Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0018510

Physical Location & Seq

Genomic Coordinates Forward (+)

60577291 .. 60582038

Gene Structure 4748 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0450700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2013 bp

ATGGATCCCGAAATTAATCTCGGGGAGGTTACAATCACCAAAGCGACCGGAAAACTAAACGAGTCGCCGCCGGAGAACGGGATTGTATCAGAAACTCCCAGAGAGAAGAAAACAAATGATAATGGCGTAGCCAAGGTTAAAATTGGGAATGGAGCGGCAGAAGCAAACCTGGAGAACGGCGACGACGACGTCTTTTTTACTCCAATGGCTCCCCCGGAAGAGTCGGAGCACGTGAAATTAGCCGTTACGCCCAAATTAACAGAAGAGGCGGAACCAGAAGAACAAGAGGAAGCAGGGATCCTCCAACCTCATGCAAAGCTCCCGAAGCCCGAGGCACCGCCTGGCCTCCATGACCCTCCTCCTTCCCCCAACGACGACGTTTCGGACAACAACCAGAAATTCGGCGTGGACATGCCGGCGATAGGGAAGCTCATCCGGAAGAGAAGCAACGATCTCTCCTCGGCATTCCTGAAGACGCTATCGTCGCTCAAGGACAACAATTTCGCCGACGAGTTTCTGAAGCCGAATGTAACCGAGTTCCATCTCTCCGGCCTCAAAGTGACGGTGAAGCACAAGAGCGAGACGGAGCAGCAAACGCAGGGTCTCAAAGGTCGGATCACCTTCTTCTCCAGGTCCAATTGCCGGGACTGCACGGCGGTCCGGAGCTTTTTCAAAGAGAAAGGACTCAGATTCGTCGAGATAAACGTCGACGTTTATCCTCAGAGAGAGCAAGAGCTGATCGAGAGGACCGGTACGTCGTCGGTGCCTCAGGTTTTCTTCAACGAGAAGCTATTCGGCGGTTTGGTGGCGCTGAATTCGCTGAGGAACAGCGGCGAGTTTGACCAGCGGTTGAAGGAAATGATCGGGTCGAAATGCCCCGACGACGCGCCCGCTTCGCCTGTTTACGGGTTTGATGATCTCGAGGAGGAATTGACGGACCAGATGATAGGGATCGTGAGGGTGTTGAGGCTGAGATTGCCGATTCAGGACCGTCTGATGAAGATGAAGATCGTGAAGAACTGCTTCGCCGGCGGCGAGATGGTGGAGGTCCTCATTCAGCAGTTGGACTGTGGCCGTAAACAGGCGGTTGAGATTGGAAGGCAACTGGCAAGGAGGCACTTCATTCATCACGTCTTTGGAGAAAATGAGTTTGAAGATGGAAACCATTTTTACCGATTCCTTGAGCATGAACCATTTATCCCCAAATGCTATAATTTCCGAGGATCCATAAATGACTGTGAGCCCAAGCCTGCAGCTGAAGTTGGCCAAAGGCTCACCAAGATCATGTCTGCCATACTTGAGTCCTATGCCTCTGATGACAGGTGCCTACTTGATTACATTGGCATAAGCAACAGTGAGGAATTCCGAAGGTACATAAATTTGGTTCAAGAGCTTCACCGAGTAAACCTTTTTGAACTCTCGCCAAATGAGAGGCTAGCCTTCTTCTTGAACCTGTACAATGCCATGGTCATACATGCAGTTATCAGGGTAGGGCGTCCAAAAGGTGTCATTGAAAGGAGATCCTTTTACAATGACTTCCAGTATATAGTTGGAGGTTATTCTTATTCCCTTAGTAACATCGAAAATGGCATCCTTAGAAACAACAGGAGACCTCCTTATTCCCTAGTCAAGCCATTTGGCACTGGAGACAGCCGCATAGAGCTTGCTTGTGCTAAAGTGAATCCAATAATCCACTTTGGACTGTGCAATGGGACAAGGTCGAGCCCGACAGTAAGGTTCTTCTCACCCCAAGTAATCGAAGCTGAATTAAGATCTGCAGCAAGAGAGTTCTTTAAGAGTGGTGGAATGGAGGTGAACTTGGAGAAGAGGACTGTGTATCTCACCCAAATCATCAAATGGTTCAATAATGATTTTGGACAAGATAAAGACATTTTAGAGTGGATCCTCAATTACTTGGATCCAACTAGAGCAGGTCTTTTGACACATTTGTTGGGTGATGGAGGACCTATACATATTTCTTACCAGAACTATGATTGGTCCATGAACTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003825 GO:0005975 GO:0005984 GO:0005991 GO:0005992 GO:0006793 GO:0006796 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008194 GO:0009058 GO:0009311 GO:0009312 GO:0009743 GO:0009756 GO:0009790 GO:0009791 GO:0009793 GO:0009832 GO:0009987 GO:0010033 GO:0010154 GO:0010182 GO:0016051 GO:0016311 GO:0016740 GO:0016757 GO:0016758 GO:0016787 GO:0016788 GO:0016791 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0033554 GO:0034637 GO:0035251 GO:0042221 GO:0042546 GO:0042578 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0046351 GO:0046527 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051301 GO:0051716 GO:0061458 GO:0065007 GO:0070413 GO:0070887 GO:0071310 GO:0071322 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_transf_20 Trehalose_PPase

