Rroxscaffold_7G00200980

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0019524
Physical Location & Seq
Forward (+)
48453052 .. 48461977
8926 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00200980.1

Sequence Viewer

Length: 471 bp
ATGATGGACATGGATCATGGTGGACATTGGGCATTTCCCAACGAGTTTTTCCGCTTTCCGGGCTTGTTACGCTTGACAAGCACTTGTGGATCAAACATTGAGAAGCTTGCGGGGTTGCAACATCAAACATCATCTGAATTGGATCGAGTAAAAAACAAGAATTACTTTAAAACGCACAATAGTTTATCAAAACGCGCCTACACGCACTTGAGTAAAAATATTGTTTTTCCTATACTCGTCCCGCGGCGAGCCCTAACGACGCCGTCGTCGGAAGGGCCGTTTCGACCCGTTGGGTATGTTGTGAGGCATAGGGTCCTCGAGTATTGCGGTCTCGATCGATTTGGGAGATTGCGGTGGGCTGGTAGGGAGTTGAGGGACGAGGAAGATAGCGACAGCTGGTGGGTCTTGGATCGCTCTTCGCTGGCCCGGTTGGTGGATTGGATGGGTGCTGCGACGTCTAAGGTGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

156

Amino Acids

17.95

Weight (kDa)

9.63

Isoelectric Point (pI)

