Rh2BG212800

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0017965
Physical Location & Seq
Reverse (-)
20193094 .. 20194254
1161 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG212800.1

Sequence Viewer

Length: 1161 bp
ATGAGGGAGTTGATTCGAAAGCAATACAAGAGCTGGACATCGAAGGAGGAAGAAGCCCTAGCAGCCGGGGTGCATAAGTACGGGTGCGGCAACTGGAAAACCATCCTCAAATATTCCAAATTTGCTCGTTACCTCGCTCGGAAGTCATGCGGTGACCTGTGGAAGAAATGGAGGACAATGGCATTTAGTGTTTCCAATGAGAAACTAAAGACCATGTCTCCCAAGGTAAAGAAGGTTGTCGCTGCCCGCCTGGAGGCCATTAATGATGGGAAAAGCTCTTCTTTTTCTGAAAGAAAAGAGAGGGGTATAACGGGAAGCGGTTGGACGGTGGAGGAGGAAGAAGCCCTAGCCGCCGGGGTGGACAAGTTCGGGACGAGCAAGTGGAAGCACATTCTCGAAGATCCCGAATTCGGCCCTGTCCTCGCTATCCACCCGTGTACATCTCTGGCGAATAAGTGGAGGAACTTGACTGATAGTGTTTCCGAGGAAAAGCTCAGGAGGATGTCTTGTAAGGAAAGGAAAGTTATGGATGCTTCCTTGCGCAGCAAGATTAATGATGATCAGGGGGAGGAGACCGAAAGGGTTTCAAAGATGGAAGAAGACACGGAAGAGAGCACTATGTCAACAAAGGTAAGGAAGGTTGTGGATGATGAGGTTCAGAACTCTGCTAATCCTAATGAGGCAGTGAAGGAGACGGAAAGGGGTCCGGATCATGAGATGGTAGAAGAAGATGAGACCCTAATGATGGATGTTGAAAGTTCTGTTGATCCTGCTGAAGCAGTTAAGGAGAATGAAAGGATTTTGAAAATGAAAGAAGATGCCAAGTCTATGCTACTGTTAGCGAAAGAGATTTGGGGACATTGCTCGACGGAAGACACCAATCAAGTCTCTGCTCCTGCTTGTCCACAAGCAGTGAAGGAGAGCAAAAGGATTTCGGAGATTGCAGAAGACATTGATAGGTTTTCTGCTGCCCCTCCTCCACAGGCGGAGAAAAAGACCAAAATATTTTCAGAAATGGAAGAAGACACTGATCGAGGCCCTGCTCCTGCTCCTGCTCCTCCAAAAGCAGTTGAAGAGACCAAAAGGATTTCGGAGACAGCAGAAGATACCAAATCCATGCTACTGTTCATGAATCAGATTTATGAAAGATTCACGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

386

Amino Acids

43.74

Weight (kDa)

5.47

Isoelectric Point (pI)

