAGAATGGAAATCTGTCAAAGTGCAGAAGCGCAGGTCTACGGAGAGACGAGCAAGCAGTTTAATAAATCCACAAATTGCCACTCCATTTGCTTTCATGGACGCATCCCCTTGTACCAAATCGAACTGCATTCCTCCTACTAGGCCACCTTGCTATTCGACTCCCCGAAGCCTTGCGCAGAACACCTCTGTTAGCAGTGGCAGTCCGGGGTCTAGATTGAAGAACTTAGCATTGAGTTTCAAAAACACTCCTTCCATCATAAGAAAGAAAAGCTCCAGAAGGAGTGATCCTAATAACTTCCCTGATGTCAACAGCTCAAAGGCATGCTCCACGTTGAGTAGTACTAGTGAAGGAGGAGGTACCAACAGCAGAGACTTTGCAAACGGGAAGTTTGGTTCATTTTCTTTCCGTCATAGGCCGCAAAATTCAGCTGCTGTTAAATCTCTAGGACGGCGCCTAGAACACGAATTCGATGCCGAAAAGGATTCGGGTAGTGATCAATGTGGCCATGCATTCTCTGCATATGCGTCTCCAGATGTCGGTTTTGGTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0008285 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010564 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032465 GO:0032502 GO:0042127 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051302 GO:0051726 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901181 GO:1901363 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:2000112 GO:2000113 GO:2001023 GO:2001024 GO:2001038 GO:2001039 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04151 ko05166 map04151 map05166

Pfam Domains

Protein Families

Myb_DNA-binding

Protein Analysis

444

Amino Acids

49.63

Weight (kDa)

8.93

Isoelectric Point (pI)

