Rh5CG277200

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0017815
Physical Location & Seq
Reverse (-)
30156864 .. 30159036
2173 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG277200.1

Sequence Viewer

Length: 1035 bp
ATGGTGGGAGTTTTGGATCATTTCCGGATGTCCATGACGGGTTTGTTTCTGTACATGTGTTTTGGTGAGAAGATGGAGGAGGAGAAGGTGAGAGAAATTGTGGACGTTCAGCGTCGGCTTCTGTTGGGTTTGGTCGGAGAGTTTCAGATTCTCAACTTTGGGCCTGCATGGTTTACCAAGATTTTGTTTTATAAGCGGTGGAGTCGCTTCTTCCAGATTCACCAGCAGCAAATAAACCTCCTGCTGCCTCTGATACAAGCACGGAAGAAGATAAAGAAAAGAAAATGCTCAAGTAACCGAAAAGAAGATAACAACAACAATCAAGCATTTGTTGATACCTTAATGGATTTAGAACTCCCCGACGAGAAACGAACATTCGATGAAGTAGAAGTGATGAACCAATGCTCGGATTTCCTCAACTCCGGCATAGACACAATTGCCATAGCACTGCAATGGATGATGGCAAACATAGTGAAACACCCACACATTCAAGACAAACTTGTCACAGAAATTAGACAAATTGTGGGAGATGGAGAAGTTGAAGAGGTGGAAGAGGAGAACCTGCACAGAATGAAGTATTTAAAAGCGGTTGTGCTAGAAGGTCTGCGTCTTCACCCGCCGTCGCACTTTTTGCTTCCGCACTCTGTCACTGAGGACGTCATTTTGGACGGGCGTTACGTTGTGCCAAAGGACTGCACTGTGAACTTCTCCGTGGCGGAGATGGGGCGGGATCCGAAGGTGTGGGCGGAGCCGATGGAGTTCAAGCCTGAGAGGTTTCTGAGTGATGAGGAGAACTTGTGTGATTTAACTGGTAGTAAGGAGATCAAGATGATGCCGTTTGGGGTAGGGAGGAGGATGTGTCCTGGTTCGAGTTTGGCAATGCTGCTTTTGGAGTACTTTGTGGCGAATTTGGTTTGGAAATTCGAGTGGAGAGCTGTGGAGGGAGTTGGGGTTGATTTGAGTGAGAAGGAGGAGACGAGTTGGGGAATGAATAATCCATTGCAGGCACATATCACTCCAAGGTTGAAGACATGA

Protein Analysis

344

Amino Acids

40.0

Weight (kDa)

5.7

Isoelectric Point (pI)

