Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0017654

Physical Location & Seq

Genomic Coordinates Forward (+)

64066720 .. 64070376

Gene Structure 3657 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG330200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2763 bp

ATGGACAACATGAGTTTTATAGAGACTGACGGTATTCCTGAATTTGCGCACGGCCTTAAAATCCTGCTGTTCGATCATGACACATTTTCCCTCATGTACACAGCATCAGTGCTTGAACAATACTCATTCCATGTTACTGCAATCGAGCAGGCATCTGTCGCTTTGTCCATGATTAGTGAACAAAAGGATCACTATGAACTAGTAATGGCTAGCATTAACATGTCTGATATTGAGAGTGTTTCTTTTCTGCATGCACTGCTCGAGAAGGATATTCCTGTCATATTGATCTCCTCTGAAATGAATGCTGACATGACCAGGAAAGCCCTAGACAAAGGAGCAAGCATTATCCTTGAGAAACCCATTCCTTTGGAGGATCTGAAAAATGTGTGGCAACACGTATACAGGAAGACAAACCCAATGGAGGACATAGGTAAACCAAATTGTGAAGATAAGATAGACTGGCCAAGGGACTATCAAGAGTCTTCAGTGCTTAGACCTTCCTTTGCAGGTATTGAAACCAAGGAGGATGGTGCACTGTGTAGGCCTGATACAGGGCATTATTGGAATGGCACTGGCCTTGAAACTGATCTAGAAGGTTTGCATAGCTATAGCATCGAAGAGTTGTTCCCAACAGAGAGCCAAAAACAAAAAGCATCTGGGATGAAGCCAGCTGATGATACCGACGAACAAGGAAAACAAAAAAAATTGAAACTAACTGTAGAGCAAACTGATTCTGACAGTATAGCCAGGAATCAGGAAGGGATCTCCAAGAAACATAGCAATGACAAAACAGATAAGAAAGAACGTATTAATTGGACTTCTGACCTGCATTTCAAGTTTACAACAGCAATAAGTGCCTTGGGAGATCAGAAAGCTCGTCCTAAATCGATTATGAACATGATGGGTGTGGAGAATTTAACAATGCGCCAGGTTGCAAGCCATCTTCAGAAATACAAAAATCAAGTACGGCGAATTAACGAGACTGGCATATTTACTGGAAGAAGTAGTTTAAGCAAAGTTTCTGGTGGGAATGAGTTTGTGCCAGCAAAGCAAAGTTCTCTGGTATACCAGAAGAAACCAGGGACTTCAAGGTTTGGAGTAGGAGGTAAACCAACAGAATCAACTACTCCAAATTCTTTGACCAGATCTTCGATCTCTGTAGCTAGTTTCGACAATCATGACTACAGAATGCAGAATCTAAACTCAAGCCATGGAGGCTTATCTCGCTACGGAAATACCAATAGCCCCAATGATTCTCTAAGAACTGATGGTTGGTTTCAAAATATGAACCAGATACCAGAAACAAGCAGTTTTGCATACAAAACTGGCATGAAGGAAATATTGAAGAAAAAACTGAAAGTAACTGAAAACTTACCGGGAATGACTTCAGAGGTTGATCCTCCCAACGTATTGGTTGACATATTCCAGCTCCCCGACGAGCCTCCTGCCGTGCATCAAAAGCAAAATTGTACTCCTGCTTTGACTGATCCCTTCAATTCCATAAACAAGGAAACACCAATTGCAGGAACAACAGAAGTGCTTGAACTACAGAAGATGAACCAACCAAGTATGAATTACTCGACTCCAGAGGCAGATTCTCCTTACATGTTGGCAAATGCATTTCAGTTCTCAAATGAGGTTTCAGTTGCGAATCAAGAACAAAATTGTACTCTAATTGTAAGTGCTCCCATGAACTCTGAAATTCGGGACGAGTTTTCAACAGAAGATCAACGAAGTGAATTATTTGAGCCCATGCCAATGAATTTACCAAGCATGACACCAGACAGTGCTTCTGTGAACCAAGAGCAATATTATGCTCCAGTTAATTCCTCCAGTTCCGAAACTCGGAACAGTTTTTCAACAGAAGTTGCAGGAACAAATAAACTACTTGAGCTTGTGCTAAAGGATTCACCTAGTATGGAGTGCATGACTAAGGAAGATGCTTCTCCCCACAAATCAGAAAATGCATCTCAGCTCCCAAATGAGCCTCTCGGCATGGACCTACAACAATACCGTACTCCTCATGAGAACAAGTTTGCTGCGAGAACAACTGAACTTGAGTTTCTGGCAAAAGAAACACCTTCAAGCAATGCTAACAATCTAGGGAACCTTCCAGCAAACTTCAACGATGGAAGCCAGAAGCTGGATACTCTAGCAGGAGAGGCACTTCCTGCGATTTTATCAAATGAACACCAGCACTATACTCCAGTTTTTACTGTTCCATTTGCTTTCCAAAATCAGAACAAGGTTTCTCTAGTAGCAACTGAACTTCAGCTTCTGGAGGAGCAAACAGCTTCAAGCAATCCAAAAAACTTACAGAACCTCCCAACAAAATTCAACAGTGGAAGCCAGAAGCTGGATACTATAACAGGAGAGGCACCTCCTGTGATTTCATTAAATGAGCACCAGCACTATACTCCATCTTTCACCATTCCATTCGATTCCCAAAATCAGAACAAGGTTTCTCCAGTAACAACTGAATTGGGGGAACAAAAAGCTTCAAGCAATGCAAAAGATATAGAGAACCTTCCAGTATACTTCAACAGTGGAAGCCAGGAGCTGAATAATTTAACAGGAGACGTTCCTCCAGCTATTTCATCAAATGAGCACCAGCCACTGTCGGAGTATGCTGACCTGTTGAAAATGCTTGAAGCAGATCCGGAGTACAATTCTTTTGGCAATGCACCATTTGCAGGCAATTTTGATGAATACCGTGAATGGCTGGAGAGATCTTTATTTGGAGATAGCACAGAACAACATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000156 GO:0000160 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009735 GO:0009736 GO:0009755 GO:0009873 GO:0009888 GO:0009889 GO:0009987 GO:0010015 GO:0010033 GO:0010073 GO:0010075 GO:0010082 GO:0010119 GO:0010150 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022622 GO:0023052 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032870 GO:0035556 GO:0040008 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048638 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051239 GO:0051252 GO:0051716 GO:0060089 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071368 GO:0071369 GO:0071495 GO:0080050 GO:0080090 GO:0080113 GO:0090693 GO:0099402 GO:0140110 GO:1903506 GO:1905392 GO:2000026 GO:2000112 GO:2000241 GO:2000280 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