56.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	38 - 83	6.4e-13	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	40 - 97	4.7e-15	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	89 - 135	5.1e-19	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	92 - 150	2e-17	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	141 - 183	1.5e-15	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	144 - 190	3.4e-10	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004605)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G13960 AT2G13960 AT2G13960 AT3G09370 AT3G09370 AT3G09370
fragaria_vesca	FvH4_5g01790
malus_domestica	MD04G1064500.v1.1 MD08G1117700.v1.1
prunus_persica	Prupe.5G093400_v2.0.a1 Prupe.5G093400_v2.0.a1
pyrus_communis	pycom04g05830
rosa_chinensis	RchiOBHm_Chr7g0198881
rosa_laevigata	RLG00000003835 RLG00000003838
rosa_multiflora	Rmu_sc0016854.1_g000006
rosa_roxburghii	Rroxscaffold_3G00257330 Rroxscaffold_3G00257350
rosa_rugosa	Rorug07G0050200 Rorug07G0050300 Rorug07G0050400
rosa_samantha	Rh4BG081200 Rh4DG076200 Rh7AG173800 Rh7AG174200 Rh7BG177200 Rh7CG183300 Rh7DG176500 Rh7DG176700
rosa_wichuraiana	Rw7G015220 Rw7G015250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	683
Acc16I	TGCGCA	1 cut(s)	954
Acc36I	ACCTGC	1 cut(s)	801
Acc65I	GGTACC	1 cut(s)	1136
AccB1I	GGYRCC	3 cut(s)	323, 1136, 1230
AccB7I	CCANNNNNTGG	1 cut(s)	333
AccI	GTMKAC	1 cut(s)	815
AciI	CCGC	2 cut(s)	125, 1196
AclWI	GGATC	1 cut(s)	1058
AcoI	YGGCCR	1 cut(s)	1282
AcsI	RAATTY	4 cut(s)	538, 566, 1201, 1244
AcyI	GRCGYC	1 cut(s)	1231
AfaI	GTAC	5 cut(s)	211, 476, 892, 1120, 1138
AfiI	CCNNNNNNNGG	2 cut(s)	333, 1316
AgsI	TTSAA	6 cut(s)	17, 166, 559, 656, 997, 1018
AhlI	ACTAGT	1 cut(s)	1121
AjnI	CCWGG	2 cut(s)	272, 358
AloI	GAACNNNNNNTCC	4 cut(s)	76, 108, 1156, 1188
AluBI	AGCT	9 cut(s)	32, 69, 117, 191, 347, 700, 1050, 1092, 1208
AluI	AGCT	9 cut(s)	32, 69, 117, 191, 347, 700, 1050, 1092, 1208
Alw26I	GTCTC	6 cut(s)	203, 622, 691, 817, 1143, 1311
AlwI	GGATC	1 cut(s)	1058
AlwNI	CAGNNNCTG	2 cut(s)	226, 1211
Ama87I	CYCGRG	2 cut(s)	203, 512
AoxI	GGCC	3 cut(s)	920, 1193, 1282
ApeKI	GCWGC	2 cut(s)	191, 1208
ApoI	RAATTY	4 cut(s)	538, 566, 1201, 1244
AseI	ATTAAT	1 cut(s)	417
Asp718I	GGTACC	1 cut(s)	1136
AspLEI	GCGC	3 cut(s)	810, 955, 1233
AspS9I	GGNCC	1 cut(s)	519
AsuC2I	CCSGG	6 cut(s)	204, 205, 410, 513, 514, 984
AsuHPI	GGTGA	2 cut(s)	573, 638
AvaI	CYCGRG	2 cut(s)	203, 512