42.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 9 - 327 6.1e-69 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017815)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0035991
rosa_laevigata RLG00000033658
rosa_multiflora Rmu_co8295021.1_g000001 Rmu_sc0000084.1_g000043 Rmu_sc0001493.1_g000014
rosa_roxburghii Rroxscaffold_1G00044750
rosa_rugosa Rorug05G0152000
rosa_samantha Rh5AG246000 Rh5BG246400 Rh5CG277200 Rh5DG254900
rosa_wichuraiana Rw5G022600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 192
AasI GACNNNNNNGTC 1 cut(s) 500
AatII GACGTC 1 cut(s) 660
Acc36I ACCTGC 1 cut(s) 570
AccIII TCCGGA 1 cut(s) 24
AciI CCGC 7 cut(s) 196, 587, 617, 638, 716, 727, 746
AclWI GGATC 3 cut(s) 24, 725, 738
AcsI RAATTY 2 cut(s) 907, 920
AcyI GRCGYC 1 cut(s) 657
AfaI GTAC 2 cut(s) 53, 896
AfiI CCNNNNNNNGG 1 cut(s) 406
AflIII ACRYGT 1 cut(s) 54
AgsI TTSAA 4 cut(s) 491, 542, 763, 1027
AjnI CCWGG 1 cut(s) 862
AluBI AGCT 1 cut(s) 935
AluI AGCT 1 cut(s) 935
Alw26I GTCTC 1 cut(s) 968
AlwI GGATC 3 cut(s) 24, 725, 738
Aor13HI TCCGGA 1 cut(s) 24
AoxI GGCC 1 cut(s) 161
ApeKI GCWGC 3 cut(s) 226, 244, 883
ApoI RAATTY 2 cut(s) 907, 920
AspS9I GGNCC 1 cut(s) 161
AsuHPI GGTGA 4 cut(s) 77, 100, 212, 605
BamHI GGATCC 1 cut(s) 730
BbsI GAAGAC 1 cut(s) 602
BbvI GCAGC 3 cut(s) 231, 238, 870
BccI CCATC 5 cut(s) 67, 454, 524, 715, 748
BceAI ACGGC 2 cut(s) 604, 820
BciT130I CCWGG 1 cut(s) 864
BcoDI GTCTC 1 cut(s) 968
BfaI CTAG 1 cut(s) 596
BfuAI ACCTGC 1 cut(s) 570
BisI GCNGC 3 cut(s) 227, 245, 884
BlsI GCNGC 3 cut(s) 228, 246, 885
BmcAI AGTACT 1 cut(s) 896
Bme1390I CCNGG 1 cut(s) 864
BmgT120I GGNCC 1 cut(s) 161
BmiI GGNNCC 2 cut(s) 732, 750
BmrFI CCNGG 1 cut(s) 864
BmsI GCATC 1 cut(s) 822
BpiI GAAGAC 1 cut(s) 602
BpuEI CTTGAG 1 cut(s) 274
BsaHI GRCGYC 1 cut(s) 657
BsaJI CCNNGG 2 cut(s) 711, 1019
BsaWI WCCGGW 1 cut(s) 24
BsaXI ACNNNNNCTCC 6 cut(s) 782, 812, 844, 874, 936, 966
Bsc4I CCNNNNNNNGG 1 cut(s) 406
Bse1I ACTGG 1 cut(s) 814
Bse3DI GCAATG 3 cut(s) 458, 885, 998
BseAI TCCGGA 1 cut(s) 24
BseBI CCWGG 1 cut(s) 864
BseDI CCNNGG 2 cut(s) 711, 1019
BseGI GGATG 3 cut(s) 33, 462, 861
BseLI CCNNNNNNNGG 1 cut(s) 406
BseMI GCAATG 3 cut(s) 458, 885, 998
BseMII CTCAG 3 cut(s) 642, 759, 770
BseNI ACTGG 1 cut(s) 814
BseRI GAGGAG 6 cut(s) 92, 95, 569, 803, 865, 986
BseXI GCAGC 3 cut(s) 231, 238, 870
BsgI GTGCAG 2 cut(s) 548, 679
BshFI GGCC 1 cut(s) 163
BsiSI CCGG 2 cut(s) 25, 423
BslI CCNNNNNNNGG 1 cut(s) 406
BsmAI GTCTC 1 cut(s) 968
BsmBI CGTCTC 1 cut(s) 968
BsnI GGCC 1 cut(s) 163
Bsp13I TCCGGA 1 cut(s) 24
Bsp1407I TGTACA 1 cut(s) 51
Bsp143I GATC 3 cut(s) 16, 730, 822
BspACI CCGC 7 cut(s) 196, 587, 617, 638, 716, 727, 746
BspANI GGCC 1 cut(s) 163
BspCNI CTCAG 3 cut(s) 643, 760, 771
