MD15G1050800.v1.1
MYB Family

Trihelix transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015554
Physical Location & Seq
Forward (+)
3467319 .. 3469513
2195 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1050800.v1.1.491

Sequence Viewer

Length: 1686 bp
ATGCATTCGAATTTTGGAGCTGCAGAGATTGGAAATCAGCACTTTATGGATTCTAATTCATCAGTTTTACCAGTCTTCAATCCTCAGAAGCAAAACCAGAACTTTTTGCATCAACAATTTCATCCGCAGCAGCCGCAGCCATGTTATCATCTCAGTCAATTAGCTCATCCGATTCCGATTACCCACGACTTGTTCCAACGACAGAATCAGCTCCAATTCCAAGCATTGCTGCAGCAGCAGCAGCAGGAACAGCAGGAAGAAGGCAGGCTGCCATGGCAGCCGAGCCTTCCAAGTTTCAAGCTTGGTCTCAGTGAAAGCAGTGGACCACATAACTTAGGATTCAAAAATTGGAAAACCCAAGAATATTACGGGATTAGAAGAGAATCAATGTGTTGGAAGCCTCTTGACGCGGTAGTCGCCGACACAACAAACAAAAGGCAGAGGCAGGAAGAAGGGGAGAGTTGCAGAGACTTGGAGGGAAAACACAGGCTTTATGGTGAGCTTGAAGCAATCTATAACCTAGCAAAGATGGGGGAGGCTAATCAGAATCAAACCGGCTCTGGCTCTGCGCTCACCGGCGAAAACTGCTCGAAAAATGTGGAATTTCCTGTGGTTTTTGTTGATCCTAATGGCCTCAATGCTGCTCCAGCTGATAATGTTAGGGTGGATAATGTGTCGGAAGCGTCCATTGGAGAGGAGCGTTCAGTGGGGAAGATTCAGAAGAGGAAGAGAAAGTCGAAAATGAAGGAGCAATTAAGTTCAATGACTAGGTTTTTCGAGAACTTGGTGAAGCAGGTTGTGGATCACCAAGAGAATCTTCATAAAAAGTATTTGGAAGTGATTGAAAAAATGGAGAAAGAGAGAAGGGAGAGAGAAGTGGCATGGAGATATCAAGCGGAGGAGAACCATAAGAGAGAAACCATGGCCAAACTCCATGAGCAGGCTCTAGCTTCAAGCAGAGAAGCTCTCATTGTTTCATACATTGAGGAAATCACTGGCCAAAGAGTCAGCCTTCCTCCTAGGCAAACCCCACTGTTGTTGCAGCCTGATAATGTAAGCGAACCCCAAACCGCAGTGGAATTGACACCCTCCCAAGCTGATCGCACAAATAGCAGATGGCCTCAAAGTGAAGTCGAGGCGTTGATTCTAGTGCGAGGTAGTGTAGAATCGAAATTTGTGGAACTGGGGTCAAAAGGGCATGTCTGGGAAGAGGTGAGTGCTTTAATGGCTTCAATGGGCTATCAGAAGAGTGCCAGAAGGTGCAAGCAAAAGTGGGAGAACATCAACAAGTACTTTAGGAGAACCAAAGACACTGCAAAAAAGCGTCCTATTCAGTCGAAAACTTGTTCTTATTTTAATCAGTTGGGTCAGCTGTACTCAAAAACGCCGACCACCGTCCCTTCATCTTCATCTTCGGTTAGCATGGATGTTAGCATTCAAAGGCAAGGTTATTCAGAGCTTTTAGAAGCCTTTGTAGCAGGGAGAGGGACAGAAGTTACACATAATCTGTCTAATGGGAATTTTGAAATCTCCGAAATGGGGTTCAGTAGGTTGGATTTCAATGGCATTAGTAATGGTAAAGTAGAAAATGTGCAAGGAGACCGTGGGAAGGAGAAGGAAAACGACGAGGATGACGAGAACATGGACGAAAGGGAAGACACGGATGGTGAAGATTTTGATGAATAA

