Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0016069

Physical Location & Seq

Genomic Coordinates Reverse (-)

60430692 .. 60438187

Gene Structure 7496 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0435600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4851 bp

ATGGCCTTGTTGGAATCCCCTACGGCCTCTGCATCACTTCCTGCTACATCAGTTGAATCATCGGCTCCTGGGTGGAAGTATGAGGTGTTTTTGAGTTTCATGGGTAAAGACACCTGGCGGGGTTTTACCTCTTATTTATACAATGAATTGCAAAACAGAGAAATCAAGACATTCATGGATATCGACCCAACTAGTTTGGATAGAGGAGAGGGGATTTCTCAAAGTACCCTGGAGGCCATTCAAGAATCAAGATTTGCAATCGTTGTTCTCTCGCAAAATTATGCTTCTTCCAGTTGGTGTTTGGATGAACTCACACAGATTATGCAGTGCATGGAAAATAATAAGGAAACAATTCTGCCAGTTTTCTATCATGTGGATCCCTCTCAAGTGCGCTCTCAAAGGGGGGTGTGCGGAGCGGCCTTGGCTGCACATGAAGAGCGGTTTAAAGATCAAAAACAGAGGCTGATGAAATGGAGAGACGCCTTGAACAAAATCAGCAGCATCTTTGGCTGGGTATGTGGGAGTTCTGAGTCAGAAGGACACCTTGTCCAACAAATCGCGGAGTTTGTCTGCAGCAAATCTATTGAGTCGACGGAAGATATTGAAACAAAACAAGCAATGGATGGAACTTCTCCTCGTTGGAAGTATGATGTGTTTATAAATTTCAGGGGTGAGGACACTTGTAAGGGTTTTCTGTCCTATTTACTAGCTGAATTGCAAAAGAAACCAGCACACCTGAACATGGTAAAGAATGATGAACCACTTCCAAAAGGGCGGAATATATCTTCATATCTCCTAAACGCCATAGAAGAGGTGAGGTTTGCCATTGTTGTTCTTTCGCAAGACTTTGCTTATTCATCATGGTGCCTAGAGGAACTCGCAAAGATTTTTGAATGCATGGGAAACCAAAATAGAATTCTGCCAATCTTTTATCATGTGGATCCCTCTGAGGTGCGACATCAAAGGGGGAGTTTTGGAGATGCCTTCGCTGTACATGAAGAAAGATTTCGGGATAATCATGAGAAGGTCAGGCGGTGGAGACATGTTTTGTCAATGGTGGCCAATCTCTCTGGATGGAATTCAGAGGATTATGAGTGTGAAGGAGAACTTATCAAAGCTGTTGGGGAAGCTATGTGCAGTAGACTACTTCAGTCTACAGAGTCAACAGAAGATTTTGAAGCAACAGGAGATGTCATGGACCGAACTTCTCCCCAGCCTGACCCAGCTCCTGTGTGGAAGAATGATGTGCTTTCGGGTTTCAGAATGGAAGACACTCGCAGGGGTTTTGTTGCCCATTCAGACCATGAATTGCCAAACACAGGACTAAGTAAAACATTCAAGGATGAAGACCAGCTTGAAAAAGGGGTAGCCCTCCCCTCTGCATCTCTTTCCTCAACTGAACCAAGTCCTCAACCAGCTCTTGGGCAGAAGAATGATGAGTTTTTGGGTTTCAGGGGAAACGACAATCAAAGGGGTTTTGTCACCCATTCAAGTCATGAAATACAAAACACCAGAGTGACTAAGACATTCACCGATGAAGAACATCTTGAAAAAGGGATAGCTGCGTCTCAGCCTTCATCAGCTGAACGAGATCCTCGGCCGAAGTATGATGTGTTTTTGAGCTTTAGGGGTGAAGACACTCGTAAGTGTTTTATAGCCTACTTATATCGTGAATTGCAAAACACCAGAGTAATTAGAACTTTCAAGGATGACGTACAACTACAAAAAGGGACAGTTATTTCTTCAAATCTCCTAACGGCAATTGAAGAATCAAGGTTTGCGATTATTGTGTTGTCGGAGAACTATGCTTCTTCTGCATGGTGTTTGGATGAACTTACAAAGATTTTTCAGTGCATGGAGGGTAAGGACACAATTCTCCCAATTTTTTATAAGTTGGATCCCGCTGTTGTACGATATCAAAACGAAGAGACAAGTTTTGGAGAAGCTTTCATTAAGCATGAAGCTAGACAAGACATTGAGAAGATGAACCAGTGGAGAGCTGCTTTAAAAGGAGTGGCTGGAATCTCTGGGTGGCATTCAACCAACTATCAGACGGATATAGAACTAATTGAAGACATTGTGAAACATTTGTGGGAGAAAGTACTTGAAGCACCCACCGGAAATTTTGAAACATTTGAAGCGACAAGGCAAGCCATGGATGAGGTTATGAAGGTATTAAAAGATGATCAAGTCACTGTCCTTGGGGTGCATGGAGTGGGTGGCGTTGGAAAGACAACGATGGTCAAACATGTTAGTGCAGAAGCCCAGAAAGGTGGGCTTTTTGATCATGTGATCATGGTTGTCGTATCCAAAAACCCTGACTTGGAAGAAATTCAAGGCACATTGGCAGATTCGCTGGGCTTGAAGGAGGGGATCATGAGAGGGAATAGGATGCTTATAATCTTGGATGACATTTGGGAGAGTCTTGAACTCTCGAGCATTGGAATTCCCAGTCACCATGAGCTTCAAAGGTGCAATTCTAAAGTTGTACTCACCACAAGGAAAAGCAACGTCTGCCATTCAATGGGGTGCCATGCCCTGATCCCTCTCAATGTACTGTCAGTGGTAGATTCTCATAAGTTATTAATGGATACGTTGGGGAAGCCCTTTGATGAATCTACCGTTTCCGATGGTGAAGAGTGGAAGGTAGCTCAAGAATGTGGTGGTCTTCCAAAAGCAATAAAAGCAGCTGCAAGGTCAATTGGAGATAAATACTTGGACGAATGGAATGAAGCAGTTGCAAGGGCATTTGGAGATGACTGCTTGGCCGAATGGAATGAAACAGCTCAACCCGTCAACTTCGAAGCTGGGGAAGAGGCATTTCGTTCATTGGCAACTGATGATGCCAAGTCATGCTTCTTGCTATGCTGCCTATTCCCAGAAAATTATGATATCCCAATTGAAGTGTTGCTCAGGTATGGGATTGGAATAGGTTTGTTTCAATATGATACAATACAAGAAGCCAGAGATAGAGCACATTCTCTGGTCAAGTACCTCAAAGCTTCCAGATTGCTTTTGGACGGTACAAAGAAGGGATGCATAAGGATGACTGATGACATTCGAAATCTGGCGTTGTCCAAAGAAGGTGATGGGTTTTTAGTAAAAGCTGGTTGTGAACTAGAGGAGTGGCCAGAGGCAGAGACAGAGGCAGGATGGCTGCAAGAATTCACTTCCTGGCCCTCGATTTGGACAAGAGATGCCCATGAAGGCTACTCAACAATCTCACTAATGAGAAACAAGCTTCACAAGCTTCCCGAATATTTGGTATGTTCAAAACTCCAAATTTTATTACTACAAAGTAATGCCGGAATATGTGACATCCCAGAAACCTTTTTTCAAAATCCTAATGCGCTAAGGGTCTTTGATCTTAGCTTCACTAGTGTTTCGTCACTACCTTCATCATTCAGTTTCCTAACCAACCTCCAAACTTTGAATTTGGATTTTTGCAAAAATTTAAGTAACATATCTGTTCTAGGAAAACTTAAGAAACTTGAGATTCTTAGTATGAGAGAACTTCCTCTTAAGAAATTTCCAAAACAAATAGGAGATTTGACCCGTCTAAGGATGTTGGACGTCACTGGTAGAGATCTTGAAACTATCCCATCTAAAGTGATATCGAGGTTGTATAGACTGCAAGAACTGCACATGAAATTTCAGTTTGGGACGTGGGGAAGTAAAGTTGAGGACGCATTTCATGAAGAAACCAATGCTGACTTTGATGAGTTGACTGGCTTGTTGGATTTAAACATTTTGGAAGTTTACATATCTGATGCAGCATGCTTGCCTACAAGTGTTGAGTACAAGCCGAATTGGGTAGAATTTGATATATGCATCGGCGGCCACGTCCCTCACGTGTTGCCCCCTCAACATGATGTTTTTTCAAGAACCTTGACTCTTAACACAAAGATCAATAGGTTACCGGATTGGTTTATCAGCGTTGTGACAGAGAGAGTGGAGAAGCTAGAGTACACAGATTGCGAAGACTTGAATGACGTCGTTGGGGAATACGACCATGGCAGATTACTTGCACTGAAATATCTCTCTGTGAACGGGAACCGTAAGAACTTGAAAGAGATAATCAACACAATGACACGGGTTTCAAATAAACCAATATTTGAGAACTTGGAAGAGTTGCATCTGTGCTGCTTATATTCCCTCAAGGAGTTATGTGTTGGTGAGCTACCACCTGGGTCTCTACGCAAGCTCAAGTTCTTGAAAGTGCAAACTTGTCTTAGCTTGGTGAATGCACTATTACCATCAACATTGTTGCAGAGACTACAAAATCTGGAAGAAATCATTTGTATAAATCTGAAAGGAATGAAATTTGTATTTGGATCTGATGAAGGGCTTGATCCAGAAAAGATTTCGAGGAAATTGAGGGAGATGAGATTTTCTGTTCTACCCGAACTAGTAAGCATATGTAATGGTCCTGCTCCGTCTGCAATCTTCCGGAATCTTAAAGTTTTGGCAATTTGCGAGTGCAAGGAGTTGAAAAATCTCTTCACAATAGATGTGGCTGAATGTCTTTTTCAGTTGGAAGACCTTTCCATAGAGCAGTGTCCTAGCTTGGACAGAGTCATTGAAGCAAGCAATAGCTTGAACAGCAAGATTAAACTTCCAGCGTTGAAGAAGTTAGCTTTGATAGATCTTCCGGCGCTCACAAGGTTCTGCACTGAAAGTGGTACTATTGAAACTGAGTGCCCTTCATTGGAATACTTGTACGTGGAGAAGTGCCTCCAGTTTTTGTATTTTGCTTACCACTTCGACAACAAGAACCAAGTCCACTTCAATGATGAACAACATTTGAGTTCTTTAGTGAAAAGGTGGGAGGAAGTGCATAATCGGCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0007275 