Protein Analysis

670

Amino Acids

75.8

Weight (kDa)

5.68

Isoelectric Point (pI)

47.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glutaredoxin	PF00462	206 - 264	2.8e-15	Glutaredoxin
DEP	PF00610	326 - 393	3.6e-19	Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP)
DUF547	PF04784	472 - 597	4.7e-36	Protein of unknown function, DUF547

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018510)

Species	Orthologous Gene IDs
malus_domestica	MD17G1261200.v1.1
rosa_multiflora	Rmu_co8406561.1_g000001 Rmu_sc0018109.1_g000006
rosa_roxburghii	Rroxscaffold_1G00004500 Rroxscaffold_5G00359800
rosa_rugosa	Rorug04G0450700
rosa_samantha	Rh4BG196600 Rh4DG197200 Rh7CG141000 Rh7DG138700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	188, 481, 754
AatII	GACGTC	1 cut(s)	192
Acc36I	ACCTGC	1 cut(s)	1922
AccB1I	GGYRCC	3 cut(s)	334, 763, 1323
AccBSI	CCGCTC	1 cut(s)	155
AccI	GTMKAC	1 cut(s)	708
AccII	CGCG	1 cut(s)	887
AccIII	TCCGGA	2 cut(s)	435, 660
AcoI	YGGCCR	2 cut(s)	1072, 1264
AcsI	RAATTY	4 cut(s)	398, 814, 1361, 1378
AcuI	CTGAAG	3 cut(s)	491, 539, 1278
AcvI	CACGTG	1 cut(s)	232
AcyI	GRCGYC	2 cut(s)	189, 1495
AfaI	GTAC	3 cut(s)	754, 1373, 1457
AfiI	CCNNNNNNNGG	5 cut(s)	77, 422, 642, 905, 1081
AgeI	ACCGGT	1 cut(s)	749
AgsI	TTSAA	9 cut(s)	673, 781, 853, 1154, 1388, 1415, 1450, 1514, 1864
AjiI	CACGTC	1 cut(s)	1132
AjnI	CCWGG	3 cut(s)	168, 340, 629
AloI	GAACNNNNNNTCC	2 cut(s)	1182, 1214
AluBI	AGCT	9 cut(s)	319, 430, 666, 736, 790, 1256, 1393, 1663, 1763
AluI	AGCT	9 cut(s)	319, 430, 666, 736, 790, 1256, 1393, 1663, 1763
Alw21I	GWGCWC	1 cut(s)	231
Alw26I	GTCTC	4 cut(s)	575, 608, 1603, 1641
Ama87I	CYCGRG	3 cut(s)	20, 329, 920
Aor13HI	TCCGGA	2 cut(s)	435, 660
AoxI	GGCC	4 cut(s)	343, 550, 1072, 1264
ApeKI	GCWGC	3 cut(s)	589, 1253, 1778
ApoI	RAATTY	4 cut(s)	398, 814, 1361, 1378
ArsI	GACNNNNNNTTYG	4 cut(s)	485, 517, 586, 618
AseI	ATTAAT	1 cut(s)	15
AsiGI	ACCGGT	1 cut(s)	749
Asp700I	GAANNNNTTC	2 cut(s)	791, 1022
AspLEI	GCGC	2 cut(s)	811, 889
AspS9I	GGNCC	8 cut(s)	633, 658, 747, 937, 988, 1048, 1964, 1998