Protein Analysis

562

Amino Acids

64.12

Weight (kDa)

5.86

Isoelectric Point (pI)

49.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_4 PF13837 371 - 457 9.4e-22 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015554)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g31050
malus_domestica MD08G1071000.v1.1 MD15G1050800.v1.1
prunus_persica Prupe.1G405000_v2.0.a1 Prupe.1G405000_v2.0.a1 Prupe.1G405000_v2.0.a1
pyrus_communis pycom08g05880
rosa_chinensis RchiOBHm_Chr6g0311751
rosa_laevigata RLG00000010377
rosa_multiflora Rmu_sc0002888.1_g000038
rosa_roxburghii Rroxscaffold_7G00157340
rosa_samantha Rh6AG506800 Rh6BG517400 Rh6CG523200 Rh6DG509000
rosa_wichuraiana Rw6G044080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 413
Acc36I ACCTGC 1 cut(s) 784
AccII CGCG 1 cut(s) 410
AciI CCGC 5 cut(s) 125, 134, 410, 896, 1071
AclWI GGATC 2 cut(s) 617, 810
AcoI YGGCCR 2 cut(s) 924, 997
AcsI RAATTY 4 cut(s) 10, 602, 1172, 1519
AfaI GTAC 2 cut(s) 1292, 1376
AfiI CCNNNNNNNGG 3 cut(s) 940, 1539, 1609
AjuI GAANNNNNNNTTGG 4 cut(s) 672, 704, 801, 833
Alw26I GTCTC 3 cut(s) 311, 462, 1593
AlwI GGATC 2 cut(s) 617, 810
AoxI GGCC 4 cut(s) 631, 924, 997, 1118
ApoI RAATTY 4 cut(s) 10, 602, 1172, 1519
AspA2I CCTAGG 1 cut(s) 1019
AspLEI GCGC 1 cut(s) 571
AspS9I GGNCC 1 cut(s) 323
AsuHPI GGTGA 6 cut(s) 509, 565, 797, 799, 1225, 1679
AsuII TTCGAA 1 cut(s) 8
AvaII GGWCC 1 cut(s) 323
AvrII CCTAGG 1 cut(s) 1019
BalI TGGCCA 2 cut(s) 926, 999
BbsI GAAGAC 2 cut(s) 67, 1662
BccI CCATC 3 cut(s) 523, 1110, 1658
BcoDI GTCTC 3 cut(s) 311, 462, 1593
BfaI CTAG 5 cut(s) 521, 768, 947, 1020, 1148
BfmI CTRYAG 2 cut(s) 21, 230
BfuAI ACCTGC 1 cut(s) 784
BlnI CCTAGG 1 cut(s) 1019
BmcAI AGTACT 1 cut(s) 1292
Bme18I GGWCC 1 cut(s) 323
BmgT120I GGNCC 1 cut(s) 323
BmrI ACTGGG 1 cut(s) 1193
BmsI GCATC 1 cut(s) 118
BmuI ACTGGG 1 cut(s) 1193
BoxI GACNNNNGTC 1 cut(s) 1394
BpiI GAAGAC 2 cut(s) 67, 1662
BplI GAGNNNNNCTC 2 cut(s) 951, 983
BpmI CTGGAG 1 cut(s) 630
Bpu14I TTCGAA 1 cut(s) 8
BsaI GGTCTC 2 cut(s) 311, 1593
BsaJI CCNNGG 4 cut(s) 272, 921, 1019, 1603
Bsc4I CCNNNNNNNGG 3 cut(s) 940, 1539, 1609
Bse118I RCCGGY 2 cut(s) 554, 575