GO:0008150 GO:0009555 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009908 GO:0009987 GO:0010026 GO:0010090 GO:0010468 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032989 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048468 GO:0048608 GO:0048653 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0071840 GO:0080090 GO:0090558 GO:0090567 GO:0090626 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-binding

Protein Analysis

1616

Amino Acids

183.07

Weight (kDa)

5.23

Isoelectric Point (pI)

42.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	26 - 204	3.4e-47	TIR domain
TIR_2	PF13676	29 - 128	1.2e-14	TIR domain
TIR	PF01582	215 - 393	1.6e-43	TIR domain
TIR_2	PF13676	218 - 315	4.4e-08	TIR domain
TIR	PF01582	535 - 706	2.6e-47	TIR domain
TIR_2	PF13676	538 - 634	1.9e-12	TIR domain
NB-ARC	PF00931	717 - 863	8.1e-23	NB-ARC domain
LRR_8	PF13855	1092 - 1149	8e-08	Leucine rich repeat
LRR_14	PF23598	1096 - 1215	5.8e-10	Leucine-rich repeat region
LRR_RPS2	PF23247	1375 - 1520	5.8e-23	Plant disease resistance protein RPS2-like, leucine-rich repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0016069)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G56110
fragaria_vesca	FvH4_3g41840
malus_domestica	MD03G1040500.v1.1 MD11G1041900.v1.1
prunus_persica	Prupe.6G032800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0075031
rosa_laevigata	RLG00000036519
rosa_multiflora	Rmu_sc0006984.1_g000003
rosa_roxburghii	Rroxscaffold_1G00006620
rosa_rugosa	Rorug05G0435600
rosa_samantha	Rh5AG492900 Rh5BG514200 Rh5CG537800 Rh5DG526500
rosa_wichuraiana	Rw5G045690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	659, 1890, 2399
AatII	GACGTC	2 cut(s)	3576, 3996
AccB1I	GGYRCC	2 cut(s)	864, 2529
AccB7I	CCANNNNNTGG	2 cut(s)	1421, 2524
AccBSI	CCGCTC	2 cut(s)	416, 439
AccI	GTMKAC	3 cut(s)	590, 1141, 1154
AccII	CGCG	1 cut(s)	560
AccIII	TCCGGA	1 cut(s)	4449
AciI	CCGC	9 cut(s)	118, 411, 416, 439, 560, 775, 1033, 1902, 3837
AcoI	YGGCCR	5 cut(s)	1059, 1598, 2766, 3128, 3838
AcuI	CTGAAG	1 cut(s)	1133
AcvI	CACGTG	1 cut(s)	3853
AcyI	GRCGYC	3 cut(s)	480, 3573, 3993
AfiI	CCNNNNNNNGG	8 cut(s)	742, 1319, 1421, 2323, 2524, 3186, 3561, 4708
AflII	CTTAAG	2 cut(s)	3482, 3521
AflIII	ACRYGT	3 cut(s)	1042, 2248, 3852
AhdI	GACNNNNNGTC	1 cut(s)	545
AhlI	ACTAGT	3 cut(s)	191, 3377, 4408
AjiI	CACGTC	2 cut(s)	3666, 3844
