MD07G1251100.v1.1

DYAD-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001779
Physical Location & Seq
Forward (+)
31940910 .. 31944124
3215 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1251100.v1.1.491

Sequence Viewer

Length: 2352 bp
ATGAGGAAGTACTTGGGGAAGAAGCAAGTCCAAAAAAAGCCAGAAAGCAAAAGCACTTTTTTTGCCAGACATGCTCTGCCCTCCCCTGCTTCTGCTTCTCCTTCTTCCTCCCCAGCTTCTTCGCCCGATGCTATCGAGCACATTAAAGTCGGATGTTTCTACCAAATCGACCAATCGAAGCTCCATGAAAGAACCGTACCGGAGCAGCTCAAGGACGTACGGATCGTCATGGTGAGTGCGAAGGGTGCGTTTAAAGTGGCAGTGCGGTTTCCGAGCCTTCACTCTCTGCGCACTCACATCAACGAAAATGGGTACCCAAAACCAGATGCCAAGCAGATTCCGGCGCTCGACGAGAAGTACGTGATGTCGCCGGATATGGCATCAGAGGTACTTTACCGGAGAATCCCACCTCAGGAGATGGTTGATCAGAGCAAAATTTGGAGTTTCTGGGCCATTCGACCTCCGTTGAAGAATCACGCGAGATGCTCGTCGTCGAGCCCAGCAATCAGTGGCGGCGGCGGAGCCATGGATAGTAGCGTGGTTTCTAGGAAGGGCCCGTGTTCGTCCGAGCTCGCATTTGCCGGAATGGTGAAGTGGGGCAAGCGGCGGCAGATTCGCTACCTTCGCAGGCACGAGTCGTCGCCGAGCTCTGGCTCTGAAGATTCAGAGAAGGAAAAGAAGGTAATGGAGCTGGAGGACGGTGACGAAGAAGAAGGCGAGGAGACAGAGGAAGAAGCTCATCTTCCTCCTGTTCTGCCATGGAAGATGAACCTCCGGAATCGGAAGCGGAAGCGCCCTGATAACAAGAGAAATATCAAAGCTCCGACTCTGAAGCGATCAAAATCCAGAGCAAAAAGTGCGAAGCAGAATAACCAGATTGTAGCAGTTCCTGATCGCAGCAGCAGAAAATTGGCTAAAGTTAAACACACTGTAGCAAGATGGTCAGCAGGAAGGTACAAGCTGGCGGAGGAGAACATGTTGAAGGTGATGAACGAGAAGGGGGCGGCGATTGGGAACCCAATACGGAGGCCGGCGCTGAGAGCGGAGGCTCGGAGGCTGATTGGGGACACGGGTTTACTGGATCATTTACTCAAACACATGGCGGGCAAGGTGGCGCCTGGCGGGGCCGAGAGGTTTAGACGGCGGCACAACGCTGATGGGGCCATGGAATATTGGCTGGAGAGTGCTGATCTGGTCCACATCCGAAAGGAAGCTGGGGTTCAAGATCCCTACTGGACCCCGCCGCCGGGGTGGAAGCCCGGCGATAGCCCCACCCAGGACCCCACTTGTGCAAGAGAGATTAAGGAGCTCCGTGAGGAAATTGACAAGATTAGAAGGGAGATGGAGGAGTCCAAGCAGCAAGGGAATGCCGGTTTGGCTATGGCGACCTCTTCAAATTCTTGTTTGACCGGTGTTGACTTGGATCGTGATGGTTCGTTAATCCAGATTAAGGAGGCTTGTGCAGAGCTAGAAAAGAAGATAGCTAAAATGGATGAAGAAACGATGGAGTTTGTGACCAAAAAAGAAGAACAAATGGTGGAGTTTACTCAATCTTTGTCCGGAATTAAGGAGAAAATCAGGATGTTTGAATTAAGGAAGGAAGGTGCAGTCACAATTTCAGGAGCACCACCGCCATCTGAAACAGCATCAGCCGCTGAGAAGAAAGAAGAAGATAAACTAAGCGGCGGAGACACGGCGGCTGCGGCGGCGGAGGACAAGGCGGCAAAAATAGATAGGCTGAGGAGTGGGTTCAGGATATGCAAACCACAAGGTAGCTTTCTCTGGCCAGACATGGGCAGCCCCACCAATATTCCCAGTCCTTCTTCTCAGGTTCTGGCCCCACCACATGACCCCTTTGTGGTTCCCACCCCACCCTCATCCACCTCCTCCGCCCCCGGCCACCTCATCAGCCCCACCACTCCACCTGTGAAGCCGGTAGCTGAGCGGGGCCCAGTGACCACCACCACATTGTGCAATGTGACCACAATACCCTCTGCCCCTCCGATTTTCGCTCCTCCACCTGTCCTGGCCCCACAGCAAACTCAGAGTTCCACTACCATCATCATCAGTAAAACCTTACTCTTAAACCTCAACGAGCTCCCTGCAGTTGCAGCACCAAACCCTAACCCTAACCCTAACCCCCAAACCAGCTGTGAGGCTACCGGGACTGTCACCACCTATAACAGAATGCACCACCACCATCTGCCACGTATGGTGGAGGAGAAGGAGGAGGACGACGGAGATGCAAGAAACGGTGATGATTGGTGTGCCTCTCCTGCTTCCACGTGGAAGGAACAGAAGTGGTTGATGGCTTTGGCTGTGGGAACTCCTGGCACTTCTTTGGAGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0007049 GO:0008150 GO:0009987 GO:0022414 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044424 GO:0044464 GO:0051321
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

784

Amino Acids

86.2

Weight (kDa)

9.1

Isoelectric Point (pI)

63.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_plant_repro PF25874 313 - 395 5.2e-25 Winged helix-turn-helix domain in plant reproductive proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 290
Acc65I GGTACC 1 cut(s) 312
AccB1I GGYRCC 2 cut(s) 312, 1114
AccBSI CCGCTC 2 cut(s) 1043, 1945
AccII CGCG 1 cut(s) 479
AccIII TCCGGA 2 cut(s) 774, 1559
AclWI GGATC 4 cut(s) 230, 1089, 1220, 1431
AcoI YGGCCR 2 cut(s) 1784, 1897
AcsI RAATTY 2 cut(s) 435, 1396
AcuI CTGAAG 2 cut(s) 678, 851
AcvI CACGTG 1 cut(s) 2286
AcyI GRCGYC 1 cut(s) 1115
AdeI CACNNNGTG 1 cut(s) 1971
AfaI GTAC 7 cut(s) 11, 198, 219, 314, 359, 390, 956
AfiI CCNNNNNNNGG 8 cut(s) 412, 650, 1246, 1247, 1276, 1450, 1793, 1858
AflIII ACRYGT 1 cut(s) 975
AgeI ACCGGT 1 cut(s) 1409
AgsI TTSAA 5 cut(s) 469, 982, 1223, 1395, 1589
AjnI CCWGG 4 cut(s) 1117, 1275, 2025, 2329
AjuI GAANNNNNNNTTGG 2 cut(s) 1358, 1390
Alw21I GWGCWC 6 cut(s) 141, 573, 650, 1311, 1627, 2100
Alw26I GTCTC 2 cut(s) 716, 1683
AlwI GGATC 4 cut(s) 230, 1089, 1220, 1431
AlwNI CAGNNNCTG 3 cut(s) 890, 1655, 1834
Aor13HI TCCGGA 2 cut(s) 774, 1559
ApaI GGGCCC 2 cut(s) 557, 1952
ApeKI GCWGC 7 cut(s) 205, 897, 900, 1357, 1700, 1797, 2111
ApoI RAATTY 2 cut(s) 435, 1396
