Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000007

Orthogroup ID

OG0017628

Physical Location & Seq

Genomic Coordinates Forward (+)

1555828 .. 1561513

Gene Structure 5686 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug07G0021200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2205 bp

ATGGTAATGGGGAGCCCCGGATTAAGCAACCGGAATAATGGCCAGCAGCGGTTAGGCATTACTGAGCCGATTTCGTTGGGCGGTCCCTCGGAGGAGGATGTGATCAAAAGCCGTGAGCTTGAGAAGTATTTGCATGATGCTGGATTGTATGAGAGTCAGGAAGAGGCTGTAAGTAGGGAAGAGGTGCTTGGAAGGCTCGATCAGATTGTGAAGATATGGGTTAAGACCATTAGCCGGGCAAAGGGTTTGAACGATGAACTGGTGCATCAAGCAAATGCTAAGATCTTCACGTTTGGTTCATATAGGCTAGGGGTTCATGGCCCTGGTGCAGATATAGACACACTCTGTGTTGGACCCAGACATGCAACTCGAGAAGAAGATTTCTTTGGTGAGCTATACAGGATGCTATCTGAAATGTCTGAAGTAACGGAGTTGCATCCTGTTCCTGATGCCCATGTTCCCATTATGCAGTTCAAGTTTAACGGAGTATCAATTGATCTTCTTTATGCAAAACTATCTCTTTATATTATTCCTGAGGACTTAGATGTATCACAAGATGCAATACTACATAGTGCAGATGAACAGACAGTTCGCAGTCTCAATGGCTGTAGAGTTACAGACCAAATTCTGCGTCTAGTCCCAAGCATTCAGAATTTCCGCACAACACTAAGATGCATGAGGTTATGGGCAAAGCGTCGTGGTGTTTATTCAAATGTTGCAGGATTTCTTGGTGGTATAAACTGGGCATTGCTTGTTGCTCGCATATGCCAACTTTACCCGAATGCATTGCCTAATATGCTAGTATCTCGGTTCTTCAGGGTCTATACTCAGTGGCGTTGGCCAAATCCAGTAATGCTCTGTGCTATCGAAGAAGGGTCTCTAGGACTTCCGGTTTGGGATCCCAGAAAAAATCCAAGGGACAGATATCATTTGATGCCTATAATTACTCCAGCTTATCCTTGCATGAACTCTAGCTACAATGTGTCCTCAAGTACTCTGCGTATCATGTTAGAGGAGTTTCAGAGGGGAAATGAAATTTGTGAGTGCTTGCAGGCTATGGAAGCAAATAAGGCTGATTGGGATACCCTTTTTGAGTCGTATGCTTTCTTTGAAGCATATAAGAATTATTTACAGATAGACATCAGTGCAGCAAATGCTGATGGCTTTAGAAAATGGAAAGGCTGGGTTGAGTCACGTCTCCGTCAACTAACATTGAAGATTGAGAGGAAAACTTATGAAATGCTGCAGTGTCACCCACACCCAGGTGGTTTTCCAGATAAATCCAGACCTTTTCATAGCTCATACTTCATGGGCCTACAACGAAAACAAGGAGTTCCAGTCAAGGAAGGTGAGCACTTTGATTTAAGTAAAGCTGTTGATGACTTCAAGCTTGCTGTCTACCAGTATACATTAAGGGAAATTGGAATGGAGATCCGTGTATCCCATGTAAAACGCAGGGAGATCCCTAATTTTGTATTTCCTGGAGGCGTTCGGCCTTCGCGCCCATCTAAAGTAAGTTGGGTTAGAAAGCGACGTTCAGAACTGGAGGTTTCTGGCAGCACTCAACAAGATAGGTCAGGTGAAGATAGAACTGTTTCAGATGGAGCAGATAGTGGTAGAAAGAGAAAATTGGAGAATGATAATGTAGAGACTGACCATGCCAAGTCTATGCGTCCCTGCGTTGGGGAAGTTTGTGAGGCTAGCCCTCCTTTTAGTAATGTCAGCCCATCCTCTGCAAAATATGAGAATAATGTAGAAGTTAGCACAGGAAAGATAGAAAGCCCAGCACAGATTCCTGATCAGAATGGTCAGGCTGAAGTATGGTCAAGGTGCAATCCATTAAATGTTGCTGGTGATGCATCAAGTTCTAAAGAAGCAGAGAAACTGGCCTTAGAGAAGATTATGGCTGGTCCATATGTTTCACATCAAGCTTTGCCAGAAGAACTGGATGAGCTCGAAGGTGATTCTGAAGACAGGCATCAAGTCAAAGACTTTTCTGGGAACATGAAAGACAGTCGCATCGAGCCTTCAATCGAGAAATCTAACAATGGAGCTGAAACTTCCACTGGTTCATATAATGGAGGCTTAGAGGAACTTGAGCCTGCTGAACTTATGGCACCATCCTTGAATGGAACCCCTGAACCTGTGGCACAGAGGAAGCCAATCATCAGGCTGAACTTCACTTCCTTGCCCAATGCTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0010087 GO:0010088 GO:0010089 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_CC_LHEQLE Myb_DNA-binding

