Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0017654

Physical Location & Seq

Genomic Coordinates Forward (+)

55281420 .. 55285075

Gene Structure 3656 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G028640.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2763 bp

ATGAGTTTTATAGAGACTGACGGTATTCCTGAATTTGCGCACGGCCTTAAAATCCTGCTGTTCGATCATGACACATTTTCCCTCATGTACACAGCATCAGTGCTTGAACTATACTCATTCAATGTTACTGCAATCGAGCAGGCATCTGTCGCTTTGTCCATGATTAGTGAACAAAAGGATCACTATGAACTAGTAATGGCTAGCATTAACATGTCTGATATTGAGAGTGTTTCTTTTCTGCATGCACTGCTCGAGAAGGATATTCCTGTCATATTGATCTCCTCTGAAATGAATGCTGACATGACCAGGAAAGCCCTAGACAAAGGAGCAAGCATTATCCTTGAAAAACCCATTCCTTTGGAGGATCTGAAAAATGTGTGGCGACACGTATACAGGAAGACAAACCCAATGGAGGACATAGGTAAACCAAATTGTGAAGAAAAGATAGACTGGCCAAAGGACTATCTAGAGTCTTCAGTGCTTAGACCTTCCTTTGCAGGTATTGAAACCAAGGAGGATGGTGCATTGTGTAGGCCTGATACAGGGCATTATTGGAATGGCACTGGCCTTGAAACTGATCTAGAAGGTTTGGATAGCTATAGCATTGAAGAGTTGTTCCCAACAAAGAGCCGAAAACAAAAAGCATCTGGGATGAAGCCAGCTGATGATACCGAAGAACAAGGAAAACAAAAAAAATTGAAACTAACTGTAGAGCAAACTGATTCTGACAGTATAGCCAGGAATCAGAAAGGGATCTCCAAGAAACATAGCAATGACAAAACAGATAAGAAAGAACGTATTAATTGGACTTCGGACCTGCATTTCAAGTTTACAACAGCAATAAGTGCCTTGGGAGATCAGAAAGCTCGTCCTAAAGCGATTATGGACATGATGGGTCTGGAGAATTTAACAATGCGCCAGGTTGCAAGCCATCTTCAGAAATACAAAAATCAAGTACGGCGAATTAACGAGACTGGCATATTTACTGGAAGAAGTAGTTTAAGCAAAGTTTCTGGTGGGAATGAGTTTGTGCCAGCAAAGCAAAGTTCTCTGGTATACCAGAAGAAACCAGGGACTTCAAGGTTTGGAGTAGGAGGTAAACCAACAGAATCAACTACTCCAAATTCTTTGACCAGATCTTCAATCTCTGTAGCTAGTTTCGACAATCATGACTACAGAATGCAGAATCTAAACTCAAGCCATGGAGGCTTGTCTCGCTACGGAAATACCAATAGCCCCAATGATTCTCTAAGAGCTGATGGTTGGTTTCAAAATATGAACCAGATACCAGAAACAAGCAGTTTTGCATACAAAACTGGCATGAAGGAAATATTGAAGAAAAAACTGAAAGTAACTGAAAACTTACCGGGCATGACGTACATGGCTTCAGAGGTTGATCCTCCCAACATGTCAACCAATACATTCCAGCTCCCCGACGAGCCTCCTGCCGTGCATCAAAAGCAAAATTGTACTCCTGCTTTGACTGATCCCTTCAATTCCATAAACAAGGAAACACCAATTGCAGGAACAACAGAAGTGCTTGAGCTACAGAAGATGAACCTACCAAGTATGAACTACTCGACTCCAGAGGTAGATTCTCCTCACATGTCGGCAAACGCATTTCAGTTCTCAAATGAGGTTTCAGTTGCGAATCAAGAGCAAAATTGTACTCTAATTGTAAGTGCTCCCGTGAACTCTGAAATTCGGGACGAGTTTTCAACAGAAGATCAACGAAGTGAATTATTTGAGCCCATGCCAATGAATTTACCAAGTATGACACCAGACAGTGCTTCTGTGATCCAAGAGCAATATTATGCTCCAGTTAATTCCTCCAGTTCCAAAACTCGGAACAGTTTTTCAACAGAAGTCGCAGGAACAAATGAACTATTTGAGCTTGTGCTAAAGGATTCACCTAGTATGGAGTACATGACTAAGGAGGATGCTTCTCCCCACAAATCAGAAAATGCATCTCAGCTCACGAATGAGCCTCTCGGCATGGACCTACAACAATACCGTACTCCTCATGAGAACAAGTTTTCTGCGAGAACAACTGAACTTGAGTTTCTGGCAAAAGAAACACCTTCACGCAATGCTAACAATCTAGGGAACCTTCCAGCAAACTTCAACGATGGAAGCCAGAAGCTGGATACTCTAGCAGGAGAGGCACCTCCTGCAATTTTATCAAATGACCACCAGCACTATACTCCAGTTTTCACCGTTCAATTTGCTTTCCACAATCAGAACAAGGTTTCTCTAGTAGCAACTGAACTTCAGCTTCTGGAGGAGCAAACAGCTTCAAGCAATGCAAAAAAGTTACAGAACCTCCCAACAAAATTCAACAGTGGAAGCCAGAAGCTGGATACTATAACAGGAGAGGCACCTCCTGCAATTTCATTAAATGAGCACCAGAACTATACTCCATCTTTCACCATTCCATTCGATTCCCAAAATCAGAACAAGGTTTCTCCAGTAACAACTGAATTGGGGGAACAAAAAGCTTCAAGCAATGCAAAAGATATAGAGAACCTTCCAGTATACTTCAACAGTGGAAGCCAGGAGCTGAATAATTTAACAGGAGACGCTCCTCCAGCTATTTCATCAAATGAGCACCAGCCACTGTCGGAGTATGCTGACCTGTTGAAAATGCTTGAAGCAGATCCGGAGTACAATTCTTTTGGCAATGCACCATATGCAGGCAATTTTGATGAATACCGTGAATGGCTGGAGAGATCTTTATTTGGAGATAGCACAGAACAACATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000156 GO:0000160 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009735 GO:0009736 GO:0009755 GO:0009873 GO:0009888 GO:0009889 GO:0009987 GO:0010015 GO:0010033 GO:0010073 GO:0010075 GO:0010082 GO:0010119 GO:0010150 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022622 GO:0023052 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032870 GO:0035556 GO:0040008 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048638 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051239 GO:0051252 GO:0051716 GO:0060089 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071368 GO:0071369 GO:0071495 GO:0080050 GO:0080090 GO:0080113 GO:0090693 GO:0099402 GO:0140110 GO:1903506 GO:1905392 GO:2000026 GO:2000112 GO:2000241 GO:2000280 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