AsiGI ACCGGT 1 cut(s) 1409
Asp718I GGTACC 1 cut(s) 312
AspLEI GCGC 5 cut(s) 291, 346, 795, 1036, 1117
AsuC2I CCSGG 4 cut(s) 1248, 1260, 1896, 2164
AsuHPI GGTGA 6 cut(s) 244, 601, 713, 997, 2164, 2267
AvaII GGWCC 3 cut(s) 1195, 1236, 1279
AxyI CCTNAGG 1 cut(s) 411
BaeGI GKGCMC 2 cut(s) 557, 1952
BalI TGGCCA 1 cut(s) 1786
BanI GGYRCC 2 cut(s) 312, 1114
BanII GRGCYC 7 cut(s) 500, 557, 573, 650, 1311, 1952, 2100
BauI CACGAG 1 cut(s) 632
BbrPI CACGTG 1 cut(s) 2286
Bbv12I GWGCWC 6 cut(s) 141, 573, 650, 1311, 1627, 2100
BbvCI CCTCAGC 1 cut(s) 1739
BbvI GCAGC 7 cut(s) 217, 909, 912, 1369, 1687, 1809, 2123
BceAI ACGGC 2 cut(s) 1157, 1710
BcgI CGANNNNNNTGC 4 cut(s) 2084, 2118, 2225, 2259
BciT130I CCWGG 4 cut(s) 1119, 1277, 2027, 2331
BclI TGATCA 1 cut(s) 424
BcnI CCSGG 4 cut(s) 1248, 1260, 1896, 2164
BcoDI GTCTC 2 cut(s) 716, 1683
BfaI CTAG 2 cut(s) 546, 1469
BfmI CTRYAG 2 cut(s) 930, 2103
BfoI RGCGCY 4 cut(s) 347, 796, 1037, 1118
BglI GCCNNNNNGGC 1 cut(s) 1376
BlpI GCTNAGC 1 cut(s) 1941
BmcAI AGTACT 1 cut(s) 11
Bme1390I CCNGG 8 cut(s) 1119, 1248, 1260, 1277, 1896, 2027, 2164, 2331
Bme18I GGWCC 3 cut(s) 1195, 1236, 1279
BmrFI CCNGG 8 cut(s) 1119, 1248, 1260, 1277, 1896, 2027, 2164, 2331
BmrI ACTGGG 2 cut(s) 1809, 1946
BmsI GCATC 6 cut(s) 118, 316, 389, 473, 1655, 2233
BmuI ACTGGG 2 cut(s) 1809, 1946
BpmI CTGGAG 2 cut(s) 713, 1199
Bpu10I CCTNAGC 1 cut(s) 1739
Bpu1102I GCTNAGC 1 cut(s) 1941
BpuEI CTTGAG 1 cut(s) 194
BpuMI CCSGG 4 cut(s) 1248, 1260, 1896, 2164
BsaAI YACGTR 3 cut(s) 361, 2210, 2286
BsaBI GATNNNNATC 1 cut(s) 841
BsaHI GRCGYC 1 cut(s) 1115
BsaJI CCNNGG 6 cut(s) 525, 758, 1164, 1247, 1275, 1894
BsaWI WCCGGW 5 cut(s) 199, 396, 774, 1409, 1559
BsaXI ACNNNNNCTCC 2 cut(s) 962, 992
Bsc4I CCNNNNNNNGG 8 cut(s) 412, 650, 1246, 1247, 1276, 1450, 1793, 1858
Bse118I RCCGGY 4 cut(s) 1030, 1370, 1409, 1933
Bse1I ACTGG 4 cut(s) 1083, 1238, 1815, 1952
Bse21I CCTNAGG 1 cut(s) 411
Bse3DI GCAATG 1 cut(s) 1981
Bse8I GATNNNNATC 1 cut(s) 841
BseAI TCCGGA 2 cut(s) 774, 1559
BseBI CCWGG 4 cut(s) 1119, 1277, 2027, 2331
