MD08G1071000.v1.1
MYB Family

Trihelix transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015554
Physical Location & Seq
Reverse (-)
5623763 .. 5626102
2340 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1071000.v1.1.491

Sequence Viewer

Length: 1689 bp
ATGCAGTCGAATTTTGAAACAGCAGAGATTGGTAACCAACAATTCATGGATTCTAGTTCATCAGTTTTACCAATCTTCAATCCTCAGAAGCAAAACCAGAACCATTTCCTTCAACAGTTTCATCAGCAGCAGCCGTTTAGTCATCTCAGTCAAATAGCTCAACCGATTCCGATTACCCACGACCTGTTTCAACGACAGAATCATCTCCAGTTCCAAGCATTGCTGCAGCAGCAGCAGGAAGAACAGCAGGAAGAAAGCAGGCTGCCATGGCAGCCGAGCCCTCTAAGTTTCAAGCTTGGTCTCAGTGAAAACACTGGACCACATAACTTAGGATTCAAAAATTGGCAAACCCAAGAATATTATGGGATTATAAGAGAACCCATGTGTTGGAAGCCTCTTGACACGGTAGTCCCCGACACAACAAACAACAGGCAGAGGCAGGAAGAAGGGGAGAGATGCAGAGACTTGGAGGAAAAACACAGGCTTTATGGTGAGCTTGAAGCAATCTATAACCTTGCAAAGATGGGGGAGGGTAATCAGAATCAAACTGGCTCTGGCTCTGCTCTCACCGGCGAAAACTGCCCGAAAAATGTGGGATTTCCTGTGGTTTTTGGTGATTCTAATGGCCTCAATGCTGCTCCAGCTGATACATTTAGGGTGGATAACAGATCGGAAGACTCCATTGGAGAAGAGGTGTCGGTGACGAAGATTCAGAAGAGGAAGAGGAAGAGGAATATGAAGGAGCAATTAAGTTCAATGACTAGGTTTTTTGAGAACTTGGTGAAGCAGGTTGTGGATCACCAAGAGAATCTTCATAAGAAGTATTTGGAAGTGATTGAAAAAATGGAAAAAGAGAGAAGGGAGAGAGAAGAAGCATGGAGATGTCAAGCGGAGGAGAACCATAAGAGAGAAGCCATTGCCAAACTCCATGAGCAGGCTATAGCTTCAAGCAGAGAAGCTCTCATTGTTTCATACATTGAGAAAATCACTGGCCAAAGAGTCAACCTTCCTCCTAGGAAAACCCCATTGTTGTTGCAGCCCGATAATTTAAGCGAACCTGAAACCGCAGAGGAATTGACACCCTCCAAAGCTGAACACACAAATAGCAGATGGCCTCAAAATGAAGTCGAGGCTTTGATTCTAGTGCGCAGTAGTGTAGAGTCGAAATTTGCGGAACAGGGGTCAAAAGGGCATGTTTGGGAAGAGGTGAGTGCTTTAATGGCTTCAATGGGCTATCAGAAAAGTGCCAGAAGGTGCAAGCAAAAGTGGGAGAACATCAACAAGTACTTTAGGAAAACCAAAGACGCTGCGAAAAAGCGTCCTCTTCAGTCGAAAACTTGTTCTTATTTTAGTCAATTGGGTCAGCTGTACTCAAAAACACCGACCGTCCCGTCATCTTCGTCTTCGGTTAGCATGGATGTTAGCATTCAAAGGCAAGGTTATTCGGAGCTTTTAGAAGCCTTTATTGCAGGAAGAGAGACGGGAGTTACACATAATCTGCCAAATGGGAATTTTGAAAGCTCCGAAATGGGTTTGAGTAGGTTGGATTTCAATGGCATTGGTGATGGTAAAGTAGAACATATGCAAGGAGAATCTAGGAAGGAGAAGGAAAACGATGAGGATGACGAAAACATGGATGAAAGAGAAGACACGGATGGTGATGATTCTGATGAATACGGTAGAGCATAG

Protein Analysis

563

Amino Acids

64.49

Weight (kDa)

5.57

Isoelectric Point (pI)