AjnI	CCWGG	5 cut(s)	67, 113, 228, 3173, 4186
AjuI	GAANNNNNNNTTGG	4 cut(s)	900, 932, 2840, 2872
AleI	CACNNNNGTG	1 cut(s)	1514
Alw21I	GWGCWC	1 cut(s)	2977
Alw26I	GTCTC	7 cut(s)	471, 1033, 1572, 1922, 3134, 4197, 4267
AlwNI	CAGNNNCTG	2 cut(s)	463, 1229
Ama87I	CYCGRG	1 cut(s)	2434
Aor13HI	TCCGGA	1 cut(s)	4449
AseI	ATTAAT	1 cut(s)	2585
Asp700I	GAANNNNTTC	4 cut(s)	351, 762, 1005, 2331
AspLEI	GCGC	3 cut(s)	393, 3352, 4657
AspS9I	GGNCC	3 cut(s)	1198, 3177, 4427
AsuII	TTCGAA	2 cut(s)	2802, 3061
AvaI	CYCGRG	1 cut(s)	2434
AvaII	GGWCC	2 cut(s)	1198, 4427
BaeGI	GKGCMC	1 cut(s)	4703
BalI	TGGCCA	2 cut(s)	1061, 3130
BamHI	GGATCC	3 cut(s)	376, 940, 1897
BanI	GGYRCC	2 cut(s)	864, 2529
BarI	GAAGNNNNNNTAC	2 cut(s)	3950, 3982
BbrPI	CACGTG	1 cut(s)	3853
BbsI	GAAGAC	7 cut(s)	1275, 1353, 1641, 2079, 2660, 3987, 4545
Bbv12I	GWGCWC	1 cut(s)	2977
BccI	CCATC	8 cut(s)	617, 1068, 2233, 2624, 3083, 3147, 3610, 4264
BceAI	ACGGC	2 cut(s)	39, 1773
BciT130I	CCWGG	5 cut(s)	69, 115, 230, 3175, 4188
BciVI	GTATCC	2 cut(s)	2317, 2584
BclI	TGATCA	3 cut(s)	2185, 2284, 2292
BcoDI	GTCTC	7 cut(s)	471, 1033, 1572, 1922, 3134, 4197, 4267
BcuI	ACTAGT	3 cut(s)	191, 3377, 4408
BfmI	CTRYAG	2 cut(s)	571, 1155
BfoI	RGCGCY	1 cut(s)	4658
BfrI	CTTAAG	2 cut(s)	3482, 3521
BfuI	GTATCC	2 cut(s)	2317, 2584
BglII	AGATCT	2 cut(s)	3586, 4645
BmcAI	AGTACT	1 cut(s)	2103
Bme1390I	CCNGG	5 cut(s)	69, 115, 230, 3175, 4188
Bme18I	GGWCC	2 cut(s)	1198, 4427
BmeRI	GACNNNNNGTC	1 cut(s)	545
BmeT110I	CYCGRG	1 cut(s)	2434
BmgBI	CACGTC	2 cut(s)	3666, 3844
BmgT120I	GGNCC	3 cut(s)	1198, 3177, 4427
BmiI	GGNNCC	7 cut(s)	66, 378, 866, 942, 1899, 2531, 4055
BmrFI	CCNGG	5 cut(s)	69, 115, 230, 3175, 4188
BmrI	ACTGGG	1 cut(s)	2445
BmuI	ACTGGG	1 cut(s)	2445
BpiI	GAAGAC	7 cut(s)	1275, 1353, 1641, 2079, 2660, 3987, 4545
BpmI	CTGGAG	2 cut(s)	251, 4721
Bpu10I	CCTNAGC	2 cut(s)	2912, 3353
Bpu14I	TTCGAA	2 cut(s)	2802, 3061
BpuEI	CTTGAG	5 cut(s)	369, 2637, 3512, 4142, 4190
BsaAI	YACGTR	2 cut(s)	3853, 4723
BsaHI	GRCGYC	3 cut(s)	480, 3573, 3993
BsaI	GGTCTC	1 cut(s)	4197
BsaJI	CCNNGG	8 cut(s)	68, 228, 420, 1595, 2154, 2200, 4012, 4187
BsaWI	WCCGGW	3 cut(s)	2117, 3919, 4449
Bsc4I	CCNNNNNNNGG	8 cut(s)	742, 1319, 1421, 2323, 2524, 3186, 3561, 4708
Bse1I	ACTGG	7 cut(s)	291, 359, 1990, 2451, 3583, 3733, 4738
Bse3DI	GCAATG	1 cut(s)	624
BseAI	TCCGGA	1 cut(s)	4449
BseBI	CCWGG	5 cut(s)	69, 115, 230, 3175, 4188
BseDI	CCNNGG	8 cut(s)	68, 228, 420, 1595, 2154, 2200, 4012, 4187
BseLI	CCNNNNNNNGG	8 cut(s)	742, 1319, 1421, 2323, 2524, 3186, 3561, 4708
BseMI	GCAATG	1 cut(s)	624