AsuC2I	CCSGG	2 cut(s)	215, 644
AsuHPI	GGTGA	9 cut(s)	28, 577, 610, 1267, 1388, 1737, 1825, 1834, 1967
AsuNHI	GCTAGC	1 cut(s)	1435
AvaI	CYCGRG	3 cut(s)	20, 329, 920
AvaII	GGWCC	8 cut(s)	633, 658, 747, 937, 988, 1048, 1964, 1998
AxyI	CCTNAGG	1 cut(s)	768
BalI	TGGCCA	1 cut(s)	1266
BamHI	GGATCC	5 cut(s)	4, 297, 1223, 1902, 1918
BanI	GGYRCC	3 cut(s)	334, 763, 1323
BanII	GRGCYC	2 cut(s)	1245, 1727
BbrPI	CACGTG	1 cut(s)	232
BbsI	GAAGAC	1 cut(s)	479
Bbv12I	GWGCWC	1 cut(s)	231
BbvCI	CCTCAGC	1 cut(s)	821
BbvI	GCAGC	3 cut(s)	601, 1265, 1790
BccI	CCATC	4 cut(s)	549, 1033, 1151, 1952
BceAI	ACGGC	4 cut(s)	193, 227, 669, 1059
BciT130I	CCWGG	3 cut(s)	170, 342, 631
BcnI	CCSGG	2 cut(s)	215, 644
BcoDI	GTCTC	4 cut(s)	575, 608, 1603, 1641
BfaI	CTAG	3 cut(s)	1436, 1625, 1926
BfmI	CTRYAG	2 cut(s)	1251, 1776
BfoI	RGCGCY	1 cut(s)	812
BfuAI	ACCTGC	1 cut(s)	1922
BglII	AGATCT	1 cut(s)	1772
BisI	GCNGC	8 cut(s)	68, 156, 590, 832, 1033, 1254, 1654, 1779
BlsI	GCNGC	8 cut(s)	69, 157, 591, 833, 1034, 1255, 1655, 1780
Bme1390I	CCNGG	5 cut(s)	170, 215, 342, 631, 644
Bme18I	GGWCC	8 cut(s)	633, 658, 747, 937, 988, 1048, 1964, 1998
BmeT110I	CYCGRG	3 cut(s)	20, 329, 920
BmgBI	CACGTC	1 cut(s)	1132
BmgT120I	GGNCC	8 cut(s)	633, 658, 747, 937, 988, 1048, 1964, 1998
BmrFI	CCNGG	5 cut(s)	170, 215, 342, 631, 644
BmsI	GCATC	1 cut(s)	1599
BmtI	GCTAGC	1 cut(s)	1439
BpiI	GAAGAC	1 cut(s)	479
BpmI	CTGGAG	3 cut(s)	191, 613, 1665
Bpu10I	CCTNAGC	1 cut(s)	821
BpuEI	CTTGAG	3 cut(s)	473, 1202, 1319
BpuMI	CCSGG	2 cut(s)	215, 644
BsaAI	YACGTR	1 cut(s)	232
BsaBI	GATNNNNATC	2 cut(s)	950, 1007
BsaHI	GRCGYC	2 cut(s)	189, 1495
BsaI	GGTCTC	2 cut(s)	608, 1603
BsaJI	CCNNGG	6 cut(s)	132, 213, 330, 459, 1219, 1464
BsaWI	WCCGGW	4 cut(s)	47, 435, 660, 749
BsaXI	ACNNNNNCTCC	2 cut(s)	440, 470
Bsc4I	CCNNNNNNNGG	5 cut(s)	77, 422, 642, 905, 1081
Bse118I	RCCGGY	3 cut(s)	415, 749, 1028
Bse1I	ACTGG	3 cut(s)	1110, 1540, 1648
Bse21I	CCTNAGG	1 cut(s)	768
Bse3DI	GCAATG	1 cut(s)	1714
Bse8I	GATNNNNATC	2 cut(s)	950, 1007
BseAI	TCCGGA	2 cut(s)	435, 660
BseBI	CCWGG	3 cut(s)	170, 342, 631