52.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_4 PF13837 369 - 455 5.6e-22 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015554)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g31050
malus_domestica MD08G1071000.v1.1 MD15G1050800.v1.1
prunus_persica Prupe.1G405000_v2.0.a1 Prupe.1G405000_v2.0.a1 Prupe.1G405000_v2.0.a1
pyrus_communis pycom08g05880
rosa_chinensis RchiOBHm_Chr6g0311751
rosa_laevigata RLG00000010377
rosa_multiflora Rmu_sc0002888.1_g000038
rosa_roxburghii Rroxscaffold_7G00157340
rosa_samantha Rh6AG506800 Rh6BG517400 Rh6CG523200 Rh6DG509000
rosa_wichuraiana Rw6G044080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 371
AasI GACNNNNNNGTC 2 cut(s) 407, 1390
Acc16I TGCGCA 1 cut(s) 1148
Acc36I ACCTGC 1 cut(s) 778
AccB7I CCANNNNNTGG 1 cut(s) 387
AciI CCGC 3 cut(s) 890, 1065, 1172
AclWI GGATC 1 cut(s) 804
AcoI YGGCCR 1 cut(s) 991
AcsI RAATTY 3 cut(s) 10, 1166, 1510
AcuI CTGAAG 1 cut(s) 1310
AfaI GTAC 2 cut(s) 1286, 1370
AfiI CCNNNNNNNGG 2 cut(s) 387, 934
AjuI GAANNNNNNNTTGG 4 cut(s) 666, 698, 795, 827
Alw26I GTCTC 3 cut(s) 305, 456, 1472
AlwI GGATC 1 cut(s) 804
AoxI GGCC 3 cut(s) 625, 991, 1112
ApoI RAATTY 3 cut(s) 10, 1166, 1510
Asp700I GAANNNNTTC 1 cut(s) 104
AspA2I CCTAGG 1 cut(s) 1013
AspLEI GCGC 1 cut(s) 1149
AspS9I GGNCC 1 cut(s) 317
AsuHPI GGTGA 9 cut(s) 503, 559, 626, 712, 791, 793, 1219, 1574, 1670
AvaII GGWCC 1 cut(s) 317
AvrII CCTAGG 1 cut(s) 1013
BalI TGGCCA 1 cut(s) 993
BanII GRGCYC 1 cut(s) 281
BbsI GAAGAC 3 cut(s) 681, 1395, 1653
BccI CCATC 4 cut(s) 517, 1104, 1559, 1649
BceAI ACGGC 1 cut(s) 118
BcoDI GTCTC 3 cut(s) 305, 456, 1472
BfaI CTAG 5 cut(s) 54, 762, 1014, 1142, 1596
BfmI CTRYAG 2 cut(s) 224, 939
BfuAI ACCTGC 1 cut(s) 778
BlnI CCTAGG 1 cut(s) 1013
BmcAI AGTACT 1 cut(s) 1286
Bme18I GGWCC 1 cut(s) 317
BmgT120I GGNCC 1 cut(s) 317
BmsI GCATC 1 cut(s) 446
BpiI GAAGAC 3 cut(s) 681, 1395, 1653
BplI GAGNNNNNCTC 2 cut(s) 945, 977
BpmI CTGGAG 2 cut(s) 191, 624
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 2 cut(s) 266, 1013
BsaXI ACNNNNNCTCC 2 cut(s) 678, 708
Bsc4I CCNNNNNNNGG 2 cut(s) 387, 934
Bse118I RCCGGY 1 cut(s) 569
Bse1I ACTGG 4 cut(s) 208, 319, 553, 994
Bse3DI GCAATG 2 cut(s) 218, 915
BseDI CCNNGG 2 cut(s) 266, 1013
BseGI GGATG 4 cut(s) 1423, 1627, 1642, 1660
BseLI CCNNNNNNNGG 2 cut(s) 387, 934