Protein Analysis

734

Amino Acids

82.64

Weight (kDa)

5.86

Isoelectric Point (pI)

46.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAP_NTPase	PF20750	19 - 213	5.3e-78	Poly(A) polymerase nucleotidyltransferase domain
NTP_transf_2	PF01909	88 - 168	1.4e-10	Nucleotidyltransferase domain
PAP_central	PF04928	218 - 365	9.2e-68	Poly(A) polymerase central domain
PAP_RNA-bind	PF04926	369 - 427	2.3e-12	Poly(A) polymerase predicted RNA binding domain
PAP_RNA-bind	PF04926	428 - 500	5.9e-06	Poly(A) polymerase predicted RNA binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017628)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g21811 FvH4_3g29731
rosa_multiflora	Rmu_sc0000902.1_g000006
rosa_roxburghii	Rroxscaffold_1G00049460 Rroxscaffold_2G00097660
rosa_rugosa	Rorug06G0116300 Rorug07G0021200 Rorug07G0021200 Rorug07G0021200 Rorug07G0021300 Rorug07G0021400
rosa_samantha	Rh7DG326200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	2116
AccI	GTMKAC	2 cut(s)	1398, 1406
AccII	CGCG	1 cut(s)	1501
AciI	CCGC	3 cut(s)	49, 81, 658
AclWI	GGATC	4 cut(s)	893, 906, 1426, 1456
AcoI	YGGCCR	2 cut(s)	40, 839
AcsI	RAATTY	3 cut(s)	624, 652, 1035
AcuI	CTGAAG	4 cut(s)	441, 799, 1836, 1989
AdeI	CACNNNGTG	1 cut(s)	347
AfaI	GTAC	1 cut(s)	994
AfiI	CCNNNNNNNGG	5 cut(s)	234, 241, 1262, 1682, 1683
AgsI	TTSAA	8 cut(s)	250, 475, 711, 1112, 1216, 1387, 2031, 2128
AjiI	CACGTC	1 cut(s)	1196
AjnI	CCWGG	3 cut(s)	322, 1261, 1480
AjuI	GAANNNNNNNTTGG	4 cut(s)	171, 203, 369, 401
AleI	CACNNNNGTG	1 cut(s)	1263
AloI	GAACNNNNNNTCC	2 cut(s)	2168, 2200
Alw21I	GWGCWC	2 cut(s)	1356, 1956
Alw26I	GTCTC	4 cut(s)	602, 882, 1202, 1643
AlwI	GGATC	4 cut(s)	893, 906, 1426, 1456
Ama87I	CYCGRG	1 cut(s)	369
AoxI	GGCC	6 cut(s)	40, 319, 839, 1312, 1493, 1887
ApeKI	GCWGC	4 cut(s)	46, 1148, 1243, 1557
ApoI	RAATTY	3 cut(s)	624, 652, 1035
ArsI	GACNNNNNNTTYG	4 cut(s)	616, 648, 876, 908
Asp700I	GAANNNNTTC	1 cut(s)	1594
AspLEI	GCGC	1 cut(s)	1503