BseDI CCNNGG 6 cut(s) 525, 758, 1164, 1247, 1275, 1894
BseGI GGATG 5 cut(s) 158, 1200, 1498, 1587, 1877
BseJI GATNNNNATC 1 cut(s) 841
BseLI CCNNNNNNNGG 8 cut(s) 412, 650, 1246, 1247, 1276, 1450, 1793, 1858
BseMI GCAATG 1 cut(s) 1981
BseMII CTCAG 7 cut(s) 425, 1028, 1647, 1730, 1841, 1932, 2057
BseNI ACTGG 4 cut(s) 1083, 1238, 1815, 1952
BseRI GAGGAG 8 cut(s) 734, 983, 1361, 1756, 1876, 2004, 2234, 2243
BseSI GKGCMC 2 cut(s) 557, 1952
BseXI GCAGC 7 cut(s) 217, 909, 912, 1369, 1687, 1809, 2123
BseYI CCCAGC 3 cut(s) 112, 499, 1214
BsgI GTGCAG 2 cut(s) 1482, 1626
Bsh1236I CGCG 1 cut(s) 479
BshNI GGYRCC 2 cut(s) 312, 1114
BshTI ACCGGT 1 cut(s) 1409
BsiHKAI GWGCWC 6 cut(s) 141, 573, 650, 1311, 1627, 2100
BsiWI CGTACG 1 cut(s) 217
BslFI GGGAC 2 cut(s) 1079, 2179
BslI CCNNNNNNNGG 8 cut(s) 412, 650, 1246, 1247, 1276, 1450, 1793, 1858
BsmAI GTCTC 2 cut(s) 716, 1683
BsmFI GGGAC 2 cut(s) 1079, 2179
BsmI GAATGC 2 cut(s) 1372, 2193
Bsp120I GGGCCC 2 cut(s) 553, 1948
Bsp1286I GDGCHC 9 cut(s) 141, 500, 557, 573, 650, 1311, 1627, 1952, 2100
Bsp13I TCCGGA 2 cut(s) 774, 1559
Bsp143I GATC 8 cut(s) 222, 424, 836, 892, 1081, 1189, 1225, 1423
Bsp1720I GCTNAGC 1 cut(s) 1941
Bsp19I CCATGG 3 cut(s) 525, 758, 1164
BspCNI CTCAG 7 cut(s) 424, 1029, 1648, 1731, 1840, 1933, 2056
BspEI TCCGGA 2 cut(s) 774, 1559
BspFNI CGCG 1 cut(s) 479
BspMAI CTGCAG 1 cut(s) 2107
BspPI GGATC 4 cut(s) 230, 1089, 1220, 1431
BspT107I GGYRCC 2 cut(s) 312, 1114
BsrBI CCGCTC 2 cut(s) 1043, 1945
BsrDI GCAATG 1 cut(s) 1981
BsrFI RCCGGY 4 cut(s) 1030, 1370, 1409, 1933
BsrI ACTGG 4 cut(s) 1083, 1238, 1815, 1952
BssAI RCCGGY 4 cut(s) 1030, 1370, 1409, 1933
BssECI CCNNGG 6 cut(s) 525, 758, 1164, 1247, 1275, 1894
BssMI GATC 8 cut(s) 222, 424, 836, 892, 1081, 1189, 1225, 1423
BssNI GRCGYC 1 cut(s) 1115
BssSI CACGAG 1 cut(s) 632
BssT1I CCWWGG 3 cut(s) 525, 758, 1164
Bst2BI CACGAG 1 cut(s) 632
Bst2UI CCWGG 4 cut(s) 1119, 1277, 2027, 2331
Bst4CI ACNGT 5 cut(s) 196, 701, 931, 2170, 2255
Bst6I CTCTTC 1 cut(s) 1396
BstACI GRCGYC 1 cut(s) 1115
BstAPI GCANNNNNTGC 1 cut(s) 857