BseMII	CTCAG	5 cut(s)	519, 939, 1583, 2926, 4686
BseNI	ACTGG	7 cut(s)	291, 359, 1990, 2451, 3583, 3733, 4738
BseRI	GAGGAG	3 cut(s)	219, 624, 3137
BseSI	GKGCMC	1 cut(s)	4703
BseX3I	CGGCCG	1 cut(s)	1598
BseYI	CCCAGC	5 cut(s)	510, 1212, 1222, 2356, 2807
BsgI	GTGCAG	5 cut(s)	411, 1156, 2277, 3626, 4654
Bsh1236I	CGCG	1 cut(s)	560
Bsh1285I	CGRYCG	1 cut(s)	1601
BshNI	GGYRCC	2 cut(s)	864, 2529
BsiEI	CGRYCG	1 cut(s)	1601
BsiHKAI	GWGCWC	1 cut(s)	2977
BsiHKCI	CYCGRG	1 cut(s)	2434
BsiSI	CCGG	5 cut(s)	2118, 3306, 3920, 4450, 4652
BslFI	GGGAC	3 cut(s)	1744, 3676, 3830
BslI	CCNNNNNNNGG	8 cut(s)	742, 1319, 1421, 2323, 2524, 3186, 3561, 4708
BsmAI	GTCTC	7 cut(s)	471, 1033, 1572, 1922, 3134, 4197, 4267
BsmBI	CGTCTC	2 cut(s)	471, 1572
BsmFI	GGGAC	3 cut(s)	1744, 3676, 3830
BsmI	GAATGC	3 cut(s)	899, 2035, 4249
Bso31I	GGTCTC	1 cut(s)	4197
BsoBI	CYCGRG	1 cut(s)	2434
Bsp119I	TTCGAA	2 cut(s)	2802, 3061
Bsp1286I	GDGCHC	2 cut(s)	2977, 4703
Bsp13I	TCCGGA	1 cut(s)	4449
Bsp1407I	TGTACA	1 cut(s)	991
Bsp19I	CCATGG	2 cut(s)	2154, 4012
BspACI	CCGC	9 cut(s)	118, 411, 416, 439, 560, 775, 1033, 1902, 3837
BspCNI	CTCAG	5 cut(s)	520, 940, 1582, 2925, 4687
BspEI	TCCGGA	1 cut(s)	4449
BspFNI	CGCG	1 cut(s)	560
BspHI	TCATGA	4 cut(s)	1018, 1495, 2376, 3694
BspLI	GGNNCC	7 cut(s)	66, 378, 866, 942, 1899, 2531, 4055
BspMAI	CTGCAG	1 cut(s)	575
BspQI	GCTCTTC	1 cut(s)	429
BspT104I	TTCGAA	2 cut(s)	2802, 3061
BspT107I	GGYRCC	2 cut(s)	864, 2529
BspTI	CTTAAG	2 cut(s)	3482, 3521
BspTNI	GGTCTC	1 cut(s)	4197
BsrBI	CCGCTC	2 cut(s)	416, 439
BsrDI	GCAATG	1 cut(s)	624
BsrGI	TGTACA	1 cut(s)	991
BsrI	ACTGG	7 cut(s)	291, 359, 1990, 2451, 3583, 3733, 4738
BssECI	CCNNGG	8 cut(s)	68, 228, 420, 1595, 2154, 2200, 4012, 4187
BssNI	GRCGYC	3 cut(s)	480, 3573, 3993
BssT1I	CCWWGG	4 cut(s)	420, 2154, 2200, 4012
Bst2UI	CCWGG	5 cut(s)	69, 115, 230, 3175, 4188
Bst4CI	ACNGT	6 cut(s)	1735, 2197, 2559, 2623, 3023, 4058
Bst6I	CTCTTC	7 cut(s)	429, 804, 1920, 2631, 2808, 4122, 4505
BstACI	GRCGYC	3 cut(s)	480, 3573, 3993
BstAFI	CTTAAG	2 cut(s)	3482, 3521
BstAPI	GCANNNNNTGC	2 cut(s)	2514, 3640
BstAUI	TGTACA	1 cut(s)	991
BstBAI	YACGTR	2 cut(s)	3853, 4723
BstBI	TTCGAA	2 cut(s)	2802, 3061
BstC8I	GCNNGC	5 cut(s)	2151, 3778, 3782, 4202, 4588
BstDSI	CCRYGG	2 cut(s)	2154, 4012
BstEII	GGTNACC	1 cut(s)	3915
BstFNI	CGCG	1 cut(s)	560
BstH2I	RGCGCY	1 cut(s)	4658
BstHHI	GCGC	3 cut(s)	393, 3352, 4657
BstMAI	GTCTC	7 cut(s)	471, 1033, 1572, 1922, 3134, 4197, 4267
BstMCI	CGRYCG	1 cut(s)	1601
BstNI	CCWGG	5 cut(s)	69, 115, 230, 3175, 4188
BstNSI	RCATGY	3 cut(s)	1046, 2252, 3780