51.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 11 - 57 2.6e-06 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017965)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17810
rosa_laevigata RLG00000017651
rosa_multiflora Rmu_sc0007406.1_g000007 Rmu_sc0013358.1_g000003
rosa_roxburghii Rroxscaffold_2G00135440
rosa_rugosa Rorug02G0151700
rosa_samantha Rh2AG200100 Rh2BG212800 Rh2CG204800 Rh2DG207700
rosa_wichuraiana Rw2G015930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 542
AccIII TCCGGA 1 cut(s) 706
AciI CCGC 6 cut(s) 87, 150, 247, 318, 351, 986
AclWI GGATC 3 cut(s) 395, 717, 761
AcsI RAATTY 2 cut(s) 119, 407
AcuI CTGAAG 1 cut(s) 795
AfaI GTAC 2 cut(s) 80, 439
AfiI CCNNNNNNNGG 3 cut(s) 253, 410, 745
AgsI TTSAA 4 cut(s) 588, 755, 805, 1073
AjnI CCWGG 1 cut(s) 249
AluBI AGCT 3 cut(s) 33, 276, 493
AluI AGCT 3 cut(s) 33, 276, 493
Alw21I GWGCWC 1 cut(s) 617
Alw26I GTCTC 7 cut(s) 222, 566, 686, 728, 892, 1070, 1088
AlwI GGATC 3 cut(s) 395, 717, 761
Aor13HI TCCGGA 1 cut(s) 706
AoxI GGCC 3 cut(s) 255, 412, 1036
ApeKI GCWGC 4 cut(s) 62, 242, 543, 968
ApoI RAATTY 2 cut(s) 119, 407
AseI ATTAAT 2 cut(s) 261, 552
AspLEI GCGC 1 cut(s) 543
AspS9I GGNCC 3 cut(s) 413, 704, 1037
AsuC2I CCSGG 2 cut(s) 67, 355
AsuHPI GGTGA 1 cut(s) 164
AsuII TTCGAA 1 cut(s) 16
AvaII GGWCC 1 cut(s) 704
BbsI GAAGAC 4 cut(s) 606, 879, 954, 1029
Bbv12I GWGCWC 1 cut(s) 617
BbvI GCAGC 4 cut(s) 74, 229, 555, 955
BccI CCATC 5 cut(s) 110, 260, 586, 712, 739
BciT130I CCWGG 1 cut(s) 251
BclI TGATCA 1 cut(s) 559
BcnI CCSGG 2 cut(s) 67, 355
BcoDI GTCTC 7 cut(s) 222, 566, 686, 728, 892, 1070, 1088
BfaI CTAG 2 cut(s) 59, 347
BisI GCNGC 6 cut(s) 63, 88, 243, 351, 544, 969
BlsI GCNGC 6 cut(s) 64, 89, 244, 352, 545, 970
Bme1390I CCNGG 3 cut(s) 67, 251, 355
Bme18I GGWCC 1 cut(s) 704
BmgT120I GGNCC 3 cut(s) 413, 704, 1037
BmiI GGNNCC 1 cut(s) 705
BmrFI CCNGG 3 cut(s) 67, 251, 355
BmsI GCATC 2 cut(s) 520, 808
BpiI GAAGAC 4 cut(s) 606, 879, 954, 1029
BpmI CTGGAG 1 cut(s) 272
Bpu10I CCTNAGC 1 cut(s) 494
Bpu14I TTCGAA 1 cut(s) 16
BpuMI CCSGG 2 cut(s) 67, 355
BsaI GGTCTC 3 cut(s) 566, 728, 1070
BsaJI CCNNGG 4 cut(s) 66, 222, 354, 483
BsaWI WCCGGW 1 cut(s) 706
BsaXI ACNNNNNCTCC 2 cut(s) 202, 232
Bsc4I CCNNNNNNNGG 3 cut(s) 253, 410, 745
Bse1I ACTGG 1 cut(s) 98
Bse3DI GCAATG 1 cut(s) 859
BseAI TCCGGA 1 cut(s) 706
