Rroxscaffold_4G00304110

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0019524
Physical Location & Seq
Forward (+)
24781458 .. 24782039
582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00304110.1

Sequence Viewer

Length: 582 bp
ATGCCGAGCTTACTCTCCAAGAGATCATCTGCTGAAGTTGTTAGGAGATTTGGTCACCTTCCACCAAGATTACTTCTCCGGAGTCTCCAAGCATTGCACAAGAAGCTACTTTTGGGTGAAGCATTTCAAATTGAAAAGAATGCAGCAGATCGGGTGGAAGATAGATATTGGCATTGTGACGATGAAATTATGACCGTTGTCCGGACTATGGTTCGCATGTGTGGCTCGAAGATGCAAATAGCATTAGAGTTTTGGTCTCTCGATTTCATATCTAGGGTTGGTATTGGGGATCCACATTGTGTTGTTTTCCATCTCGCCAGAAACTCGAGTAACATCCATGCGTGCCAACTAGTCAATCAAGGCTTTTCTACAGAGGGCACATTTCCAATTGATACCGGATTGTCGAAGAACTTCGGTCGCACTTCGAAACTTCTAAAGAAGTATTTGCGGATCATGGTGGACACGGATCATGGTGGACATTGGGCATTTCCCAACGAGGTGACTTATACTTCATTGATAATTTTTGGAATGTTTAGTTTTGACAAGTTGACAAATAAAGAAGAGTGGAAATTGTTGTTGTGA

Protein Analysis

193

Amino Acids

22.16

Weight (kDa)

9.12

Isoelectric Point (pI)

