Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0016069

Physical Location & Seq

Genomic Coordinates Forward (+)

3207301 .. 3208557

Gene Structure 1257 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1040500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 990 bp

ATGGGTCGGATTCCATGCTGTGAGAAGGACAACGTGAAAAGGGGGCAGTGGACTCCTGAAGAAGATAACAAGCTCTCTTCCTACATTGCCCAACATGGCACTCGTAATTGGCGCCTCATCCCCAAGAACGCTGGTTTACAAAGATGTGGGAAGAGCTGCAGGCTACGTTGGACGAACTACCTTCGCCCGGACCTTAAGCACGGCCAATTCTCCGACGCGGAAGAGCAAACAATTGTCAAGTACCACTCCGTTGTTGGCAACCGATGGTCGTTGATTGCAGCGCAACTGCCTGGCCGCACCGACAACGACGTGAAAAATCACTGGAACACCAAGCTGAAGAAGAAGCTATCGGGCATGGGGATCGACCCCGTCACACACAAACCCTTTTCGCACCTCATGGCCGAGATTGCAACCACACTAGCACCGCCGCAGGTGGCTCACCTCGCAGAAGCAGCCCTCGGCTGCTTTAAGGACGAAATGCTCCACCTCCTCATGAAGAAGCGCATCGACTTCCACCAACATACCAATCCAACGCCGGGAAACAGCACTGCCACGTACATTAACAGCCAACAGGAAGAAAAGCTCGACACCGTCAAAAAGATCAAGATTGGTTTATCTAGGGCAATGCAAGAACAACCGGACATGTTAACCTCAAACAAACCTTGGATGGACACTATTGGAGCAACATCTGCGAACTTCACAGGGAACTGCAGTGATTTCCCCTTGCCCATGTCTGGATTTCAGTACGGCCATTCCACGTTTGGGAACGAAGGCGATGGATCGCCGTGGAGCCAGAGTCTGTGTACCGGAAGTACGTGCACGGGGGCGGCGGACCAGCAGGGCCAGTTGGCTGACAAACTGGAGGTGGAAGAAAACGGAGAGGAGTCCGAGGGTAGAAAGGAGCTGAGAAACGGATCATCCAACATATTCAACTCGGATAGTCTCTTGTGGGATTTACCATCTGAAGATCTAATGAATCAGATGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0007275 GO:0008150 GO:0009555 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009908 GO:0009987 GO:0010026 GO:0010090 GO:0010468 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032989 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048468 GO:0048608 GO:0048653 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0071840 GO:0080090 GO:0090558 GO:0090567 GO:0090626 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-binding

Protein Analysis

330

Amino Acids

36.75

Weight (kDa)

6.6

Isoelectric Point (pI)

