RLG00000028783

RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0022881
Physical Location & Seq
Forward (+)
27912317 .. 27920135
7819 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028783

Sequence Viewer

Length: 525 bp
ATGAGTAGAAACTACAGCCACTCGAAGGAAGAGGATTGCACATGGGGGAGCTGGGAGAAGGGGGTTGATCTGGAAGGAGTTAGGTTGGAAGACAGAAAAATAATGGAGGAAGATCGTAGATTGAAGGTTTTCAAGGAGGAGTGGTTTGAAGGCAAGGACTGTCTTGACATTGGCTGCTATTCTGGGGTCAACACTATACAAATCGTTGATTTGGTTTCCGCCGAAACCCTATTCAATTCCTCACAGTTCGCCCCCACAGTTTTTACGCTCAGAGAAGTGACCGGCGCCAAAACCACGTCTACTCCTCAATTCGCTGCTCACAGGCTCAAGTTCTCAAGAAGCTCCAGTGAGAGATTCGCCTCCAAAGAACTAGTTTCCTCAAGAGGTATCAACCAAAGGCAAACTTTTTGTATAATTTATAAAGTTGAGCCTTACCACTGCTTTGGAATGACCATCCTGATGTCCTGGGAGGATTTTAATTCCAGGAAACAGGCAATTTCAGAAGCAGAAGACATTGGGCTCTGA

Protein Analysis

175

Amino Acids

20.03

Weight (kDa)

5.5

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022881)