AspS9I	GGNCC	5 cut(s)	83, 320, 353, 1312, 1910
AsuC2I	CCSGG	2 cut(s)	18, 236
AsuHPI	GGTGA	6 cut(s)	401, 1244, 1361, 1592, 1865, 1973
AsuNHI	GCTAGC	1 cut(s)	1700
AvaI	CYCGRG	1 cut(s)	369
AvaII	GGWCC	3 cut(s)	83, 353, 1910
AxyI	CCTNAGG	1 cut(s)	534
BalI	TGGCCA	2 cut(s)	42, 841
BamHI	GGATCC	1 cut(s)	898
BanI	GGYRCC	1 cut(s)	2116
BanII	GRGCYC	2 cut(s)	17, 1956
BbsI	GAAGAC	1 cut(s)	1977
Bbv12I	GWGCWC	2 cut(s)	1356, 1956
BbvI	GCAGC	4 cut(s)	58, 1160, 1230, 1569
BccI	CCATC	5 cut(s)	1154, 1513, 1595, 1735, 2128
BceAI	ACGGC	1 cut(s)	96
BcgI	CGANNNNNNTGC	2 cut(s)	769, 803
BciT130I	CCWGG	3 cut(s)	324, 1263, 1482
BciVI	GTATCC	2 cut(s)	1075, 1450
BclI	TGATCA	2 cut(s)	102, 1798
BcnI	CCSGG	2 cut(s)	18, 236
BcoDI	GTCTC	4 cut(s)	602, 882, 1202, 1643
BfaI	CTAG	6 cut(s)	308, 635, 800, 881, 972, 1701
BfmI	CTRYAG	2 cut(s)	607, 1244
BfuI	GTATCC	2 cut(s)	1075, 1450
BglII	AGATCT	1 cut(s)	282
BisI	GCNGC	4 cut(s)	47, 1149, 1244, 1558
BlsI	GCNGC	4 cut(s)	48, 1150, 1245, 1559
BmcAI	AGTACT	1 cut(s)	994
Bme1390I	CCNGG	5 cut(s)	18, 236, 324, 1263, 1482
Bme18I	GGWCC	3 cut(s)	83, 353, 1910
BmeT110I	CYCGRG	1 cut(s)	369
BmgBI	CACGTC	1 cut(s)	1196
BmgT120I	GGNCC	5 cut(s)	83, 320, 353, 1312, 1910
BmiI	GGNNCC	6 cut(s)	14, 85, 355, 900, 2118, 2134
BmrFI	CCNGG	5 cut(s)	18, 236, 324, 1263, 1482
BmrI	ACTGGG	1 cut(s)	751
BmtI	GCTAGC	1 cut(s)	1704
BmuI	ACTGGG	1 cut(s)	751
BpiI	GAAGAC	1 cut(s)	1977
BpmI	CTGGAG	3 cut(s)	933, 1503, 1565
BpuEI	CTTGAG	3 cut(s)	140, 973, 2117
BpuMI	CCSGG	2 cut(s)	18, 236
BsaBI	GATNNNNATC	1 cut(s)	1139
BsaI	GGTCTC	1 cut(s)	882
BsaJI	CCNNGG	5 cut(s)	16, 87, 322, 914, 1261
BsaWI	WCCGGW	2 cut(s)	30, 889
BsaXI	ACNNNNNCTCC	2 cut(s)	1323, 1353
Bsc4I	CCNNNNNNNGG	5 cut(s)	234, 241, 1262, 1682, 1683
Bse1I	ACTGG	9 cut(s)	264, 746, 848, 1337, 1402, 1548, 1890, 1950, 2071
Bse21I	CCTNAGG	1 cut(s)	534
Bse3DI	GCAATG	2 cut(s)	746, 785
Bse8I	GATNNNNATC	1 cut(s)	1139
BseBI	CCWGG	3 cut(s)	324, 1263, 1482