BstBAI YACGTR 3 cut(s) 361, 2210, 2286
BstC8I GCNNGC 6 cut(s) 573, 602, 629, 963, 1032, 1105
BstDEI CTNAG 8 cut(s) 411, 1037, 1656, 1679, 1739, 1827, 1941, 2043
BstDSI CCRYGG 3 cut(s) 525, 758, 1164
BstF5I GGATG 5 cut(s) 158, 1200, 1498, 1587, 1877
BstFNI CGCG 1 cut(s) 479
BstH2I RGCGCY 4 cut(s) 347, 796, 1037, 1118
BstHHI GCGC 5 cut(s) 291, 346, 795, 1036, 1117
BstKTI GATC 8 cut(s) 225, 427, 839, 895, 1084, 1192, 1228, 1426
BstMAI GTCTC 2 cut(s) 716, 1683
BstMBI GATC 8 cut(s) 222, 424, 836, 892, 1081, 1189, 1225, 1423
BstNI CCWGG 4 cut(s) 1119, 1277, 2027, 2331
BstNSI RCATGY 2 cut(s) 74, 979
BstSCI CCNGG 8 cut(s) 1117, 1246, 1258, 1275, 1894, 2025, 2162, 2329
BstSFI CTRYAG 2 cut(s) 930, 2103
BstSLI GKGCMC 2 cut(s) 557, 1952
BstUI CGCG 1 cut(s) 479
BstV1I GCAGC 7 cut(s) 217, 909, 912, 1369, 1687, 1809, 2123
BstX2I RGATCY 1 cut(s) 1225
BstYI RGATCY 1 cut(s) 1225
Bsu36I CCTNAGG 1 cut(s) 411
BtgI CCRYGG 3 cut(s) 525, 758, 1164
BtsCI GGATG 5 cut(s) 158, 1200, 1498, 1587, 1877
BtsI GCAGTG 1 cut(s) 267
BtsIMutI CAGTG 4 cut(s) 267, 514, 927, 1959
Cac8I GCNNGC 6 cut(s) 573, 602, 629, 963, 1032, 1105
CaiI CAGNNNCTG 3 cut(s) 890, 1655, 1834
CfoI GCGC 5 cut(s) 291, 346, 795, 1036, 1117
Cfr10I RCCGGY 4 cut(s) 1030, 1370, 1409, 1933
Csp6I GTAC 7 cut(s) 10, 197, 218, 313, 358, 389, 955
CspAI ACCGGT 1 cut(s) 1409
CviQI GTAC 7 cut(s) 10, 197, 218, 313, 358, 389, 955
DdeI CTNAG 8 cut(s) 411, 1037, 1656, 1679, 1739, 1827, 1941, 2043
DinI GGCGCC 1 cut(s) 1116
DpnI GATC 8 cut(s) 224, 426, 838, 894, 1083, 1191, 1227, 1425
DpnII GATC 8 cut(s) 222, 424, 836, 892, 1081, 1189, 1225, 1423
DraI TTTAAA 1 cut(s) 253
DraIII CACNNNGTG 1 cut(s) 1971
EaeI YGGCCR 2 cut(s) 1784, 1897
Eam1104I CTCTTC 1 cut(s) 1396
EarI CTCTTC 1 cut(s) 1396
EciI GGCGGA 5 cut(s) 534, 980, 1701, 1724, 1879
Ecl136II GAGCTC 4 cut(s) 571, 648, 1309, 2098
Eco130I CCWWGG 3 cut(s) 525, 758, 1164
Eco24I GRGCYC 7 cut(s) 500, 557, 573, 650, 1311, 1952, 2100
Eco47I GGWCC 3 cut(s) 1195, 1236, 1279
Eco53kI GAGCTC 4 cut(s) 571, 648, 1309, 2098
Eco57I CTGAAG 2 cut(s) 678, 851
Eco72I CACGTG 1 cut(s) 2286
