Rmu_sc0008647.1_g000021

GAG-pre-integrase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008647.1
OG0018620
Physical Location & Seq
Reverse (-)
94679 .. 95437
759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008647.1_g000021.1.cds

Sequence Viewer

Length: 759 bp
atggaacgttttaaaactgttcgggaccaactctctgcttctggagttttcattcctgatgaagatatagtgtttctaatcctaaatggattgcctattgactttgcggccattaagactatcatcagagttcgaacaacgccaatatctctaagcgagcttcgcacaatgttattggatgttgagtccgatatggttcaagaatcaagatcatttgctgctacttccatgctccaacacaaagcactaccatcgtcttctcttacagctaagattgcacaaaatattcttgccaattcatctcaaagtatgcaatatgggataacaagtacatggccatcatctcaagcaatctttccatcccattatactggatattcttcaatgacacattctctacaaactgctgcatcaaatgttggtttcttgcctaatgttaccaatactaatctgattccaggtataccttcttataaccattacaatacatcatctcgtgcctccaatgttcatcattataatgctacaccacctagctctactacacattttgggataccacataatagcatgatatcgagttcagctcttagtatgcctaatgctcctccaaattgtcaagtcctgctcccattcactaccaatgttggtgttccgctgtcagtgcctaattatggatatatgttgcataataatgttggaaacgcttgtgagtctacgaatttatggcgattacttcctctcattcctcctggttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 gag_pre-integrs rve

Protein Analysis

252

Amino Acids

27.38

Weight (kDa)

7.13

Isoelectric Point (pI)