BseDI	CCNNGG	5 cut(s)	16, 87, 322, 914, 1261
BseGI	GGATG	6 cut(s)	103, 408, 436, 1727, 1954, 2120
BseJI	GATNNNNATC	1 cut(s)	1139
BseLI	CCNNNNNNNGG	5 cut(s)	234, 241, 1262, 1682, 1683
BseMI	GCAATG	2 cut(s)	746, 785
BseMII	CTCAG	3 cut(s)	54, 525, 842
BseNI	ACTGG	9 cut(s)	264, 746, 848, 1337, 1402, 1548, 1890, 1950, 2071
BseRI	GAGGAG	2 cut(s)	107, 1028
BseXI	GCAGC	4 cut(s)	58, 1160, 1230, 1569
BseYI	CCCAGC	2 cut(s)	1182, 1783
BsgI	GTGCAG	3 cut(s)	348, 594, 1167
Bsh1236I	CGCG	1 cut(s)	1501
BshFI	GGCC	6 cut(s)	42, 321, 841, 1314, 1495, 1889
BshNI	GGYRCC	1 cut(s)	2116
BsiHKAI	GWGCWC	2 cut(s)	1356, 1956
BsiHKCI	CYCGRG	1 cut(s)	369
BsiSI	CCGG	4 cut(s)	18, 31, 235, 890
BslFI	GGGAC	4 cut(s)	69, 623, 932, 1659
BslI	CCNNNNNNNGG	5 cut(s)	234, 241, 1262, 1682, 1683
BsmAI	GTCTC	4 cut(s)	602, 882, 1202, 1643
BsmBI	CGTCTC	1 cut(s)	1202
BsmFI	GGGAC	4 cut(s)	69, 623, 932, 1659
BsmI	GAATGC	2 cut(s)	645, 787
BsnI	GGCC	6 cut(s)	42, 321, 841, 1314, 1495, 1889
Bso31I	GGTCTC	1 cut(s)	882
BsoBI	CYCGRG	1 cut(s)	369
Bsp1286I	GDGCHC	3 cut(s)	17, 1356, 1956
Bsp143I	GATC	8 cut(s)	102, 199, 282, 496, 898, 1431, 1461, 1798
BspACI	CCGC	3 cut(s)	49, 81, 658
BspANI	GGCC	6 cut(s)	42, 321, 841, 1314, 1495, 1889
BspCNI	CTCAG	3 cut(s)	55, 526, 841
BspFNI	CGCG	1 cut(s)	1501
BspLI	GGNNCC	6 cut(s)	14, 85, 355, 900, 2118, 2134
BspMAI	CTGCAG	1 cut(s)	1248
BspOI	GCTAGC	1 cut(s)	1704
BspPI	GGATC	4 cut(s)	893, 906, 1426, 1456
BspT107I	GGYRCC	1 cut(s)	2116
BspTNI	GGTCTC	1 cut(s)	882
BsrDI	GCAATG	2 cut(s)	746, 785
BsrI	ACTGG	9 cut(s)	264, 746, 848, 1337, 1402, 1548, 1890, 1950, 2071
BssECI	CCNNGG	5 cut(s)	16, 87, 322, 914, 1261
BssMI	GATC	8 cut(s)	102, 199, 282, 496, 898, 1431, 1461, 1798
BssNAI	GTATAC	1 cut(s)	1407
BssT1I	CCWWGG	1 cut(s)	914
Bst1107I	GTATAC	1 cut(s)	1407
Bst2UI	CCWGG	3 cut(s)	324, 1263, 1482
Bst4CI	ACNGT	3 cut(s)	589, 1594, 2015
Bst6I	CTCTTC	2 cut(s)	156, 174
BstAPI	GCANNNNNTGC	1 cut(s)	1154