BseDI	CCNNGG	6 cut(s)	132, 213, 330, 459, 1219, 1464
BseGI	GGATG	2 cut(s)	432, 1590
BseJI	GATNNNNATC	2 cut(s)	950, 1007
BseLI	CCNNNNNNNGG	5 cut(s)	77, 422, 642, 905, 1081
BseMI	GCAATG	1 cut(s)	1714
BseMII	CTCAG	5 cut(s)	700, 734, 782, 812, 962
BseNI	ACTGG	3 cut(s)	1110, 1540, 1648
BseRI	GAGGAG	3 cut(s)	348, 448, 938
BseXI	GCAGC	3 cut(s)	601, 1265, 1790
BsgI	GTGCAG	1 cut(s)	634
Bsh1236I	CGCG	1 cut(s)	887
Bsh1285I	CGRYCG	1 cut(s)	48
BshFI	GGCC	4 cut(s)	345, 552, 1074, 1266
BshNI	GGYRCC	3 cut(s)	334, 763, 1323
BshTI	ACCGGT	1 cut(s)	749
BsiEI	CGRYCG	1 cut(s)	48
BsiHKAI	GWGCWC	1 cut(s)	231
BsiHKCI	CYCGRG	3 cut(s)	20, 329, 920
BslFI	GGGAC	2 cut(s)	659, 1726
BslI	CCNNNNNNNGG	5 cut(s)	77, 422, 642, 905, 1081
BsmAI	GTCTC	4 cut(s)	575, 608, 1603, 1641
BsmBI	CGTCTC	1 cut(s)	575
BsmFI	GGGAC	2 cut(s)	659, 1726
BsmI	GAATGC	1 cut(s)	464
BsnI	GGCC	4 cut(s)	345, 552, 1074, 1266
Bso31I	GGTCTC	2 cut(s)	608, 1603
BsoBI	CYCGRG	3 cut(s)	20, 329, 920
Bsp1286I	GDGCHC	3 cut(s)	231, 1245, 1727
Bsp13I	TCCGGA	2 cut(s)	435, 660
Bsp1407I	TGTACA	1 cut(s)	1455
Bsp19I	CCATGG	1 cut(s)	1464
BspANI	GGCC	4 cut(s)	345, 552, 1074, 1266
BspCNI	CTCAG	5 cut(s)	699, 733, 781, 813, 963
BspEI	TCCGGA	2 cut(s)	435, 660
BspFNI	CGCG	1 cut(s)	887
BspMAI	CTGCAG	2 cut(s)	1255, 1780
BspMI	ACCTGC	1 cut(s)	1922
BspOI	GCTAGC	1 cut(s)	1439
BspT107I	GGYRCC	3 cut(s)	334, 763, 1323
BspTNI	GGTCTC	2 cut(s)	608, 1603
BsrBI	CCGCTC	1 cut(s)	155
BsrDI	GCAATG	1 cut(s)	1714
BsrFI	RCCGGY	3 cut(s)	415, 749, 1028
BsrGI	TGTACA	1 cut(s)	1455
BsrI	ACTGG	3 cut(s)	1110, 1540, 1648
BssAI	RCCGGY	3 cut(s)	415, 749, 1028
BssECI	CCNNGG	6 cut(s)	132, 213, 330, 459, 1219, 1464
BssNI	GRCGYC	2 cut(s)	189, 1495
BssT1I	CCWWGG	2 cut(s)	132, 1464
Bst2UI	CCWGG	3 cut(s)	170, 342, 631
Bst4CI	ACNGT	8 cut(s)	565, 992, 1070, 1238, 1355, 1704, 1732, 1834
Bst6I	CTCTTC	4 cut(s)	213, 258, 434, 1820
BstACI	GRCGYC	2 cut(s)	189, 1495
BstAUI	TGTACA	1 cut(s)	1455
BstBAI	YACGTR	1 cut(s)	232
BstC8I	GCNNGC	6 cut(s)	417, 891, 1030, 1251, 1437, 1665
BstDEI	CTNAG	7 cut(s)	686, 720, 768, 821, 971, 1571, 1595
