AT4G00680

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0021859
Physical Location & Seq
Reverse (-)
279596 .. 281072
1477 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G00680.1

Sequence Viewer

Length: 423 bp
ATGGCTAATTCAGCGTCTGGGATGCATGTGAATGATGAATGCAAGATTAAGTTCTTAGAGTTGAAAGCAAAGAGGACTTACAGGTTCATTGTGTTCAAAATTGATGAGAAGGCACAGCAAGTGCAAATAGAAAAGCTTGGGAATCCAGAAGAAACTTACGACGATTTTACCAGCTCTATCCCCGATGACGAATGCCGATACGCTGTCTACGATTTTGATTTCACCACAGAGGACAATTGTCAGAAGAGCAAGATCTTTTTCATCGCGTGGTCACCTGATACATCGAGAGTTCGGAGTAAGATGTTGTATGCAAGCTCAAAAGACAGGTTCAAGAGAGAAATGGAAGGAATTCAAGTTGAATTGCAAGCAACTGATCCTAGTGAGATGAGCCTTGACATCATCAAAGGACGACTCAATCTCTGA

Protein Analysis

140

Amino Acids

16.32

Weight (kDa)

5.12

Isoelectric Point (pI)

47.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 7 - 138 1.4e-43 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021859)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00680 AT4G00680
rosa_samantha Rh3AG088400 Rh3BG091300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 207
AccII CGCG 1 cut(s) 266
AclWI GGATC 1 cut(s) 368
AcsI RAATTY 1 cut(s) 348
AgsI TTSAA 5 cut(s) 64, 97, 331, 353, 359
AluBI AGCT 3 cut(s) 136, 174, 315
AluI AGCT 3 cut(s) 136, 174, 315
AlwI GGATC 1 cut(s) 368
AlwNI CAGNNNCTG 1 cut(s) 17
ApoI RAATTY 1 cut(s) 348
Asp700I GAANNNNTTC 1 cut(s) 348
AsuHPI GGTGA 2 cut(s) 214, 264
BfaI CTAG 1 cut(s) 378
BglII AGATCT 1 cut(s) 252
BmsI GCATC 1 cut(s) 12
BoxI GACNNNNGTC 1 cut(s) 237
BseGI GGATG 1 cut(s) 27
Bsh1236I CGCG 1 cut(s) 266
BsmI GAATGC 2 cut(s) 44, 197
Bsp143I GATC 2 cut(s) 252, 373
BspFNI CGCG 1 cut(s) 266
BspPI GGATC 1 cut(s) 368
BspQI GCTCTTC 1 cut(s) 239
BssMI GATC 2 cut(s) 252, 373
Bst6I CTCTTC 1 cut(s) 239
BstC8I GCNNGC 2 cut(s) 313, 366
BstDEI CTNAG 1 cut(s) 55
BstEII GGTNACC 1 cut(s) 270
BstF5I GGATG 1 cut(s) 27
BstFNI CGCG 1 cut(s) 266
BstKTI GATC 2 cut(s) 255, 376
BstMBI GATC 2 cut(s) 252, 373
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNSI RCATGY 1 cut(s) 29
BstPAI GACNNNNGTC 1 cut(s) 237
BstPI GGTNACC 1 cut(s) 270
BstUI CGCG 1 cut(s) 266
BstX2I RGATCY 1 cut(s) 252
BstYI RGATCY 1 cut(s) 252
BtgZI GCGATG 1 cut(s) 247
BtsCI GGATG 1 cut(s) 27
Cac8I GCNNGC 2 cut(s) 313, 366
CaiI CAGNNNCTG 1 cut(s) 17
CseI GACGC 1 cut(s) 3
CviAII CATG 1 cut(s) 26
CviJI RGCY 5 cut(s) 5, 136, 174, 315, 390
CviKI_1 RGCY 5 cut(s) 5, 136, 174, 315, 390
DdeI CTNAG 1 cut(s) 55
DpnI GATC 2 cut(s) 254, 375
DpnII GATC 2 cut(s) 252, 373
Eam1104I CTCTTC 1 cut(s) 239
EarI CTCTTC 1 cut(s) 239