Myb_DNA-binding Response_reg

Protein Analysis

920

Amino Acids

102.48

Weight (kDa)

5.06

Isoelectric Point (pI)

47.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Response_reg	PF00072	18 - 125	3.3e-11	Response regulator receiver domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017654)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g26570
malus_domestica	MD13G1034600.v1.1 MD16G1036100.v1.1
prunus_persica	Prupe.1G317900_v2.0.a1
rosa_laevigata	RLG00000006691
rosa_multiflora	Rmu_sc0000459.1_g000011
rosa_roxburghii	Rroxscaffold_5G00375600
rosa_samantha	Rh4AG330200 Rh4BG337800 Rh4CG353200 Rh4DG333800
rosa_wichuraiana	Rw4G028640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	39
Acc36I	ACCTGC	2 cut(s)	488, 825
AccB1I	GGYRCC	2 cut(s)	2164, 2377
AccB7I	CCANNNNNTGG	2 cut(s)	2143, 2356
AccI	GTMKAC	3 cut(s)	390, 1056, 2535
AccIII	TCCGGA	1 cut(s)	2659
AclWI	GGATC	7 cut(s)	186, 372, 761, 1391, 1481, 1792, 2651
AcoI	YGGCCR	1 cut(s)	452
AcsI	RAATTY	6 cut(s)	32, 904, 1123, 1701, 1762, 2333
AcuI	CTGAAG	4 cut(s)	459, 920, 1371, 2255
AfaI	GTAC	8 cut(s)	89, 957, 1379, 1471, 1669, 1925, 2017, 2666
AfiI	CCNNNNNNNGG	7 cut(s)	412, 542, 1523, 1845, 2143, 2356, 2693
AflIII	ACRYGT	4 cut(s)	210, 385, 1407, 1605
AhlI	ACTAGT	1 cut(s)	190
AjnI	CCWGG	5 cut(s)	305, 737, 918, 1069, 2553
Alw21I	GWGCWC	3 cut(s)	1687, 2406, 2610
Alw26I	GTCTC	4 cut(s)	8, 965, 1218, 2571
AlwI	GGATC	7 cut(s)	186, 372, 761, 1391, 1481, 1792, 2651
AlwNI	CAGNNNCTG	5 cut(s)	2143, 2278, 2356, 2560, 2617
Ama87I	CYCGRG	1 cut(s)	251
Aor13HI	TCCGGA	1 cut(s)	2659
AoxI	GGCC	4 cut(s)	43, 452, 533, 565
ApoI	RAATTY	6 cut(s)	32, 904, 1123, 1701, 1762, 2333
AseI	ATTAAT	1 cut(s)	801
AspLEI	GCGC	2 cut(s)	40, 918
AspS9I	GGNCC	2 cut(s)	814, 1999
AsuC2I	CCSGG	1 cut(s)	1368
AsuHPI	GGTGA	3 cut(s)	1902, 2206, 2419
AsuNHI	GCTAGC	1 cut(s)	200
AvaI	CYCGRG	1 cut(s)	251
AvaII	GGWCC	2 cut(s)	814, 1999
BalI	TGGCCA	1 cut(s)	454
BanI	GGYRCC	2 cut(s)	2164, 2377
BanII	GRGCYC	1 cut(s)	1752
BbsI	GAAGAC	2 cut(s)	404, 465
Bbv12I	GWGCWC	3 cut(s)	1687, 2406, 2610
BccI	CCATC	6 cut(s)	512, 886, 939, 1253, 2123, 2428
BceAI	ACGGC	3 cut(s)	58, 974, 1433
BcgI	CGANNNNNNTGC	2 cut(s)	1427, 1461
BciT130I	CCWGG	5 cut(s)	307, 739, 920, 1071, 2555
BciVI	GTATCC	2 cut(s)	2140, 2353
BcnI	CCSGG	1 cut(s)	1368
