MD15G1227100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0017215
Physical Location & Seq
Forward (+)
18453496 .. 18454442
947 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1227100.v1.1.491

Sequence Viewer

Length: 504 bp
ATGCTTGAATCTGCTATTTGTTTATACTTTAGTCAATGTAGTTACGTCCACATCTCGATCATGGATAAAAATGGAAAAGAAGAAATCAGTCTACCAATGCCGATTCTCCAAAGACTGGATCGTCTCGATCGTTTGCTGCAGTTCTTGGAACACAAGCATTGCCTATCAGCCAAGCATTCTTCTTGTCCTACCTCCTTGGAACCAGAAGATGATCAGTGTAAGACTTTGTCATCTGCAATTGAAGAAGCACATCACAAAGGCACACTGATGGAGAGAGTAACTATGCTTGAGAAGCGTGTTTTGCAGATAAGCCATGAGATGGATGTGGAAAATGCATCAAGTTCAAGCTGCTTCACAATTGAAGCATCTGAGGAAATTGGGCAAGGATCTTCAGAAGCATATGTGAGGAAGCCAAATTGTTGCAAGAAGGGCAGAAGAAAGAAAAATGTTGCTTCCATTATTCAGATGAGAGAATGGTTTAGATGGAGGAGAATGGGATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

168

Amino Acids

19.24

Weight (kDa)

7.58

Isoelectric Point (pI)