AvaII	GGWCC	1 cut(s)	519
BalI	TGGCCA	1 cut(s)	1284
BanI	GGYRCC	3 cut(s)	323, 1136, 1230
BbvI	GCAGC	2 cut(s)	178, 1195
BccI	CCATC	4 cut(s)	327, 474, 656, 1040
BceAI	ACGGC	1 cut(s)	1244
BcgI	CGANNNNNNTGC	2 cut(s)	1232, 1266
BciT130I	CCWGG	2 cut(s)	274, 360
BclI	TGATCA	1 cut(s)	1273
BcnI	CCSGG	6 cut(s)	204, 205, 410, 513, 514, 984
BcoDI	GTCTC	6 cut(s)	203, 622, 691, 817, 1143, 1311
BcuI	ACTAGT	1 cut(s)	1121
BfaI	CTAG	6 cut(s)	143, 918, 990, 1122, 1223, 1235
BfoI	RGCGCY	1 cut(s)	1234
BfuAI	ACCTGC	1 cut(s)	801
BisI	GCNGC	3 cut(s)	192, 1196, 1209
BlsI	GCNGC	3 cut(s)	193, 1197, 1210
BmcAI	AGTACT	1 cut(s)	1120
Bme1390I	CCNGG	8 cut(s)	204, 205, 274, 360, 410, 513, 514, 984
Bme18I	GGWCC	1 cut(s)	519
BmeT110I	CYCGRG	2 cut(s)	203, 512
BmgT120I	GGNCC	1 cut(s)	519
BmiI	GGNNCC	4 cut(s)	271, 325, 1138, 1232
BmrFI	CCNGG	8 cut(s)	204, 205, 274, 360, 410, 513, 514, 984
BmsI	GCATC	3 cut(s)	415, 890, 1240
BpmI	CTGGAG	3 cut(s)	100, 1036, 1293
BpuEI	CTTGAG	2 cut(s)	604, 668
BpuMI	CCSGG	6 cut(s)	204, 205, 410, 513, 514, 984
BsaHI	GRCGYC	1 cut(s)	1231
BsaI	GGTCTC	1 cut(s)	691
BsaJI	CCNNGG	4 cut(s)	202, 203, 512, 983
BsaXI	ACNNNNNCTCC	2 cut(s)	138, 168
Bsc4I	CCNNNNNNNGG	2 cut(s)	333, 1316
Bse1I	ACTGG	1 cut(s)	83
BseBI	CCWGG	2 cut(s)	274, 360
BseDI	CCNNGG	4 cut(s)	202, 203, 512, 983
BseGI	GGATG	2 cut(s)	596, 881
BseLI	CCNNNNNNNGG	2 cut(s)	333, 1316
BseMII	CTCAG	1 cut(s)	729
BseNI	ACTGG	1 cut(s)	83
BseRI	GAGGAG	2 cut(s)	458, 1146
BseXI	GCAGC	2 cut(s)	178, 1195
BsgI	GTGCAG	1 cut(s)	821
BshFI	GGCC	3 cut(s)	922, 1195, 1284
BshNI	GGYRCC	3 cut(s)	323, 1136, 1230
BsiHKCI	CYCGRG	2 cut(s)	203, 512
BsiSI	CCGG	4 cut(s)	204, 410, 513, 983
BslI	CCNNNNNNNGG	2 cut(s)	333, 1316
BsmAI	GTCTC	6 cut(s)	203, 622, 691, 817, 1143, 1311
BsmBI	CGTCTC	2 cut(s)	817, 1311
BsmI	GAATGC	2 cut(s)	906, 1289
BsnI	GGCC	3 cut(s)	922, 1195, 1284
Bso31I	GGTCTC	1 cut(s)	691
BsoBI	CYCGRG	2 cut(s)	203, 512
Bsp143I	GATC	3 cut(s)	640, 1063, 1273
BspACI	CCGC	2 cut(s)	125, 1196
BspANI	GGCC	3 cut(s)	922, 1195, 1284
BspCNI	CTCAG	1 cut(s)	730
BspHI	TCATGA	1 cut(s)	766
BspLI	GGNNCC	4 cut(s)	271, 325, 1138, 1232
BspMI	ACCTGC	1 cut(s)	801
BspPI	GGATC	1 cut(s)	1058
BspT107I	GGYRCC	3 cut(s)	323, 1136, 1230
BspTNI	GGTCTC	1 cut(s)	691
BsrI	ACTGG	1 cut(s)	83
BssECI	CCNNGG	4 cut(s)	202, 203, 512, 983
BssMI	GATC	3 cut(s)	640, 1063, 1273
BssNI	GRCGYC	1 cut(s)	1231
Bst2UI	CCWGG	2 cut(s)	274, 360
Bst4CI	ACNGT	5 cut(s)	91, 105, 479, 680, 764
Bst6I	CTCTTC	2 cut(s)	123, 554