Myb_DNA-binding Response_reg

Protein Analysis

920

Amino Acids

102.49

Weight (kDa)

4.91

Isoelectric Point (pI)

47.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Response_reg	PF00072	21 - 128	1.2e-11	Response regulator receiver domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0017654)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g26570
malus_domestica	MD13G1034600.v1.1 MD16G1036100.v1.1
prunus_persica	Prupe.1G317900_v2.0.a1
rosa_laevigata	RLG00000006691
rosa_multiflora	Rmu_sc0000459.1_g000011
rosa_roxburghii	Rroxscaffold_5G00375600
rosa_samantha	Rh4AG330200 Rh4BG337800 Rh4CG353200 Rh4DG333800
rosa_wichuraiana	Rw4G028640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	48
Acc36I	ACCTGC	2 cut(s)	497, 834
AccB1I	GGYRCC	1 cut(s)	2377
AccB7I	CCANNNNNTGG	2 cut(s)	2143, 2356
AccI	GTMKAC	3 cut(s)	399, 1065, 2535
AccIII	TCCGGA	1 cut(s)	2659
AclWI	GGATC	6 cut(s)	195, 381, 770, 1391, 1481, 2651
AcoI	YGGCCR	1 cut(s)	461
AcsI	RAATTY	6 cut(s)	41, 913, 1132, 1701, 1762, 2333
AcuI	CTGAAG	4 cut(s)	468, 929, 1371, 2255
AfaI	GTAC	6 cut(s)	98, 966, 1471, 1669, 2017, 2666
AfiI	CCNNNNNNNGG	7 cut(s)	421, 551, 1523, 1845, 2143, 2356, 2693
AflIII	ACRYGT	3 cut(s)	219, 394, 1605
AhlI	ACTAGT	1 cut(s)	199
AjnI	CCWGG	5 cut(s)	314, 746, 927, 1078, 2553
AjuI	GAANNNNNNNTTGG	2 cut(s)	1255, 1287
Alw21I	GWGCWC	4 cut(s)	535, 1687, 2406, 2610
Alw26I	GTCTC	3 cut(s)	17, 974, 2571
Alw44I	GTGCAC	1 cut(s)	531
AlwI	GGATC	6 cut(s)	195, 381, 770, 1391, 1481, 2651
AlwNI	CAGNNNCTG	5 cut(s)	2143, 2278, 2356, 2560, 2617
Ama87I	CYCGRG	1 cut(s)	260
Aor13HI	TCCGGA	1 cut(s)	2659
AoxI	GGCC	4 cut(s)	52, 461, 542, 574
ApaLI	GTGCAC	1 cut(s)	531
ApeKI	GCWGC	1 cut(s)	2039
ApoI	RAATTY	6 cut(s)	41, 913, 1132, 1701, 1762, 2333
ArsI	GACNNNNNNTTYG	2 cut(s)	1132, 1164
AseI	ATTAAT	1 cut(s)	810
Asp700I	GAANNNNTTC	1 cut(s)	1384
AspLEI	GCGC	2 cut(s)	49, 927
AspS9I	GGNCC	1 cut(s)	1999
AsuC2I	CCSGG	1 cut(s)	1377
AsuHPI	GGTGA	2 cut(s)	1902, 2419
AsuNHI	GCTAGC	1 cut(s)	209
AvaI	CYCGRG	1 cut(s)	260
AvaII	GGWCC	1 cut(s)	1999
BaeGI	GKGCMC	1 cut(s)	535
BalI	TGGCCA	1 cut(s)	463
BanI	GGYRCC	1 cut(s)	2377
BanII	GRGCYC	1 cut(s)	1752
BbsI	GAAGAC	2 cut(s)	413, 474
Bbv12I	GWGCWC	4 cut(s)	535, 1687, 2406, 2610
BbvI	GCAGC	1 cut(s)	2026
BccI	CCATC	6 cut(s)	521, 895, 948, 1262, 2123, 2428
BceAI	ACGGC	3 cut(s)	67, 983, 1433
BcgI	CGANNNNNNTGC	2 cut(s)	1427, 1461
BciT130I	CCWGG	5 cut(s)	316, 748, 929, 1080, 2555
BciVI	GTATCC	2 cut(s)	2140, 2353
BcnI	CCSGG	1 cut(s)	1377