BstDSI	CCRYGG	1 cut(s)	1464
BstF5I	GGATG	2 cut(s)	432, 1590
BstFNI	CGCG	1 cut(s)	887
BstH2I	RGCGCY	1 cut(s)	812
BstHHI	GCGC	2 cut(s)	811, 889
BstMAI	GTCTC	4 cut(s)	575, 608, 1603, 1641
BstMCI	CGRYCG	1 cut(s)	48
BstMWI	GCNNNNNNNGC	9 cut(s)	161, 325, 484, 595, 648, 817, 895, 976, 1029
BstNI	CCWGG	3 cut(s)	170, 342, 631
BstNSI	RCATGY	2 cut(s)	415, 1478
BstSCI	CCNGG	5 cut(s)	168, 213, 340, 629, 642
BstSFI	CTRYAG	2 cut(s)	1251, 1776
BstUI	CGCG	1 cut(s)	887
BstV1I	GCAGC	3 cut(s)	601, 1265, 1790
BstV2I	GAAGAC	1 cut(s)	479
BstX2I	RGATCY	7 cut(s)	4, 297, 1223, 1520, 1772, 1902, 1918
BstYI	RGATCY	7 cut(s)	4, 297, 1223, 1520, 1772, 1902, 1918
Bsu36I	CCTNAGG	1 cut(s)	768
BsuRI	GGCC	4 cut(s)	345, 552, 1074, 1266
BtgI	CCRYGG	1 cut(s)	1464
BtrI	CACGTC	1 cut(s)	1132
BtsCI	GGATG	2 cut(s)	432, 1590
BtsIMutI	CAGTG	2 cut(s)	1360, 1641
BveI	ACCTGC	1 cut(s)	1922
Cac8I	GCNNGC	6 cut(s)	417, 891, 1030, 1251, 1437, 1665
CfoI	GCGC	2 cut(s)	811, 889
Cfr10I	RCCGGY	3 cut(s)	415, 749, 1028
Cfr13I	GGNCC	8 cut(s)	633, 658, 747, 937, 988, 1048, 1964, 1998
CpoI	CGGWCCG	1 cut(s)	658
CseI	GACGC	3 cut(s)	484, 893, 1484
Csp6I	GTAC	3 cut(s)	753, 1372, 1456
CspAI	ACCGGT	1 cut(s)	749
CspI	CGGWCCG	1 cut(s)	658
CviAII	CATG	8 cut(s)	311, 350, 412, 1187, 1285, 1465, 1475, 2002
CviQI	GTAC	3 cut(s)	753, 1372, 1456
DdeI	CTNAG	7 cut(s)	686, 720, 768, 821, 971, 1571, 1595
DrdI	GACNNNNNNGTC	3 cut(s)	188, 481, 754
DseDI	GACNNNNNNGTC	3 cut(s)	188, 481, 754
EaeI	YGGCCR	2 cut(s)	1072, 1264
Eam1104I	CTCTTC	4 cut(s)	213, 258, 434, 1820
EarI	CTCTTC	4 cut(s)	213, 258, 434, 1820
EciI	GGCGGA	1 cut(s)	284
Eco130I	CCWWGG	2 cut(s)	132, 1464
Eco24I	GRGCYC	2 cut(s)	1245, 1727
Eco31I	GGTCTC	2 cut(s)	608, 1603
Eco47I	GGWCC	8 cut(s)	633, 658, 747, 937, 988, 1048, 1964, 1998
Eco57I	CTGAAG	3 cut(s)	491, 539, 1278
Eco72I	CACGTG	1 cut(s)	232
Eco81I	CCTNAGG	1 cut(s)	768
Eco88I	CYCGRG	3 cut(s)	20, 329, 920
EcoO109I	RGGNCCY	2 cut(s)	1048, 1964
EcoRI	GAATTC	2 cut(s)	814, 1361
EcoRII	CCWGG	3 cut(s)	168, 340, 629
EcoT14I	CCWWGG	2 cut(s)	132, 1464
EcoT38I	GRGCYC	2 cut(s)	1245, 1727