BstACI	GRCGYC	1 cut(s)	1231
BstAPI	GCANNNNNTGC	2 cut(s)	378, 1295
BstC8I	GCNNGC	3 cut(s)	227, 832, 1102
BstDEI	CTNAG	3 cut(s)	348, 738, 1003
BstF5I	GGATG	2 cut(s)	596, 881
BstH2I	RGCGCY	1 cut(s)	1234
BstHHI	GCGC	3 cut(s)	810, 955, 1233
BstKTI	GATC	3 cut(s)	643, 1066, 1276
BstMAI	GTCTC	6 cut(s)	203, 622, 691, 817, 1143, 1311
BstMBI	GATC	3 cut(s)	640, 1063, 1273
BstMWI	GCNNNNNNNGC	4 cut(s)	231, 378, 497, 1295
BstNI	CCWGG	2 cut(s)	274, 360
BstNSI	RCATGY	2 cut(s)	198, 1104
BstSCI	CCNGG	8 cut(s)	202, 203, 272, 358, 408, 511, 512, 982
BstV1I	GCAGC	2 cut(s)	178, 1195
BsuRI	GGCC	3 cut(s)	922, 1195, 1284
BtsCI	GGATG	2 cut(s)	596, 881
BtsI	GCAGTG	1 cut(s)	979
BtsIMutI	CAGTG	5 cut(s)	76, 87, 227, 685, 979
BveI	ACCTGC	1 cut(s)	801
Cac8I	GCNNGC	3 cut(s)	227, 832, 1102
CaiI	CAGNNNCTG	2 cut(s)	226, 1211
CciI	TCATGA	1 cut(s)	766
CfoI	GCGC	3 cut(s)	810, 955, 1233
Cfr13I	GGNCC	1 cut(s)	519
Cfr9I	CCCGGG	2 cut(s)	203, 512
CseI	GACGC	2 cut(s)	887, 1293
CsiI	ACCWGGT	1 cut(s)	358
Csp6I	GTAC	5 cut(s)	210, 475, 891, 1119, 1137
CspCI	CAANNNNNGTGG	2 cut(s)	847, 882
CviAII	CATG	7 cut(s)	195, 320, 429, 767, 874, 1101, 1286
CviQI	GTAC	5 cut(s)	210, 475, 891, 1119, 1137
DdeI	CTNAG	3 cut(s)	348, 738, 1003
DinI	GGCGCC	1 cut(s)	1232
DpnI	GATC	3 cut(s)	642, 1065, 1275
DpnII	GATC	3 cut(s)	640, 1063, 1273
DraI	TTTAAA	1 cut(s)	249
DrdI	GACNNNNNNGTC	1 cut(s)	683
DseDI	GACNNNNNNGTC	1 cut(s)	683
EaeI	YGGCCR	1 cut(s)	1282
Eam1104I	CTCTTC	2 cut(s)	123, 554
EarI	CTCTTC	2 cut(s)	123, 554
Eco31I	GGTCTC	1 cut(s)	691
Eco47I	GGWCC	1 cut(s)	519
Eco88I	CYCGRG	2 cut(s)	203, 512
EcoRI	GAATTC	1 cut(s)	1244
EcoRII	CCWGG	2 cut(s)	272, 358
EcoT22I	ATGCAT	2 cut(s)	430, 1291
EgeI	GGCGCC	1 cut(s)	1232
EheI	GGCGCC	1 cut(s)	1232
Esp3I	CGTCTC	2 cut(s)	817, 1311
FaeI	CATG	7 cut(s)	198, 323, 432, 770, 877, 1104, 1289
FatI	CATG	7 cut(s)	194, 319, 428, 766, 873, 1100, 1285
FauNDI	CATATG	1 cut(s)	1300
FbaI	TGATCA	1 cut(s)	1273
FblI	GTMKAC	1 cut(s)	815
Fnu4HI	GCNGC	3 cut(s)	192, 1196, 1209
FokI	GGATG	2 cut(s)	603, 868
Fsp4HI	GCNGC	3 cut(s)	192, 1196, 1209
FspBI	CTAG	6 cut(s)	143, 918, 990, 1122, 1223, 1235
FspI	TGCGCA	1 cut(s)	954
GlaI	GCGC	3 cut(s)	809, 954, 1232
GluI	GCNGC	3 cut(s)	192, 1196, 1209
GsuI	CTGGAG	3 cut(s)	100, 1036, 1293
HaeII	RGCGCY	1 cut(s)	1234
HaeIII	GGCC	3 cut(s)	922, 1195, 1284
HapII	CCGG	4 cut(s)	204, 410, 513, 983
HgaI	GACGC	2 cut(s)	887, 1293
HhaI	GCGC	3 cut(s)	810, 955, 1233