37.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	14 - 61	2.3e-17	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	17 - 77	2.3e-13	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	68 - 110	1.5e-14	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	70 - 117	1.1e-08	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0016069)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G56110
fragaria_vesca	FvH4_3g41840
malus_domestica	MD03G1040500.v1.1 MD11G1041900.v1.1
prunus_persica	Prupe.6G032800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0075031
rosa_laevigata	RLG00000036519
rosa_multiflora	Rmu_sc0006984.1_g000003
rosa_roxburghii	Rroxscaffold_1G00006620
rosa_rugosa	Rorug05G0435600
rosa_samantha	Rh5AG492900 Rh5BG514200 Rh5CG537800 Rh5DG526500
rosa_wichuraiana	Rw5G045690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	421
Acc36I	ACCTGC	1 cut(s)	421
AccB1I	GGYRCC	1 cut(s)	111
AccII	CGCG	1 cut(s)	218
AciI	CCGC	6 cut(s)	218, 295, 425, 428, 827, 830
AclWI	GGATC	3 cut(s)	368, 787, 922
AcoI	YGGCCR	4 cut(s)	202, 292, 399, 748
AcuI	CTGAAG	3 cut(s)	78, 356, 984
AcyI	GRCGYC	1 cut(s)	112
AfaI	GTAC	5 cut(s)	242, 557, 746, 805, 814
AfiI	CCNNNNNNNGG	4 cut(s)	187, 536, 734, 762
AflII	CTTAAG	1 cut(s)	194
AflIII	ACRYGT	1 cut(s)	642
AgsI	TTSAA	1 cut(s)	931
AjiI	CACGTC	1 cut(s)	310
AjnI	CCWGG	1 cut(s)	289
AluBI	AGCT	6 cut(s)	73, 156, 334, 346, 583, 904
AluI	AGCT	6 cut(s)	73, 156, 334, 346, 583, 904
Alw21I	GWGCWC	1 cut(s)	821
Alw26I	GTCTC	1 cut(s)	947
Alw44I	GTGCAC	1 cut(s)	817
AlwI	GGATC	3 cut(s)	368, 787, 922
AlwNI	CAGNNNCTG	1 cut(s)	799
AoxI	GGCC	5 cut(s)	202, 292, 399, 748, 841
ApaLI	GTGCAC	1 cut(s)	817
ApeKI	GCWGC	4 cut(s)	156, 278, 452, 462
AspLEI	GCGC	3 cut(s)	114, 283, 504
AspS9I	GGNCC	3 cut(s)	190, 832, 841
AsuC2I	CCSGG	2 cut(s)	188, 537
AsuHPI	GGTGA	1 cut(s)	431
AvaII	GGWCC	2 cut(s)	190, 832
BaeGI	GKGCMC	1 cut(s)	821
BanI	GGYRCC	1 cut(s)	111
Bbv12I	GWGCWC	1 cut(s)	821
BbvI	GCAGC	4 cut(s)	143, 290, 449, 464
BccI	CCATC	5 cut(s)	258, 661, 770, 967, 976
BceAI	ACGGC	3 cut(s)	217, 763, 769
BcgI	CGANNNNNNTGC	2 cut(s)	343, 377
BciT130I	CCWGG	1 cut(s)	291
BcnI	CCSGG	2 cut(s)	188, 537
BcoDI	GTCTC	1 cut(s)	947
BfaI	CTAG	2 cut(s)	419, 618
BfmI	CTRYAG	2 cut(s)	157, 709