ErhI	CCWWGG	2 cut(s)	132, 1464
Esp3I	CGTCTC	1 cut(s)	575
FaeI	CATG	8 cut(s)	314, 353, 415, 1190, 1288, 1468, 1478, 2005
FalI	AAGNNNNNCTT	2 cut(s)	1007, 1039
FaqI	GGGAC	2 cut(s)	659, 1726
FatI	CATG	8 cut(s)	310, 349, 411, 1186, 1284, 1464, 1474, 2001
FauI	CCCGC	1 cut(s)	898
FblI	GTMKAC	1 cut(s)	708
Fnu4HI	GCNGC	8 cut(s)	68, 156, 590, 832, 1033, 1254, 1654, 1779
FokI	GGATG	2 cut(s)	419, 1577
FriOI	GRGCYC	2 cut(s)	1245, 1727
Fsp4HI	GCNGC	8 cut(s)	68, 156, 590, 832, 1033, 1254, 1654, 1779
FspBI	CTAG	3 cut(s)	1436, 1625, 1926
GlaI	GCGC	2 cut(s)	810, 888
GluI	GCNGC	8 cut(s)	68, 156, 590, 832, 1033, 1254, 1654, 1779
GsuI	CTGGAG	3 cut(s)	191, 613, 1665
HaeII	RGCGCY	1 cut(s)	812
HaeIII	GGCC	4 cut(s)	345, 552, 1074, 1266
HgaI	GACGC	3 cut(s)	484, 893, 1484
HhaI	GCGC	2 cut(s)	811, 889
Hin1I	GRCGYC	2 cut(s)	189, 1495
Hin1II	CATG	8 cut(s)	314, 353, 415, 1190, 1288, 1468, 1478, 2005
Hin6I	GCGC	2 cut(s)	809, 887
HinP1I	GCGC	2 cut(s)	809, 887
HincII	GTYRAC	1 cut(s)	709
HindII	GTYRAC	1 cut(s)	709
HinfI	GANTC	8 cut(s)	62, 221, 684, 690, 982, 1176, 1301, 1681
HphI	GGTGA	9 cut(s)	28, 577, 610, 1267, 1388, 1737, 1825, 1834, 1967
Hpy166II	GTNNAC	6 cut(s)	409, 709, 904, 1079, 1405, 1816
Hpy8I	GTNNAC	6 cut(s)	409, 709, 904, 1079, 1405, 1816
HpyAV	CCTTC	6 cut(s)	372, 631, 847, 1092, 1362, 1450
HpyCH4III	ACNGT	8 cut(s)	565, 992, 1070, 1238, 1355, 1704, 1732, 1834
HpyCH4IV	ACGT	7 cut(s)	189, 231, 378, 705, 711, 755, 1131
HpyCH4V	TGCA	6 cut(s)	314, 651, 1253, 1478, 1707, 1778
HpyF10VI	GCNNNNNNNGC	9 cut(s)	161, 325, 484, 595, 648, 817, 895, 976, 1029
HpyF3I	CTNAG	7 cut(s)	686, 720, 768, 821, 971, 1571, 1595
HpySE526I	ACGT	7 cut(s)	189, 231, 378, 705, 711, 755, 1131
Hsp92I	GRCGYC	2 cut(s)	189, 1495
Hsp92II	CATG	8 cut(s)	314, 353, 415, 1190, 1288, 1468, 1478, 2005
HspAI	GCGC	2 cut(s)	809, 887
Kpn2I	TCCGGA	2 cut(s)	435, 660
KroI	GCCGGC	2 cut(s)	415, 1028
KroNI	GCCGGC	2 cut(s)	417, 1030
LmnI	GCTCC	6 cut(s)	152, 214, 226, 324, 586, 663
Lsp1109I	GCAGC	3 cut(s)	601, 1265, 1790
LweI	GCATC	1 cut(s)	1599
MaeI	CTAG	3 cut(s)	1436, 1625, 1926