54.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 265
AatII GACGTC 1 cut(s) 458
AccII CGCG 2 cut(s) 195, 244
AciI CCGC 6 cut(s) 52, 110, 242, 244, 327, 352
AclWI GGATC 4 cut(s) 21, 97, 150, 417
AcyI GRCGYC 2 cut(s) 260, 455
AfiI CCNNNNNNNGG 2 cut(s) 58, 433
AjuI GAANNNNNNNTTGG 2 cut(s) 32, 64
AluBI AGCT 2 cut(s) 106, 396
AluI AGCT 2 cut(s) 106, 396
Alw26I GTCTC 1 cut(s) 335
AlwI GGATC 4 cut(s) 21, 97, 150, 417
Ama87I CYCGRG 1 cut(s) 317
AoxI GGCC 2 cut(s) 275, 423
ApeKI GCWGC 1 cut(s) 449
AspLEI GCGC 1 cut(s) 197
AspS9I GGNCC 3 cut(s) 275, 313, 424
AsuC2I CCSGG 2 cut(s) 60, 427
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 1 cut(s) 313
BanII GRGCYC 1 cut(s) 253
BbvI GCAGC 1 cut(s) 436
BccI CCATC 1 cut(s) 436
BceAI ACGGC 2 cut(s) 247, 262
BcnI CCSGG 2 cut(s) 60, 427
BcoDI GTCTC 1 cut(s) 335
BfaI CTAG 1 cut(s) 469
BisI GCNGC 2 cut(s) 245, 450
BlsI GCNGC 2 cut(s) 246, 451
Bme1390I CCNGG 2 cut(s) 60, 427
Bme18I GGWCC 1 cut(s) 313
BmeT110I CYCGRG 1 cut(s) 317
BmgT120I GGNCC 3 cut(s) 275, 313, 424
BmiI GGNNCC 1 cut(s) 314
BmrFI CCNGG 2 cut(s) 60, 427
BpuEI CTTGAG 1 cut(s) 229
BpuMI CCSGG 2 cut(s) 60, 427
Bsa29I ATCGAT 1 cut(s) 337
BsaHI GRCGYC 2 cut(s) 260, 455
BsaI GGTCTC 1 cut(s) 335
BsaJI CCNNGG 1 cut(s) 242
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 433
BseCI ATCGAT 1 cut(s) 337
BseDI CCNNGG 1 cut(s) 242
BseGI GGATG 1 cut(s) 447
BseLI CCNNNNNNNGG 2 cut(s) 58, 433
BseXI GCAGC 1 cut(s) 436
Bsh1236I CGCG 2 cut(s) 195, 244
Bsh1285I CGRYCG 1 cut(s) 337
BshFI GGCC 2 cut(s) 277, 425
BshVI ATCGAT 1 cut(s) 337
BsiEI CGRYCG 1 cut(s) 337
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 2 cut(s) 59, 427
BslFI GGGAC 2 cut(s) 224, 389
BslI CCNNNNNNNGG 2 cut(s) 58, 433
BsmAI GTCTC 1 cut(s) 335
BsmFI GGGAC 2 cut(s) 224, 389
BsnI GGCC 2 cut(s) 277, 425
Bso31I GGTCTC 1 cut(s) 335
BsoBI CYCGRG 1 cut(s) 317
Bsp1286I GDGCHC 1 cut(s) 253
Bsp143I GATC 5 cut(s) 13, 89, 142, 334, 409
BspACI CCGC 6 cut(s) 52, 110, 242, 244, 327, 352
BspANI GGCC 2 cut(s) 277, 425
BspDI ATCGAT 1 cut(s) 337
BspFNI CGCG 2 cut(s) 195, 244
BspLI GGNNCC 1 cut(s) 314
BspPI GGATC 4 cut(s) 21, 97, 150, 417
BspQI GCTCTTC 1 cut(s) 421
BspTNI GGTCTC 1 cut(s) 335
BssECI CCNNGG 1 cut(s) 242
BssMI GATC 5 cut(s) 13, 89, 142, 334, 409
BssNI GRCGYC 2 cut(s) 260, 455
Bst6I CTCTTC 1 cut(s) 421
BstACI GRCGYC 2 cut(s) 260, 455
BstC8I GCNNGC 3 cut(s) 108, 249, 423
BstDEI CTNAG 1 cut(s) 459
BstDSI CCRYGG 1 cut(s) 242
BstF5I GGATG 1 cut(s) 447
BstFNI CGCG 2 cut(s) 195, 244
BstHHI GCGC 1 cut(s) 197
BstKTI GATC 5 cut(s) 16, 92, 145, 337, 412
BstMAI GTCTC 1 cut(s) 335
BstMBI GATC 5 cut(s) 13, 89, 142, 334, 409
BstMCI CGRYCG 1 cut(s) 337
BstMWI GCNNNNNNNGC 3 cut(s) 60, 69, 78
BstSCI CCNGG 2 cut(s) 58, 425
BstUI CGCG 2 cut(s) 195, 244
BstV1I GCAGC 1 cut(s) 436
Bsu15I ATCGAT 1 cut(s) 337
BsuRI GGCC 2 cut(s) 277, 425
BsuTUI ATCGAT 1 cut(s) 337
BtgI CCRYGG 1 cut(s) 242
BtsCI GGATG 1 cut(s) 447
Cac8I GCNNGC 3 cut(s) 108, 249, 423
CfoI GCGC 1 cut(s) 197
Cfr13I GGNCC 3 cut(s) 275, 313, 424
Cfr42I CCGCGG 1 cut(s) 245
ClaI ATCGAT 1 cut(s) 337
CseI GACGC 1 cut(s) 268
CviAII CATG 2 cut(s) 10, 17
CviJI RGCY 8 cut(s) 63, 106, 251, 277, 359, 396, 425, 468
CviKI_1 RGCY 8 cut(s) 63, 106, 251, 277, 359, 396, 425, 468
DdeI CTNAG 1 cut(s) 459
DpnI GATC 5 cut(s) 15, 91, 144, 336, 411
DpnII GATC 5 cut(s) 13, 89, 142, 334, 409
DraI TTTAAA 1 cut(s) 169
DrdI GACNNNNNNGTC 1 cut(s) 265
DseDI GACNNNNNNGTC 1 cut(s) 265
Eam1104I CTCTTC 1 cut(s) 421