BcoDI	GTCTC	3 cut(s)	17, 974, 2571
BcuI	ACTAGT	1 cut(s)	199
BfaI	CTAG	9 cut(s)	200, 210, 326, 590, 1164, 1914, 2102, 2153, 2255
BfmI	CTRYAG	5 cut(s)	607, 717, 1158, 1183, 1547
BfuAI	ACCTGC	2 cut(s)	497, 834
BfuI	GTATCC	2 cut(s)	2140, 2353
BglI	GCCNNNNNGGC	1 cut(s)	1215
BglII	AGATCT	2 cut(s)	1145, 2729
BisI	GCNGC	1 cut(s)	2040
BlsI	GCNGC	1 cut(s)	2041
Bme1390I	CCNGG	6 cut(s)	316, 748, 929, 1080, 1377, 2555
Bme18I	GGWCC	1 cut(s)	1999
BmeT110I	CYCGRG	1 cut(s)	260
BmgT120I	GGNCC	1 cut(s)	1999
BmiI	GGNNCC	2 cut(s)	2108, 2379
BmrFI	CCNGG	6 cut(s)	316, 748, 929, 1080, 1377, 2555
BmsI	GCATC	7 cut(s)	113, 161, 621, 662, 1462, 1930, 1976
BmtI	GCTAGC	1 cut(s)	213
BpiI	GAAGAC	2 cut(s)	413, 474
BpmI	CTGGAG	8 cut(s)	1569, 1803, 1816, 2190, 2300, 2451, 2571, 2744
BpuEI	CTTGAG	4 cut(s)	371, 1189, 1910, 2078
BpuMI	CCSGG	1 cut(s)	1377
Bsa29I	ATCGAT	1 cut(s)	887
BsaAI	YACGTR	1 cut(s)	397
BsaJI	CCNNGG	5 cut(s)	464, 519, 858, 1079, 1210
BsaWI	WCCGGW	1 cut(s)	2659
BsaXI	ACNNNNNCTCC	2 cut(s)	2307, 2337
Bsc4I	CCNNNNNNNGG	7 cut(s)	421, 551, 1523, 1845, 2143, 2356, 2693
Bse3DI	GCAATG	4 cut(s)	787, 2095, 2512, 2686
BseAI	TCCGGA	1 cut(s)	2659
BseBI	CCWGG	5 cut(s)	316, 748, 929, 1080, 2555
BseCI	ATCGAT	1 cut(s)	887
BseDI	CCNNGG	5 cut(s)	464, 519, 858, 1079, 1210
BseGI	GGATG	2 cut(s)	532, 666
BseLI	CCNNNNNNNGG	7 cut(s)	421, 551, 1523, 1845, 2143, 2356, 2693
BseMI	GCAATG	4 cut(s)	787, 2095, 2512, 2686
BseMII	CTCAG	1 cut(s)	1985
BseRI	GAGGAG	3 cut(s)	280, 2010, 2297
BseSI	GKGCMC	1 cut(s)	535
BseXI	GCAGC	1 cut(s)	2026
BshFI	GGCC	4 cut(s)	54, 463, 544, 576
BshNI	GGYRCC	1 cut(s)	2377
BshVI	ATCGAT	1 cut(s)	887
BsiHKAI	GWGCWC	4 cut(s)	535, 1687, 2406, 2610
BsiHKCI	CYCGRG	1 cut(s)	260
BsiSI	CCGG	2 cut(s)	1376, 2660
BslFI	GGGAC	3 cut(s)	482, 1096, 1721
BslI	CCNNNNNNNGG	7 cut(s)	421, 551, 1523, 1845, 2143, 2356, 2693
BsmAI	GTCTC	3 cut(s)	17, 974, 2571
BsmBI	CGTCTC	1 cut(s)	2571
BsmFI	GGGAC	3 cut(s)	482, 1096, 1721
BsmI	GAATGC	2 cut(s)	307, 1194
BsnI	GGCC	4 cut(s)	54, 463, 544, 576
BsoBI	CYCGRG	1 cut(s)	260
Bsp1286I	GDGCHC	5 cut(s)	535, 1687, 1752, 2406, 2610
Bsp13I	TCCGGA	1 cut(s)	2659
Bsp1407I	TGTACA	1 cut(s)	96
Bsp19I	CCATGG	1 cut(s)	1210
BspANI	GGCC	4 cut(s)	54, 463, 544, 576
BspCNI	CTCAG	1 cut(s)	1984
BspDI	ATCGAT	1 cut(s)	887
BspEI	TCCGGA	1 cut(s)	2659
BspHI	TCATGA	3 cut(s)	76, 1177, 2023
BspLI	GGNNCC	2 cut(s)	2108, 2379
BspMI	ACCTGC	2 cut(s)	497, 834
BspOI	GCTAGC	1 cut(s)	213
BspPI	GGATC	6 cut(s)	195, 381, 770, 1391, 1481, 2651
BspT107I	GGYRCC	1 cut(s)	2377