Eco81I CCTNAGG 1 cut(s) 411
EcoICRI GAGCTC 4 cut(s) 571, 648, 1309, 2098
EcoO109I RGGNCCY 3 cut(s) 553, 1279, 1948
EcoRII CCWGG 4 cut(s) 1117, 1275, 2025, 2329
EcoT14I CCWWGG 3 cut(s) 525, 758, 1164
EcoT38I GRGCYC 7 cut(s) 500, 557, 573, 650, 1311, 1952, 2100
EgeI GGCGCC 1 cut(s) 1116
EheI GGCGCC 1 cut(s) 1116
ErhI CCWWGG 3 cut(s) 525, 758, 1164
FalI AAGNNNNNCTT 2 cut(s) 726, 758
FaqI GGGAC 2 cut(s) 1079, 2179
FauI CCCGC 4 cut(s) 1096, 1115, 1248, 1938
FbaI TGATCA 1 cut(s) 424
FokI GGATG 5 cut(s) 165, 1187, 1505, 1594, 1864
FriOI GRGCYC 7 cut(s) 500, 557, 573, 650, 1311, 1952, 2100
FspBI CTAG 2 cut(s) 546, 1469
FspI TGCGCA 1 cut(s) 290
GlaI GCGC 5 cut(s) 290, 345, 794, 1035, 1116
GsaI CCCAGC 3 cut(s) 116, 503, 1218
GsuI CTGGAG 2 cut(s) 713, 1199
HaeII RGCGCY 4 cut(s) 347, 796, 1037, 1118
HhaI GCGC 5 cut(s) 291, 346, 795, 1036, 1117
Hin1I GRCGYC 1 cut(s) 1115
Hin6I GCGC 5 cut(s) 289, 344, 793, 1034, 1115
HinP1I GCGC 5 cut(s) 289, 344, 793, 1034, 1115
HincII GTYRAC 1 cut(s) 1417
HindII GTYRAC 1 cut(s) 1417
HinfI GANTC 9 cut(s) 337, 402, 472, 613, 635, 662, 778, 826, 1349
HphI GGTGA 6 cut(s) 244, 601, 713, 997, 2164, 2267
Hpy166II GTNNAC 4 cut(s) 1076, 1198, 1417, 1545
Hpy8I GTNNAC 4 cut(s) 1076, 1198, 1417, 1545
Hpy99I CGWCG 5 cut(s) 353, 493, 496, 643, 2240
HpyCH4III ACNGT 5 cut(s) 196, 701, 931, 2170, 2255
HpyCH4IV ACGT 4 cut(s) 216, 360, 2209, 2285
HpyCH4V TGCA 9 cut(s) 1292, 1463, 1607, 1761, 1974, 2105, 2111, 2191, 2246
HpyF3I CTNAG 8 cut(s) 411, 1037, 1656, 1679, 1739, 1827, 1941, 2043
HpySE526I ACGT 4 cut(s) 216, 360, 2209, 2285
Hsp92I GRCGYC 1 cut(s) 1115
HspAI GCGC 5 cut(s) 289, 344, 793, 1034, 1115
KasI GGCGCC 1 cut(s) 1114
Kpn2I TCCGGA 2 cut(s) 774, 1559
KpnI GGTACC 1 cut(s) 316
KroI GCCGGC 1 cut(s) 1030
KroNI GCCGGC 1 cut(s) 1032
Ksp22I TGATCA 1 cut(s) 424
Kzo9I GATC 8 cut(s) 222, 424, 836, 892, 1081, 1189, 1225, 1423
Lsp1109I GCAGC 7 cut(s) 217, 909, 912, 1369, 1687, 1809, 2123
LweI GCATC 6 cut(s) 118, 316, 389, 473, 1655, 2233
MaeI CTAG 2 cut(s) 546, 1469
MaeII ACGT 4 cut(s) 216, 360, 2209, 2285