BstC8I	GCNNGC	7 cut(s)	44, 760, 1049, 1053, 1392, 1702, 2103
BstDEI	CTNAG	8 cut(s)	63, 279, 534, 541, 668, 828, 1891, 2086
BstF5I	GGATG	6 cut(s)	103, 408, 436, 1727, 1954, 2120
BstFNI	CGCG	1 cut(s)	1501
BstHHI	GCGC	1 cut(s)	1503
BstKTI	GATC	8 cut(s)	105, 202, 285, 499, 901, 1434, 1464, 1801
BstMAI	GTCTC	4 cut(s)	602, 882, 1202, 1643
BstMBI	GATC	8 cut(s)	102, 199, 282, 496, 898, 1431, 1461, 1798
BstMWI	GCNNNNNNNGC	6 cut(s)	193, 796, 1061, 1070, 1154, 1856
BstNI	CCWGG	3 cut(s)	324, 1263, 1482
BstNSI	RCATGY	1 cut(s)	365
BstSCI	CCNGG	5 cut(s)	16, 234, 322, 1261, 1480
BstSFI	CTRYAG	2 cut(s)	607, 1244
BstUI	CGCG	1 cut(s)	1501
BstV1I	GCAGC	4 cut(s)	58, 1160, 1230, 1569
BstV2I	GAAGAC	1 cut(s)	1977
BstX2I	RGATCY	4 cut(s)	282, 898, 1431, 1461
BstYI	RGATCY	4 cut(s)	282, 898, 1431, 1461
BstZ17I	GTATAC	1 cut(s)	1407
Bsu36I	CCTNAGG	1 cut(s)	534
BsuI	GTATCC	2 cut(s)	1075, 1450
BsuRI	GGCC	6 cut(s)	42, 321, 841, 1314, 1495, 1889
BtrI	CACGTC	1 cut(s)	1196
BtsCI	GGATG	6 cut(s)	103, 408, 436, 1727, 1954, 2120
BtsI	GCAGTG	1 cut(s)	1253
BtsIMutI	CAGTG	4 cut(s)	836, 1150, 1253, 2064
Cac8I	GCNNGC	7 cut(s)	44, 760, 1049, 1053, 1392, 1702, 2103
CfoI	GCGC	1 cut(s)	1503
Cfr13I	GGNCC	5 cut(s)	83, 320, 353, 1312, 1910
CseI	GACGC	3 cut(s)	620, 683, 1661
Csp6I	GTAC	1 cut(s)	993
CviQI	GTAC	1 cut(s)	993
DdeI	CTNAG	8 cut(s)	63, 279, 534, 541, 668, 828, 1891, 2086
DpnI	GATC	8 cut(s)	104, 201, 284, 498, 900, 1433, 1463, 1800
DpnII	GATC	8 cut(s)	102, 199, 282, 496, 898, 1431, 1461, 1798
DraIII	CACNNNGTG	1 cut(s)	347
EaeI	YGGCCR	2 cut(s)	40, 839
Eam1104I	CTCTTC	2 cut(s)	156, 174
EarI	CTCTTC	2 cut(s)	156, 174
Ecl136II	GAGCTC	1 cut(s)	1954
Eco130I	CCWWGG	1 cut(s)	914
Eco24I	GRGCYC	2 cut(s)	17, 1956
Eco31I	GGTCTC	1 cut(s)	882
Eco32I	GATATC	1 cut(s)	926
Eco47I	GGWCC	3 cut(s)	83, 353, 1910
Eco53kI	GAGCTC	1 cut(s)	1954
Eco57I	CTGAAG	4 cut(s)	441, 799, 1836, 1989
Eco81I	CCTNAGG	1 cut(s)	534
Eco88I	CYCGRG	1 cut(s)	369
EcoICRI	GAGCTC	1 cut(s)	1954