BcoDI	GTCTC	4 cut(s)	8, 965, 1218, 2571
BcuI	ACTAGT	1 cut(s)	190
BfmI	CTRYAG	5 cut(s)	598, 708, 1149, 1174, 1547
BfuAI	ACCTGC	2 cut(s)	488, 825
BfuI	GTATCC	2 cut(s)	2140, 2353
BglI	GCCNNNNNGGC	1 cut(s)	1206
BglII	AGATCT	2 cut(s)	1136, 2729
Bme1390I	CCNGG	6 cut(s)	307, 739, 920, 1071, 1368, 2555
Bme18I	GGWCC	2 cut(s)	814, 1999
BmeT110I	CYCGRG	1 cut(s)	251
BmgT120I	GGNCC	2 cut(s)	814, 1999
BmiI	GGNNCC	3 cut(s)	2108, 2166, 2379
BmrFI	CCNGG	6 cut(s)	307, 739, 920, 1071, 1368, 2555
BmsI	GCATC	6 cut(s)	104, 152, 653, 1462, 1930, 1976
BmtI	GCTAGC	1 cut(s)	204
BpiI	GAAGAC	2 cut(s)	404, 465
BpmI	CTGGAG	9 cut(s)	920, 1569, 1803, 1816, 2190, 2300, 2451, 2571, 2744
BpuEI	CTTGAG	3 cut(s)	1180, 1562, 2078
BpuMI	CCSGG	1 cut(s)	1368
BsaAI	YACGTR	1 cut(s)	388
BsaJI	CCNNGG	4 cut(s)	510, 849, 1070, 1201
BsaWI	WCCGGW	1 cut(s)	2659
BsaXI	ACNNNNNCTCC	2 cut(s)	2307, 2337
Bsc4I	CCNNNNNNNGG	7 cut(s)	412, 542, 1523, 1845, 2143, 2356, 2693
Bse3DI	GCAATG	5 cut(s)	778, 2095, 2308, 2512, 2686
BseAI	TCCGGA	1 cut(s)	2659
BseBI	CCWGG	5 cut(s)	307, 739, 920, 1071, 2555
BseDI	CCNNGG	4 cut(s)	510, 849, 1070, 1201
BseGI	GGATG	3 cut(s)	523, 657, 1945
BseLI	CCNNNNNNNGG	7 cut(s)	412, 542, 1523, 1845, 2143, 2356, 2693
BseMI	GCAATG	5 cut(s)	778, 2095, 2308, 2512, 2686
BseMII	CTCAG	1 cut(s)	1985
BseRI	GAGGAG	5 cut(s)	271, 1590, 2010, 2297, 2574
BshFI	GGCC	4 cut(s)	45, 454, 535, 567
BshNI	GGYRCC	2 cut(s)	2164, 2377
BsiHKAI	GWGCWC	3 cut(s)	1687, 2406, 2610
BsiHKCI	CYCGRG	1 cut(s)	251
BsiSI	CCGG	2 cut(s)	1367, 2660
BslFI	GGGAC	2 cut(s)	1087, 1721
BslI	CCNNNNNNNGG	7 cut(s)	412, 542, 1523, 1845, 2143, 2356, 2693
BsmAI	GTCTC	4 cut(s)	8, 965, 1218, 2571
BsmBI	CGTCTC	1 cut(s)	2571
BsmFI	GGGAC	2 cut(s)	1087, 1721
BsmI	GAATGC	2 cut(s)	298, 1185
BsnI	GGCC	4 cut(s)	45, 454, 535, 567
BsoBI	CYCGRG	1 cut(s)	251
Bsp1286I	GDGCHC	4 cut(s)	1687, 1752, 2406, 2610
Bsp13I	TCCGGA	1 cut(s)	2659
Bsp1407I	TGTACA	1 cut(s)	87
Bsp19I	CCATGG	1 cut(s)	1201
BspANI	GGCC	4 cut(s)	45, 454, 535, 567
BspCNI	CTCAG	1 cut(s)	1984
BspEI	TCCGGA	1 cut(s)	2659
BspHI	TCATGA	3 cut(s)	67, 1168, 2023
BspLI	GGNNCC	3 cut(s)	2108, 2166, 2379
BspMI	ACCTGC	2 cut(s)	488, 825
BspOI	GCTAGC	1 cut(s)	204
BspPI	GGATC	7 cut(s)	186, 372, 761, 1391, 1481, 1792, 2651
BspT107I	GGYRCC	2 cut(s)	2164, 2377
BsrDI	GCAATG	5 cut(s)	778, 2095, 2308, 2512, 2686