MaeIII GTNAC 6 cut(s) 701, 1513, 1609, 1954, 1978, 2170
MalI GATC 8 cut(s) 224, 426, 838, 894, 1083, 1191, 1227, 1425
MbiI CCGCTC 2 cut(s) 1043, 1945
MboI GATC 8 cut(s) 222, 424, 836, 892, 1081, 1189, 1225, 1423
MflI RGATCY 1 cut(s) 1225
MhlI GDGCHC 9 cut(s) 141, 500, 557, 573, 650, 1311, 1627, 1952, 2100
MlsI TGGCCA 1 cut(s) 1786
MluCI AATT 7 cut(s) 435, 908, 1320, 1396, 1563, 1589, 1614
MluNI TGGCCA 1 cut(s) 1786
Mly113I GGCGCC 1 cut(s) 1115
MlyI GAGTC 3 cut(s) 644, 820, 1358
MmeI TCCRAC 2 cut(s) 130, 848
Mox20I TGGCCA 1 cut(s) 1786
MroI TCCGGA 2 cut(s) 774, 1559
MroNI GCCGGC 1 cut(s) 1030
MscI TGGCCA 1 cut(s) 1786
MseI TTAA 9 cut(s) 144, 252, 921, 1302, 1439, 1449, 1566, 1592, 2084
Msp20I TGGCCA 1 cut(s) 1786
MspA1I CMGCKG 2 cut(s) 1655, 2151
MspR9I CCNGG 8 cut(s) 1119, 1248, 1260, 1277, 1896, 2027, 2164, 2331
Mva1269I GAATGC 2 cut(s) 1372, 2193
MvaI CCWGG 4 cut(s) 1119, 1277, 2027, 2331
MvnI CGCG 1 cut(s) 479
NaeI GCCGGC 1 cut(s) 1032
NarI GGCGCC 1 cut(s) 1115
NciI CCSGG 4 cut(s) 1248, 1260, 1896, 2164
NcoI CCATGG 3 cut(s) 525, 758, 1164
NdeII GATC 8 cut(s) 222, 424, 836, 892, 1081, 1189, 1225, 1423
NgoMIV GCCGGC 1 cut(s) 1030
NmeAIII GCCGAG 2 cut(s) 669, 1153
NmuCI GTSAC 6 cut(s) 701, 1513, 1609, 1954, 1978, 2170
NsbI TGCGCA 1 cut(s) 290
NspI RCATGY 2 cut(s) 74, 979
PciI ACATGT 1 cut(s) 975
PcsI WCGNNNNNNNCGW 2 cut(s) 222, 357
PctI GAATGC 2 cut(s) 1372, 2193
PdiI GCCGGC 1 cut(s) 1032
PfeI GAWTC 6 cut(s) 337, 402, 472, 613, 662, 778
Pfl23II CGTACG 1 cut(s) 217
PinAI ACCGGT 1 cut(s) 1409
PleI GAGTC 3 cut(s) 643, 820, 1357
PluTI GGCGCC 1 cut(s) 1118
PmaCI CACGTG 1 cut(s) 2286
PmlI CACGTG 1 cut(s) 2286
PpsI GAGTC 3 cut(s) 643, 820, 1357
Ppu21I YACGTR 3 cut(s) 361, 2210, 2286
PpuMI RGGWCCY 1 cut(s) 1279
PscI ACATGT 1 cut(s) 975
Psp124BI GAGCTC 4 cut(s) 573, 650, 1311, 2100
Psp5II RGGWCCY 1 cut(s) 1279
Psp6I CCWGG 4 cut(s) 1117, 1275, 2025, 2329
PspCI CACGTG 1 cut(s) 2286
PspFI CCCAGC 3 cut(s) 112, 499, 1214
PspGI CCWGG 4 cut(s) 1117, 1275, 2025, 2329
PspLI CGTACG 1 cut(s) 217
PspOMI GGGCCC 2 cut(s) 553, 1948