BstPI	GGTNACC	1 cut(s)	3915
BstSCI	CCNGG	5 cut(s)	67, 113, 228, 3173, 4186
BstSFI	CTRYAG	2 cut(s)	571, 1155
BstSLI	GKGCMC	1 cut(s)	4703
BstUI	CGCG	1 cut(s)	560
BstV2I	GAAGAC	7 cut(s)	1275, 1353, 1641, 2079, 2660, 3987, 4545
BstX2I	RGATCY	7 cut(s)	376, 940, 1591, 1897, 3586, 4334, 4645
BstXI	CCANNNNNNTGG	1 cut(s)	2273
BstYI	RGATCY	7 cut(s)	376, 940, 1591, 1897, 3586, 4334, 4645
BstZI	CGGCCG	1 cut(s)	1598
BsuI	GTATCC	2 cut(s)	2317, 2584
BtgI	CCRYGG	2 cut(s)	2154, 4012
BtrI	CACGTC	2 cut(s)	3666, 3844
BtsI	GCAGTG	2 cut(s)	332, 4562
BtsIMutI	CAGTG	9 cut(s)	332, 1856, 1997, 2193, 2568, 3576, 4028, 4562, 4671
Cac8I	GCNNGC	5 cut(s)	2151, 3778, 3782, 4202, 4588
CaiI	CAGNNNCTG	2 cut(s)	463, 1229
CciI	TCATGA	4 cut(s)	1018, 1495, 2376, 3694
CfoI	GCGC	3 cut(s)	393, 3352, 4657
Cfr13I	GGNCC	3 cut(s)	1198, 3177, 4427
CseI	GACGC	3 cut(s)	488, 1554, 3695
CspCI	CAANNNNNGTGG	2 cut(s)	4494, 4529
DraI	TTTAAA	3 cut(s)	445, 2007, 3744
DriI	GACNNNNNGTC	1 cut(s)	545
EaeI	YGGCCR	5 cut(s)	1059, 1598, 2766, 3128, 3838
EagI	CGGCCG	1 cut(s)	1598
Eam1104I	CTCTTC	7 cut(s)	429, 804, 1920, 2631, 2808, 4122, 4505
Eam1105I	GACNNNNNGTC	1 cut(s)	545
EarI	CTCTTC	7 cut(s)	429, 804, 1920, 2631, 2808, 4122, 4505
EciI	GGCGGA	1 cut(s)	790
EclXI	CGGCCG	1 cut(s)	1598
Eco130I	CCWWGG	4 cut(s)	420, 2154, 2200, 4012
Eco31I	GGTCTC	1 cut(s)	4197
Eco32I	GATATC	4 cut(s)	181, 1916, 2893, 3615
Eco47I	GGWCC	2 cut(s)	1198, 4427
Eco52I	CGGCCG	1 cut(s)	1598
Eco57I	CTGAAG	1 cut(s)	1133
Eco72I	CACGTG	1 cut(s)	3853
Eco88I	CYCGRG	1 cut(s)	2434
Eco91I	GGTNACC	1 cut(s)	3915
EcoO65I	GGTNACC	1 cut(s)	3915
EcoRI	GAATTC	4 cut(s)	915, 1078, 2445, 3164
EcoRII	CCWGG	5 cut(s)	67, 113, 228, 3173, 4186
EcoRV	GATATC	4 cut(s)	181, 1916, 2893, 3615
EcoT14I	CCWWGG	4 cut(s)	420, 2154, 2200, 4012
EcoT22I	ATGCAT	3 cut(s)	899, 3041, 3833
ErhI	CCWWGG	4 cut(s)	420, 2154, 2200, 4012
Esp3I	CGTCTC	2 cut(s)	471, 1572
FaqI	GGGAC	3 cut(s)	1744, 3676, 3830
FauI	CCCGC	2 cut(s)	111, 1909
FauNDI	CATATG	1 cut(s)	4418
FbaI	TGATCA	3 cut(s)	2185, 2284, 2292
FblI	GTMKAC	3 cut(s)	590, 1141, 1154
GlaI	GCGC	3 cut(s)	392, 3351, 4656
GsaI	CCCAGC	5 cut(s)	514, 1216, 1226, 2360, 2811
GsuI	CTGGAG	2 cut(s)	251, 4721
HaeII	RGCGCY	1 cut(s)	4658
HapII	CCGG	5 cut(s)	2118, 3306, 3920, 4450, 4652
HgaI	GACGC	3 cut(s)	488, 1554, 3695
HhaI	GCGC	3 cut(s)	393, 3352, 4657
Hin1I	GRCGYC	3 cut(s)	480, 3573, 3993
Hin6I	GCGC	3 cut(s)	391, 3350, 4655
HinP1I	GCGC	3 cut(s)	391, 3350, 4655
HincII	GTYRAC	4 cut(s)	591, 1164, 2797, 3726
HindII	GTYRAC	4 cut(s)	591, 1164, 2797, 3726