BseMI GCAATG 2 cut(s) 218, 915
BseMII CTCAG 3 cut(s) 98, 160, 316
BseNI ACTGG 4 cut(s) 208, 319, 553, 994
BseRI GAGGAG 1 cut(s) 908
Bsh1285I CGRYCG 1 cut(s) 1386
BshFI GGCC 3 cut(s) 627, 993, 1114
BsiEI CGRYCG 1 cut(s) 1386
BsiSI CCGG 1 cut(s) 570
BslFI GGGAC 2 cut(s) 395, 1373
BslI CCNNNNNNNGG 2 cut(s) 387, 934
BsmAI GTCTC 3 cut(s) 305, 456, 1472
BsmBI CGTCTC 1 cut(s) 1472
BsmFI GGGAC 2 cut(s) 395, 1373
BsmI GAATGC 1 cut(s) 1425
BsnI GGCC 3 cut(s) 627, 993, 1114
Bso31I GGTCTC 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 281
Bsp143I GATC 2 cut(s) 668, 796
Bsp19I CCATGG 1 cut(s) 266
BspACI CCGC 3 cut(s) 890, 1065, 1172
BspANI GGCC 3 cut(s) 627, 993, 1114
BspCNI CTCAG 3 cut(s) 97, 159, 315
BspMAI CTGCAG 1 cut(s) 228
BspMI ACCTGC 1 cut(s) 778
BspPI GGATC 1 cut(s) 804
BspTNI GGTCTC 1 cut(s) 305
BsrDI GCAATG 2 cut(s) 218, 915
BsrFI RCCGGY 1 cut(s) 569
BsrI ACTGG 4 cut(s) 208, 319, 553, 994
BssAI RCCGGY 1 cut(s) 569
BssECI CCNNGG 2 cut(s) 266, 1013
BssMI GATC 2 cut(s) 668, 796
BssT1I CCWWGG 2 cut(s) 266, 1013
Bst4CI ACNGT 4 cut(s) 117, 406, 1387, 1679
Bst6I CTCTTC 7 cut(s) 684, 710, 716, 722, 1197, 1329, 1468
BstC8I GCNNGC 3 cut(s) 260, 936, 1259
BstDEI CTNAG 5 cut(s) 84, 146, 284, 302, 328
BstDSI CCRYGG 1 cut(s) 266
BstEII GGTNACC 1 cut(s) 32
BstF5I GGATG 4 cut(s) 1423, 1627, 1642, 1660
BstHHI GCGC 1 cut(s) 1149
BstKTI GATC 2 cut(s) 671, 799
BstMAI GTCTC 3 cut(s) 305, 456, 1472
BstMBI GATC 2 cut(s) 668, 796
BstMCI CGRYCG 1 cut(s) 1386
BstMWI GCNNNNNNNGC 8 cut(s) 229, 232, 268, 271, 579, 641, 1220, 1466
BstNSI RCATGY 1 cut(s) 1196
BstPI GGTNACC 1 cut(s) 32
BstSFI CTRYAG 2 cut(s) 224, 939
BstV2I GAAGAC 3 cut(s) 681, 1395, 1653
BsuRI GGCC 3 cut(s) 627, 993, 1114
BtgI CCRYGG 1 cut(s) 266
BtsCI GGATG 4 cut(s) 1423, 1627, 1642, 1660
BtsIMutI CAGTG 3 cut(s) 310, 312, 987
BveI ACCTGC 1 cut(s) 778
Cac8I GCNNGC 3 cut(s) 260, 936, 1259
CfoI GCGC 1 cut(s) 1149
Cfr10I RCCGGY 1 cut(s) 569
Cfr13I GGNCC 1 cut(s) 317
CseI GACGC 2 cut(s) 1307, 1313
Csp6I GTAC 2 cut(s) 1285, 1369
CviAII CATG 8 cut(s) 46, 267, 382, 876, 929, 1193, 1414, 1633
CviQI GTAC 2 cut(s) 1285, 1369
DdeI CTNAG 5 cut(s) 84, 146, 284, 302, 328
DpnI GATC 2 cut(s) 670, 798
DpnII GATC 2 cut(s) 668, 796
DrdI GACNNNNNNGTC 2 cut(s) 407, 1390
DseDI GACNNNNNNGTC 2 cut(s) 407, 1390