BfoI	RGCGCY	1 cut(s)	115
BfrI	CTTAAG	1 cut(s)	194
BfuAI	ACCTGC	1 cut(s)	421
BglII	AGATCT	1 cut(s)	967
BisI	GCNGC	7 cut(s)	157, 279, 295, 428, 453, 463, 828
BlsI	GCNGC	7 cut(s)	158, 280, 296, 429, 454, 464, 829
Bme1390I	CCNGG	3 cut(s)	188, 291, 537
Bme18I	GGWCC	2 cut(s)	190, 832
BmgBI	CACGTC	1 cut(s)	310
BmgT120I	GGNCC	3 cut(s)	190, 832, 841
BmiI	GGNNCC	2 cut(s)	113, 791
BmrFI	CCNGG	3 cut(s)	188, 291, 537
BmsI	GCATC	1 cut(s)	513
BpmI	CTGGAG	1 cut(s)	881
BpuMI	CCSGG	2 cut(s)	188, 537
BsaAI	YACGTR	2 cut(s)	555, 816
BsaHI	GRCGYC	1 cut(s)	112
BsaJI	CCNNGG	4 cut(s)	457, 662, 785, 888
BsaWI	WCCGGW	2 cut(s)	637, 806
BsaXI	ACNNNNNCTCC	2 cut(s)	781, 811
Bsc4I	CCNNNNNNNGG	4 cut(s)	187, 536, 734, 762
Bse1I	ACTGG	3 cut(s)	326, 844, 864
Bse3DI	GCAATG	2 cut(s)	84, 630
BseBI	CCWGG	1 cut(s)	291
BseDI	CCNNGG	4 cut(s)	457, 662, 785, 888
BseGI	GGATG	3 cut(s)	117, 672, 917
BseLI	CCNNNNNNNGG	4 cut(s)	187, 536, 734, 762
BseMI	GCAATG	2 cut(s)	84, 630
BseMII	CTCAG	1 cut(s)	896
BseNI	ACTGG	3 cut(s)	326, 844, 864
BseRI	GAGGAG	2 cut(s)	479, 896
BseSI	GKGCMC	1 cut(s)	821
BseXI	GCAGC	4 cut(s)	143, 290, 449, 464
Bsh1236I	CGCG	1 cut(s)	218
BshFI	GGCC	5 cut(s)	204, 294, 401, 750, 843
BshNI	GGYRCC	1 cut(s)	111
BsiHKAI	GWGCWC	1 cut(s)	821
BsiSI	CCGG	4 cut(s)	188, 536, 638, 807
BslI	CCNNNNNNNGG	4 cut(s)	187, 536, 734, 762
BsmAI	GTCTC	1 cut(s)	947
BsnI	GGCC	5 cut(s)	204, 294, 401, 750, 843
Bsp1286I	GDGCHC	1 cut(s)	821
Bsp143I	GATC	5 cut(s)	360, 600, 779, 914, 967
BspACI	CCGC	6 cut(s)	218, 295, 425, 428, 827, 830
BspANI	GGCC	5 cut(s)	204, 294, 401, 750, 843
BspCNI	CTCAG	1 cut(s)	897
BspFNI	CGCG	1 cut(s)	218
BspHI	TCATGA	1 cut(s)	492
BspLI	GGNNCC	2 cut(s)	113, 791
BspMAI	CTGCAG	2 cut(s)	161, 713
BspMI	ACCTGC	1 cut(s)	421
BspPI	GGATC	3 cut(s)	368, 787, 922
BspQI	GCTCTTC	2 cut(s)	146, 216
BspT107I	GGYRCC	1 cut(s)	111
BspTI	CTTAAG	1 cut(s)	194
BsrDI	GCAATG	2 cut(s)	84, 630
BsrI	ACTGG	3 cut(s)	326, 844, 864
BssECI	CCNNGG	4 cut(s)	457, 662, 785, 888
BssMI	GATC	5 cut(s)	360, 600, 779, 914, 967
BssNI	GRCGYC	1 cut(s)	112
BssT1I	CCWWGG	1 cut(s)	662
Bst2UI	CCWGG	1 cut(s)	291
Bst4CI	ACNGT	1 cut(s)	592
Bst6I	CTCTTC	3 cut(s)	82, 146, 216
BstACI	GRCGYC	1 cut(s)	112
BstAFI	CTTAAG	1 cut(s)	194
BstAPI	GCANNNNNTGC	1 cut(s)	689