BspEI TCCGGA 1 cut(s) 24
BspLI GGNNCC 2 cut(s) 732, 750
BspMI ACCTGC 1 cut(s) 570
BspPI GGATC 3 cut(s) 24, 725, 738
BsrDI GCAATG 3 cut(s) 458, 885, 998
BsrGI TGTACA 1 cut(s) 51
BsrI ACTGG 1 cut(s) 814
BssECI CCNNGG 2 cut(s) 711, 1019
BssMI GATC 3 cut(s) 16, 730, 822
BssNI GRCGYC 1 cut(s) 657
BssT1I CCWWGG 1 cut(s) 1019
Bst2UI CCWGG 1 cut(s) 864
Bst4CI ACNGT 1 cut(s) 700
Bst6I CTCTTC 2 cut(s) 537, 546
BstACI GRCGYC 1 cut(s) 657
BstAPI GCANNNNNTGC 1 cut(s) 631
BstAUI TGTACA 1 cut(s) 51
BstC8I GCNNGC 2 cut(s) 165, 1005
BstDEI CTNAG 3 cut(s) 651, 768, 779
BstDSI CCRYGG 1 cut(s) 711
BstF5I GGATG 3 cut(s) 33, 462, 861
BstKTI GATC 3 cut(s) 19, 733, 825
BstMAI GTCTC 1 cut(s) 968
BstMBI GATC 3 cut(s) 16, 730, 822
BstMWI GCNNNNNNNGC 1 cut(s) 631
BstNI CCWGG 1 cut(s) 864
BstNSI RCATGY 1 cut(s) 58
BstSCI CCNGG 1 cut(s) 862
BstV1I GCAGC 3 cut(s) 231, 238, 870
BstV2I GAAGAC 1 cut(s) 602
BstX2I RGATCY 1 cut(s) 730
BstYI RGATCY 1 cut(s) 730
BsuRI GGCC 1 cut(s) 163
BtgI CCRYGG 1 cut(s) 711
BtsCI GGATG 3 cut(s) 33, 462, 861
BtsI GCAGTG 1 cut(s) 446
BtsIMutI CAGTG 3 cut(s) 446, 648, 696
BveI ACCTGC 1 cut(s) 570
Cac8I GCNNGC 2 cut(s) 165, 1005
Cfr13I GGNCC 1 cut(s) 161
CseI GACGC 2 cut(s) 101, 596
Csp6I GTAC 2 cut(s) 52, 895
CviAII CATG 4 cut(s) 34, 55, 168, 1032
CviJI RGCY 5 cut(s) 118, 163, 751, 766, 935
CviKI_1 RGCY 5 cut(s) 118, 163, 751, 766, 935
CviQI GTAC 2 cut(s) 52, 895
DdeI CTNAG 3 cut(s) 651, 768, 779
DpnI GATC 3 cut(s) 18, 732, 824
DpnII GATC 3 cut(s) 16, 730, 822
DraI TTTAAA 1 cut(s) 582
DrdI GACNNNNNNGTC 1 cut(s) 500
DseDI GACNNNNNNGTC 1 cut(s) 500
Eam1104I CTCTTC 2 cut(s) 537, 546
EarI CTCTTC 2 cut(s) 537, 546
EciI GGCGGA 2 cut(s) 731, 761
Eco130I CCWWGG 1 cut(s) 1019
EcoRII CCWGG 1 cut(s) 862
EcoT14I CCWWGG 1 cut(s) 1019
ErhI CCWWGG 1 cut(s) 1019
Esp3I CGTCTC 1 cut(s) 968
FaeI CATG 4 cut(s) 37, 58, 171, 1035
FaiI YATR 9 cut(s) 35, 56, 169, 192, 428, 443, 470, 1011, 1033
FalI AAGNNNNNCTT 2 cut(s) 483, 515
FatI CATG 4 cut(s) 33, 54, 167, 1031
FauI CCCGC 2 cut(s) 624, 720
Fnu4HI GCNGC 3 cut(s) 227, 245, 884
FokI GGATG 3 cut(s) 40, 469, 868
Fsp4HI GCNGC 3 cut(s) 227, 245, 884
FspBI CTAG 1 cut(s) 596
GluI GCNGC 3 cut(s) 227, 245, 884
HaeIII GGCC 1 cut(s) 163
HapII CCGG 2 cut(s) 25, 423
HgaI GACGC 2 cut(s) 101, 596
Hin1I GRCGYC 1 cut(s) 657
Hin1II CATG 4 cut(s) 37, 58, 171, 1035
HinfI GANTC 3 cut(s) 148, 202, 217
HpaII CCGG 2 cut(s) 25, 423
HphI GGTGA 4 cut(s) 77, 100, 212, 605
Hpy166II GTNNAC 3 cut(s) 103, 174, 703
Hpy188I TCNGA 6 cut(s) 137, 147, 252, 409, 735, 780
Hpy188III TCNNGA 4 cut(s) 25, 214, 491, 826
Hpy8I GTNNAC 3 cut(s) 103, 174, 703
Hpy99I CGWCG 3 cut(s) 117, 365, 625
HpyAV CCTTC 4 cut(s) 79, 593, 730, 961
HpyCH4III ACNGT 1 cut(s) 700
HpyCH4IV ACGT 3 cut(s) 105, 657, 678