BsrDI	GCAATG	4 cut(s)	787, 2095, 2512, 2686
BsrGI	TGTACA	1 cut(s)	96
BssECI	CCNNGG	5 cut(s)	464, 519, 858, 1079, 1210
BssNAI	GTATAC	3 cut(s)	400, 1066, 2536
BssT1I	CCWWGG	4 cut(s)	464, 519, 858, 1210
Bst1107I	GTATAC	3 cut(s)	400, 1066, 2536
Bst2UI	CCWGG	5 cut(s)	316, 748, 929, 1080, 2555
Bst6I	CTCTTC	1 cut(s)	612
BstAPI	GCANNNNNTGC	4 cut(s)	256, 854, 2171, 2690
BstAUI	TGTACA	1 cut(s)	96
BstBAI	YACGTR	1 cut(s)	397
BstC8I	GCNNGC	8 cut(s)	150, 211, 252, 340, 669, 937, 1044, 2695
BstDEI	CTNAG	4 cut(s)	491, 1259, 1932, 1971
BstDSI	CCRYGG	1 cut(s)	1210
BstENI	CCTNNNNNAGG	1 cut(s)	549
BstF5I	GGATG	2 cut(s)	532, 666
BstHHI	GCGC	2 cut(s)	49, 927
BstMAI	GTCTC	3 cut(s)	17, 974, 2571
BstNI	CCWGG	5 cut(s)	316, 748, 929, 1080, 2555
BstNSI	RCATGY	3 cut(s)	223, 254, 1609
BstSCI	CCNGG	6 cut(s)	314, 746, 927, 1078, 1375, 2553
BstSFI	CTRYAG	5 cut(s)	607, 717, 1158, 1183, 1547
BstSLI	GKGCMC	1 cut(s)	535
BstV1I	GCAGC	1 cut(s)	2026
BstV2I	GAAGAC	2 cut(s)	413, 474
BstX2I	RGATCY	5 cut(s)	373, 762, 1145, 2656, 2729
BstXI	CCANNNNNNTGG	2 cut(s)	367, 1411
BstYI	RGATCY	5 cut(s)	373, 762, 1145, 2656, 2729
BstZ17I	GTATAC	3 cut(s)	400, 1066, 2536
Bsu15I	ATCGAT	1 cut(s)	887
BsuI	GTATCC	2 cut(s)	2140, 2353
BsuRI	GGCC	4 cut(s)	54, 463, 544, 576
BsuTUI	ATCGAT	1 cut(s)	887
BtgI	CCRYGG	1 cut(s)	1210
BtsCI	GGATG	2 cut(s)	532, 666
BtsI	GCAGTG	1 cut(s)	254
BtsIMutI	CAGTG	9 cut(s)	114, 254, 492, 533, 570, 1792, 2347, 2551, 2615
BveI	ACCTGC	2 cut(s)	497, 834
Cac8I	GCNNGC	8 cut(s)	150, 211, 252, 340, 669, 937, 1044, 2695
CaiI	CAGNNNCTG	5 cut(s)	2143, 2278, 2356, 2560, 2617
CciI	TCATGA	3 cut(s)	76, 1177, 2023
CfoI	GCGC	2 cut(s)	49, 927
Cfr13I	GGNCC	1 cut(s)	1999
ClaI	ATCGAT	1 cut(s)	887
Csp6I	GTAC	6 cut(s)	97, 965, 1470, 1668, 2016, 2665
CviQI	GTAC	6 cut(s)	97, 965, 1470, 1668, 2016, 2665
DdeI	CTNAG	4 cut(s)	491, 1259, 1932, 1971
EaeI	YGGCCR	1 cut(s)	461
Eam1104I	CTCTTC	1 cut(s)	612
EarI	CTCTTC	1 cut(s)	612
Eco130I	CCWWGG	4 cut(s)	464, 519, 858, 1210
Eco147I	AGGCCT	1 cut(s)	544
Eco24I	GRGCYC	1 cut(s)	1752
Eco47I	GGWCC	1 cut(s)	1999
Eco57I	CTGAAG	4 cut(s)	468, 929, 1371, 2255
Eco88I	CYCGRG	1 cut(s)	260
EcoNI	CCTNNNNNAGG	1 cut(s)	549
EcoRII	CCWGG	5 cut(s)	314, 746, 927, 1078, 2553
EcoT14I	CCWWGG	4 cut(s)	464, 519, 858, 1210
EcoT22I	ATGCAT	2 cut(s)	1621, 1969
EcoT38I	GRGCYC	1 cut(s)	1752
ErhI	CCWWGG	4 cut(s)	464, 519, 858, 1210
Esp3I	CGTCTC	1 cut(s)	2571
FaqI	GGGAC	3 cut(s)	482, 1096, 1721
FblI	GTMKAC	3 cut(s)	399, 1065, 2535
Fnu4HI	GCNGC	1 cut(s)	2040
FokI	GGATG	2 cut(s)	539, 673