EcoRII	CCWGG	3 cut(s)	322, 1261, 1480
EcoRV	GATATC	1 cut(s)	926
EcoT14I	CCWWGG	1 cut(s)	914
EcoT22I	ATGCAT	3 cut(s)	677, 787, 1861
EcoT38I	GRGCYC	2 cut(s)	17, 1956
ErhI	CCWWGG	1 cut(s)	914
Esp3I	CGTCTC	1 cut(s)	1202
FalI	AAGNNNNNCTT	2 cut(s)	171, 203
FaqI	GGGAC	4 cut(s)	69, 623, 932, 1659
FauNDI	CATATG	2 cut(s)	764, 1915
FbaI	TGATCA	2 cut(s)	102, 1798
FblI	GTMKAC	2 cut(s)	1398, 1406
Fnu4HI	GCNGC	4 cut(s)	47, 1149, 1244, 1558
FokI	GGATG	6 cut(s)	110, 415, 423, 1714, 1961, 2107
FriOI	GRGCYC	2 cut(s)	17, 1956
Fsp4HI	GCNGC	4 cut(s)	47, 1149, 1244, 1558
FspBI	CTAG	6 cut(s)	308, 635, 800, 881, 972, 1701
GlaI	GCGC	1 cut(s)	1502
GluI	GCNGC	4 cut(s)	47, 1149, 1244, 1558
GsaI	CCCAGC	2 cut(s)	1186, 1787
GsuI	CTGGAG	3 cut(s)	933, 1503, 1565
HaeIII	GGCC	6 cut(s)	42, 321, 841, 1314, 1495, 1889
HapII	CCGG	4 cut(s)	18, 31, 235, 890
HgaI	GACGC	3 cut(s)	620, 683, 1661
HhaI	GCGC	1 cut(s)	1503
Hin6I	GCGC	1 cut(s)	1501
HinP1I	GCGC	1 cut(s)	1501
HincII	GTYRAC	1 cut(s)	1205
HindII	GTYRAC	1 cut(s)	1205
HindIII	AAGCTT	2 cut(s)	1388, 1929
HinfI	GANTC	5 cut(s)	154, 1094, 1190, 1792, 1964
HpaII	CCGG	4 cut(s)	18, 31, 235, 890
HphI	GGTGA	6 cut(s)	401, 1244, 1361, 1592, 1865, 1973
Hpy166II	GTNNAC	3 cut(s)	1205, 1399, 1407
Hpy188III	TCNNGA	9 cut(s)	158, 371, 446, 533, 1274, 1284, 1796, 2035, 2202
Hpy8I	GTNNAC	3 cut(s)	1205, 1399, 1407
Hpy99I	CGWCG	2 cut(s)	699, 1536
HpyAV	CCTTC	6 cut(s)	186, 866, 1340, 1506, 1952, 2037
HpyCH4III	ACNGT	3 cut(s)	589, 1594, 2015
HpyCH4IV	ACGT	3 cut(s)	290, 1195, 1534
HpyF10VI	GCNNNNNNNGC	6 cut(s)	193, 796, 1061, 1070, 1154, 1856
HpyF3I	CTNAG	8 cut(s)	63, 279, 534, 541, 668, 828, 1891, 2086
HpySE526I	ACGT	3 cut(s)	290, 1195, 1534
HspAI	GCGC	1 cut(s)	1501
Ksp22I	TGATCA	2 cut(s)	102, 1798
Kzo9I	GATC	8 cut(s)	102, 199, 282, 496, 898, 1431, 1461, 1798
LmnI	GCTCC	3 cut(s)	12, 1604, 2051
Lsp1109I	GCAGC	4 cut(s)	58, 1160, 1230, 1569
MaeI	CTAG	6 cut(s)	308, 635, 800, 881, 972, 1701
