Rmu_sc0000156.1_g000003

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000156.1
OG0019088
Physical Location & Seq
Reverse (-)
18713 .. 21214
2502 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000156.1_g000003.1.cds

Sequence Viewer

Length: 633 bp
atgaatagaggattaattggttggagatatggaaagaggagatggagagaagaaaatctagaaattttggtgaaggatggggtttcggagactgcgattttcggcgagaatgaagagagagccgaaggaaaaatgaggctaggccttcaaaccctagccaccccagccgcacaccacaaagctctcaaccctaggattccagaactggcagtgaaaccaccagaactggccggaaaacccccaaatcagctacctgaagtttctaaaccggctccgttagcctcgcctcgcttgcgcatccctatgctttctattagcctcgccatgcccttgttagactcgctatctatagtcctcacactcccctgcgcacccctgtgcctagggttagcctcacctcgcaacagccttgcagcagtccctgtgccccaatgcatcagctctggatatcacaggtgggttcgtgatgtctgccagtatcttaaggctcaaggaattctggatacaattcttgagcccagtcataacgtgcctactcctagagaagctgctactttggaagcaaatagagctaatatggaggcaaacaaggaaaaagccattattctcatgacacatgcaccatatgaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

210

Amino Acids

23.09

Weight (kDa)

8.94

Isoelectric Point (pI)