BsrGI	TGTACA	1 cut(s)	87
BssECI	CCNNGG	4 cut(s)	510, 849, 1070, 1201
BssNAI	GTATAC	3 cut(s)	391, 1057, 2536
BssT1I	CCWWGG	3 cut(s)	510, 849, 1201
Bst1107I	GTATAC	3 cut(s)	391, 1057, 2536
Bst2UI	CCWGG	5 cut(s)	307, 739, 920, 1071, 2555
Bst6I	CTCTTC	1 cut(s)	603
BstAPI	GCANNNNNTGC	5 cut(s)	247, 845, 2171, 2384, 2690
BstAUI	TGTACA	1 cut(s)	87
BstBAI	YACGTR	1 cut(s)	388
BstC8I	GCNNGC	8 cut(s)	141, 202, 243, 331, 660, 928, 1035, 2695
BstDEI	CTNAG	4 cut(s)	482, 1250, 1932, 1971
BstDSI	CCRYGG	1 cut(s)	1201
BstENI	CCTNNNNNAGG	1 cut(s)	540
BstF5I	GGATG	3 cut(s)	523, 657, 1945
BstHHI	GCGC	2 cut(s)	40, 918
BstMAI	GTCTC	4 cut(s)	8, 965, 1218, 2571
BstNI	CCWGG	5 cut(s)	307, 739, 920, 1071, 2555
BstNSI	RCATGY	4 cut(s)	214, 245, 1411, 1609
BstSCI	CCNGG	6 cut(s)	305, 737, 918, 1069, 1366, 2553
BstSFI	CTRYAG	5 cut(s)	598, 708, 1149, 1174, 1547
BstV2I	GAAGAC	2 cut(s)	404, 465
BstX2I	RGATCY	5 cut(s)	364, 753, 1136, 2656, 2729
BstXI	CCANNNNNNTGG	1 cut(s)	358
BstYI	RGATCY	5 cut(s)	364, 753, 1136, 2656, 2729
BstZ17I	GTATAC	3 cut(s)	391, 1057, 2536
BsuI	GTATCC	2 cut(s)	2140, 2353
BsuRI	GGCC	4 cut(s)	45, 454, 535, 567
BtgI	CCRYGG	1 cut(s)	1201
BtsCI	GGATG	3 cut(s)	523, 657, 1945
BtsI	GCAGTG	1 cut(s)	245
BtsIMutI	CAGTG	8 cut(s)	105, 245, 483, 561, 1792, 2347, 2551, 2615
BveI	ACCTGC	2 cut(s)	488, 825
Cac8I	GCNNGC	8 cut(s)	141, 202, 243, 331, 660, 928, 1035, 2695
CaiI	CAGNNNCTG	5 cut(s)	2143, 2278, 2356, 2560, 2617
CciI	TCATGA	3 cut(s)	67, 1168, 2023
CfoI	GCGC	2 cut(s)	40, 918
Cfr13I	GGNCC	2 cut(s)	814, 1999
CseI	GACGC	1 cut(s)	2588
Csp6I	GTAC	8 cut(s)	88, 956, 1378, 1470, 1668, 1924, 2016, 2665
CviQI	GTAC	8 cut(s)	88, 956, 1378, 1470, 1668, 1924, 2016, 2665
DdeI	CTNAG	4 cut(s)	482, 1250, 1932, 1971
EaeI	YGGCCR	1 cut(s)	452
Eam1104I	CTCTTC	1 cut(s)	603
EarI	CTCTTC	1 cut(s)	603
Eco130I	CCWWGG	3 cut(s)	510, 849, 1201
Eco147I	AGGCCT	1 cut(s)	535
Eco24I	GRGCYC	1 cut(s)	1752
Eco47I	GGWCC	2 cut(s)	814, 1999
Eco57I	CTGAAG	4 cut(s)	459, 920, 1371, 2255
Eco88I	CYCGRG	1 cut(s)	251
EcoNI	CCTNNNNNAGG	1 cut(s)	540
EcoRII	CCWGG	5 cut(s)	305, 737, 918, 1069, 2553
EcoT14I	CCWWGG	3 cut(s)	510, 849, 1201
EcoT22I	ATGCAT	1 cut(s)	1969
EcoT38I	GRGCYC	1 cut(s)	1752
ErhI	CCWWGG	3 cut(s)	510, 849, 1201
Esp3I	CGTCTC	1 cut(s)	2571
FaqI	GGGAC	2 cut(s)	1087, 1721