FriOI	GRGCYC	1 cut(s)	1752
Fsp4HI	GCNGC	1 cut(s)	2040
FspBI	CTAG	9 cut(s)	200, 210, 326, 590, 1164, 1914, 2102, 2153, 2255
FspI	TGCGCA	1 cut(s)	48
GlaI	GCGC	2 cut(s)	48, 926
GluI	GCNGC	1 cut(s)	2040
GsuI	CTGGAG	8 cut(s)	1569, 1803, 1816, 2190, 2300, 2451, 2571, 2744
HaeIII	GGCC	4 cut(s)	54, 463, 544, 576
HapII	CCGG	2 cut(s)	1376, 2660
HhaI	GCGC	2 cut(s)	49, 927
Hin6I	GCGC	2 cut(s)	47, 925
HinP1I	GCGC	2 cut(s)	47, 925
HincII	GTYRAC	1 cut(s)	1417
HindII	GTYRAC	1 cut(s)	1417
HindIII	AAGCTT	1 cut(s)	2496
HpaII	CCGG	2 cut(s)	1376, 2660
HphI	GGTGA	2 cut(s)	1902, 2419
Hpy99I	CGWCG	2 cut(s)	686, 1439
HpyAV	CCTTC	9 cut(s)	259, 507, 587, 752, 1327, 1501, 2091, 2120, 2537
HpyCH4IV	ACGT	4 cut(s)	396, 805, 1407, 2580
HpyF3I	CTNAG	4 cut(s)	491, 1259, 1932, 1971
HpySE526I	ACGT	4 cut(s)	396, 805, 1407, 2580
HspAI	GCGC	2 cut(s)	47, 925
Kpn2I	TCCGGA	1 cut(s)	2659
LmnI	GCTCC	7 cut(s)	335, 1434, 1690, 1822, 1980, 2284, 2557
Lsp1109I	GCAGC	1 cut(s)	2026
LweI	GCATC	7 cut(s)	113, 161, 621, 662, 1462, 1930, 1976
MaeI	CTAG	9 cut(s)	200, 210, 326, 590, 1164, 1914, 2102, 2153, 2255
MaeII	ACGT	4 cut(s)	396, 805, 1407, 2580
MaeIII	GTNAC	3 cut(s)	133, 1360, 2470
MfeI	CAATTG	1 cut(s)	1518
MflI	RGATCY	5 cut(s)	373, 762, 1145, 2656, 2729
MhlI	GDGCHC	5 cut(s)	535, 1687, 1752, 2406, 2610
MlsI	TGGCCA	1 cut(s)	463
MluNI	TGGCCA	1 cut(s)	463
MlyI	GAGTC	2 cut(s)	488, 1576
MmeI	TCCRAC	1 cut(s)	2601
Mox20I	TGGCCA	1 cut(s)	463
Mph1103I	ATGCAT	2 cut(s)	1621, 1969
MroI	TCCGGA	1 cut(s)	2659
MroXI	GAANNNNTTC	1 cut(s)	1384
MscI	TGGCCA	1 cut(s)	463
MseI	TTAA	9 cut(s)	57, 216, 810, 917, 975, 1010, 1824, 2396, 2570
MslI	CAYNNNNRTG	3 cut(s)	218, 780, 1757
Msp20I	TGGCCA	1 cut(s)	463
MspA1I	CMGCKG	1 cut(s)	671
MspI	CCGG	2 cut(s)	1376, 2660
MspR9I	CCNGG	6 cut(s)	316, 748, 929, 1080, 1377, 2555
MunI	CAATTG	1 cut(s)	1518
Mva1269I	GAATGC	2 cut(s)	307, 1194
MvaI	CCWGG	5 cut(s)	316, 748, 929, 1080, 2555
NciI	CCSGG	1 cut(s)	1377
NcoI	CCATGG	1 cut(s)	1210
NheI	GCTAGC	1 cut(s)	209
NlaIV	GGNNCC	2 cut(s)	2108, 2379
NmeAIII	GCCGAG	1 cut(s)	1971
NsbI	TGCGCA	1 cut(s)	48
NsiI	ATGCAT	2 cut(s)	1621, 1969
NspI	RCATGY	3 cut(s)	223, 254, 1609
PaeI	GCATGC	1 cut(s)	254
PaeR7I	CTCGAG	1 cut(s)	260
PagI	TCATGA	3 cut(s)	76, 1177, 2023
PceI	AGGCCT	1 cut(s)	544
PciI	ACATGT	2 cut(s)	219, 1605
PctI	GAATGC	2 cut(s)	307, 1194
PdmI	GAANNNNTTC	1 cut(s)	1384
PfeI	GAWTC	9 cut(s)	731, 751, 1118, 1195, 1253, 1595, 1651, 1907, 2441
PflMI	CCANNNNNTGG	2 cut(s)	2143, 2356
PkrI	GCNGC	1 cut(s)	2041
PleI	GAGTC	2 cut(s)	487, 1576