MaeII	ACGT	7 cut(s)	189, 231, 378, 705, 711, 755, 1131
MaeIII	GTNAC	5 cut(s)	28, 244, 529, 559, 1574
MbiI	CCGCTC	1 cut(s)	155
MfeI	CAATTG	1 cut(s)	637
MflI	RGATCY	7 cut(s)	4, 297, 1223, 1520, 1772, 1902, 1918
MhlI	GDGCHC	3 cut(s)	231, 1245, 1727
MlsI	TGGCCA	1 cut(s)	1266
MluNI	TGGCCA	1 cut(s)	1266
MlyI	GAGTC	4 cut(s)	71, 230, 678, 1310
MmeI	TCCRAC	6 cut(s)	204, 328, 593, 1044, 1531, 1946
Mox20I	TGGCCA	1 cut(s)	1266
MreI	CGCCGGCG	1 cut(s)	1028
MroI	TCCGGA	2 cut(s)	435, 660
MroNI	GCCGGC	2 cut(s)	415, 1028
MroXI	GAANNNNTTC	2 cut(s)	791, 1022
MscI	TGGCCA	1 cut(s)	1266
MseI	TTAA	5 cut(s)	15, 138, 257, 1769, 1794
Msp20I	TGGCCA	1 cut(s)	1266
MspA1I	CMGCKG	3 cut(s)	831, 847, 1256
MspR9I	CCNGG	5 cut(s)	170, 215, 342, 631, 644
MunI	CAATTG	1 cut(s)	637
Mva1269I	GAATGC	1 cut(s)	464
MvaI	CCWGG	3 cut(s)	170, 342, 631
MvnI	CGCG	1 cut(s)	887
MwoI	GCNNNNNNNGC	9 cut(s)	161, 325, 484, 595, 648, 817, 895, 976, 1029
NaeI	GCCGGC	2 cut(s)	417, 1030
NciI	CCSGG	2 cut(s)	215, 644
NcoI	CCATGG	1 cut(s)	1464
NgoMIV	GCCGGC	2 cut(s)	415, 1028
NheI	GCTAGC	1 cut(s)	1435
NlaIII	CATG	8 cut(s)	314, 353, 415, 1190, 1288, 1468, 1478, 2005
NmeAIII	GCCGAG	1 cut(s)	440
NmuCI	GTSAC	1 cut(s)	559
NspI	RCATGY	2 cut(s)	415, 1478
PaeR7I	CTCGAG	1 cut(s)	920
PcsI	WCGNNNNNNNCGW	3 cut(s)	183, 702, 1032
PctI	GAATGC	1 cut(s)	464
PdiI	GCCGGC	2 cut(s)	417, 1030
PdmI	GAANNNNTTC	2 cut(s)	791, 1022
PfeI	GAWTC	4 cut(s)	690, 982, 1176, 1681
PflFI	GACNNNGTC	2 cut(s)	188, 1717
PinAI	ACCGGT	1 cut(s)	749
PkrI	GCNGC	8 cut(s)	69, 157, 591, 833, 1034, 1255, 1655, 1780
PleI	GAGTC	4 cut(s)	70, 229, 678, 1309
PmaCI	CACGTG	1 cut(s)	232
PmlI	CACGTG	1 cut(s)	232
PpsI	GAGTC	4 cut(s)	70, 229, 678, 1309
Ppu21I	YACGTR	1 cut(s)	232
PpuMI	RGGWCCY	2 cut(s)	1048, 1964
PshBI	ATTAAT	1 cut(s)	15
Psp5II	RGGWCCY	2 cut(s)	1048, 1964
Psp6I	CCWGG	3 cut(s)	168, 340, 629
PspCI	CACGTG	1 cut(s)	232
PspGI	CCWGG	3 cut(s)	168, 340, 629
PspPI	GGNCC	8 cut(s)	633, 658, 747, 937, 988, 1048, 1964, 1998
PspPPI	RGGWCCY	2 cut(s)	1048, 1964
PstI	CTGCAG	2 cut(s)	1255, 1780