Species Orthologous Gene IDs
rosa_laevigata RLG00000009150 RLG00000028783
rosa_samantha Rh5CG528100 Rh7CG136900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 420
AccB1I GGYRCC 1 cut(s) 284
AccI GTMKAC 1 cut(s) 299
AciI CCGC 1 cut(s) 219
AcyI GRCGYC 1 cut(s) 285
AfiI CCNNNNNNNGG 1 cut(s) 25
AgsI TTSAA 4 cut(s) 124, 133, 149, 235
AhlI ACTAGT 1 cut(s) 370
AjiI CACGTC 1 cut(s) 297
AjnI CCWGG 2 cut(s) 464, 482
AluBI AGCT 2 cut(s) 51, 342
AluI AGCT 2 cut(s) 51, 342
ApeKI GCWGC 2 cut(s) 174, 314
Asp700I GAANNNNTTC 1 cut(s) 128
AspLEI GCGC 1 cut(s) 287
BanI GGYRCC 1 cut(s) 284
BanII GRGCYC 1 cut(s) 522
BbsI GAAGAC 2 cut(s) 96, 516
BbvI GCAGC 2 cut(s) 161, 301
BccI CCATC 1 cut(s) 461
BciT130I CCWGG 2 cut(s) 466, 484
BcuI ACTAGT 1 cut(s) 370
BfaI CTAG 1 cut(s) 371
BfmI CTRYAG 1 cut(s) 13
BfoI RGCGCY 1 cut(s) 288
BisI GCNGC 2 cut(s) 175, 315
BlsI GCNGC 2 cut(s) 176, 316
Bme1390I CCNGG 2 cut(s) 466, 484
BmgBI CACGTC 1 cut(s) 297
BmiI GGNNCC 1 cut(s) 286
BmrFI CCNGG 2 cut(s) 466, 484
BpiI GAAGAC 2 cut(s) 96, 516
BpmI CTGGAG 1 cut(s) 328
BpuEI CTTGAG 3 cut(s) 311, 319, 364
BsaHI GRCGYC 1 cut(s) 285
BsaJI CCNNGG 1 cut(s) 465
BsaXI ACNNNNNCTCC 4 cut(s) 128, 158, 286, 316
Bsc4I CCNNNNNNNGG 1 cut(s) 25
Bse118I RCCGGY 1 cut(s) 281
Bse1I ACTGG 1 cut(s) 345
BseBI CCWGG 2 cut(s) 466, 484
BseDI CCNNGG 1 cut(s) 465
BseGI GGATG 1 cut(s) 453
BseLI CCNNNNNNNGG 1 cut(s) 25
BseMII CTCAG 1 cut(s) 283
BseNI ACTGG 1 cut(s) 345
BseRI GAGGAG 2 cut(s) 152, 294
BseXI GCAGC 2 cut(s) 161, 301
BseYI CCCAGC 1 cut(s) 51
BshNI GGYRCC 1 cut(s) 284
BsiSI CCGG 1 cut(s) 282
BslI CCNNNNNNNGG 1 cut(s) 25
Bsp1286I GDGCHC 1 cut(s) 522
Bsp143I GATC 2 cut(s) 67, 112
BspACI CCGC 1 cut(s) 219
BspCNI CTCAG 1 cut(s) 282
BspLI GGNNCC 1 cut(s) 286
BspT107I GGYRCC 1 cut(s) 284
BsrFI RCCGGY 1 cut(s) 281
BsrI ACTGG 1 cut(s) 345
BssAI RCCGGY 1 cut(s) 281
BssECI CCNNGG 1 cut(s) 465
BssMI GATC 2 cut(s) 67, 112
BssNI GRCGYC 1 cut(s) 285
Bst2UI CCWGG 2 cut(s) 466, 484
Bst4CI ACNGT 3 cut(s) 161, 246, 259
Bst6I CTCTTC 1 cut(s) 24
BstACI GRCGYC 1 cut(s) 285
BstDEI CTNAG 1 cut(s) 269
BstF5I GGATG 1 cut(s) 453
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 1 cut(s) 287
BstKTI GATC 2 cut(s) 70, 115
BstMBI GATC 2 cut(s) 67, 112
BstNI CCWGG 2 cut(s) 466, 484
BstSCI CCNGG 2 cut(s) 464, 482
BstSFI CTRYAG 1 cut(s) 13
BstV1I GCAGC 2 cut(s) 161, 301
BstV2I GAAGAC 2 cut(s) 96, 516
BstXI CCANNNNNNTGG 1 cut(s) 443
BtrI CACGTC 1 cut(s) 297
BtsCI GGATG 1 cut(s) 453
BtsI GCAGTG 1 cut(s) 436
BtsIMutI CAGTG 2 cut(s) 352, 436
CfoI GCGC 1 cut(s) 287
Cfr10I RCCGGY 1 cut(s) 281
CviAII CATG 1 cut(s) 42
CviJI RGCY 7 cut(s) 18, 51, 174, 325, 342, 430, 520
CviKI_1 RGCY 7 cut(s) 18, 51, 174, 325, 342, 430, 520
DdeI CTNAG 1 cut(s) 269
DinI GGCGCC 1 cut(s) 286
DpnI GATC 2 cut(s) 69, 114
DpnII GATC 2 cut(s) 67, 112
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
EciI GGCGGA 1 cut(s) 208
Eco24I GRGCYC 1 cut(s) 522
EcoRII CCWGG 2 cut(s) 464, 482
EcoT38I GRGCYC 1 cut(s) 522
EgeI GGCGCC 1 cut(s) 286
EheI GGCGCC 1 cut(s) 286