Bse1I ACTGG 3 cut(s) 71, 1000, 1188
Bse3DI GCAATG 1 cut(s) 224
BseDI CCNNGG 4 cut(s) 272, 921, 1019, 1603
BseGI GGATG 5 cut(s) 121, 166, 1432, 1636, 1669
BseLI CCNNNNNNNGG 3 cut(s) 940, 1539, 1609
BseMI GCAATG 1 cut(s) 224
BseMII CTCAG 3 cut(s) 98, 166, 322
BseNI ACTGG 3 cut(s) 71, 1000, 1188
BseRI GAGGAG 2 cut(s) 710, 914
Bsh1236I CGCG 1 cut(s) 410
BshFI GGCC 4 cut(s) 633, 926, 999, 1120
BsiSI CCGG 2 cut(s) 555, 576
BslFI GGGAC 2 cut(s) 1382, 1501
BslI CCNNNNNNNGG 3 cut(s) 940, 1539, 1609
BsmAI GTCTC 3 cut(s) 311, 462, 1593
BsmFI GGGAC 2 cut(s) 1382, 1501
BsmI GAATGC 2 cut(s) 4, 1434
BsnI GGCC 4 cut(s) 633, 926, 999, 1120
Bso31I GGTCTC 2 cut(s) 311, 1593
Bsp119I TTCGAA 1 cut(s) 8
Bsp143I GATC 3 cut(s) 622, 802, 1099
Bsp19I CCATGG 2 cut(s) 272, 921
BspACI CCGC 5 cut(s) 125, 134, 410, 896, 1071
BspANI GGCC 4 cut(s) 633, 926, 999, 1120
BspCNI CTCAG 3 cut(s) 97, 165, 321
BspFNI CGCG 1 cut(s) 410
BspMAI CTGCAG 2 cut(s) 25, 234
BspMI ACCTGC 1 cut(s) 784
BspPI GGATC 2 cut(s) 617, 810
BspT104I TTCGAA 1 cut(s) 8
BspTNI GGTCTC 2 cut(s) 311, 1593
BsrDI GCAATG 1 cut(s) 224
BsrFI RCCGGY 2 cut(s) 554, 575
BsrI ACTGG 3 cut(s) 71, 1000, 1188
BssAI RCCGGY 2 cut(s) 554, 575
BssECI CCNNGG 4 cut(s) 272, 921, 1019, 1603
BssMI GATC 3 cut(s) 622, 802, 1099
BssT1I CCWWGG 3 cut(s) 272, 921, 1019
Bst4CI ACNGT 3 cut(s) 1035, 1396, 1604
Bst6I CTCTTC 5 cut(s) 373, 716, 722, 1203, 1241
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 3 cut(s) 266, 942, 1265
BstDEI CTNAG 4 cut(s) 84, 152, 308, 334
BstDSI CCRYGG 3 cut(s) 272, 921, 1603
BstF5I GGATG 5 cut(s) 121, 166, 1432, 1636, 1669
BstFNI CGCG 1 cut(s) 410
BstHHI GCGC 1 cut(s) 571
BstKTI GATC 3 cut(s) 625, 805, 1102
BstMAI GTCTC 3 cut(s) 311, 462, 1593
BstMBI GATC 3 cut(s) 622, 802, 1099
BstNSI RCATGY 1 cut(s) 1202
BstPAI GACNNNNGTC 1 cut(s) 1394
BstSFI CTRYAG 2 cut(s) 21, 230
BstUI CGCG 1 cut(s) 410
BstV2I GAAGAC 2 cut(s) 67, 1662
BsuRI GGCC 4 cut(s) 633, 926, 999, 1120
BtgI CCRYGG 3 cut(s) 272, 921, 1603
BtsCI GGATG 5 cut(s) 121, 166, 1432, 1636, 1669
BtsI GCAGTG 3 cut(s) 325, 1080, 1311
BtsIMutI CAGTG 7 cut(s) 316, 325, 711, 993, 1031, 1080, 1311
BveI ACCTGC 1 cut(s) 784