Hin1I	GRCGYC	1 cut(s)	1231
Hin1II	CATG	7 cut(s)	198, 323, 432, 770, 877, 1104, 1289
Hin6I	GCGC	3 cut(s)	808, 953, 1231
HinP1I	GCGC	3 cut(s)	808, 953, 1231
HincII	GTYRAC	2 cut(s)	744, 1087
HindII	GTYRAC	2 cut(s)	744, 1087
HinfI	GANTC	7 cut(s)	6, 47, 73, 572, 745, 937, 1262
HpaII	CCGG	4 cut(s)	204, 410, 513, 983
HphI	GGTGA	2 cut(s)	573, 638
Hpy166II	GTNNAC	3 cut(s)	744, 816, 1087
Hpy188I	TCNGA	5 cut(s)	46, 60, 613, 676, 781
Hpy188III	TCNNGA	7 cut(s)	254, 544, 647, 767, 990, 1053, 1310
Hpy8I	GTNNAC	3 cut(s)	744, 816, 1087
HpyAV	CCTTC	3 cut(s)	1038, 1050, 1121
HpyCH4III	ACNGT	5 cut(s)	91, 105, 479, 680, 764
HpyCH4IV	ACGT	2 cut(s)	554, 1109
HpyF10VI	GCNNNNNNNGC	4 cut(s)	231, 378, 497, 1295
HpyF3I	CTNAG	3 cut(s)	348, 738, 1003
HpySE526I	ACGT	2 cut(s)	554, 1109
Hsp92I	GRCGYC	1 cut(s)	1231
Hsp92II	CATG	7 cut(s)	198, 323, 432, 770, 877, 1104, 1289
HspAI	GCGC	3 cut(s)	808, 953, 1231
KasI	GGCGCC	1 cut(s)	1230
KpnI	GGTACC	1 cut(s)	1140
Ksp22I	TGATCA	1 cut(s)	1273
Kzo9I	GATC	3 cut(s)	640, 1063, 1273
LmnI	GCTCC	5 cut(s)	171, 269, 445, 1055, 1109
Lsp1109I	GCAGC	2 cut(s)	178, 1195
LweI	GCATC	3 cut(s)	415, 890, 1240
MabI	ACCWGGT	1 cut(s)	358
MaeI	CTAG	6 cut(s)	143, 918, 990, 1122, 1223, 1235
MaeII	ACGT	2 cut(s)	554, 1109
MaeIII	GTNAC	3 cut(s)	154, 479, 719
MalI	GATC	3 cut(s)	642, 1065, 1275
MboI	GATC	3 cut(s)	640, 1063, 1273
MboII	GAAGA	8 cut(s)	131, 140, 193, 299, 571, 668, 671, 1009
MlsI	TGGCCA	1 cut(s)	1284
MluCI	AATT	8 cut(s)	414, 531, 538, 547, 566, 852, 1201, 1244
MluNI	TGGCCA	1 cut(s)	1284
Mly113I	GGCGCC	1 cut(s)	1231
MlyI	GAGTC	2 cut(s)	739, 931
MmeI	TCCRAC	2 cut(s)	119, 158
Mox20I	TGGCCA	1 cut(s)	1284
Mph1103I	ATGCAT	2 cut(s)	430, 1291
MscI	TGGCCA	1 cut(s)	1284
MseI	TTAA	5 cut(s)	248, 417, 534, 839, 1215
Msp20I	TGGCCA	1 cut(s)	1284
MspA1I	CMGCKG	2 cut(s)	125, 1208
MspI	CCGG	4 cut(s)	204, 410, 513, 983
MspR9I	CCNGG	8 cut(s)	204, 205, 274, 360, 410, 513, 514, 984
Mva1269I	GAATGC	2 cut(s)	906, 1289
MvaI	CCWGG	2 cut(s)	274, 360
MwoI	GCNNNNNNNGC	4 cut(s)	231, 378, 497, 1295
NarI	GGCGCC	1 cut(s)	1231
NciI	CCSGG	6 cut(s)	204, 205, 410, 513, 514, 984
NdeI	CATATG	1 cut(s)	1300
NdeII	GATC	3 cut(s)	640, 1063, 1273
NlaIII	CATG	7 cut(s)	198, 323, 432, 770, 877, 1104, 1289
NlaIV	GGNNCC	4 cut(s)	271, 325, 1138, 1232
NmeAIII	GCCGAG	1 cut(s)	407
NmuCI	GTSAC	2 cut(s)	154, 719
NsbI	TGCGCA	1 cut(s)	954
NsiI	ATGCAT	2 cut(s)	430, 1291
NspI	RCATGY	2 cut(s)	198, 1104
PaeI	GCATGC	1 cut(s)	1104