MaeII	ACGT	3 cut(s)	290, 1195, 1534
MaeIII	GTNAC	4 cut(s)	424, 613, 1191, 1250
MalI	GATC	8 cut(s)	104, 201, 284, 498, 900, 1433, 1463, 1800
MboI	GATC	8 cut(s)	102, 199, 282, 496, 898, 1431, 1461, 1798
MfeI	CAATTG	1 cut(s)	492
MflI	RGATCY	4 cut(s)	282, 898, 1431, 1461
MhlI	GDGCHC	3 cut(s)	17, 1356, 1956
MlsI	TGGCCA	2 cut(s)	42, 841
MluCI	AATT	9 cut(s)	492, 624, 652, 942, 1035, 1123, 1419, 1468, 1628
MluNI	TGGCCA	2 cut(s)	42, 841
MlyI	GAGTC	3 cut(s)	163, 1103, 1199
MmeI	TCCRAC	1 cut(s)	331
Mox20I	TGGCCA	2 cut(s)	42, 841
Mph1103I	ATGCAT	3 cut(s)	677, 787, 1861
MroXI	GAANNNNTTC	1 cut(s)	1594
MscI	TGGCCA	2 cut(s)	42, 841
MseI	TTAA	6 cut(s)	23, 222, 480, 1364, 1412, 1841
MslI	CAYNNNNRTG	2 cut(s)	670, 1263
Msp20I	TGGCCA	2 cut(s)	42, 841
MspA1I	CMGCKG	1 cut(s)	49
MspI	CCGG	4 cut(s)	18, 31, 235, 890
MspR9I	CCNGG	5 cut(s)	18, 236, 324, 1263, 1482
MunI	CAATTG	1 cut(s)	492
Mva1269I	GAATGC	2 cut(s)	645, 787
MvaI	CCWGG	3 cut(s)	324, 1263, 1482
MvnI	CGCG	1 cut(s)	1501
MwoI	GCNNNNNNNGC	6 cut(s)	193, 796, 1061, 1070, 1154, 1856
NciI	CCSGG	2 cut(s)	18, 236
NdeI	CATATG	2 cut(s)	764, 1915
NdeII	GATC	8 cut(s)	102, 199, 282, 496, 898, 1431, 1461, 1798
NheI	GCTAGC	1 cut(s)	1700
NlaIV	GGNNCC	6 cut(s)	14, 85, 355, 900, 2118, 2134
NmuCI	GTSAC	2 cut(s)	1191, 1250
NsiI	ATGCAT	3 cut(s)	677, 787, 1861
NspI	RCATGY	1 cut(s)	365
OliI	CACNNNNGTG	1 cut(s)	1263
PaeR7I	CTCGAG	1 cut(s)	369
PctI	GAATGC	2 cut(s)	645, 787
PdmI	GAANNNNTTC	1 cut(s)	1594
PfeI	GAWTC	2 cut(s)	1792, 1964
PfoI	TCCNGGA	1 cut(s)	1480
PkrI	GCNGC	4 cut(s)	48, 1150, 1245, 1559
PleI	GAGTC	3 cut(s)	162, 1102, 1198
PpsI	GAGTC	3 cut(s)	162, 1102, 1198
Psp124BI	GAGCTC	1 cut(s)	1956
Psp6I	CCWGG	3 cut(s)	322, 1261, 1480
PspFI	CCCAGC	2 cut(s)	1182, 1783
PspGI	CCWGG	3 cut(s)	322, 1261, 1480
PspN4I	GGNNCC	6 cut(s)	14, 85, 355, 900, 2118, 2134
PspPI	GGNCC	5 cut(s)	83, 320, 353, 1312, 1910
PsrI	GAACNNNNNNTAC	2 cut(s)	959, 991
PstI	CTGCAG	1 cut(s)	1248