BstBAI	YACGTR	2 cut(s)	555, 816
BstC8I	GCNNGC	1 cut(s)	161
BstDEI	CTNAG	1 cut(s)	905
BstDSI	CCRYGG	1 cut(s)	785
BstF5I	GGATG	3 cut(s)	117, 672, 917
BstFNI	CGCG	1 cut(s)	218
BstH2I	RGCGCY	1 cut(s)	115
BstHHI	GCGC	3 cut(s)	114, 283, 504
BstKTI	GATC	5 cut(s)	363, 603, 782, 917, 970
BstMAI	GTCTC	1 cut(s)	947
BstMBI	GATC	5 cut(s)	360, 600, 779, 914, 967
BstMWI	GCNNNNNNNGC	4 cut(s)	407, 443, 452, 689
BstNI	CCWGG	1 cut(s)	291
BstNSI	RCATGY	1 cut(s)	646
BstSCI	CCNGG	3 cut(s)	186, 289, 535
BstSFI	CTRYAG	2 cut(s)	157, 709
BstSLI	GKGCMC	1 cut(s)	821
BstUI	CGCG	1 cut(s)	218
BstV1I	GCAGC	4 cut(s)	143, 290, 449, 464
BstX2I	RGATCY	1 cut(s)	967
BstYI	RGATCY	1 cut(s)	967
BsuRI	GGCC	5 cut(s)	204, 294, 401, 750, 843
BtgI	CCRYGG	1 cut(s)	785
BtgZI	GCGATG	1 cut(s)	789
BtrI	CACGTC	1 cut(s)	310
BtsCI	GGATG	3 cut(s)	117, 672, 917
BtsI	GCAGTG	3 cut(s)	53, 546, 718
BtsIMutI	CAGTG	4 cut(s)	53, 319, 546, 718
BveI	ACCTGC	1 cut(s)	421
Cac8I	GCNNGC	1 cut(s)	161
CaiI	CAGNNNCTG	1 cut(s)	799
CciI	TCATGA	1 cut(s)	492
CfoI	GCGC	3 cut(s)	114, 283, 504
Cfr13I	GGNCC	3 cut(s)	190, 832, 841
CseI	GACGC	1 cut(s)	224
Csp6I	GTAC	5 cut(s)	241, 556, 745, 804, 813
CviAII	CATG	7 cut(s)	15, 95, 355, 397, 493, 643, 730
CviQI	GTAC	5 cut(s)	241, 556, 745, 804, 813
DdeI	CTNAG	1 cut(s)	905
DinI	GGCGCC	1 cut(s)	113
DpnI	GATC	5 cut(s)	362, 602, 781, 916, 969
DpnII	GATC	5 cut(s)	360, 600, 779, 914, 967
EaeI	YGGCCR	4 cut(s)	202, 292, 399, 748
Eam1104I	CTCTTC	3 cut(s)	82, 146, 216
EarI	CTCTTC	3 cut(s)	82, 146, 216
EciI	GGCGGA	1 cut(s)	845
Eco130I	CCWWGG	1 cut(s)	662
Eco47I	GGWCC	2 cut(s)	190, 832
Eco57I	CTGAAG	3 cut(s)	78, 356, 984
EcoRII	CCWGG	1 cut(s)	289
EcoT14I	CCWWGG	1 cut(s)	662
EgeI	GGCGCC	1 cut(s)	113
EheI	GGCGCC	1 cut(s)	113
ErhI	CCWWGG	1 cut(s)	662
FaeI	CATG	7 cut(s)	18, 98, 358, 400, 496, 646, 733
FaiI	YATR	9 cut(s)	16, 96, 356, 398, 494, 522, 644, 731, 926
FatI	CATG	7 cut(s)	14, 94, 354, 396, 492, 642, 729
Fnu4HI	GCNGC	7 cut(s)	157, 279, 295, 428, 453, 463, 828
FokI	GGATG	3 cut(s)	104, 679, 904
Fsp4HI	GCNGC	7 cut(s)	157, 279, 295, 428, 453, 463, 828
FspBI	CTAG	2 cut(s)	419, 618
GlaI	GCGC	3 cut(s)	113, 282, 503
GluI	GCNGC	7 cut(s)	157, 279, 295, 428, 453, 463, 828
GsuI	CTGGAG	1 cut(s)	881
HaeII	RGCGCY	1 cut(s)	115