Cac8I GCNNGC 3 cut(s) 266, 942, 1265
CfoI GCGC 1 cut(s) 571
Cfr10I RCCGGY 2 cut(s) 554, 575
Cfr13I GGNCC 1 cut(s) 323
CseI GACGC 3 cut(s) 416, 672, 1313
Csp6I GTAC 2 cut(s) 1291, 1375
CspCI CAANNNNNGTGG 2 cut(s) 1020, 1055
CviAII CATG 8 cut(s) 141, 273, 882, 922, 935, 1199, 1423, 1642
CviQI GTAC 2 cut(s) 1291, 1375
DdeI CTNAG 4 cut(s) 84, 152, 308, 334
DpnI GATC 3 cut(s) 624, 804, 1101
DpnII GATC 3 cut(s) 622, 802, 1099
DrdI GACNNNNNNGTC 1 cut(s) 413
DseDI GACNNNNNNGTC 1 cut(s) 413
EaeI YGGCCR 2 cut(s) 924, 997
Eam1104I CTCTTC 5 cut(s) 373, 716, 722, 1203, 1241
EarI CTCTTC 5 cut(s) 373, 716, 722, 1203, 1241
Eco130I CCWWGG 3 cut(s) 272, 921, 1019
Eco31I GGTCTC 2 cut(s) 311, 1593
Eco32I GATATC 1 cut(s) 890
Eco47I GGWCC 1 cut(s) 323
EcoRV GATATC 1 cut(s) 890
EcoT14I CCWWGG 3 cut(s) 272, 921, 1019
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 3 cut(s) 272, 921, 1019
FaeI CATG 8 cut(s) 144, 276, 885, 925, 938, 1202, 1426, 1645
FalI AAGNNNNNCTT 2 cut(s) 801, 833
FaqI GGGAC 2 cut(s) 1382, 1501
FatI CATG 8 cut(s) 140, 272, 881, 921, 934, 1198, 1422, 1641
FokI GGATG 5 cut(s) 108, 153, 1439, 1643, 1676
FspBI CTAG 5 cut(s) 521, 768, 947, 1020, 1148
GlaI GCGC 1 cut(s) 570
GsuI CTGGAG 1 cut(s) 630
HaeIII GGCC 4 cut(s) 633, 926, 999, 1120
HapII CCGG 2 cut(s) 555, 576
HgaI GACGC 3 cut(s) 416, 672, 1313
HhaI GCGC 1 cut(s) 571
Hin1II CATG 8 cut(s) 144, 276, 885, 925, 938, 1202, 1426, 1645
Hin6I GCGC 1 cut(s) 569
HinP1I GCGC 1 cut(s) 569
HindIII AAGCTT 1 cut(s) 299
HpaII CCGG 2 cut(s) 555, 576
HphI GGTGA 6 cut(s) 509, 565, 797, 799, 1225, 1679
Hpy166II GTNNAC 1 cut(s) 323
Hpy188I TCNGA 9 cut(s) 87, 171, 177, 546, 679, 720, 1245, 1456, 1534
Hpy188III TCNNGA 2 cut(s) 404, 778
Hpy8I GTNNAC 1 cut(s) 323
Hpy99I CGWCG 1 cut(s) 1628
HpyCH4III ACNGT 3 cut(s) 1035, 1396, 1604
HpyCH4V TGCA 9 cut(s) 4, 23, 109, 232, 465, 1042, 1263, 1316, 1594
HpyF3I CTNAG 4 cut(s) 84, 152, 308, 334
Hsp92II CATG 8 cut(s) 144, 276, 885, 925, 938, 1202, 1426, 1645
HspAI GCGC 1 cut(s) 569
Kzo9I GATC 3 cut(s) 622, 802, 1099
LmnI GCTCC 5 cut(s) 17, 216, 649, 697, 748
LweI GCATC 1 cut(s) 118
MaeI CTAG 5 cut(s) 521, 768, 947, 1020, 1148