BseBI CCWGG 1 cut(s) 251
BseDI CCNNGG 4 cut(s) 66, 222, 354, 483
BseGI GGATG 5 cut(s) 102, 507, 535, 652, 754
BseLI CCNNNNNNNGG 3 cut(s) 253, 410, 745
BseMI GCAATG 1 cut(s) 859
BseMII CTCAG 1 cut(s) 508
BseNI ACTGG 1 cut(s) 98
BseRI GAGGAG 4 cut(s) 347, 584, 966, 1047
BseXI GCAGC 4 cut(s) 74, 229, 555, 955
BshFI GGCC 3 cut(s) 257, 414, 1038
BsiHKAI GWGCWC 1 cut(s) 617
BsiSI CCGG 3 cut(s) 66, 354, 707
BslFI GGGAC 2 cut(s) 385, 870
BslI CCNNNNNNNGG 3 cut(s) 253, 410, 745
BsmAI GTCTC 7 cut(s) 222, 566, 686, 728, 892, 1070, 1088
BsmBI CGTCTC 1 cut(s) 686
BsmFI GGGAC 2 cut(s) 385, 870
BsnI GGCC 3 cut(s) 257, 414, 1038
Bso31I GGTCTC 3 cut(s) 566, 728, 1070
Bsp119I TTCGAA 1 cut(s) 16
Bsp1286I GDGCHC 1 cut(s) 617
Bsp13I TCCGGA 1 cut(s) 706
Bsp1407I TGTACA 1 cut(s) 437
Bsp143I GATC 5 cut(s) 400, 559, 709, 766, 1030
BspACI CCGC 6 cut(s) 87, 150, 247, 318, 351, 986
BspANI GGCC 3 cut(s) 257, 414, 1038
BspCNI CTCAG 1 cut(s) 507
BspEI TCCGGA 1 cut(s) 706
BspHI TCATGA 2 cut(s) 712, 1128
BspLI GGNNCC 1 cut(s) 705
BspPI GGATC 3 cut(s) 395, 717, 761
BspQI GCTCTTC 1 cut(s) 283
BspT104I TTCGAA 1 cut(s) 16
BspTNI GGTCTC 3 cut(s) 566, 728, 1070
BsrDI GCAATG 1 cut(s) 859
BsrGI TGTACA 1 cut(s) 437
BsrI ACTGG 1 cut(s) 98
BssECI CCNNGG 4 cut(s) 66, 222, 354, 483
BssMI GATC 5 cut(s) 400, 559, 709, 766, 1030
BssT1I CCWWGG 1 cut(s) 222
Bst2UI CCWGG 1 cut(s) 251
Bst4CI ACNGT 3 cut(s) 328, 837, 1125
Bst6I CTCTTC 3 cut(s) 283, 603, 1068
BstAUI TGTACA 1 cut(s) 437
BstBI TTCGAA 1 cut(s) 16
BstC8I GCNNGC 1 cut(s) 247
BstDEI CTNAG 1 cut(s) 494
BstEII GGTNACC 1 cut(s) 152
BstF5I GGATG 5 cut(s) 102, 507, 535, 652, 754
BstHHI GCGC 1 cut(s) 543
BstKTI GATC 5 cut(s) 403, 562, 712, 769, 1033
BstMAI GTCTC 7 cut(s) 222, 566, 686, 728, 892, 1070, 1088
BstMBI GATC 5 cut(s) 400, 559, 709, 766, 1030
BstMWI GCNNNNNNNGC 2 cut(s) 62, 350
BstNI CCWGG 1 cut(s) 251
BstPI GGTNACC 1 cut(s) 152
BstSCI CCNGG 3 cut(s) 65, 249, 353
BstV1I GCAGC 4 cut(s) 74, 229, 555, 955
BstV2I GAAGAC 4 cut(s) 606, 879, 954, 1029
BstX2I RGATCY 1 cut(s) 400
BstYI RGATCY 1 cut(s) 400
BsuRI GGCC 3 cut(s) 257, 414, 1038
BtsCI GGATG 5 cut(s) 102, 507, 535, 652, 754
BtsI GCAGTG 2 cut(s) 690, 918
BtsIMutI CAGTG 3 cut(s) 690, 918, 1026
Cac8I GCNNGC 1 cut(s) 247
CciI TCATGA 2 cut(s) 712, 1128
CfoI GCGC 1 cut(s) 543
Cfr13I GGNCC 3 cut(s) 413, 704, 1037