HaeIII	GGCC	5 cut(s)	204, 294, 401, 750, 843
HapII	CCGG	4 cut(s)	188, 536, 638, 807
HgaI	GACGC	1 cut(s)	224
HhaI	GCGC	3 cut(s)	114, 283, 504
Hin1I	GRCGYC	1 cut(s)	112
Hin1II	CATG	7 cut(s)	18, 98, 358, 400, 496, 646, 733
Hin6I	GCGC	3 cut(s)	112, 281, 502
HinP1I	GCGC	3 cut(s)	112, 281, 502
HincII	GTYRAC	1 cut(s)	648
HindII	GTYRAC	1 cut(s)	648
HinfI	GANTC	5 cut(s)	10, 52, 796, 884, 976
HpaI	GTTAAC	1 cut(s)	648
HpaII	CCGG	4 cut(s)	188, 536, 638, 807
HphI	GGTGA	1 cut(s)	431
Hpy166II	GTNNAC	5 cut(s)	51, 137, 648, 804, 819
Hpy188I	TCNGA	6 cut(s)	9, 214, 889, 937, 964, 981
Hpy188III	TCNNGA	4 cut(s)	56, 493, 604, 735
Hpy8I	GTNNAC	5 cut(s)	51, 137, 648, 804, 819
Hpy99I	CGWCG	2 cut(s)	218, 311
HpyAV	CCTTC	3 cut(s)	19, 191, 764
HpyCH4III	ACNGT	1 cut(s)	592
HpyCH4IV	ACGT	6 cut(s)	33, 166, 309, 554, 758, 815
HpyCH4V	TGCA	6 cut(s)	159, 278, 410, 628, 711, 819
HpyF10VI	GCNNNNNNNGC	4 cut(s)	407, 443, 452, 689
HpyF3I	CTNAG	1 cut(s)	905
HpySE526I	ACGT	6 cut(s)	33, 166, 309, 554, 758, 815
Hsp92I	GRCGYC	1 cut(s)	112
Hsp92II	CATG	7 cut(s)	18, 98, 358, 400, 496, 646, 733
HspAI	GCGC	3 cut(s)	112, 281, 502
KasI	GGCGCC	1 cut(s)	111
KspAI	GTTAAC	1 cut(s)	648
Kzo9I	GATC	5 cut(s)	360, 600, 779, 914, 967
LguI	GCTCTTC	2 cut(s)	146, 216
LmnI	GCTCC	4 cut(s)	486, 680, 789, 901
Lsp1109I	GCAGC	4 cut(s)	143, 290, 449, 464
LweI	GCATC	1 cut(s)	513
MaeI	CTAG	2 cut(s)	419, 618
MaeII	ACGT	6 cut(s)	33, 166, 309, 554, 758, 815
MaeIII	GTNAC	1 cut(s)	370
MalI	GATC	5 cut(s)	362, 602, 781, 916, 969
MboI	GATC	5 cut(s)	360, 600, 779, 914, 967
MfeI	CAATTG	1 cut(s)	231
MflI	RGATCY	1 cut(s)	967
MhlI	GDGCHC	1 cut(s)	821
MluCI	AATT	3 cut(s)	106, 206, 231
Mly113I	GGCGCC	1 cut(s)	112
MlyI	GAGTC	3 cut(s)	46, 805, 893
MmeI	TCCRAC	4 cut(s)	149, 237, 554, 945
MseI	TTAA	5 cut(s)	195, 468, 561, 647, 988
MspCI	CTTAAG	1 cut(s)	194
MspI	CCGG	4 cut(s)	188, 536, 638, 807
MspR9I	CCNGG	3 cut(s)	188, 291, 537
MunI	CAATTG	1 cut(s)	231
MvaI	CCWGG	1 cut(s)	291
MvnI	CGCG	1 cut(s)	218
MwoI	GCNNNNNNNGC	4 cut(s)	407, 443, 452, 689
NarI	GGCGCC	1 cut(s)	112
NciI	CCSGG	2 cut(s)	188, 537
NdeII	GATC	5 cut(s)	360, 600, 779, 914, 967
NlaIII	CATG	7 cut(s)	18, 98, 358, 400, 496, 646, 733
NlaIV	GGNNCC	2 cut(s)	113, 791
NmeAIII	GCCGAG	2 cut(s)	427, 438