51.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 296, 370
AciI CCGC 1 cut(s) 168
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 2 cut(s) 63, 495
AcuI CTGAAG 1 cut(s) 276
AflII CTTAAG 1 cut(s) 482
AgsI TTSAA 1 cut(s) 149
AleI CACNNNNGTG 1 cut(s) 376
AluBI AGCT 5 cut(s) 182, 250, 441, 548, 572
AluI AGCT 5 cut(s) 182, 250, 441, 548, 572
Alw26I GTCTC 1 cut(s) 83
AlwNI CAGNNNCTG 1 cut(s) 422
AoxI GGCC 2 cut(s) 142, 228
ApeKI GCWGC 2 cut(s) 413, 548
ApoI RAATTY 2 cut(s) 63, 495
AseI ATTAAT 1 cut(s) 14
AspA2I CCTAGG 2 cut(s) 191, 382
AspLEI GCGC 2 cut(s) 297, 371
AsuHPI GGTGA 2 cut(s) 82, 387
AvrII CCTAGG 2 cut(s) 191, 382
BaeGI GKGCMC 1 cut(s) 429
BanII GRGCYC 1 cut(s) 519
BbvI GCAGC 2 cut(s) 425, 535
BccI CCATC 2 cut(s) 36, 71
BciVI GTATCC 1 cut(s) 496
BcoDI GTCTC 1 cut(s) 83
BfaI CTAG 6 cut(s) 59, 140, 155, 192, 383, 540
BfmI CTRYAG 1 cut(s) 348
BfrI CTTAAG 1 cut(s) 482
BfuI GTATCC 1 cut(s) 496
BisI GCNGC 3 cut(s) 168, 414, 549
BlnI CCTAGG 2 cut(s) 191, 382
BlsI GCNGC 3 cut(s) 169, 415, 550
BmiI GGNNCC 1 cut(s) 273
BmrI ACTGGG 1 cut(s) 513
BmsI GCATC 2 cut(s) 306, 444
BmuI ACTGGG 1 cut(s) 513
BpuEI CTTGAG 2 cut(s) 474, 533
BsaJI CCNNGG 2 cut(s) 191, 382
Bse118I RCCGGY 1 cut(s) 268
Bse1I ACTGG 4 cut(s) 210, 231, 475, 519
BseDI CCNNGG 2 cut(s) 191, 382
BseGI GGATG 2 cut(s) 82, 297
BseNI ACTGG 4 cut(s) 210, 231, 475, 519
BseRI GAGGAG 1 cut(s) 52
BseSI GKGCMC 1 cut(s) 429
BseXI GCAGC 2 cut(s) 425, 535
BseYI CCCAGC 1 cut(s) 163
BshFI GGCC 2 cut(s) 144, 230
BsiSI CCGG 2 cut(s) 231, 269
BslFI GGGAC 1 cut(s) 404
BsmAI GTCTC 1 cut(s) 83
BsmFI GGGAC 1 cut(s) 404
BsnI GGCC 2 cut(s) 144, 230
Bsp1286I GDGCHC 2 cut(s) 429, 519
BspACI CCGC 1 cut(s) 168
BspANI GGCC 2 cut(s) 144, 230
BspHI TCATGA 1 cut(s) 609
BspLI GGNNCC 1 cut(s) 273
BspTI CTTAAG 1 cut(s) 482
BsrFI RCCGGY 1 cut(s) 268
BsrI ACTGG 4 cut(s) 210, 231, 475, 519
BssAI RCCGGY 1 cut(s) 268
BssECI CCNNGG 2 cut(s) 191, 382
BssT1I CCWWGG 2 cut(s) 191, 382
Bst6I CTCTTC 1 cut(s) 108
BstAFI CTTAAG 1 cut(s) 482
BstC8I GCNNGC 1 cut(s) 293
BstF5I GGATG 2 cut(s) 82, 297
BstHHI GCGC 2 cut(s) 297, 371
BstMAI GTCTC 1 cut(s) 83
BstMWI GCNNNNNNNGC 4 cut(s) 164, 278, 292, 569
BstNSI RCATGY 1 cut(s) 620
BstSFI CTRYAG 1 cut(s) 348
BstSLI GKGCMC 1 cut(s) 429
BstV1I GCAGC 2 cut(s) 425, 535
BsuI GTATCC 1 cut(s) 496
BsuRI GGCC 2 cut(s) 144, 230
BtsCI GGATG 2 cut(s) 82, 297
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 1 cut(s) 216
Cac8I GCNNGC 1 cut(s) 293
CaiI CAGNNNCTG 1 cut(s) 422
CciI TCATGA 1 cut(s) 609
CfoI GCGC 2 cut(s) 297, 371
Cfr10I RCCGGY 1 cut(s) 268
CviAII CATG 3 cut(s) 325, 610, 617
EaeI YGGCCR 1 cut(s) 228
Eam1104I CTCTTC 1 cut(s) 108
EarI CTCTTC 1 cut(s) 108
Eco130I CCWWGG 2 cut(s) 191, 382
Eco147I AGGCCT 1 cut(s) 144
Eco24I GRGCYC 1 cut(s) 519
Eco32I GATATC 1 cut(s) 449
Eco57I CTGAAG 1 cut(s) 276
EcoRI GAATTC 1 cut(s) 495
EcoRV GATATC 1 cut(s) 449
EcoT14I CCWWGG 2 cut(s) 191, 382
EcoT22I ATGCAT 1 cut(s) 437
EcoT38I GRGCYC 1 cut(s) 519
ErhI CCWWGG 2 cut(s) 191, 382
FaeI CATG 3 cut(s) 328, 613, 620
FaqI GGGAC 1 cut(s) 404
FatI CATG 3 cut(s) 324, 609, 616
FauNDI CATATG 1 cut(s) 625