64.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017215)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09770 FvH4_1g09770
malus_domestica MD15G1227100.v1.1
prunus_persica Prupe.7G188200_v2.0.a1
rosa_laevigata RLG00000016661
rosa_roxburghii Rroxscaffold_2G00145420
rosa_rugosa Rorug02G0059600
rosa_samantha Rh2AG107000 Rh2BG109400 Rh2CG111200 Rh2DG110800 Rh7BG189800
rosa_wichuraiana Rw2G008290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 120
AccB7I CCANNNNNTGG 2 cut(s) 115, 319
AccI GTMKAC 1 cut(s) 91
AclWI GGATC 2 cut(s) 126, 394
AcuI CTGAAG 1 cut(s) 375
AfiI CCNNNNNNNGG 2 cut(s) 115, 319
AgsI TTSAA 4 cut(s) 8, 242, 345, 362
AluBI AGCT 1 cut(s) 348
AluI AGCT 1 cut(s) 348
Alw26I GTCTC 1 cut(s) 128
AlwI GGATC 2 cut(s) 126, 394
ApeKI GCWGC 2 cut(s) 136, 348
BbvI GCAGC 2 cut(s) 123, 335
BccI CCATC 3 cut(s) 262, 313, 477
BclI TGATCA 1 cut(s) 211
BcoDI GTCTC 1 cut(s) 128
BfmI CTRYAG 1 cut(s) 137
BisI GCNGC 2 cut(s) 137, 349
BlsI GCNGC 2 cut(s) 138, 350
BmiI GGNNCC 1 cut(s) 201
BmsI GCATC 2 cut(s) 344, 374
BpuEI CTTGAG 1 cut(s) 308
BsaJI CCNNGG 1 cut(s) 195
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 319
Bse1I ACTGG 1 cut(s) 120
Bse3DI GCAATG 1 cut(s) 157
BseDI CCNNGG 1 cut(s) 195
BseGI GGATG 2 cut(s) 328, 503
BseLI CCNNNNNNNGG 2 cut(s) 115, 319
BseMI GCAATG 1 cut(s) 157
BseMII CTCAG 1 cut(s) 360
BseNI ACTGG 1 cut(s) 120
BseRI GAGGAG 1 cut(s) 502
BseXI GCAGC 2 cut(s) 123, 335
Bsh1285I CGRYCG 1 cut(s) 130
BsiEI CGRYCG 1 cut(s) 130
BslI CCNNNNNNNGG 2 cut(s) 115, 319
BsmAI GTCTC 1 cut(s) 128
BsmBI CGTCTC 1 cut(s) 128
BsmI GAATGC 1 cut(s) 175
Bsp143I GATC 5 cut(s) 57, 118, 127, 211, 386
BspCNI CTCAG 1 cut(s) 361
BspLI GGNNCC 1 cut(s) 201
BspMAI CTGCAG 1 cut(s) 141
BspPI GGATC 2 cut(s) 126, 394
BsrDI GCAATG 1 cut(s) 157
BsrI ACTGG 1 cut(s) 120
BssECI CCNNGG 1 cut(s) 195
BssMI GATC 5 cut(s) 57, 118, 127, 211, 386
BssT1I CCWWGG 1 cut(s) 195
BstDEI CTNAG 1 cut(s) 369
BstF5I GGATG 2 cut(s) 328, 503
BstKTI GATC 5 cut(s) 60, 121, 130, 214, 389
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 5 cut(s) 57, 118, 127, 211, 386
BstMCI CGRYCG 1 cut(s) 130
BstMWI GCNNNNNNNGC 3 cut(s) 292, 301, 429
BstSFI CTRYAG 1 cut(s) 137
BstV1I GCAGC 2 cut(s) 123, 335
BstX2I RGATCY 1 cut(s) 386
BstYI RGATCY 1 cut(s) 386
BtsCI GGATG 2 cut(s) 328, 503
BtsIMutI CAGTG 2 cut(s) 221, 263
CviAII CATG 2 cut(s) 61, 314
CviJI RGCY 4 cut(s) 170, 312, 348, 412
CviKI_1 RGCY 4 cut(s) 170, 312, 348, 412
DdeI CTNAG 1 cut(s) 369
DpnI GATC 5 cut(s) 59, 120, 129, 213, 388
DpnII GATC 5 cut(s) 57, 118, 127, 211, 386
DrdI GACNNNNNNGTC 1 cut(s) 120
DseDI GACNNNNNNGTC 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 195
Eco57I CTGAAG 1 cut(s) 375
EcoT14I CCWWGG 1 cut(s) 195
EcoT22I ATGCAT 1 cut(s) 337
ErhI CCWWGG 1 cut(s) 195
Esp3I CGTCTC 1 cut(s) 128
FaeI CATG 2 cut(s) 64, 317
FaiI YATR 6 cut(s) 25, 62, 284, 315, 400, 402
FatI CATG 2 cut(s) 60, 313
FauNDI CATATG 1 cut(s) 400
FbaI TGATCA 1 cut(s) 211
FblI GTMKAC 1 cut(s) 91
Fnu4HI GCNGC 2 cut(s) 137, 349
FokI GGATG 1 cut(s) 335
Fsp4HI GCNGC 2 cut(s) 137, 349
GluI GCNGC 2 cut(s) 137, 349
Hin1II CATG 2 cut(s) 64, 317
HinfI GANTC 2 cut(s) 8, 103
Hpy166II GTNNAC 2 cut(s) 49, 92
Hpy188I TCNGA 3 cut(s) 370, 394, 465
Hpy188III TCNNGA 2 cut(s) 55, 125
Hpy8I GTNNAC 2 cut(s) 49, 92
HpyAV CCTTC 1 cut(s) 421
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 5 cut(s) 139, 236, 304, 335, 423
HpyF10VI GCNNNNNNNGC 3 cut(s) 292, 301, 429
HpyF3I CTNAG 1 cut(s) 369
HpySE526I ACGT 1 cut(s) 45
Hsp92II CATG 2 cut(s) 64, 317
Ksp22I TGATCA 1 cut(s) 211
Kzo9I GATC 5 cut(s) 57, 118, 127, 211, 386
LpnPI CCDG 2 cut(s) 101, 216
Lsp1109I GCAGC 2 cut(s) 123, 335
LweI GCATC 2 cut(s) 344, 374
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 2 cut(s) 41, 277
MalI GATC 5 cut(s) 59, 120, 129, 213, 388
MboI GATC 5 cut(s) 57, 118, 127, 211, 386
MboII GAAGA 6 cut(s) 92, 171, 218, 254, 381, 447
MfeI CAATTG 2 cut(s) 237, 357
MflI RGATCY 1 cut(s) 386
MluCI AATT 4 cut(s) 237, 357, 375, 415
MnlI CCTC 4 cut(s) 202, 364, 399, 480
Mph1103I ATGCAT 1 cut(s) 337
MseI TTAA 1 cut(s) 502
MslI CAYNNNNRTG 1 cut(s) 266
MunI CAATTG 2 cut(s) 237, 357
Mva1269I GAATGC 1 cut(s) 175
MwoI GCNNNNNNNGC 3 cut(s) 292, 301, 429
NdeI CATATG 1 cut(s) 400
NdeII GATC 5 cut(s) 57, 118, 127, 211, 386
NlaIII CATG 2 cut(s) 64, 317
NlaIV GGNNCC 1 cut(s) 201
NsiI ATGCAT 1 cut(s) 337
PcsI WCGNNNNNNNCGW 1 cut(s) 127
PctI GAATGC 1 cut(s) 175
PfeI GAWTC 2 cut(s) 8, 103
PflFI GACNNNGTC 1 cut(s) 226
PflMI CCANNNNNTGG 2 cut(s) 115, 319
PkrI GCNGC 2 cut(s) 138, 350
Ple19I CGATCG 1 cut(s) 130
PspN4I GGNNCC 1 cut(s) 201
PstI CTGCAG 1 cut(s) 141
PsuI RGATCY 1 cut(s) 386
PsyI GACNNNGTC 1 cut(s) 226
PvuI CGATCG 1 cut(s) 130
RseI CAYNNNNRTG 1 cut(s) 266
SaqAI TTAA 1 cut(s) 502
SatI GCNGC 2 cut(s) 137, 349
Sau3AI GATC 5 cut(s) 57, 118, 127, 211, 386
SetI ASST 3 cut(s) 48, 194, 350
SfaNI GCATC 2 cut(s) 344, 374
SfcI CTRYAG 1 cut(s) 137
SmiMI CAYNNNNRTG 1 cut(s) 266
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
Sse9I AATT 4 cut(s) 237, 357, 375, 415
StyI CCWWGG 1 cut(s) 195
TaiI ACGT 1 cut(s) 48
TaqI TCGA 2 cut(s) 56, 126
TasI AATT 4 cut(s) 237, 357, 375, 415
TfiI GAWTC 2 cut(s) 8, 103
Tru1I TTAA 1 cut(s) 502
Tru9I TTAA 1 cut(s) 502
TscAI CASTG 2 cut(s) 221, 270
TseI GCWGC 2 cut(s) 136, 348
TspRI CASTG 2 cut(s) 221, 270
Tth111I GACNNNGTC 1 cut(s) 226
Van91I CCANNNNNTGG 2 cut(s) 115, 319
XmiI GTMKAC 1 cut(s) 91
Zsp2I ATGCAT 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.