MaeIII GTNAC 1 cut(s) 1495
MalI GATC 3 cut(s) 624, 804, 1101
MboI GATC 3 cut(s) 622, 802, 1099
MlsI TGGCCA 2 cut(s) 926, 999
MluNI TGGCCA 2 cut(s) 926, 999
MlyI GAGTC 1 cut(s) 1014
MmeI TCCRAC 4 cut(s) 220, 374, 657, 1533
Mox20I TGGCCA 2 cut(s) 926, 999
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 2 cut(s) 926, 999
MseI TTAA 3 cut(s) 755, 1223, 1356
Msp20I TGGCCA 2 cut(s) 926, 999
MspA1I CMGCKG 2 cut(s) 650, 1372
MspI CCGG 2 cut(s) 555, 576
Mva1269I GAATGC 2 cut(s) 4, 1434
MvnI CGCG 1 cut(s) 410
NcoI CCATGG 2 cut(s) 272, 921
NdeII GATC 3 cut(s) 622, 802, 1099
NlaIII CATG 8 cut(s) 144, 276, 885, 925, 938, 1202, 1426, 1645
NmeAIII GCCGAG 1 cut(s) 306
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 1202
NspV TTCGAA 1 cut(s) 8
PcsI WCGNNNNNNNCGW 1 cut(s) 1632
PctI GAATGC 2 cut(s) 4, 1434
PleI GAGTC 1 cut(s) 1013
PpsI GAGTC 1 cut(s) 1013
PshAI GACNNNNGTC 1 cut(s) 1394
PspPI GGNCC 1 cut(s) 323
PstI CTGCAG 2 cut(s) 25, 234
PvuII CAGCTG 2 cut(s) 650, 1372
RsaI GTAC 2 cut(s) 1292, 1376
RsaNI GTAC 2 cut(s) 1291, 1375
SaqAI TTAA 3 cut(s) 755, 1223, 1356
Sau3AI GATC 3 cut(s) 622, 802, 1099
Sau96I GGNCC 1 cut(s) 323
ScaI AGTACT 1 cut(s) 1292
SchI GAGTC 1 cut(s) 1014
SfaNI GCATC 1 cut(s) 118
SfcI CTRYAG 2 cut(s) 21, 230
SfuI TTCGAA 1 cut(s) 8
SgrAI CRCCGGYG 1 cut(s) 575
SinI GGWCC 1 cut(s) 323
SsiI CCGC 5 cut(s) 125, 134, 410, 896, 1071
SspI AATATT 1 cut(s) 365
SspMI CTAG 5 cut(s) 521, 768, 947, 1020, 1148
StyI CCWWGG 3 cut(s) 272, 921, 1019
TaaI ACNGT 3 cut(s) 1035, 1396, 1604
TaqI TCGA 7 cut(s) 8, 590, 737, 777, 1134, 1169, 1337
TatI WGTACW 2 cut(s) 1290, 1374
TauI GCSGC 1 cut(s) 136
Tru1I TTAA 3 cut(s) 755, 1223, 1356
Tru9I TTAA 3 cut(s) 755, 1223, 1356
TscAI CASTG 7 cut(s) 316, 325, 711, 1000, 1038, 1080, 1318
TspDTI ATGAA 7 cut(s) 48, 110, 758, 809, 966, 1392, 1398
TspGWI ACGGA 1 cut(s) 1676
TspRI CASTG 7 cut(s) 316, 325, 711, 1000, 1038, 1080, 1318
VpaK11BI GGWCC 1 cut(s) 323
XapI RAATTY 4 cut(s) 10, 602, 1172, 1519
XceI RCATGY 1 cut(s) 1202
XmaJI CCTAGG 1 cut(s) 1019
XspI CTAG 5 cut(s) 521, 768, 947, 1020, 1148
ZrmI AGTACT 1 cut(s) 1292
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.