48.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 474, 519
AccI GTMKAC 2 cut(s) 463, 716
AciI CCGC 2 cut(s) 107, 656
AclI AACGTT 1 cut(s) 7
AcoI YGGCCR 2 cut(s) 108, 335
AcsI RAATTY 1 cut(s) 721
AfaI GTAC 1 cut(s) 331
AfiI CCNNNNNNNGG 1 cut(s) 674
AgsI TTSAA 2 cut(s) 200, 384
AjnI CCWGG 2 cut(s) 457, 751
AluBI AGCT 4 cut(s) 160, 269, 537, 587
AluI AGCT 4 cut(s) 160, 269, 537, 587
AoxI GGCC 2 cut(s) 108, 335
ApeKI GCWGC 2 cut(s) 218, 407
ApoI RAATTY 1 cut(s) 721
AspS9I GGNCC 1 cut(s) 25
AsuII TTCGAA 1 cut(s) 133
AvaII GGWCC 1 cut(s) 25
BalI TGGCCA 1 cut(s) 337
BauI CACGAG 1 cut(s) 495
BbsI GAAGAC 1 cut(s) 249
BbvI GCAGC 2 cut(s) 205, 394
BccI CCATC 3 cut(s) 259, 346, 367
BcgI CGANNNNNNTGC 2 cut(s) 234, 268
BciT130I CCWGG 2 cut(s) 459, 753
BciVI GTATCC 1 cut(s) 549
BfaI CTAG 1 cut(s) 534
BfuI GTATCC 1 cut(s) 549
BisI GCNGC 3 cut(s) 108, 219, 408
BlsI GCNGC 3 cut(s) 109, 220, 409
Bme1390I CCNGG 2 cut(s) 459, 753
Bme18I GGWCC 1 cut(s) 25
BmgT120I GGNCC 1 cut(s) 25
BmiI GGNNCC 1 cut(s) 26
BmrFI CCNGG 2 cut(s) 459, 753
BmsI GCATC 1 cut(s) 419
BpiI GAAGAC 1 cut(s) 249
BplI GAGNNNNNCTC 2 cut(s) 571, 603
BpmI CTGGAG 1 cut(s) 63
Bpu14I TTCGAA 1 cut(s) 133
BpuEI CTTGAG 1 cut(s) 330
Bsc4I CCNNNNNNNGG 1 cut(s) 674
Bse1I ACTGG 1 cut(s) 376
BseBI CCWGG 2 cut(s) 459, 753
BseGI GGATG 2 cut(s) 184, 359
BseLI CCNNNNNNNGG 1 cut(s) 674
BseNI ACTGG 1 cut(s) 376
BseRI GAGGAG 1 cut(s) 597
BseXI GCAGC 2 cut(s) 205, 394
BshFI GGCC 2 cut(s) 110, 337
BslFI GGGAC 1 cut(s) 38
BslI CCNNNNNNNGG 1 cut(s) 674
BsmFI GGGAC 1 cut(s) 38
BsnI GGCC 2 cut(s) 110, 337
Bsp119I TTCGAA 1 cut(s) 133
Bsp143I GATC 1 cut(s) 209
BspACI CCGC 2 cut(s) 107, 656
BspANI GGCC 2 cut(s) 110, 337
BspLI GGNNCC 1 cut(s) 26
BspT104I TTCGAA 1 cut(s) 133
BsrI ACTGG 1 cut(s) 376
BssMI GATC 1 cut(s) 209
BssNAI GTATAC 1 cut(s) 464
BssSI CACGAG 1 cut(s) 495
Bst1107I GTATAC 1 cut(s) 464
Bst2BI CACGAG 1 cut(s) 495
Bst2UI CCWGG 2 cut(s) 459, 753
Bst4CI ACNGT 1 cut(s) 19
BstBI TTCGAA 1 cut(s) 133
BstC8I GCNNGC 1 cut(s) 158
BstDEI CTNAG 3 cut(s) 152, 270, 590
BstF5I GGATG 2 cut(s) 184, 359
BstKTI GATC 1 cut(s) 212
BstMBI GATC 1 cut(s) 209
BstMWI GCNNNNNNNGC 3 cut(s) 162, 275, 664
BstNI CCWGG 2 cut(s) 459, 753
BstSCI CCNGG 2 cut(s) 457, 751
BstV1I GCAGC 2 cut(s) 205, 394
BstV2I GAAGAC 1 cut(s) 249
BstXI CCANNNNNNTGG 1 cut(s) 371
BstZ17I GTATAC 1 cut(s) 464
BsuI GTATCC 1 cut(s) 549
BsuRI GGCC 2 cut(s) 110, 337
BtsCI GGATG 2 cut(s) 184, 359
BtsIMutI CAGTG 1 cut(s) 669
Cac8I GCNNGC 1 cut(s) 158
Cfr13I GGNCC 1 cut(s) 25
Csp6I GTAC 1 cut(s) 330
CviAII CATG 3 cut(s) 229, 333, 571
CviJI RGCY 6 cut(s) 110, 160, 269, 337, 537, 587
CviKI_1 RGCY 6 cut(s) 110, 160, 269, 337, 537, 587
CviQI GTAC 1 cut(s) 330
DdeI CTNAG 3 cut(s) 152, 270, 590
DpnI GATC 1 cut(s) 211
DpnII GATC 1 cut(s) 209
DraI TTTAAA 1 cut(s) 13
EaeI YGGCCR 2 cut(s) 108, 335
Eco32I GATATC 1 cut(s) 576
Eco47I GGWCC 1 cut(s) 25
EcoRII CCWGG 2 cut(s) 457, 751
EcoRV GATATC 1 cut(s) 576
FaeI CATG 3 cut(s) 232, 336, 574
FaqI GGGAC 1 cut(s) 38
FatI CATG 3 cut(s) 228, 332, 570
FblI GTMKAC 2 cut(s) 463, 716