HindIII	AAGCTT	4 cut(s)	1944, 3000, 3239, 3248
HpaII	CCGG	5 cut(s)	2118, 3306, 3920, 4450, 4652
Hpy99I	CGWCG	2 cut(s)	595, 3998
HpyCH4III	ACNGT	6 cut(s)	1735, 2197, 2559, 2623, 3023, 4058
HpyCH4IV	ACGT	9 cut(s)	1713, 2511, 2594, 3573, 3665, 3843, 3852, 3993, 4722
HpySE526I	ACGT	9 cut(s)	1713, 2511, 2594, 3573, 3665, 3843, 3852, 3993, 4722
Hsp92I	GRCGYC	3 cut(s)	480, 3573, 3993
HspAI	GCGC	3 cut(s)	391, 3350, 4655
Kpn2I	TCCGGA	1 cut(s)	4449
Ksp22I	TGATCA	3 cut(s)	2185, 2284, 2292
LguI	GCTCTTC	1 cut(s)	429
LmnI	GCTCC	4 cut(s)	70, 413, 1231, 4438
MaeII	ACGT	9 cut(s)	1713, 2511, 2594, 3573, 3665, 3843, 3852, 3993, 4722
MbiI	CCGCTC	2 cut(s)	416, 439
MfeI	CAATTG	3 cut(s)	1761, 2700, 2898
MflI	RGATCY	7 cut(s)	376, 940, 1591, 1897, 3586, 4334, 4645
MhlI	GDGCHC	2 cut(s)	2977, 4703
MlsI	TGGCCA	2 cut(s)	1061, 3130
MluNI	TGGCCA	2 cut(s)	1061, 3130
MlyI	GAGTC	6 cut(s)	539, 596, 1169, 2431, 3886, 4584
MmeI	TCCRAC	8 cut(s)	574, 620, 1776, 1875, 2206, 3549, 3717, 4515
Mox20I	TGGCCA	2 cut(s)	1061, 3130
Mph1103I	ATGCAT	3 cut(s)	899, 3041, 3833
MroI	TCCGGA	1 cut(s)	4449
MroXI	GAANNNNTTC	4 cut(s)	351, 762, 1005, 2331
MscI	TGGCCA	2 cut(s)	1061, 3130
MslI	CAYNNNNRTG	5 cut(s)	862, 1514, 2253, 3044, 4788
Msp20I	TGGCCA	2 cut(s)	1061, 3130
MspA1I	CMGCKG	3 cut(s)	1583, 1904, 2690
MspCI	CTTAAG	2 cut(s)	3482, 3521
MspI	CCGG	5 cut(s)	2118, 3306, 3920, 4450, 4652
MspR9I	CCNGG	5 cut(s)	69, 115, 230, 3175, 4188
MunI	CAATTG	3 cut(s)	1761, 2700, 2898
Mva1269I	GAATGC	3 cut(s)	899, 2035, 4249
MvaI	CCWGG	5 cut(s)	69, 115, 230, 3175, 4188
MvnI	CGCG	1 cut(s)	560
NcoI	CCATGG	2 cut(s)	2154, 4012
NdeI	CATATG	1 cut(s)	4418
NlaIV	GGNNCC	7 cut(s)	66, 378, 866, 942, 1899, 2531, 4055
NmeAIII	GCCGAG	1 cut(s)	1576
NmuCI	GTSAC	8 cut(s)	1480, 1516, 2191, 2453, 3314, 3387, 3574, 3940
NsiI	ATGCAT	3 cut(s)	899, 3041, 3833
NspI	RCATGY	3 cut(s)	1046, 2252, 3780
NspV	TTCGAA	2 cut(s)	2802, 3061
OliI	CACNNNNGTG	1 cut(s)	1514
PaeI	GCATGC	1 cut(s)	3780
PaeR7I	CTCGAG	1 cut(s)	2434
PagI	TCATGA	4 cut(s)	1018, 1495, 2376, 3694
PciI	ACATGT	2 cut(s)	1042, 2248
PciSI	GCTCTTC	1 cut(s)	429
PcsI	WCGNNNNNNNCGW	2 cut(s)	3840, 3849
PctI	GAATGC	3 cut(s)	899, 2035, 4249
PdmI	GAANNNNTTC	4 cut(s)	351, 762, 1005, 2331
PflFI	GACNNNGTC	1 cut(s)	4574
PflMI	CCANNNNNTGG	2 cut(s)	1421, 2524
PleI	GAGTC	6 cut(s)	538, 595, 1168, 2430, 3886, 4583
PmaCI	CACGTG	1 cut(s)	3853
PmlI	CACGTG	1 cut(s)	3853
PpsI	GAGTC	6 cut(s)	538, 595, 1168, 2430, 3886, 4583
Ppu21I	YACGTR	2 cut(s)	3853, 4723
PscI	ACATGT	2 cut(s)	1042, 2248
PshBI	ATTAAT	1 cut(s)	2585
PsiI	TTATAA	3 cut(s)	659, 1890, 2399