EaeI YGGCCR 1 cut(s) 991
Eam1104I CTCTTC 7 cut(s) 684, 710, 716, 722, 1197, 1329, 1468
EarI CTCTTC 7 cut(s) 684, 710, 716, 722, 1197, 1329, 1468
Eco130I CCWWGG 2 cut(s) 266, 1013
Eco24I GRGCYC 1 cut(s) 281
Eco31I GGTCTC 1 cut(s) 305
Eco47I GGWCC 1 cut(s) 317
Eco57I CTGAAG 1 cut(s) 1310
Eco91I GGTNACC 1 cut(s) 32
EcoO65I GGTNACC 1 cut(s) 32
EcoT14I CCWWGG 2 cut(s) 266, 1013
EcoT38I GRGCYC 1 cut(s) 281
ErhI CCWWGG 2 cut(s) 266, 1013
Esp3I CGTCTC 1 cut(s) 1472
FaeI CATG 8 cut(s) 49, 270, 385, 879, 932, 1196, 1417, 1636
FalI AAGNNNNNCTT 2 cut(s) 795, 827
FaqI GGGAC 2 cut(s) 395, 1373
FatI CATG 8 cut(s) 45, 266, 381, 875, 928, 1192, 1413, 1632
FauNDI CATATG 1 cut(s) 1581
FokI GGATG 4 cut(s) 1430, 1634, 1649, 1667
FriOI GRGCYC 1 cut(s) 281
FspBI CTAG 5 cut(s) 54, 762, 1014, 1142, 1596
FspI TGCGCA 1 cut(s) 1148
GlaI GCGC 1 cut(s) 1148
GsuI CTGGAG 2 cut(s) 191, 624
HaeIII GGCC 3 cut(s) 627, 993, 1114
HapII CCGG 1 cut(s) 570
HgaI GACGC 2 cut(s) 1307, 1313
HhaI GCGC 1 cut(s) 1149
Hin1II CATG 8 cut(s) 49, 270, 385, 879, 932, 1196, 1417, 1636
Hin6I GCGC 1 cut(s) 1147
HinP1I GCGC 1 cut(s) 1147
HincII GTYRAC 1 cut(s) 1003
HindII GTYRAC 1 cut(s) 1003
HindIII AAGCTT 1 cut(s) 293
HpaII CCGG 1 cut(s) 570
HphI GGTGA 9 cut(s) 503, 559, 626, 712, 791, 793, 1219, 1574, 1670
Hpy166II GTNNAC 1 cut(s) 1003
Hpy188I TCNGA 9 cut(s) 87, 171, 540, 673, 714, 1239, 1447, 1525, 1669
Hpy188III TCNNGA 1 cut(s) 398
Hpy8I GTNNAC 1 cut(s) 1003
HpyAV CCTTC 8 cut(s) 119, 440, 733, 852, 1016, 1245, 1594, 1600
HpyCH4III ACNGT 4 cut(s) 117, 406, 1387, 1679
HpyCH4V TGCA 8 cut(s) 4, 226, 459, 518, 1036, 1257, 1469, 1585
HpyF10VI GCNNNNNNNGC 8 cut(s) 229, 232, 268, 271, 579, 641, 1220, 1466
HpyF3I CTNAG 5 cut(s) 84, 146, 284, 302, 328
Hsp92II CATG 8 cut(s) 49, 270, 385, 879, 932, 1196, 1417, 1636
HspAI GCGC 1 cut(s) 1147
Kzo9I GATC 2 cut(s) 668, 796
LmnI GCTCC 4 cut(s) 643, 742, 1447, 1526
LweI GCATC 1 cut(s) 446
MaeI CTAG 5 cut(s) 54, 762, 1014, 1142, 1596
MaeIII GTNAC 3 cut(s) 32, 700, 1486
MalI GATC 2 cut(s) 670, 798
MboI GATC 2 cut(s) 668, 796
MfeI CAATTG 1 cut(s) 1355
MhlI GDGCHC 1 cut(s) 281
MlsI TGGCCA 1 cut(s) 993
MluCI AATT 9 cut(s) 10, 41, 340, 746, 1045, 1073, 1166, 1355, 1510
MluNI TGGCCA 1 cut(s) 993
MlyI GAGTC 3 cut(s) 671, 1008, 1169
MmeI TCCRAC 2 cut(s) 368, 1524
Mox20I TGGCCA 1 cut(s) 993