PsuI	RGATCY	4 cut(s)	282, 898, 1431, 1461
RsaI	GTAC	1 cut(s)	994
RsaNI	GTAC	1 cut(s)	993
RseI	CAYNNNNRTG	2 cut(s)	670, 1263
SacI	GAGCTC	1 cut(s)	1956
SaqAI	TTAA	6 cut(s)	23, 222, 480, 1364, 1412, 1841
SatI	GCNGC	4 cut(s)	47, 1149, 1244, 1558
Sau3AI	GATC	8 cut(s)	102, 199, 282, 496, 898, 1431, 1461, 1798
Sau96I	GGNCC	5 cut(s)	83, 320, 353, 1312, 1910
ScaI	AGTACT	1 cut(s)	994
SchI	GAGTC	3 cut(s)	163, 1103, 1199
ScrFI	CCNGG	5 cut(s)	18, 236, 324, 1263, 1482
SduI	GDGCHC	3 cut(s)	17, 1356, 1956
SfcI	CTRYAG	2 cut(s)	607, 1244
Sfr274I	CTCGAG	1 cut(s)	369
SinI	GGWCC	3 cut(s)	83, 353, 1910
SlaI	CTCGAG	1 cut(s)	369
SmiMI	CAYNNNNRTG	2 cut(s)	670, 1263
SmlI	CTYRAG	4 cut(s)	119, 369, 988, 2096
SmoI	CTYRAG	4 cut(s)	119, 369, 988, 2096
Sse9I	AATT	9 cut(s)	492, 624, 652, 942, 1035, 1123, 1419, 1468, 1628
SsiI	CCGC	3 cut(s)	49, 81, 658
SspMI	CTAG	6 cut(s)	308, 635, 800, 881, 972, 1701
SstI	GAGCTC	1 cut(s)	1956
StyD4I	CCNGG	5 cut(s)	16, 234, 322, 1261, 1480
StyI	CCWWGG	1 cut(s)	914
TaaI	ACNGT	3 cut(s)	589, 1594, 2015
TaiI	ACGT	3 cut(s)	293, 1198, 1537
TaqI	TCGA	6 cut(s)	198, 370, 867, 1956, 2022, 2034
TasI	AATT	9 cut(s)	492, 624, 652, 942, 1035, 1123, 1419, 1468, 1628
TatI	WGTACW	1 cut(s)	992
TfiI	GAWTC	2 cut(s)	1792, 1964
Tru1I	TTAA	6 cut(s)	23, 222, 480, 1364, 1412, 1841
Tru9I	TTAA	6 cut(s)	23, 222, 480, 1364, 1412, 1841
TscAI	CASTG	4 cut(s)	836, 1150, 1253, 2071
TseFI	GTSAC	2 cut(s)	1191, 1250
TseI	GCWGC	4 cut(s)	46, 1148, 1243, 1557
Tsp45I	GTSAC	2 cut(s)	1191, 1250
TspGWI	ACGGA	4 cut(s)	443, 498, 1190, 1424
TspRI	CASTG	4 cut(s)	836, 1150, 1253, 2071
VpaK11BI	GGWCC	3 cut(s)	83, 353, 1910
XapI	RAATTY	3 cut(s)	624, 652, 1035
XceI	RCATGY	1 cut(s)	365
XhoI	CTCGAG	1 cut(s)	369
XmiI	GTMKAC	2 cut(s)	1398, 1406
XmnI	GAANNNNTTC	1 cut(s)	1594
XspI	CTAG	6 cut(s)	308, 635, 800, 881, 972, 1701
ZrmI	AGTACT	1 cut(s)	994
Zsp2I	ATGCAT	3 cut(s)	677, 787, 1861

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.