PsuI	RGATCY	7 cut(s)	4, 297, 1223, 1520, 1772, 1902, 1918
PsyI	GACNNNGTC	2 cut(s)	188, 1717
PvuII	CAGCTG	1 cut(s)	1256
RsaI	GTAC	3 cut(s)	754, 1373, 1457
RsaNI	GTAC	3 cut(s)	753, 1372, 1456
Rsr2I	CGGWCCG	1 cut(s)	658
RsrII	CGGWCCG	1 cut(s)	658
SalI	GTCGAC	1 cut(s)	707
SaqAI	TTAA	5 cut(s)	15, 138, 257, 1769, 1794
SatI	GCNGC	8 cut(s)	68, 156, 590, 832, 1033, 1254, 1654, 1779
Sau96I	GGNCC	8 cut(s)	633, 658, 747, 937, 988, 1048, 1964, 1998
SchI	GAGTC	4 cut(s)	71, 230, 678, 1310
ScrFI	CCNGG	5 cut(s)	170, 215, 342, 631, 644
SduI	GDGCHC	3 cut(s)	231, 1245, 1727
SfaNI	GCATC	1 cut(s)	1599
SfcI	CTRYAG	2 cut(s)	1251, 1776
Sfr274I	CTCGAG	1 cut(s)	920
SgrAI	CRCCGGYG	1 cut(s)	1028
SgrDI	CGTCGACG	1 cut(s)	707
SinI	GGWCC	8 cut(s)	633, 658, 747, 937, 988, 1048, 1964, 1998
SlaI	CTCGAG	1 cut(s)	920
SmlI	CTYRAG	4 cut(s)	488, 920, 1181, 1298
SmoI	CTYRAG	4 cut(s)	488, 920, 1181, 1298
SspMI	CTAG	3 cut(s)	1436, 1625, 1926
StyD4I	CCNGG	5 cut(s)	168, 213, 340, 629, 642
StyI	CCWWGG	2 cut(s)	132, 1464
TaaI	ACNGT	8 cut(s)	565, 992, 1070, 1238, 1355, 1704, 1732, 1834
TaiI	ACGT	7 cut(s)	192, 234, 381, 708, 714, 758, 1134
TaqI	TCGA	8 cut(s)	696, 708, 741, 869, 921, 1581, 1721, 1758
TatI	WGTACW	1 cut(s)	1455
TauI	GCSGC	5 cut(s)	70, 158, 834, 1035, 1656
TfiI	GAWTC	4 cut(s)	690, 982, 1176, 1681
Tru1I	TTAA	5 cut(s)	15, 138, 257, 1769, 1794
Tru9I	TTAA	5 cut(s)	15, 138, 257, 1769, 1794
TscAI	CASTG	2 cut(s)	1360, 1648
TseFI	GTSAC	1 cut(s)	559
TseI	GCWGC	3 cut(s)	589, 1253, 1778
Tsp45I	GTSAC	1 cut(s)	559
TspDTI	ATGAA	5 cut(s)	1013, 1019, 1111, 1115, 1203
TspGWI	ACGGA	2 cut(s)	599, 950
TspRI	CASTG	2 cut(s)	1360, 1648
Tth111I	GACNNNGTC	2 cut(s)	188, 1717
VpaK11BI	GGWCC	8 cut(s)	633, 658, 747, 937, 988, 1048, 1964, 1998
VspI	ATTAAT	1 cut(s)	15
XapI	RAATTY	4 cut(s)	398, 814, 1361, 1378
XceI	RCATGY	2 cut(s)	415, 1478
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1547
XhoI	CTCGAG	1 cut(s)	920
XmiI	GTMKAC	1 cut(s)	708
XmnI	GAANNNNTTC	2 cut(s)	791, 1022
XspI	CTAG	3 cut(s)	1436, 1625, 1926
ZraI	GACGTC	1 cut(s)	190

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.