MroXI GAANNNNTTC 1 cut(s) 104
MscI TGGCCA 1 cut(s) 993
MseI TTAA 3 cut(s) 749, 1049, 1217
MslI CAYNNNNRTG 1 cut(s) 880
Msp20I TGGCCA 1 cut(s) 993
MspA1I CMGCKG 2 cut(s) 644, 1366
MspI CCGG 1 cut(s) 570
MunI CAATTG 1 cut(s) 1355
Mva1269I GAATGC 1 cut(s) 1425
MwoI GCNNNNNNNGC 8 cut(s) 229, 232, 268, 271, 579, 641, 1220, 1466
NcoI CCATGG 1 cut(s) 266
NdeI CATATG 1 cut(s) 1581
NdeII GATC 2 cut(s) 668, 796
NlaIII CATG 8 cut(s) 49, 270, 385, 879, 932, 1196, 1417, 1636
NmeAIII GCCGAG 1 cut(s) 300
NmuCI GTSAC 1 cut(s) 700
NsbI TGCGCA 1 cut(s) 1148
NspI RCATGY 1 cut(s) 1196
PctI GAATGC 1 cut(s) 1425
PdmI GAANNNNTTC 1 cut(s) 104
PflMI CCANNNNNTGG 1 cut(s) 387
PleI GAGTC 3 cut(s) 671, 1007, 1168
PpsI GAGTC 3 cut(s) 671, 1007, 1168
PsiI TTATAA 1 cut(s) 371
PspEI GGTNACC 1 cut(s) 32
PspPI GGNCC 1 cut(s) 317
PstI CTGCAG 1 cut(s) 228
PvuII CAGCTG 2 cut(s) 644, 1366
RsaI GTAC 2 cut(s) 1286, 1370
RsaNI GTAC 2 cut(s) 1285, 1369
RseI CAYNNNNRTG 1 cut(s) 880
SaqAI TTAA 3 cut(s) 749, 1049, 1217
Sau3AI GATC 2 cut(s) 668, 796
Sau96I GGNCC 1 cut(s) 317
ScaI AGTACT 1 cut(s) 1286
SchI GAGTC 3 cut(s) 671, 1008, 1169
SduI GDGCHC 1 cut(s) 281
SfaNI GCATC 1 cut(s) 446
SfcI CTRYAG 2 cut(s) 224, 939
SgrAI CRCCGGYG 1 cut(s) 569
SinI GGWCC 1 cut(s) 317
SmiMI CAYNNNNRTG 1 cut(s) 880
Sse9I AATT 9 cut(s) 10, 41, 340, 746, 1045, 1073, 1166, 1355, 1510
SsiI CCGC 3 cut(s) 890, 1065, 1172
SspI AATATT 1 cut(s) 359
SspMI CTAG 5 cut(s) 54, 762, 1014, 1142, 1596
StyI CCWWGG 2 cut(s) 266, 1013
TaaI ACNGT 4 cut(s) 117, 406, 1387, 1679
TaqI TCGA 4 cut(s) 8, 1128, 1163, 1331
TasI AATT 9 cut(s) 10, 41, 340, 746, 1045, 1073, 1166, 1355, 1510
TatI WGTACW 2 cut(s) 1284, 1368
Tru1I TTAA 3 cut(s) 749, 1049, 1217
Tru9I TTAA 3 cut(s) 749, 1049, 1217
TscAI CASTG 3 cut(s) 310, 319, 994
TseFI GTSAC 1 cut(s) 700
Tsp45I GTSAC 1 cut(s) 700
TspDTI ATGAA 9 cut(s) 34, 48, 110, 752, 803, 960, 1137, 1653, 1686
TspGWI ACGGA 1 cut(s) 1667
TspRI CASTG 3 cut(s) 310, 319, 994
Van91I CCANNNNNTGG 1 cut(s) 387
VpaK11BI GGWCC 1 cut(s) 317
XapI RAATTY 3 cut(s) 10, 1166, 1510
XceI RCATGY 1 cut(s) 1196
XcmI CCANNNNNNNNNTGG 1 cut(s) 359
XmaJI CCTAGG 1 cut(s) 1013
XmnI GAANNNNTTC 1 cut(s) 104
XspI CTAG 5 cut(s) 54, 762, 1014, 1142, 1596
ZrmI AGTACT 1 cut(s) 1286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.