37.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 78, 201
AciI CCGC 1 cut(s) 448
AclWI GGATC 4 cut(s) 284, 297, 458, 474
AcuI CTGAAG 1 cut(s) 54
AdeI CACNNNGTG 1 cut(s) 299
AfiI CCNNNNNNNGG 2 cut(s) 201, 208
AgsI TTSAA 2 cut(s) 128, 134
AhlI ACTAGT 1 cut(s) 349
AjuI GAANNNNNNNTTGG 2 cut(s) 95, 127
AluBI AGCT 2 cut(s) 9, 106
AluI AGCT 2 cut(s) 9, 106
Alw26I GTCTC 2 cut(s) 89, 261
AlwI GGATC 4 cut(s) 284, 297, 458, 474
Ama87I CYCGRG 1 cut(s) 325
Aor13HI TCCGGA 2 cut(s) 78, 201
ApeKI GCWGC 1 cut(s) 143
Asp700I GAANNNNTTC 2 cut(s) 123, 410
AsuHPI GGTGA 3 cut(s) 47, 128, 511
AsuII TTCGAA 1 cut(s) 425
AvaI CYCGRG 1 cut(s) 325
BaeGI GKGCMC 1 cut(s) 380
BamHI GGATCC 1 cut(s) 289
BbvI GCAGC 1 cut(s) 155
BccI CCATC 1 cut(s) 318
BcoDI GTCTC 2 cut(s) 89, 261
BcuI ACTAGT 1 cut(s) 349
BfaI CTAG 2 cut(s) 273, 350
BfmI CTRYAG 1 cut(s) 369
BisI GCNGC 1 cut(s) 144
BlsI GCNGC 1 cut(s) 145
BmeT110I CYCGRG 1 cut(s) 325
BmiI GGNNCC 1 cut(s) 291
BmsI GCATC 1 cut(s) 222
BoxI GACNNNNGTC 1 cut(s) 197
Bpu14I TTCGAA 1 cut(s) 425
BsaI GGTCTC 1 cut(s) 261
BsaWI WCCGGW 3 cut(s) 78, 201, 395
BsaXI ACNNNNNCTCC 2 cut(s) 37, 67
Bsc4I CCNNNNNNNGG 2 cut(s) 201, 208
Bse3DI GCAATG 1 cut(s) 92
BseAI TCCGGA 2 cut(s) 78, 201
BseGI GGATG 1 cut(s) 333
BseLI CCNNNNNNNGG 2 cut(s) 201, 208
BseMI GCAATG 1 cut(s) 92
BseSI GKGCMC 1 cut(s) 380
BseXI GCAGC 1 cut(s) 155
Bsh1285I CGRYCG 1 cut(s) 418
BsiEI CGRYCG 1 cut(s) 418
BsiHKCI CYCGRG 1 cut(s) 325
BsiSI CCGG 3 cut(s) 79, 202, 396
BslI CCNNNNNNNGG 2 cut(s) 201, 208
BsmAI GTCTC 2 cut(s) 89, 261
BsmI GAATGC 1 cut(s) 145
Bso31I GGTCTC 1 cut(s) 261
BsoBI CYCGRG 1 cut(s) 325
Bsp119I TTCGAA 1 cut(s) 425
Bsp1286I GDGCHC 1 cut(s) 380
Bsp13I TCCGGA 2 cut(s) 78, 201
Bsp143I GATC 5 cut(s) 23, 148, 289, 450, 466
BspACI CCGC 1 cut(s) 448
BspEI TCCGGA 2 cut(s) 78, 201
BspLI GGNNCC 1 cut(s) 291
BspPI GGATC 4 cut(s) 284, 297, 458, 474
BspT104I TTCGAA 1 cut(s) 425
BspTNI GGTCTC 1 cut(s) 261
BsrDI GCAATG 1 cut(s) 92
BssMI GATC 5 cut(s) 23, 148, 289, 450, 466
Bst4CI ACNGT 1 cut(s) 196
Bst6I CTCTTC 1 cut(s) 555
BstBI TTCGAA 1 cut(s) 425
BstC8I GCNNGC 1 cut(s) 343
BstEII GGTNACC 1 cut(s) 53
BstF5I GGATG 1 cut(s) 333
BstKTI GATC 5 cut(s) 26, 151, 292, 453, 469
BstMAI GTCTC 2 cut(s) 89, 261
BstMBI GATC 5 cut(s) 23, 148, 289, 450, 466
BstMCI CGRYCG 1 cut(s) 418
BstMWI GCNNNNNNNGC 2 cut(s) 103, 222
BstNSI RCATGY 1 cut(s) 220
BstPAI GACNNNNGTC 1 cut(s) 197
BstPI GGTNACC 1 cut(s) 53
BstSFI CTRYAG 1 cut(s) 369
BstSLI GKGCMC 1 cut(s) 380
BstV1I GCAGC 1 cut(s) 155
BstX2I RGATCY 1 cut(s) 289
BstYI RGATCY 1 cut(s) 289
BtsCI GGATG 1 cut(s) 333
Cac8I GCNNGC 1 cut(s) 343
CviAII CATG 4 cut(s) 217, 338, 454, 470
CviJI RGCY 4 cut(s) 9, 106, 225, 363
CviKI_1 RGCY 4 cut(s) 9, 106, 225, 363
DpnI GATC 5 cut(s) 25, 150, 291, 452, 468
DpnII GATC 5 cut(s) 23, 148, 289, 450, 466
DraIII CACNNNGTG 1 cut(s) 299
Eam1104I CTCTTC 1 cut(s) 555
EarI CTCTTC 1 cut(s) 555
Eco31I GGTCTC 1 cut(s) 261
Eco57I CTGAAG 1 cut(s) 54
Eco88I CYCGRG 1 cut(s) 325