Csp6I GTAC 2 cut(s) 79, 438
CviAII CATG 5 cut(s) 147, 214, 713, 1117, 1129
CviQI GTAC 2 cut(s) 79, 438
DdeI CTNAG 1 cut(s) 494
DpnI GATC 5 cut(s) 402, 561, 711, 768, 1032
DpnII GATC 5 cut(s) 400, 559, 709, 766, 1030
Eam1104I CTCTTC 3 cut(s) 283, 603, 1068
EarI CTCTTC 3 cut(s) 283, 603, 1068
EciI GGCGGA 1 cut(s) 1001
Eco130I CCWWGG 1 cut(s) 222
Eco31I GGTCTC 3 cut(s) 566, 728, 1070
Eco47I GGWCC 1 cut(s) 704
Eco57I CTGAAG 1 cut(s) 795
Eco91I GGTNACC 1 cut(s) 152
EcoO109I RGGNCCY 1 cut(s) 1037
EcoO65I GGTNACC 1 cut(s) 152
EcoRI GAATTC 1 cut(s) 407
EcoRII CCWGG 1 cut(s) 249
EcoT14I CCWWGG 1 cut(s) 222
ErhI CCWWGG 1 cut(s) 222
Esp3I CGTCTC 1 cut(s) 686
FaeI CATG 5 cut(s) 150, 217, 716, 1120, 1132
FalI AAGNNNNNCTT 2 cut(s) 265, 297
FaqI GGGAC 2 cut(s) 385, 870
FatI CATG 5 cut(s) 146, 213, 712, 1116, 1128
FauI CCCGC 1 cut(s) 254
FbaI TGATCA 1 cut(s) 559
Fnu4HI GCNGC 6 cut(s) 63, 88, 243, 351, 544, 969
FokI GGATG 5 cut(s) 89, 514, 542, 659, 761
Fsp4HI GCNGC 6 cut(s) 63, 88, 243, 351, 544, 969
FspBI CTAG 2 cut(s) 59, 347
FspI TGCGCA 1 cut(s) 542
GlaI GCGC 1 cut(s) 542
GluI GCNGC 6 cut(s) 63, 88, 243, 351, 544, 969
GsuI CTGGAG 1 cut(s) 272
HaeIII GGCC 3 cut(s) 257, 414, 1038
HapII CCGG 3 cut(s) 66, 354, 707
HhaI GCGC 1 cut(s) 543
Hin1II CATG 5 cut(s) 150, 217, 716, 1120, 1132
Hin6I GCGC 1 cut(s) 541
HinP1I GCGC 1 cut(s) 541
HincII GTYRAC 1 cut(s) 624
HindII GTYRAC 1 cut(s) 624
HinfI GANTC 3 cut(s) 13, 1132, 1149
HpaII CCGG 3 cut(s) 66, 354, 707
HphI GGTGA 1 cut(s) 164
Hpy166II GTNNAC 4 cut(s) 361, 438, 624, 905
Hpy188I TCNGA 8 cut(s) 141, 289, 484, 660, 937, 1012, 1093, 1137
Hpy188III TCNNGA 7 cut(s) 370, 395, 404, 496, 707, 713, 1129
Hpy8I GTNNAC 4 cut(s) 361, 438, 624, 905
Hpy99I CGWCG 1 cut(s) 871
HpyAV CCTTC 5 cut(s) 37, 226, 631, 682, 910
HpyCH4III ACNGT 3 cut(s) 328, 837, 1125
HpyCH4V TGCA 2 cut(s) 73, 944
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 350
HpyF3I CTNAG 1 cut(s) 494
Hsp92II CATG 5 cut(s) 150, 217, 716, 1120, 1132
HspAI GCGC 1 cut(s) 541
Kpn2I TCCGGA 1 cut(s) 706
Ksp22I TGATCA 1 cut(s) 559
Kzo9I GATC 5 cut(s) 400, 559, 709, 766, 1030
LguI GCTCTTC 1 cut(s) 283
LmnI GCTCC 4 cut(s) 898, 1048, 1054, 1060
Lsp1109I GCAGC 4 cut(s) 74, 229, 555, 955
LweI GCATC 2 cut(s) 520, 808
MaeI CTAG 2 cut(s) 59, 347
MaeIII GTNAC 2 cut(s) 128, 152
MalI GATC 5 cut(s) 402, 561, 711, 768, 1032