PagI	TCATGA	1 cut(s)	766
PctI	GAATGC	2 cut(s)	906, 1289
PfeI	GAWTC	5 cut(s)	6, 47, 73, 572, 1262
PflMI	CCANNNNNTGG	1 cut(s)	333
PkrI	GCNGC	3 cut(s)	193, 1197, 1210
PleI	GAGTC	2 cut(s)	739, 931
PluTI	GGCGCC	1 cut(s)	1234
PpsI	GAGTC	2 cut(s)	739, 931
PshBI	ATTAAT	1 cut(s)	417
Psp6I	CCWGG	2 cut(s)	272, 358
PspGI	CCWGG	2 cut(s)	272, 358
PspN4I	GGNNCC	4 cut(s)	271, 325, 1138, 1232
PspPI	GGNCC	1 cut(s)	519
PstNI	CAGNNNCTG	2 cut(s)	226, 1211
PvuII	CAGCTG	1 cut(s)	1208
RsaI	GTAC	5 cut(s)	211, 476, 892, 1120, 1138
RsaNI	GTAC	5 cut(s)	210, 475, 891, 1119, 1137
SaqAI	TTAA	5 cut(s)	248, 417, 534, 839, 1215
SatI	GCNGC	3 cut(s)	192, 1196, 1209
Sau3AI	GATC	3 cut(s)	640, 1063, 1273
Sau96I	GGNCC	1 cut(s)	519
ScaI	AGTACT	1 cut(s)	1120
SchI	GAGTC	2 cut(s)	739, 931
ScrFI	CCNGG	8 cut(s)	204, 205, 274, 360, 410, 513, 514, 984
SexAI	ACCWGGT	1 cut(s)	358
SfaNI	GCATC	3 cut(s)	415, 890, 1240
SfoI	GGCGCC	1 cut(s)	1232
SinI	GGWCC	1 cut(s)	519
SmaI	CCCGGG	2 cut(s)	205, 514
SmlI	CTYRAG	2 cut(s)	619, 647
SmoI	CTYRAG	2 cut(s)	619, 647
SpeI	ACTAGT	1 cut(s)	1121
SphI	GCATGC	1 cut(s)	1104
Sse9I	AATT	8 cut(s)	414, 531, 538, 547, 566, 852, 1201, 1244
SsiI	CCGC	2 cut(s)	125, 1196
SspDI	GGCGCC	1 cut(s)	1230
SspI	AATATT	1 cut(s)	38
SspMI	CTAG	6 cut(s)	143, 918, 990, 1122, 1223, 1235
StyD4I	CCNGG	8 cut(s)	202, 203, 272, 358, 408, 511, 512, 982
TaaI	ACNGT	5 cut(s)	91, 105, 479, 680, 764
TaiI	ACGT	2 cut(s)	557, 1112
TaqI	TCGA	7 cut(s)	71, 312, 364, 570, 899, 935, 1248
TaqII	GACCGA	1 cut(s)	536
TasI	AATT	8 cut(s)	414, 531, 538, 547, 566, 852, 1201, 1244
TatI	WGTACW	1 cut(s)	1118
TauI	GCSGC	1 cut(s)	1198
TfiI	GAWTC	5 cut(s)	6, 47, 73, 572, 1262
Tru1I	TTAA	5 cut(s)	248, 417, 534, 839, 1215
Tru9I	TTAA	5 cut(s)	248, 417, 534, 839, 1215
TscAI	CASTG	5 cut(s)	83, 94, 227, 685, 979
TseFI	GTSAC	2 cut(s)	154, 719
TseI	GCWGC	2 cut(s)	191, 1208
Tsp45I	GTSAC	2 cut(s)	154, 719
TspDTI	ATGAA	4 cut(s)	42, 564, 862, 1164
TspGWI	ACGGA	4 cut(s)	719, 725, 833, 1175
TspMI	CCCGGG	2 cut(s)	203, 512
TspRI	CASTG	5 cut(s)	83, 94, 227, 685, 979
Van91I	CCANNNNNTGG	1 cut(s)	333
VpaK11BI	GGWCC	1 cut(s)	519
VspI	ATTAAT	1 cut(s)	417
XapI	RAATTY	4 cut(s)	538, 566, 1201, 1244
XbaI	TCTAGA	1 cut(s)	989
XceI	RCATGY	2 cut(s)	198, 1104
XmaI	CCCGGG	2 cut(s)	203, 512
XmiI	GTMKAC	1 cut(s)	815
XspI	CTAG	6 cut(s)	143, 918, 990, 1122, 1223, 1235
ZrmI	AGTACT	1 cut(s)	1120
Zsp2I	ATGCAT	2 cut(s)	430, 1291

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.