PpsI	GAGTC	2 cut(s)	487, 1576
Ppu21I	YACGTR	1 cut(s)	397
PscI	ACATGT	2 cut(s)	219, 1605
PshBI	ATTAAT	1 cut(s)	810
Psp6I	CCWGG	5 cut(s)	314, 746, 927, 1078, 2553
PspGI	CCWGG	5 cut(s)	314, 746, 927, 1078, 2553
PspN4I	GGNNCC	2 cut(s)	2108, 2379
PspPI	GGNCC	1 cut(s)	1999
PstNI	CAGNNNCTG	5 cut(s)	2143, 2278, 2356, 2560, 2617
PsuI	RGATCY	5 cut(s)	373, 762, 1145, 2656, 2729
PvuII	CAGCTG	1 cut(s)	671
RsaI	GTAC	6 cut(s)	98, 966, 1471, 1669, 2017, 2666
RsaNI	GTAC	6 cut(s)	97, 965, 1470, 1668, 2016, 2665
RseI	CAYNNNNRTG	3 cut(s)	218, 780, 1757
SaqAI	TTAA	9 cut(s)	57, 216, 810, 917, 975, 1010, 1824, 2396, 2570
SatI	GCNGC	1 cut(s)	2040
Sau96I	GGNCC	1 cut(s)	1999
SchI	GAGTC	2 cut(s)	488, 1576
ScrFI	CCNGG	6 cut(s)	316, 748, 929, 1080, 1377, 2555
SduI	GDGCHC	5 cut(s)	535, 1687, 1752, 2406, 2610
SfaNI	GCATC	7 cut(s)	113, 161, 621, 662, 1462, 1930, 1976
SfcI	CTRYAG	5 cut(s)	607, 717, 1158, 1183, 1547
Sfr274I	CTCGAG	1 cut(s)	260
SinI	GGWCC	1 cut(s)	1999
SlaI	CTCGAG	1 cut(s)	260
SmiMI	CAYNNNNRTG	3 cut(s)	218, 780, 1757
SmlI	CTYRAG	5 cut(s)	260, 350, 1204, 1889, 2057
SmoI	CTYRAG	5 cut(s)	260, 350, 1204, 1889, 2057
SpeI	ACTAGT	1 cut(s)	199
SphI	GCATGC	1 cut(s)	254
SseBI	AGGCCT	1 cut(s)	544
SspI	AATATT	2 cut(s)	1341, 1811
SspMI	CTAG	9 cut(s)	200, 210, 326, 590, 1164, 1914, 2102, 2153, 2255
StuI	AGGCCT	1 cut(s)	544
StyD4I	CCNGG	6 cut(s)	314, 746, 927, 1078, 1375, 2553
StyI	CCWWGG	4 cut(s)	464, 519, 858, 1210
TaiI	ACGT	4 cut(s)	399, 808, 1410, 2583
TaqI	TCGA	9 cut(s)	72, 144, 261, 615, 887, 1151, 1170, 1580, 2439
TatI	WGTACW	4 cut(s)	96, 1469, 1667, 2664
TfiI	GAWTC	9 cut(s)	731, 751, 1118, 1195, 1253, 1595, 1651, 1907, 2441
Tru1I	TTAA	9 cut(s)	57, 216, 810, 917, 975, 1010, 1824, 2396, 2570
Tru9I	TTAA	9 cut(s)	57, 216, 810, 917, 975, 1010, 1824, 2396, 2570
TscAI	CASTG	9 cut(s)	114, 261, 492, 540, 577, 1792, 2347, 2551, 2622
TseI	GCWGC	1 cut(s)	2039
TspGWI	ACGGA	1 cut(s)	1245
TspRI	CASTG	9 cut(s)	114, 261, 492, 540, 577, 1792, 2347, 2551, 2622
Van91I	CCANNNNNTGG	2 cut(s)	2143, 2356
VneI	GTGCAC	1 cut(s)	531
VpaK11BI	GGWCC	1 cut(s)	1999
VspI	ATTAAT	1 cut(s)	810
XagI	CCTNNNNNAGG	1 cut(s)	549
XapI	RAATTY	6 cut(s)	41, 913, 1132, 1701, 1762, 2333
XbaI	TCTAGA	1 cut(s)	589
XceI	RCATGY	3 cut(s)	223, 254, 1609
XhoI	CTCGAG	1 cut(s)	260
XmiI	GTMKAC	3 cut(s)	399, 1065, 2535
XmnI	GAANNNNTTC	1 cut(s)	1384
XspI	CTAG	9 cut(s)	200, 210, 326, 590, 1164, 1914, 2102, 2153, 2255
Zsp2I	ATGCAT	2 cut(s)	1621, 1969

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.