MboI GATC 5 cut(s) 400, 559, 709, 766, 1030
MflI RGATCY 1 cut(s) 400
MhlI GDGCHC 1 cut(s) 617
MluCI AATT 2 cut(s) 119, 407
MmeI TCCRAC 1 cut(s) 302
MroI TCCGGA 1 cut(s) 706
MseI TTAA 3 cut(s) 261, 552, 783
MspI CCGG 3 cut(s) 66, 354, 707
MspR9I CCNGG 3 cut(s) 67, 251, 355
MvaI CCWGG 1 cut(s) 251
MwoI GCNNNNNNNGC 2 cut(s) 62, 350
NciI CCSGG 2 cut(s) 67, 355
NdeII GATC 5 cut(s) 400, 559, 709, 766, 1030
NlaIII CATG 5 cut(s) 150, 217, 716, 1120, 1132
NlaIV GGNNCC 1 cut(s) 705
NmuCI GTSAC 1 cut(s) 152
NsbI TGCGCA 1 cut(s) 542
NspV TTCGAA 1 cut(s) 16
PagI TCATGA 2 cut(s) 712, 1128
PciSI GCTCTTC 1 cut(s) 283
PcsI WCGNNNNNNNCGW 1 cut(s) 402
PfeI GAWTC 3 cut(s) 13, 1132, 1149
PflFI GACNNNGTC 1 cut(s) 214
PkrI GCNGC 6 cut(s) 64, 89, 244, 352, 545, 970
PshBI ATTAAT 2 cut(s) 261, 552
Psp6I CCWGG 1 cut(s) 249
PspEI GGTNACC 1 cut(s) 152
PspGI CCWGG 1 cut(s) 249
PspN4I GGNNCC 1 cut(s) 705
PspPI GGNCC 3 cut(s) 413, 704, 1037
PsuI RGATCY 1 cut(s) 400
PsyI GACNNNGTC 1 cut(s) 214
RsaI GTAC 2 cut(s) 80, 439
RsaNI GTAC 2 cut(s) 79, 438
SapI GCTCTTC 1 cut(s) 283
SaqAI TTAA 3 cut(s) 261, 552, 783
SatI GCNGC 6 cut(s) 63, 88, 243, 351, 544, 969
Sau3AI GATC 5 cut(s) 400, 559, 709, 766, 1030
Sau96I GGNCC 3 cut(s) 413, 704, 1037
ScrFI CCNGG 3 cut(s) 67, 251, 355
SduI GDGCHC 1 cut(s) 617
SfaNI GCATC 2 cut(s) 520, 808
SfuI TTCGAA 1 cut(s) 16
SinI GGWCC 1 cut(s) 704
Sse9I AATT 2 cut(s) 119, 407
SsiI CCGC 6 cut(s) 87, 150, 247, 318, 351, 986
SspI AATATT 2 cut(s) 113, 1005
SspMI CTAG 2 cut(s) 59, 347
StyD4I CCNGG 3 cut(s) 65, 249, 353
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 3 cut(s) 328, 837, 1125
TaqI TCGA 5 cut(s) 16, 41, 396, 866, 1033
TaqII GACCGA 1 cut(s) 590
TasI AATT 2 cut(s) 119, 407
TatI WGTACW 1 cut(s) 437
TauI GCSGC 2 cut(s) 90, 353
TfiI GAWTC 3 cut(s) 13, 1132, 1149
Tru1I TTAA 3 cut(s) 261, 552, 783
Tru9I TTAA 3 cut(s) 261, 552, 783
TscAI CASTG 3 cut(s) 690, 918, 1033
TseFI GTSAC 1 cut(s) 152
TseI GCWGC 4 cut(s) 62, 242, 543, 968
Tsp45I GTSAC 1 cut(s) 152
TspDTI ATGAA 5 cut(s) 807, 824, 1117, 1145, 1158
TspGWI ACGGA 3 cut(s) 620, 710, 884
TspRI CASTG 3 cut(s) 690, 918, 1033
Tth111I GACNNNGTC 1 cut(s) 214
VpaK11BI GGWCC 1 cut(s) 704
VspI ATTAAT 2 cut(s) 261, 552
XapI RAATTY 2 cut(s) 119, 407
XspI CTAG 2 cut(s) 59, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.