HpyCH4V TGCA 5 cut(s) 167, 451, 565, 696, 1003
HpyF10VI GCNNNNNNNGC 1 cut(s) 631
HpyF3I CTNAG 3 cut(s) 651, 768, 779
HpySE526I ACGT 3 cut(s) 105, 657, 678
Hsp92I GRCGYC 1 cut(s) 657
Hsp92II CATG 4 cut(s) 37, 58, 171, 1035
Kpn2I TCCGGA 1 cut(s) 24
Kzo9I GATC 3 cut(s) 16, 730, 822
LmnI GCTCC 1 cut(s) 748
Lsp1109I GCAGC 3 cut(s) 231, 238, 870
LweI GCATC 1 cut(s) 822
MaeI CTAG 1 cut(s) 596
MaeII ACGT 3 cut(s) 105, 657, 678
MaeIII GTNAC 4 cut(s) 293, 502, 646, 674
MalI GATC 3 cut(s) 18, 732, 824
MboI GATC 3 cut(s) 16, 730, 822
MboII GAAGA 8 cut(s) 82, 202, 277, 280, 317, 554, 563, 602
MfeI CAATTG 1 cut(s) 435
MflI RGATCY 1 cut(s) 730
MluCI AATT 6 cut(s) 96, 435, 510, 519, 907, 920
MlyI GAGTC 1 cut(s) 211
MmeI TCCRAC 1 cut(s) 115
MroI TCCGGA 1 cut(s) 24
MseI TTAA 3 cut(s) 341, 581, 806
MslI CAYNNNNRTG 1 cut(s) 451
MspI CCGG 2 cut(s) 25, 423
MspR9I CCNGG 1 cut(s) 864
MunI CAATTG 1 cut(s) 435
MvaI CCWGG 1 cut(s) 864
MwoI GCNNNNNNNGC 1 cut(s) 631
NdeII GATC 3 cut(s) 16, 730, 822
NlaIII CATG 4 cut(s) 37, 58, 171, 1035
NlaIV GGNNCC 2 cut(s) 732, 750
NmuCI GTSAC 2 cut(s) 502, 646
NspI RCATGY 1 cut(s) 58
PciI ACATGT 1 cut(s) 54
PcsI WCGNNNNNNNCGW 1 cut(s) 675
PfeI GAWTC 2 cut(s) 148, 217
PkrI GCNGC 3 cut(s) 228, 246, 885
PleI GAGTC 1 cut(s) 210
PpsI GAGTC 1 cut(s) 210
PscI ACATGT 1 cut(s) 54
PsiI TTATAA 1 cut(s) 192
Psp6I CCWGG 1 cut(s) 862
PspGI CCWGG 1 cut(s) 862
PspN4I GGNNCC 2 cut(s) 732, 750
PspPI GGNCC 1 cut(s) 161
PsuI RGATCY 1 cut(s) 730
RsaI GTAC 2 cut(s) 53, 896
RsaNI GTAC 2 cut(s) 52, 895
RseI CAYNNNNRTG 1 cut(s) 451
SaqAI TTAA 3 cut(s) 341, 581, 806
SatI GCNGC 3 cut(s) 227, 245, 884
Sau3AI GATC 3 cut(s) 16, 730, 822
Sau96I GGNCC 1 cut(s) 161
ScaI AGTACT 1 cut(s) 896
SchI GAGTC 1 cut(s) 211
ScrFI CCNGG 1 cut(s) 864
SfaNI GCATC 1 cut(s) 822
SmiMI CAYNNNNRTG 1 cut(s) 451
SmlI CTYRAG 1 cut(s) 289
SmoI CTYRAG 1 cut(s) 289
Sse9I AATT 6 cut(s) 96, 435, 510, 519, 907, 920
SsiI CCGC 7 cut(s) 196, 587, 617, 638, 716, 727, 746
SspMI CTAG 1 cut(s) 596
StyD4I CCNGG 1 cut(s) 862
StyI CCWWGG 1 cut(s) 1019
TaaI ACNGT 1 cut(s) 700
TaiI ACGT 3 cut(s) 108, 660, 681
TaqI TCGA 3 cut(s) 378, 869, 924
TasI AATT 6 cut(s) 96, 435, 510, 519, 907, 920
TatI WGTACW 2 cut(s) 51, 894
TfiI GAWTC 2 cut(s) 148, 217
Tru1I TTAA 3 cut(s) 341, 581, 806
Tru9I TTAA 3 cut(s) 341, 581, 806
TscAI CASTG 3 cut(s) 453, 655, 703
TseFI GTSAC 2 cut(s) 502, 646
TseI GCWGC 3 cut(s) 226, 244, 883
Tsp45I GTSAC 2 cut(s) 502, 646
TspDTI ATGAA 4 cut(s) 396, 410, 587, 1004
TspGWI ACGGA 2 cut(s) 277, 700
TspRI CASTG 3 cut(s) 453, 655, 703
XapI RAATTY 2 cut(s) 907, 920
XceI RCATGY 1 cut(s) 58
XspI CTAG 1 cut(s) 596
ZraI GACGTC 1 cut(s) 658
ZrmI AGTACT 1 cut(s) 896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.