Fnu4HI GCNGC 3 cut(s) 168, 414, 549
FokI GGATG 2 cut(s) 89, 284
FriOI GRGCYC 1 cut(s) 519
Fsp4HI GCNGC 3 cut(s) 168, 414, 549
FspBI CTAG 6 cut(s) 59, 140, 155, 192, 383, 540
FspI TGCGCA 2 cut(s) 296, 370
GlaI GCGC 2 cut(s) 296, 370
GluI GCNGC 3 cut(s) 168, 414, 549
GsaI CCCAGC 1 cut(s) 167
HaeIII GGCC 2 cut(s) 144, 230
HapII CCGG 2 cut(s) 231, 269
HhaI GCGC 2 cut(s) 297, 371
Hin1II CATG 3 cut(s) 328, 613, 620
Hin6I GCGC 2 cut(s) 295, 369
HinP1I GCGC 2 cut(s) 295, 369
HinfI GANTC 2 cut(s) 196, 338
HpaII CCGG 2 cut(s) 231, 269
HphI GGTGA 2 cut(s) 82, 387
Hpy188I TCNGA 1 cut(s) 88
Hpy188III TCNNGA 7 cut(s) 59, 200, 444, 464, 500, 512, 610
HpyAV CCTTC 3 cut(s) 67, 119, 155
HpyCH4IV ACGT 1 cut(s) 528
HpyCH4V TGCA 3 cut(s) 413, 435, 620
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 278, 292, 569
HpySE526I ACGT 1 cut(s) 528
Hsp92II CATG 3 cut(s) 328, 613, 620
HspAI GCGC 2 cut(s) 295, 369
LmnI GCTCC 1 cut(s) 277
Lsp1109I GCAGC 2 cut(s) 425, 535
LweI GCATC 2 cut(s) 306, 444
MaeI CTAG 6 cut(s) 59, 140, 155, 192, 383, 540
MaeII ACGT 1 cut(s) 528
MboII GAAGA 2 cut(s) 62, 125
MhlI GDGCHC 2 cut(s) 429, 519
MluCI AATT 4 cut(s) 15, 63, 495, 507
MlyI GAGTC 1 cut(s) 332
MnlI CCTC 9 cut(s) 30, 129, 292, 297, 329, 365, 403, 408, 574
Mph1103I ATGCAT 1 cut(s) 437
MseI TTAA 2 cut(s) 14, 483
MslI CAYNNNNRTG 3 cut(s) 302, 376, 628
MspCI CTTAAG 1 cut(s) 482
MspI CCGG 2 cut(s) 231, 269
MwoI GCNNNNNNNGC 4 cut(s) 164, 278, 292, 569
NdeI CATATG 1 cut(s) 625
NlaIII CATG 3 cut(s) 328, 613, 620
NlaIV GGNNCC 1 cut(s) 273
NsbI TGCGCA 2 cut(s) 296, 370
NsiI ATGCAT 1 cut(s) 437
NspI RCATGY 1 cut(s) 620
OliI CACNNNNGTG 1 cut(s) 376
PagI TCATGA 1 cut(s) 609
PceI AGGCCT 1 cut(s) 144
PcsI WCGNNNNNNNCGW 1 cut(s) 92
PfeI GAWTC 1 cut(s) 196
PkrI GCNGC 3 cut(s) 169, 415, 550
PleI GAGTC 1 cut(s) 332
PpsI GAGTC 1 cut(s) 332
PshBI ATTAAT 1 cut(s) 14
PspFI CCCAGC 1 cut(s) 163
PspN4I GGNNCC 1 cut(s) 273
PstNI CAGNNNCTG 1 cut(s) 422
RseI CAYNNNNRTG 3 cut(s) 302, 376, 628
SaqAI TTAA 2 cut(s) 14, 483
SatI GCNGC 3 cut(s) 168, 414, 549
SchI GAGTC 1 cut(s) 332
SduI GDGCHC 2 cut(s) 429, 519
SetI ASST 9 cut(s) 184, 252, 256, 400, 443, 458, 531, 550, 574
SfaNI GCATC 2 cut(s) 306, 444
SfcI CTRYAG 1 cut(s) 348
SmiMI CAYNNNNRTG 3 cut(s) 302, 376, 628
SmlI CTYRAG 3 cut(s) 482, 489, 512
SmoI CTYRAG 3 cut(s) 482, 489, 512
Sse9I AATT 4 cut(s) 15, 63, 495, 507
SseBI AGGCCT 1 cut(s) 144
SsiI CCGC 1 cut(s) 168
SspMI CTAG 6 cut(s) 59, 140, 155, 192, 383, 540
StuI AGGCCT 1 cut(s) 144
StyI CCWWGG 2 cut(s) 191, 382
TaiI ACGT 1 cut(s) 531
TasI AATT 4 cut(s) 15, 63, 495, 507
TauI GCSGC 1 cut(s) 170
TfiI GAWTC 1 cut(s) 196
Tru1I TTAA 2 cut(s) 14, 483
Tru9I TTAA 2 cut(s) 14, 483
TscAI CASTG 1 cut(s) 216
TseI GCWGC 2 cut(s) 413, 548
TspDTI ATGAA 2 cut(s) 17, 126
TspGWI ACGGA 1 cut(s) 264
TspRI CASTG 1 cut(s) 216
Vha464I CTTAAG 1 cut(s) 482
VspI ATTAAT 1 cut(s) 14
XapI RAATTY 2 cut(s) 63, 495
XbaI TCTAGA 1 cut(s) 58
XceI RCATGY 1 cut(s) 620
XmaJI CCTAGG 2 cut(s) 191, 382
XspI CTAG 6 cut(s) 59, 140, 155, 192, 383, 540
Zsp2I ATGCAT 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.