FauNDI	CATATG	1 cut(s)	2689
FblI	GTMKAC	3 cut(s)	390, 1056, 2535
FokI	GGATG	3 cut(s)	530, 664, 1952
FriOI	GRGCYC	1 cut(s)	1752
FspI	TGCGCA	1 cut(s)	39
GlaI	GCGC	2 cut(s)	39, 917
GsuI	CTGGAG	9 cut(s)	920, 1569, 1803, 1816, 2190, 2300, 2451, 2571, 2744
HaeIII	GGCC	4 cut(s)	45, 454, 535, 567
HapII	CCGG	2 cut(s)	1367, 2660
HgaI	GACGC	1 cut(s)	2588
HhaI	GCGC	2 cut(s)	40, 918
Hin6I	GCGC	2 cut(s)	38, 916
HinP1I	GCGC	2 cut(s)	38, 916
HincII	GTYRAC	1 cut(s)	1413
HindII	GTYRAC	1 cut(s)	1413
HindIII	AAGCTT	1 cut(s)	2496
HpaII	CCGG	2 cut(s)	1367, 2660
HphI	GGTGA	3 cut(s)	1902, 2206, 2419
Hpy99I	CGWCG	1 cut(s)	1439
HpyAV	CCTTC	8 cut(s)	250, 498, 578, 1318, 1501, 2091, 2120, 2537
HpyCH4IV	ACGT	3 cut(s)	387, 796, 1376
HpyF3I	CTNAG	4 cut(s)	482, 1250, 1932, 1971
HpySE526I	ACGT	3 cut(s)	387, 796, 1376
HspAI	GCGC	2 cut(s)	38, 916
Kpn2I	TCCGGA	1 cut(s)	2659
LmnI	GCTCC	7 cut(s)	326, 1434, 1690, 1822, 2284, 2557, 2587
LweI	GCATC	6 cut(s)	104, 152, 653, 1462, 1930, 1976
MaeII	ACGT	3 cut(s)	387, 796, 1376
MaeIII	GTNAC	4 cut(s)	124, 1351, 2313, 2470
MfeI	CAATTG	1 cut(s)	1518
MflI	RGATCY	5 cut(s)	364, 753, 1136, 2656, 2729
MhlI	GDGCHC	4 cut(s)	1687, 1752, 2406, 2610
MlsI	TGGCCA	1 cut(s)	454
MluNI	TGGCCA	1 cut(s)	454
MlyI	GAGTC	2 cut(s)	479, 1576
MmeI	TCCRAC	1 cut(s)	2601
Mox20I	TGGCCA	1 cut(s)	454
Mph1103I	ATGCAT	1 cut(s)	1969
MroI	TCCGGA	1 cut(s)	2659
MscI	TGGCCA	1 cut(s)	454
MseI	TTAA	9 cut(s)	48, 207, 801, 908, 966, 1001, 1824, 2396, 2570
MslI	CAYNNNNRTG	3 cut(s)	209, 771, 1757
Msp20I	TGGCCA	1 cut(s)	454
MspA1I	CMGCKG	1 cut(s)	662
MspI	CCGG	2 cut(s)	1367, 2660
MspR9I	CCNGG	6 cut(s)	307, 739, 920, 1071, 1368, 2555
MunI	CAATTG	1 cut(s)	1518
Mva1269I	GAATGC	2 cut(s)	298, 1185
MvaI	CCWGG	5 cut(s)	307, 739, 920, 1071, 2555
NciI	CCSGG	1 cut(s)	1368
NcoI	CCATGG	1 cut(s)	1201
NdeI	CATATG	1 cut(s)	2689
NheI	GCTAGC	1 cut(s)	200
NlaIV	GGNNCC	3 cut(s)	2108, 2166, 2379
NmeAIII	GCCGAG	1 cut(s)	1971
NsbI	TGCGCA	1 cut(s)	39
NsiI	ATGCAT	1 cut(s)	1969
NspI	RCATGY	4 cut(s)	214, 245, 1411, 1609
PaeI	GCATGC	1 cut(s)	245
PaeR7I	CTCGAG	1 cut(s)	251
PagI	TCATGA	3 cut(s)	67, 1168, 2023
PceI	AGGCCT	1 cut(s)	535
PciI	ACATGT	3 cut(s)	210, 1407, 1605
PctI	GAATGC	2 cut(s)	298, 1185
PfeI	GAWTC	9 cut(s)	722, 742, 1109, 1186, 1244, 1595, 1651, 1907, 2441
PflMI	CCANNNNNTGG	2 cut(s)	2143, 2356