Psp6I	CCWGG	5 cut(s)	67, 113, 228, 3173, 4186
PspCI	CACGTG	1 cut(s)	3853
PspEI	GGTNACC	1 cut(s)	3915
PspFI	CCCAGC	5 cut(s)	510, 1212, 1222, 2356, 2807
PspGI	CCWGG	5 cut(s)	67, 113, 228, 3173, 4186
PspN4I	GGNNCC	7 cut(s)	66, 378, 866, 942, 1899, 2531, 4055
PspPI	GGNCC	3 cut(s)	1198, 3177, 4427
PstI	CTGCAG	1 cut(s)	575
PstNI	CAGNNNCTG	2 cut(s)	463, 1229
PsuI	RGATCY	7 cut(s)	376, 940, 1591, 1897, 3586, 4334, 4645
PsyI	GACNNNGTC	1 cut(s)	4574
PvuII	CAGCTG	2 cut(s)	1583, 2690
RseI	CAYNNNNRTG	5 cut(s)	862, 1514, 2253, 3044, 4788
SalI	GTCGAC	1 cut(s)	589
SapI	GCTCTTC	1 cut(s)	429
Sau96I	GGNCC	3 cut(s)	1198, 3177, 4427
ScaI	AGTACT	1 cut(s)	2103
SchI	GAGTC	6 cut(s)	539, 596, 1169, 2431, 3886, 4584
ScrFI	CCNGG	5 cut(s)	69, 115, 230, 3175, 4188
SduI	GDGCHC	2 cut(s)	2977, 4703
SfcI	CTRYAG	2 cut(s)	571, 1155
Sfr274I	CTCGAG	1 cut(s)	2434
SfuI	TTCGAA	2 cut(s)	2802, 3061
SinI	GGWCC	2 cut(s)	1198, 4427
SlaI	CTCGAG	1 cut(s)	2434
SmiMI	CAYNNNNRTG	5 cut(s)	862, 1514, 2253, 3044, 4788
SmlI	CTYRAG	8 cut(s)	384, 2434, 2652, 3482, 3491, 3521, 4157, 4205
SmoI	CTYRAG	8 cut(s)	384, 2434, 2652, 3482, 3491, 3521, 4157, 4205
SpeI	ACTAGT	3 cut(s)	191, 3377, 4408
SphI	GCATGC	1 cut(s)	3780
SsiI	CCGC	9 cut(s)	118, 411, 416, 439, 560, 775, 1033, 1902, 3837
SspI	AATATT	2 cut(s)	3260, 4113
StyD4I	CCNGG	5 cut(s)	67, 113, 228, 3173, 4186
StyI	CCWWGG	4 cut(s)	420, 2154, 2200, 4012
TaaI	ACNGT	6 cut(s)	1735, 2197, 2559, 2623, 3023, 4058
TaiI	ACGT	9 cut(s)	1716, 2514, 2597, 3576, 3668, 3846, 3855, 3996, 4725
TaqI	TCGA	9 cut(s)	183, 590, 2435, 2802, 3061, 3182, 3617, 4367, 4764
TaqII	GACCGA	1 cut(s)	1215
TatI	WGTACW	6 cut(s)	991, 2101, 2488, 2554, 3798, 3966
TauI	GCSGC	2 cut(s)	419, 3840
TscAI	CASTG	9 cut(s)	332, 1856, 1997, 2200, 2568, 3583, 4035, 4562, 4678
TseFI	GTSAC	8 cut(s)	1480, 1516, 2191, 2453, 3314, 3387, 3574, 3940
Tsp45I	GTSAC	8 cut(s)	1480, 1516, 2191, 2453, 3314, 3387, 3574, 3940
TspGWI	ACGGA	3 cut(s)	608, 2069, 4425
TspRI	CASTG	9 cut(s)	332, 1856, 1997, 2200, 2568, 3583, 4035, 4562, 4678
Tth111I	GACNNNGTC	1 cut(s)	4574
Van91I	CCANNNNNTGG	2 cut(s)	1421, 2524
Vha464I	CTTAAG	2 cut(s)	3482, 3521
VpaK11BI	GGWCC	2 cut(s)	1198, 4427
VspI	ATTAAT	1 cut(s)	2585
XceI	RCATGY	3 cut(s)	1046, 2252, 3780
XcmI	CCANNNNNNNNNTGG	3 cut(s)	298, 1230, 3013
XhoI	CTCGAG	1 cut(s)	2434
XmiI	GTMKAC	3 cut(s)	590, 1141, 1154
XmnI	GAANNNNTTC	4 cut(s)	351, 762, 1005, 2331
ZraI	GACGTC	2 cut(s)	3574, 3994
ZrmI	AGTACT	1 cut(s)	2103
Zsp2I	ATGCAT	3 cut(s)	899, 3041, 3833

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.