Eco91I GGTNACC 1 cut(s) 53
EcoO65I GGTNACC 1 cut(s) 53
FaeI CATG 4 cut(s) 220, 341, 457, 473
FaiI YATR 8 cut(s) 191, 209, 218, 269, 339, 455, 471, 507
FatI CATG 4 cut(s) 216, 337, 453, 469
Fnu4HI GCNGC 1 cut(s) 144
FokI GGATG 1 cut(s) 320
Fsp4HI GCNGC 1 cut(s) 144
FspBI CTAG 2 cut(s) 273, 350
GluI GCNGC 1 cut(s) 144
HapII CCGG 3 cut(s) 79, 202, 396
Hin1II CATG 4 cut(s) 220, 341, 457, 473
HincII GTYRAC 1 cut(s) 549
HindII GTYRAC 1 cut(s) 549
HinfI GANTC 1 cut(s) 82
HpaII CCGG 3 cut(s) 79, 202, 396
HphI GGTGA 3 cut(s) 47, 128, 511
Hpy166II GTNNAC 3 cut(s) 460, 476, 549
Hpy188III TCNNGA 3 cut(s) 79, 202, 260
Hpy8I GTNNAC 3 cut(s) 460, 476, 549
HpyAV CCTTC 1 cut(s) 68
HpyCH4III ACNGT 1 cut(s) 196
HpyCH4V TGCA 3 cut(s) 97, 143, 235
HpyF10VI GCNNNNNNNGC 2 cut(s) 103, 222
Hsp92II CATG 4 cut(s) 220, 341, 457, 473
Kpn2I TCCGGA 2 cut(s) 78, 201
Kzo9I GATC 5 cut(s) 23, 148, 289, 450, 466
LpnPI CCDG 4 cut(s) 92, 215, 331, 409
Lsp1109I GCAGC 1 cut(s) 155
LweI GCATC 1 cut(s) 222
MaeI CTAG 2 cut(s) 273, 350
MaeIII GTNAC 4 cut(s) 53, 176, 329, 499
MalI GATC 5 cut(s) 25, 150, 291, 452, 468
MboI GATC 5 cut(s) 23, 148, 289, 450, 466
MboII GAAGA 4 cut(s) 170, 241, 418, 572
MfeI CAATTG 1 cut(s) 387
MflI RGATCY 1 cut(s) 289
MhlI GDGCHC 1 cut(s) 380
MluCI AATT 5 cut(s) 129, 186, 387, 519, 569
MlyI GAGTC 1 cut(s) 91
MnlI CCTC 2 cut(s) 367, 490
MroI TCCGGA 2 cut(s) 78, 201
MroXI GAANNNNTTC 2 cut(s) 123, 410
MspI CCGG 3 cut(s) 79, 202, 396
MunI CAATTG 1 cut(s) 387
Mva1269I GAATGC 1 cut(s) 145
MwoI GCNNNNNNNGC 2 cut(s) 103, 222
NdeII GATC 5 cut(s) 23, 148, 289, 450, 466
NlaIII CATG 4 cut(s) 220, 341, 457, 473
NlaIV GGNNCC 1 cut(s) 291
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 3 cut(s) 53, 176, 499
NspI RCATGY 1 cut(s) 220
NspV TTCGAA 1 cut(s) 425
PaeR7I CTCGAG 1 cut(s) 325
PctI GAATGC 1 cut(s) 145
PdmI GAANNNNTTC 2 cut(s) 123, 410
PkrI GCNGC 1 cut(s) 145
PleI GAGTC 1 cut(s) 90
PpsI GAGTC 1 cut(s) 90
PshAI GACNNNNGTC 1 cut(s) 197
PspEI GGTNACC 1 cut(s) 53
PspN4I GGNNCC 1 cut(s) 291
PspXI VCTCGAGB 1 cut(s) 325
PsuI RGATCY 1 cut(s) 289
SatI GCNGC 1 cut(s) 144
Sau3AI GATC 5 cut(s) 23, 148, 289, 450, 466
SchI GAGTC 1 cut(s) 91
SduI GDGCHC 1 cut(s) 380
SetI ASST 4 cut(s) 11, 60, 108, 501
SfaNI GCATC 1 cut(s) 222
SfcI CTRYAG 1 cut(s) 369
Sfr274I CTCGAG 1 cut(s) 325
SfuI TTCGAA 1 cut(s) 425
SlaI CTCGAG 1 cut(s) 325
SmlI CTYRAG 1 cut(s) 325
SmoI CTYRAG 1 cut(s) 325
SpeI ACTAGT 1 cut(s) 349
Sse9I AATT 5 cut(s) 129, 186, 387, 519, 569
SsiI CCGC 1 cut(s) 448
SspMI CTAG 2 cut(s) 273, 350
TaaI ACNGT 1 cut(s) 196
TaqI TCGA 5 cut(s) 227, 261, 326, 404, 425
TaqII GACCGA 1 cut(s) 404
TasI AATT 5 cut(s) 129, 186, 387, 519, 569
TseFI GTSAC 3 cut(s) 53, 176, 499
TseI GCWGC 1 cut(s) 143
Tsp45I GTSAC 3 cut(s) 53, 176, 499
TspDTI ATGAA 3 cut(s) 198, 256, 501
TspGWI ACGGA 1 cut(s) 479
XceI RCATGY 1 cut(s) 220
XhoI CTCGAG 1 cut(s) 325
XmnI GAANNNNTTC 2 cut(s) 123, 410
XspI CTAG 2 cut(s) 273, 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.