PleI	GAGTC	2 cut(s)	478, 1576
PpsI	GAGTC	2 cut(s)	478, 1576
Ppu21I	YACGTR	1 cut(s)	388
PscI	ACATGT	3 cut(s)	210, 1407, 1605
PshBI	ATTAAT	1 cut(s)	801
Psp6I	CCWGG	5 cut(s)	305, 737, 918, 1069, 2553
PspGI	CCWGG	5 cut(s)	305, 737, 918, 1069, 2553
PspN4I	GGNNCC	3 cut(s)	2108, 2166, 2379
PspPI	GGNCC	2 cut(s)	814, 1999
PstNI	CAGNNNCTG	5 cut(s)	2143, 2278, 2356, 2560, 2617
PsuI	RGATCY	5 cut(s)	364, 753, 1136, 2656, 2729
PvuII	CAGCTG	1 cut(s)	662
RsaI	GTAC	8 cut(s)	89, 957, 1379, 1471, 1669, 1925, 2017, 2666
RsaNI	GTAC	8 cut(s)	88, 956, 1378, 1470, 1668, 1924, 2016, 2665
RseI	CAYNNNNRTG	3 cut(s)	209, 771, 1757
SaqAI	TTAA	9 cut(s)	48, 207, 801, 908, 966, 1001, 1824, 2396, 2570
Sau96I	GGNCC	2 cut(s)	814, 1999
SchI	GAGTC	2 cut(s)	479, 1576
ScrFI	CCNGG	6 cut(s)	307, 739, 920, 1071, 1368, 2555
SduI	GDGCHC	4 cut(s)	1687, 1752, 2406, 2610
SfaNI	GCATC	6 cut(s)	104, 152, 653, 1462, 1930, 1976
SfcI	CTRYAG	5 cut(s)	598, 708, 1149, 1174, 1547
Sfr274I	CTCGAG	1 cut(s)	251
SinI	GGWCC	2 cut(s)	814, 1999
SlaI	CTCGAG	1 cut(s)	251
SmiMI	CAYNNNNRTG	3 cut(s)	209, 771, 1757
SmlI	CTYRAG	4 cut(s)	251, 1195, 1541, 2057
SmoI	CTYRAG	4 cut(s)	251, 1195, 1541, 2057
SpeI	ACTAGT	1 cut(s)	190
SphI	GCATGC	1 cut(s)	245
SseBI	AGGCCT	1 cut(s)	535
SspI	AATATT	2 cut(s)	1332, 1811
StuI	AGGCCT	1 cut(s)	535
StyD4I	CCNGG	6 cut(s)	305, 737, 918, 1069, 1366, 2553
StyI	CCWWGG	3 cut(s)	510, 849, 1201
TaiI	ACGT	3 cut(s)	390, 799, 1379
TaqI	TCGA	6 cut(s)	63, 135, 252, 1161, 1580, 2439
TatI	WGTACW	5 cut(s)	87, 1469, 1667, 1923, 2664
TfiI	GAWTC	9 cut(s)	722, 742, 1109, 1186, 1244, 1595, 1651, 1907, 2441
Tru1I	TTAA	9 cut(s)	48, 207, 801, 908, 966, 1001, 1824, 2396, 2570
Tru9I	TTAA	9 cut(s)	48, 207, 801, 908, 966, 1001, 1824, 2396, 2570
TscAI	CASTG	8 cut(s)	105, 252, 483, 568, 1792, 2347, 2551, 2622
TspGWI	ACGGA	1 cut(s)	1236
TspRI	CASTG	8 cut(s)	105, 252, 483, 568, 1792, 2347, 2551, 2622
Van91I	CCANNNNNTGG	2 cut(s)	2143, 2356
VpaK11BI	GGWCC	2 cut(s)	814, 1999
VspI	ATTAAT	1 cut(s)	801
XagI	CCTNNNNNAGG	1 cut(s)	540
XapI	RAATTY	6 cut(s)	32, 904, 1123, 1701, 1762, 2333
XbaI	TCTAGA	2 cut(s)	466, 580
XceI	RCATGY	4 cut(s)	214, 245, 1411, 1609
XhoI	CTCGAG	1 cut(s)	251
XmiI	GTMKAC	3 cut(s)	390, 1056, 2535
Zsp2I	ATGCAT	1 cut(s)	1969

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.