FaeI CATG 1 cut(s) 45
FaiI YATR 4 cut(s) 43, 197, 413, 420
FalI AAGNNNNNCTT 2 cut(s) 388, 420
FatI CATG 1 cut(s) 41
FblI GTMKAC 1 cut(s) 299
Fnu4HI GCNGC 2 cut(s) 175, 315
FokI GGATG 1 cut(s) 440
FriOI GRGCYC 1 cut(s) 522
Fsp4HI GCNGC 2 cut(s) 175, 315
FspBI CTAG 1 cut(s) 371
GlaI GCGC 1 cut(s) 286
GluI GCNGC 2 cut(s) 175, 315
GsaI CCCAGC 1 cut(s) 55
GsuI CTGGAG 1 cut(s) 328
HaeII RGCGCY 1 cut(s) 288
HapII CCGG 1 cut(s) 282
HhaI GCGC 1 cut(s) 287
Hin1I GRCGYC 1 cut(s) 285
Hin1II CATG 1 cut(s) 45
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HinfI GANTC 1 cut(s) 354
HpaII CCGG 1 cut(s) 282
Hpy166II GTNNAC 2 cut(s) 190, 300
Hpy188I TCNGA 3 cut(s) 272, 502, 524
Hpy188III TCNNGA 5 cut(s) 71, 164, 336, 381, 457
Hpy8I GTNNAC 2 cut(s) 190, 300
HpyAV CCTTC 5 cut(s) 19, 52, 68, 118, 143
HpyCH4III ACNGT 3 cut(s) 161, 246, 259
HpyCH4IV ACGT 1 cut(s) 296
HpyCH4V TGCA 1 cut(s) 39
HpyF3I CTNAG 1 cut(s) 269
HpySE526I ACGT 1 cut(s) 296
Hsp92I GRCGYC 1 cut(s) 285
Hsp92II CATG 1 cut(s) 45
HspAI GCGC 1 cut(s) 285
KasI GGCGCC 1 cut(s) 284
Kzo9I GATC 2 cut(s) 67, 112
LmnI GCTCC 2 cut(s) 48, 347
Lsp1109I GCAGC 2 cut(s) 161, 301
MaeI CTAG 1 cut(s) 371
MaeII ACGT 1 cut(s) 296
MaeIII GTNAC 1 cut(s) 277
MalI GATC 2 cut(s) 69, 114
MboI GATC 2 cut(s) 67, 112
MboII GAAGA 4 cut(s) 41, 101, 122, 521
MhlI GDGCHC 1 cut(s) 522
MluCI AATT 5 cut(s) 235, 308, 414, 478, 495
Mly113I GGCGCC 1 cut(s) 285
MmeI TCCRAC 1 cut(s) 66
MnlI CCTC 9 cut(s) 25, 100, 130, 250, 315, 370, 377, 388, 463
MroXI GAANNNNTTC 1 cut(s) 128
MseI TTAA 1 cut(s) 477
MslI CAYNNNNRTG 1 cut(s) 458
MspI CCGG 1 cut(s) 282
MspR9I CCNGG 2 cut(s) 466, 484
MvaI CCWGG 2 cut(s) 466, 484
NarI GGCGCC 1 cut(s) 285
NdeII GATC 2 cut(s) 67, 112
NlaIII CATG 1 cut(s) 45
NlaIV GGNNCC 1 cut(s) 286
NmuCI GTSAC 1 cut(s) 277
PdmI GAANNNNTTC 1 cut(s) 128
PfeI GAWTC 1 cut(s) 354
PfoI TCCNGGA 1 cut(s) 482
PkrI GCNGC 2 cut(s) 176, 316
PluTI GGCGCC 1 cut(s) 288
PsiI TTATAA 1 cut(s) 420
Psp6I CCWGG 2 cut(s) 464, 482
PspFI CCCAGC 1 cut(s) 51
PspGI CCWGG 2 cut(s) 464, 482
PspN4I GGNNCC 1 cut(s) 286
RseI CAYNNNNRTG 1 cut(s) 458
SaqAI TTAA 1 cut(s) 477
SatI GCNGC 2 cut(s) 175, 315
Sau3AI GATC 2 cut(s) 67, 112
ScrFI CCNGG 2 cut(s) 466, 484
SduI GDGCHC 1 cut(s) 522
SetI ASST 6 cut(s) 53, 86, 129, 299, 344, 388
SfcI CTRYAG 1 cut(s) 13
SfoI GGCGCC 1 cut(s) 286
SmiMI CAYNNNNRTG 1 cut(s) 458
SmlI CTYRAG 3 cut(s) 326, 334, 379
SmoI CTYRAG 3 cut(s) 326, 334, 379
SpeI ACTAGT 1 cut(s) 370
Sse9I AATT 5 cut(s) 235, 308, 414, 478, 495
SsiI CCGC 1 cut(s) 219
SspDI GGCGCC 1 cut(s) 284
SspMI CTAG 1 cut(s) 371
StyD4I CCNGG 2 cut(s) 464, 482
TaaI ACNGT 3 cut(s) 161, 246, 259
TaiI ACGT 1 cut(s) 299
TaqI TCGA 1 cut(s) 23
TasI AATT 5 cut(s) 235, 308, 414, 478, 495
TfiI GAWTC 1 cut(s) 354
Tru1I TTAA 1 cut(s) 477
Tru9I TTAA 1 cut(s) 477
TscAI CASTG 2 cut(s) 352, 443
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 2 cut(s) 174, 314
Tsp45I GTSAC 1 cut(s) 277
TspRI CASTG 2 cut(s) 352, 443
XmiI GTMKAC 1 cut(s) 299
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.