EarI CTCTTC 1 cut(s) 421
Eco24I GRGCYC 1 cut(s) 253
Eco31I GGTCTC 1 cut(s) 335
Eco47I GGWCC 1 cut(s) 313
Eco88I CYCGRG 1 cut(s) 317
EcoO109I RGGNCCY 1 cut(s) 313
EcoT38I GRGCYC 1 cut(s) 253
FaeI CATG 2 cut(s) 13, 20
FaiI YATR 5 cut(s) 11, 18, 233, 297, 309
FalI AAGNNNNNCTT 2 cut(s) 149, 181
FaqI GGGAC 2 cut(s) 224, 389
FatI CATG 2 cut(s) 9, 16
FauI CCCGC 2 cut(s) 103, 249
Fnu4HI GCNGC 2 cut(s) 245, 450
FokI GGATG 1 cut(s) 454
FriOI GRGCYC 1 cut(s) 253
Fsp4HI GCNGC 2 cut(s) 245, 450
FspBI CTAG 1 cut(s) 469
GlaI GCGC 1 cut(s) 196
GluI GCNGC 2 cut(s) 245, 450
HaeIII GGCC 2 cut(s) 277, 425
HapII CCGG 2 cut(s) 59, 427
HgaI GACGC 1 cut(s) 268
HhaI GCGC 1 cut(s) 197
Hin1I GRCGYC 2 cut(s) 260, 455
Hin1II CATG 2 cut(s) 13, 20
Hin6I GCGC 1 cut(s) 195
HinP1I GCGC 1 cut(s) 195
HindIII AAGCTT 1 cut(s) 104
HpaII CCGG 2 cut(s) 59, 427
Hpy166II GTNNAC 1 cut(s) 23
Hpy188I TCNGA 2 cut(s) 136, 271
Hpy188III TCNNGA 1 cut(s) 332
Hpy8I GTNNAC 1 cut(s) 23
Hpy99I CGWCG 4 cut(s) 262, 268, 271, 457
HpyAV CCTTC 1 cut(s) 266
HpyCH4IV ACGT 1 cut(s) 455
HpyCH4V TGCA 1 cut(s) 118
HpyF10VI GCNNNNNNNGC 3 cut(s) 60, 69, 78
HpyF3I CTNAG 1 cut(s) 459
HpySE526I ACGT 1 cut(s) 455
Hsp92I GRCGYC 2 cut(s) 260, 455
Hsp92II CATG 2 cut(s) 13, 20
HspAI GCGC 1 cut(s) 195
KspI CCGCGG 1 cut(s) 245
Kzo9I GATC 5 cut(s) 13, 89, 142, 334, 409
LguI GCTCTTC 1 cut(s) 421
LpnPI CCDG 5 cut(s) 72, 345, 382, 407, 440
Lsp1109I GCAGC 1 cut(s) 436
MaeI CTAG 1 cut(s) 469
MaeII ACGT 1 cut(s) 455
MaeIII GTNAC 1 cut(s) 66
MalI GATC 5 cut(s) 15, 91, 144, 336, 411
MboI GATC 5 cut(s) 13, 89, 142, 334, 409
MboII GAAGA 2 cut(s) 395, 408
MhlI GDGCHC 1 cut(s) 253
MluCI AATT 2 cut(s) 137, 160
MmeI TCCRAC 1 cut(s) 249
MnlI CCTC 4 cut(s) 297, 326, 366, 373
MseI TTAA 1 cut(s) 168
MspA1I CMGCKG 2 cut(s) 244, 396
MspI CCGG 2 cut(s) 59, 427
MspR9I CCNGG 2 cut(s) 60, 427
MvnI CGCG 2 cut(s) 195, 244
MwoI GCNNNNNNNGC 3 cut(s) 60, 69, 78
NciI CCSGG 2 cut(s) 60, 427
NdeII GATC 5 cut(s) 13, 89, 142, 334, 409
NlaIII CATG 2 cut(s) 13, 20
NlaIV GGNNCC 1 cut(s) 314
PaeR7I CTCGAG 1 cut(s) 317
PciSI GCTCTTC 1 cut(s) 421
PcsI WCGNNNNNNNCGW 2 cut(s) 263, 275
PflFI GACNNNGTC 1 cut(s) 262
PkrI GCNGC 2 cut(s) 246, 451
Ple19I CGATCG 1 cut(s) 337
PpuMI RGGWCCY 1 cut(s) 313
Psp5II RGGWCCY 1 cut(s) 313
PspN4I GGNNCC 1 cut(s) 314
PspPI GGNCC 3 cut(s) 275, 313, 424
PspPPI RGGWCCY 1 cut(s) 313
PspXI VCTCGAGB 1 cut(s) 317
PsyI GACNNNGTC 1 cut(s) 262
PvuI CGATCG 1 cut(s) 337
PvuII CAGCTG 1 cut(s) 396
SacII CCGCGG 1 cut(s) 245
SapI GCTCTTC 1 cut(s) 421
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 2 cut(s) 245, 450
Sau3AI GATC 5 cut(s) 13, 89, 142, 334, 409
Sau96I GGNCC 3 cut(s) 275, 313, 424
ScrFI CCNGG 2 cut(s) 60, 427
SduI GDGCHC 1 cut(s) 253
SetI ASST 4 cut(s) 108, 398, 458, 465
Sfr274I CTCGAG 1 cut(s) 317
Sfr303I CCGCGG 1 cut(s) 245
SgrBI CCGCGG 1 cut(s) 245
SinI GGWCC 1 cut(s) 313
SlaI CTCGAG 1 cut(s) 317
SmlI CTYRAG 2 cut(s) 208, 317
SmoI CTYRAG 2 cut(s) 208, 317
Sse9I AATT 2 cut(s) 137, 160
SsiI CCGC 6 cut(s) 52, 110, 242, 244, 327, 352
SspI AATATT 1 cut(s) 220
SspMI CTAG 1 cut(s) 469
StyD4I CCNGG 2 cut(s) 58, 425
TaiI ACGT 1 cut(s) 458
TaqI TCGA 5 cut(s) 145, 283, 318, 333, 337
TasI AATT 2 cut(s) 137, 160
TauI GCSGC 1 cut(s) 247
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TseI GCWGC 1 cut(s) 449
Tth111I GACNNNGTC 1 cut(s) 262
VpaK11BI GGWCC 1 cut(s) 313
XhoI CTCGAG 1 cut(s) 317
XspI CTAG 1 cut(s) 469
ZraI GACGTC 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.