Eco91I GGTNACC 1 cut(s) 270
EcoO65I GGTNACC 1 cut(s) 270
EcoRI GAATTC 1 cut(s) 348
EcoT22I ATGCAT 1 cut(s) 27
FaeI CATG 1 cut(s) 29
FaiI YATR 2 cut(s) 27, 309
FatI CATG 1 cut(s) 25
FblI GTMKAC 1 cut(s) 207
FokI GGATG 1 cut(s) 34
FspBI CTAG 1 cut(s) 378
HgaI GACGC 1 cut(s) 3
Hin1II CATG 1 cut(s) 29
HindIII AAGCTT 1 cut(s) 134
HinfI GANTC 2 cut(s) 142, 411
HphI GGTGA 2 cut(s) 214, 264
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 3 cut(s) 243, 294, 422
Hpy188III TCNNGA 3 cut(s) 146, 285, 331
Hpy8I GTNNAC 1 cut(s) 208
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 2 cut(s) 103, 338
HpyCH4V TGCA 5 cut(s) 25, 42, 124, 311, 364
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 1 cut(s) 55
Hsp92II CATG 1 cut(s) 29
Kzo9I GATC 2 cut(s) 252, 373
LguI GCTCTTC 1 cut(s) 239
LpnPI CCDG 6 cut(s) 3, 67, 159, 184, 288, 310
LweI GCATC 1 cut(s) 12
MaeI CTAG 1 cut(s) 378
MaeIII GTNAC 1 cut(s) 270
MalI GATC 2 cut(s) 254, 375
MboI GATC 2 cut(s) 252, 373
MboII GAAGA 2 cut(s) 161, 256
MfeI CAATTG 1 cut(s) 235
MflI RGATCY 1 cut(s) 252
MluCI AATT 5 cut(s) 7, 99, 235, 348, 359
MlyI GAGTC 1 cut(s) 405
MnlI CCTC 2 cut(s) 66, 223
Mph1103I ATGCAT 1 cut(s) 27
MroXI GAANNNNTTC 1 cut(s) 348
MseI TTAA 1 cut(s) 48
MslI CAYNNNNRTG 1 cut(s) 30
MunI CAATTG 1 cut(s) 235
Mva1269I GAATGC 2 cut(s) 44, 197
MvnI CGCG 1 cut(s) 266
MwoI GCNNNNNNNGC 1 cut(s) 11
NdeII GATC 2 cut(s) 252, 373
NlaIII CATG 1 cut(s) 29
NmuCI GTSAC 1 cut(s) 270
NsiI ATGCAT 1 cut(s) 27
NspI RCATGY 1 cut(s) 29
PciSI GCTCTTC 1 cut(s) 239
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PctI GAATGC 2 cut(s) 44, 197
PdmI GAANNNNTTC 1 cut(s) 348
PfeI GAWTC 1 cut(s) 142
PleI GAGTC 1 cut(s) 405
PpsI GAGTC 1 cut(s) 405
PshAI GACNNNNGTC 1 cut(s) 237
PspEI GGTNACC 1 cut(s) 270
PstNI CAGNNNCTG 1 cut(s) 17
PsuI RGATCY 1 cut(s) 252
RseI CAYNNNNRTG 1 cut(s) 30
SapI GCTCTTC 1 cut(s) 239
SaqAI TTAA 1 cut(s) 48
Sau3AI GATC 2 cut(s) 252, 373
SchI GAGTC 1 cut(s) 405
SetI ASST 6 cut(s) 86, 138, 176, 277, 317, 329
SfaNI GCATC 1 cut(s) 12
SmiMI CAYNNNNRTG 1 cut(s) 30
Sse9I AATT 5 cut(s) 7, 99, 235, 348, 359
SspMI CTAG 1 cut(s) 378
TaqI TCGA 1 cut(s) 284
TasI AATT 5 cut(s) 7, 99, 235, 348, 359
TfiI GAWTC 1 cut(s) 142
Tru1I TTAA 1 cut(s) 48
Tru9I TTAA 1 cut(s) 48
TseFI GTSAC 1 cut(s) 270
Tsp45I GTSAC 1 cut(s) 270
TspDTI ATGAA 3 cut(s) 51, 76, 250
XapI RAATTY 1 cut(s) 348
XceI RCATGY 1 cut(s) 29
XmiI GTMKAC 1 cut(s) 207
XmnI GAANNNNTTC 1 cut(s) 348
XspI CTAG 1 cut(s) 378
Zsp2I ATGCAT 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.