PspPPI RGGWCCY 1 cut(s) 1279
PstI CTGCAG 1 cut(s) 2107
PstNI CAGNNNCTG 3 cut(s) 890, 1655, 1834
PsuI RGATCY 1 cut(s) 1225
PvuII CAGCTG 1 cut(s) 2151
RsaI GTAC 7 cut(s) 11, 198, 219, 314, 359, 390, 956
RsaNI GTAC 7 cut(s) 10, 197, 218, 313, 358, 389, 955
SacI GAGCTC 4 cut(s) 573, 650, 1311, 2100
SaqAI TTAA 9 cut(s) 144, 252, 921, 1302, 1439, 1449, 1566, 1592, 2084
Sau3AI GATC 8 cut(s) 222, 424, 836, 892, 1081, 1189, 1225, 1423
ScaI AGTACT 1 cut(s) 11
SchI GAGTC 3 cut(s) 644, 820, 1358
ScrFI CCNGG 8 cut(s) 1119, 1248, 1260, 1277, 1896, 2027, 2164, 2331
SduI GDGCHC 9 cut(s) 141, 500, 557, 573, 650, 1311, 1627, 1952, 2100
SfaNI GCATC 6 cut(s) 118, 316, 389, 473, 1655, 2233
SfcI CTRYAG 2 cut(s) 930, 2103
SfoI GGCGCC 1 cut(s) 1116
SinI GGWCC 3 cut(s) 1195, 1236, 1279
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 7 cut(s) 435, 908, 1320, 1396, 1563, 1589, 1614
SspDI GGCGCC 1 cut(s) 1114
SspI AATATT 2 cut(s) 1172, 1810
SspMI CTAG 2 cut(s) 546, 1469
SstI GAGCTC 4 cut(s) 573, 650, 1311, 2100
StyD4I CCNGG 8 cut(s) 1117, 1246, 1258, 1275, 1894, 2025, 2162, 2329
StyI CCWWGG 3 cut(s) 525, 758, 1164
TaaI ACNGT 5 cut(s) 196, 701, 931, 2170, 2255
TaiI ACGT 4 cut(s) 219, 363, 2212, 2288
TaqI TCGA 6 cut(s) 135, 168, 176, 348, 457, 494
TasI AATT 7 cut(s) 435, 908, 1320, 1396, 1563, 1589, 1614
TatI WGTACW 1 cut(s) 9
TfiI GAWTC 6 cut(s) 337, 402, 472, 613, 662, 778
Tru1I TTAA 9 cut(s) 144, 252, 921, 1302, 1439, 1449, 1566, 1592, 2084
Tru9I TTAA 9 cut(s) 144, 252, 921, 1302, 1439, 1449, 1566, 1592, 2084
TscAI CASTG 4 cut(s) 267, 514, 934, 1959
TseFI GTSAC 6 cut(s) 701, 1513, 1609, 1954, 1978, 2170
TseI GCWGC 7 cut(s) 205, 897, 900, 1357, 1700, 1797, 2111
Tsp45I GTSAC 6 cut(s) 701, 1513, 1609, 1954, 1978, 2170
TspDTI ATGAA 4 cut(s) 201, 782, 1004, 1509
TspGWI ACGGA 5 cut(s) 235, 453, 1039, 1301, 2253
TspRI CASTG 4 cut(s) 267, 514, 934, 1959
VpaK11BI GGWCC 3 cut(s) 1195, 1236, 1279
XapI RAATTY 2 cut(s) 435, 1396
XceI RCATGY 2 cut(s) 74, 979
XspI CTAG 2 cut(s) 546, 1469
ZrmI AGTACT 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.