NmuCI	GTSAC	1 cut(s)	370
NspI	RCATGY	1 cut(s)	646
PagI	TCATGA	1 cut(s)	492
PaqCI	CACCTGC	1 cut(s)	421
PciI	ACATGT	1 cut(s)	642
PciSI	GCTCTTC	2 cut(s)	146, 216
PfeI	GAWTC	2 cut(s)	10, 976
PflFI	GACNNNGTC	2 cut(s)	368, 590
PkrI	GCNGC	7 cut(s)	158, 280, 296, 429, 454, 464, 829
PleI	GAGTC	3 cut(s)	46, 804, 892
PluTI	GGCGCC	1 cut(s)	115
PpsI	GAGTC	3 cut(s)	46, 804, 892
Ppu21I	YACGTR	2 cut(s)	555, 816
PscI	ACATGT	1 cut(s)	642
Psp6I	CCWGG	1 cut(s)	289
PspGI	CCWGG	1 cut(s)	289
PspN4I	GGNNCC	2 cut(s)	113, 791
PspPI	GGNCC	3 cut(s)	190, 832, 841
PstI	CTGCAG	2 cut(s)	161, 713
PstNI	CAGNNNCTG	1 cut(s)	799
PsuI	RGATCY	1 cut(s)	967
PsyI	GACNNNGTC	2 cut(s)	368, 590
RsaI	GTAC	5 cut(s)	242, 557, 746, 805, 814
RsaNI	GTAC	5 cut(s)	241, 556, 745, 804, 813
SapI	GCTCTTC	2 cut(s)	146, 216
SaqAI	TTAA	5 cut(s)	195, 468, 561, 647, 988
SatI	GCNGC	7 cut(s)	157, 279, 295, 428, 453, 463, 828
Sau3AI	GATC	5 cut(s)	360, 600, 779, 914, 967
Sau96I	GGNCC	3 cut(s)	190, 832, 841
SchI	GAGTC	3 cut(s)	46, 805, 893
ScrFI	CCNGG	3 cut(s)	188, 291, 537
SduI	GDGCHC	1 cut(s)	821
SfaNI	GCATC	1 cut(s)	513
SfcI	CTRYAG	2 cut(s)	157, 709
SfoI	GGCGCC	1 cut(s)	113
SinI	GGWCC	2 cut(s)	190, 832
SmlI	CTYRAG	1 cut(s)	194
SmoI	CTYRAG	1 cut(s)	194
Sse9I	AATT	3 cut(s)	106, 206, 231
SsiI	CCGC	6 cut(s)	218, 295, 425, 428, 827, 830
SspDI	GGCGCC	1 cut(s)	111
SspMI	CTAG	2 cut(s)	419, 618
StyD4I	CCNGG	3 cut(s)	186, 289, 535
StyI	CCWWGG	1 cut(s)	662
TaaI	ACNGT	1 cut(s)	592
TaiI	ACGT	6 cut(s)	36, 169, 312, 557, 761, 818
TaqI	TCGA	3 cut(s)	363, 507, 585
TasI	AATT	3 cut(s)	106, 206, 231
TauI	GCSGC	3 cut(s)	297, 430, 830
TfiI	GAWTC	2 cut(s)	10, 976
Tru1I	TTAA	5 cut(s)	195, 468, 561, 647, 988
Tru9I	TTAA	5 cut(s)	195, 468, 561, 647, 988
TscAI	CASTG	4 cut(s)	53, 326, 553, 718
TseFI	GTSAC	1 cut(s)	370
TseI	GCWGC	4 cut(s)	156, 278, 452, 462
Tsp45I	GTSAC	1 cut(s)	370
TspDTI	ATGAA	2 cut(s)	509, 989
TspGWI	ACGGA	3 cut(s)	238, 891, 927
TspRI	CASTG	4 cut(s)	53, 326, 553, 718
Tth111I	GACNNNGTC	2 cut(s)	368, 590
Vha464I	CTTAAG	1 cut(s)	194
VneI	GTGCAC	1 cut(s)	817
VpaK11BI	GGWCC	2 cut(s)	190, 832
XceI	RCATGY	1 cut(s)	646
XcmI	CCANNNNNNNNNTGG	2 cut(s)	251, 758
XspI	CTAG	2 cut(s)	419, 618

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.