Fnu4HI GCNGC 3 cut(s) 108, 219, 408
FokI GGATG 2 cut(s) 191, 346
Fsp4HI GCNGC 3 cut(s) 108, 219, 408
FspBI CTAG 1 cut(s) 534
GluI GCNGC 3 cut(s) 108, 219, 408
GsuI CTGGAG 1 cut(s) 63
HaeIII GGCC 2 cut(s) 110, 337
Hin1II CATG 3 cut(s) 232, 336, 574
HinfI GANTC 4 cut(s) 185, 203, 454, 713
Hpy166II GTNNAC 2 cut(s) 464, 717
Hpy188I TCNGA 3 cut(s) 128, 190, 453
Hpy188III TCNNGA 5 cut(s) 23, 42, 56, 200, 207
Hpy8I GTNNAC 2 cut(s) 464, 717
HpyAV CCTTC 1 cut(s) 477
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4IV ACGT 1 cut(s) 7
HpyCH4V TGCA 4 cut(s) 278, 313, 410, 688
HpyF10VI GCNNNNNNNGC 3 cut(s) 162, 275, 664
HpyF3I CTNAG 3 cut(s) 152, 270, 590
HpySE526I ACGT 1 cut(s) 7
Hsp92II CATG 3 cut(s) 232, 336, 574
Kzo9I GATC 1 cut(s) 209
LmnI GCTCC 3 cut(s) 237, 610, 633
LpnPI CCDG 7 cut(s) 27, 69, 357, 444, 471, 638, 738
Lsp1109I GCAGC 2 cut(s) 205, 394
LweI GCATC 1 cut(s) 419
MaeI CTAG 1 cut(s) 534
MaeII ACGT 1 cut(s) 7
MaeIII GTNAC 1 cut(s) 436
MalI GATC 1 cut(s) 211
MboI GATC 1 cut(s) 209
MboII GAAGA 3 cut(s) 74, 249, 372
MlsI TGGCCA 1 cut(s) 337
MluCI AATT 4 cut(s) 295, 613, 670, 721
MluNI TGGCCA 1 cut(s) 337
MlyI GAGTC 2 cut(s) 194, 722
MmeI TCCRAC 2 cut(s) 259, 679
MnlI CCTC 4 cut(s) 511, 618, 750, 759
Mox20I TGGCCA 1 cut(s) 337
MscI TGGCCA 1 cut(s) 337
MseI TTAA 2 cut(s) 12, 114
MslI CAYNNNNRTG 2 cut(s) 519, 693
Msp20I TGGCCA 1 cut(s) 337
MspA1I CMGCKG 1 cut(s) 658
MspR9I CCNGG 2 cut(s) 459, 753
MvaI CCWGG 2 cut(s) 459, 753
MwoI GCNNNNNNNGC 3 cut(s) 162, 275, 664
NdeII GATC 1 cut(s) 209
NlaIII CATG 3 cut(s) 232, 336, 574
NlaIV GGNNCC 1 cut(s) 26
NspV TTCGAA 1 cut(s) 133
PfeI GAWTC 2 cut(s) 203, 454
PkrI GCNGC 3 cut(s) 109, 220, 409
PleI GAGTC 2 cut(s) 193, 721
PpsI GAGTC 2 cut(s) 193, 721
PsiI TTATAA 2 cut(s) 474, 519
Psp1406I AACGTT 1 cut(s) 7
Psp6I CCWGG 2 cut(s) 457, 751
PspGI CCWGG 2 cut(s) 457, 751
PspN4I GGNNCC 1 cut(s) 26
PspPI GGNCC 1 cut(s) 25
RsaI GTAC 1 cut(s) 331
RsaNI GTAC 1 cut(s) 330
RseI CAYNNNNRTG 2 cut(s) 519, 693
SaqAI TTAA 2 cut(s) 12, 114
SatI GCNGC 3 cut(s) 108, 219, 408
Sau3AI GATC 1 cut(s) 209
Sau96I GGNCC 1 cut(s) 25
SchI GAGTC 2 cut(s) 194, 722
ScrFI CCNGG 2 cut(s) 459, 753
SetI ASST 8 cut(s) 10, 162, 271, 463, 469, 535, 539, 589
SfaNI GCATC 1 cut(s) 419
SfuI TTCGAA 1 cut(s) 133
SinI GGWCC 1 cut(s) 25
SmiMI CAYNNNNRTG 2 cut(s) 519, 693
SmlI CTYRAG 1 cut(s) 345
SmoI CTYRAG 1 cut(s) 345
Sse9I AATT 4 cut(s) 295, 613, 670, 721
SsiI CCGC 2 cut(s) 107, 656
SspI AATATT 1 cut(s) 286
SspMI CTAG 1 cut(s) 534
StyD4I CCNGG 2 cut(s) 457, 751
TaaI ACNGT 1 cut(s) 19
TaiI ACGT 1 cut(s) 10
TaqI TCGA 2 cut(s) 133, 578
TasI AATT 4 cut(s) 295, 613, 670, 721
TatI WGTACW 1 cut(s) 329
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 2 cut(s) 203, 454
Tru1I TTAA 2 cut(s) 12, 114
Tru9I TTAA 2 cut(s) 12, 114
TscAI CASTG 1 cut(s) 669
TseI GCWGC 2 cut(s) 218, 407
TspDTI ATGAA 4 cut(s) 40, 75, 288, 500
TspRI CASTG 1 cut(s) 669
VpaK11BI GGWCC 1 cut(s) 25
XapI RAATTY 1 cut(s) 721
XmiI GTMKAC 2 cut(s) 463, 716
XspI CTAG 1 cut(s) 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.